A user centered design methodology for functional and smart garments

Bioswim® is a multidisciplinary project that is developing a wireless full-body monitored swimsuit with the aim of increasing swimmer performance. The different points of view of the multiple working groups brought to the project a different vision that expanded the primary aim of the project to a universal level where the application of the instrumented suit for other objectives could be a real fact. Given that most research user-centred methodologies, although straying from the user needs, are in the end actually focused on the final design product, a different approach to the problem had to be found by the working group: to have the user’s input as a significant and central part of the project. Within the project, a new methodology - the User Centered Layered Methodology (UCLM), was designed, implemented and tested.Fundação para a Ciência e a Tecnologia (FCT) - Projeto Bioswi

A 2.4-GHz low-power/low-voltage wireless plug-and-play module for EEG applications

This paper presents a plug-and-play module for wireless electroencephalogram (EEG) applications. The wireless module is composed by an electrode, processing electronics, a radio-frequency (RF) transceiver, and an associated antenna. The RF transceiver was fabricated in the UMC RF 0.18 mum CMOS process, and operates in the 2.4-GHz ISM band. The receiver has a sensitivity of -60 dBm and a power consumption of 6.3 mW from a 1.8 V supply. The transmitter delivers an output power of 0 dBm with a power consumption of 11.2 mW, for a range of 10 m. It is also presented the electrical performance and comparison between different electrodes for EEG applications, namely sputtered titanium nitride (TiN) electrodes, standard sintered silver/silver chloride (Ag/AgCl) ring electrodes and sputtered iridium oxide (IrO2) electrodes. The experimental results show a better performance of the sputtered IrO2 electrodes compared with the standard sintered Ag/AgCl ring electrodes. These results promise a new opportunity for the application of a dry IrO2 electrodes in wireless modules for using in a wearable EEG braincap. These wireless EEG modules will allow patients to wear a brain cap and maintain their mobility, while simultaneously having their electrical brain activity monitored

Textile sensors for ECG and respiratory frequency on swimsuits

Swimming constitutes one of the most demanding sports regarding technique. Years of training are necessary to master each one of the four styles. An important improvement and help for trainers would be a swimsuit that could provide information during training. This paper presents the research undertaken to develop textile sensors that will be used in a swimsuit. This paper will address ECG and respiratory frequency sensors and respective signals. The behaviour of the proposed sensors in different conditions (dry and wet environments) will be presented and discussed. The influence of movement on the signal quality and further interpretation, both by the muscular electrical signals as well as by the displacement of the electrodes, will be addressed. Other very important issue in swimming is drag. One approach that can reduce total drag consists in using compression. However, compressed fabrics will most likely modify the textile sensors’Fundação para a Ciência e a Tecnologia (FCT) - PTDC/EEA-ELC/70803/200

Study of vital sign monitoring with textile sensors in swimming pool environment

This paper presents the results of a series of experiments aiming at the optimisation of vital sign monitoring using textile electrodes to be used in a swimsuit. The swimsuit will integrate sensors for the measurement of several physiological and biomechanical signals; this paper will focus on ECG and respiratory movement analysis. The data obtained is mainly intended to provide tools for evaluation of high-performance swimmers, although applications can be derived for leisure sports and other situations. A comparison between electrodes based on different materials and structures, behaviour in dry and wet environments, as well as the behavior in different extension states, will be presented. The influence of movement on the signal quality, both by the muscular electrical signals as well as by the displacement of the electrodes, will be discussed. The final objective is the integration of the electrodes in the swimsuit by knitting them directly in the suit’s fabric in a seamless knitting machineFundação para a Ciência e a Tecnologia (FCT) - PTDC/EEAELC/70803/200

Advancements in on-line monitoring and control of parameters in knitting and sewing processes

This paper presents a summary of the developments in process control in textile processes at the University of Minho, by a multidisciplinary research group involving three different departments (Textile, lectronic and Mechanical Engineering). The studies target the automatic process parameter monitoring and control in the areas of industrial sewing and knitting.Fundação para a Ciência e a Tecnologia (FCT)

Influence of processing conditions on the β-Phase content of PVDF samples produced by tape extrusion

This research team has been working during the last couple of years on the development of industrial scalable technologies, based on the extrusion processing technique, for the prodution of piezoeletric products. This specific work comprises a study on the effect of tape extrusion processing conditions on the content of PVDF β-phase. For this purpose the extruded samples were uniaxially stretched up to ratios of 6, at 80ºC and 120ºC, being the β-phase content analysed by FTIR. Subsequently, the samples were corona poled and their piezoelectric coefficient d33 was measured. The results obtained allowed to conclude that the extrusion processing variables, with the exception of the stretching temperature and ratio, do not have a strong influence on the β-phase content and piezoelectric response of PVDF.Fundação para a Ciência e a Tecnologia (FCT

A Survey on Long-Range Wide-Area Network Technology Optimizations

Long-Range Wide-Area Network (LoRaWAN) enables flexible long-range service communications with low power consumption which is suitable for many IoT applications. The densification of LoRaWAN, which is needed to meet a wide range of IoT networking requirements, poses further challenges. For instance, the deployment of gateways and IoT devices are widely deployed in urban areas, which leads to interference caused by concurrent transmissions on the same channel. In this context, it is crucial to understand aspects such as the coexistence of IoT devices and applications, resource allocation, Media Access Control (MAC) layer, network planning, and mobility support, that directly affect LoRaWAN’s performance.We present a systematic review of state-of-the-art works for LoRaWAN optimization solutions for IoT networking operations. We focus on five aspects that directly affect the performance of LoRaWAN. These specific aspects are directly associated with the challenges of densification of LoRaWAN. Based on the literature analysis, we present a taxonomy covering five aspects related to LoRaWAN optimizations for efficient IoT networks. Finally, we identify key research challenges and open issues in LoRaWAN optimizations for IoT networking operations that must be further studied in the future

Haemocompatibility of iron oxide nanoparticles synthesized for theranostic applications: a high-sensitivity microfluidic tool

The poor heating efficiency of the most reported magnetic nanoparticles (MNPs), allied to the lack of comprehensive biocompatibility and haemodynamic studies, hampers the spread of multifunctional nanoparticles as the next generation of therapeutic bio-agents in medicine. The present work reports the synthesis and characterization, with special focus on biological/toxicological compatibility, of superparamagnetic nanoparticles with diameter around 18 nm, suitable for theranostic applications (i.e. simultaneous diagnosis and therapy of cancer). Envisioning more insights into the complex nanoparticle-red blood cells (RBCs) membrane interaction, the deformability of the human RBCs in contact with magnetic nanoparticles (MNPs) was assessed for the first time with a microfluidic extensional approach, and used as an indicator of haematological disorders in comparison with a conventional haematological test, i.e. the haemolysis analysis. Microfluidic results highlight the potential of this microfluidic tool over traditional haemolysis analysis, by detecting small increments in the rigidity of the blood cells, when traditional haemotoxicology analysis showed no significant alteration (haemolysis rates lower than 2 %). The detected rigidity has been predicted to be due to the wrapping of small MNPs by the bilayer membrane of the RBCs, which is directly related to MNPs size, shape and composition. The proposed microfluidic tool adds a new dimension into the field of nanomedicine, allowing to be applied as a highsensitivity technique capable of bringing a better understanding of the biological impact of nanoparticles developed for clinical applications.This work was financially supported by: Project POCI-01-0145-FEDER-006984 – Associate Laboratory J Nanopart Res (2016) 18:194 Page 15 of 17 194 123 LSRE-LCM funded by FEDER funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalizac¸a˜o (POCI) – and by national funds through FCT - Fundac¸a˜o para a Cieˆncia e a Tecnologia. R.O.R. acknowledges the Ph.D. scholarship SFRH/BD/97658/2013 Granted by FCT. A.M.T.S acknowledges the FCT Investigator 2013 Programme (IF/01501/ 2013), with financing from the European Social Fund and the Human Potential Operational Programme. M.B. would like to thank ERDF (European Regional Development Fund) under grant PO Norte CCDR-N/ON.2 Programme. J.G. also thanks the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 600375.info:eu-repo/semantics/publishedVersio

Androgen responsive intronic non-coding RNAs

BACKGROUND: Transcription of large numbers of non-coding RNAs originating from intronic regions of human genes has been recently reported, but mechanisms governing their biosynthesis and biological functions are largely unknown. In this work, we evaluated the existence of a common mechanism of transcription regulation shared by protein-coding mRNAs and intronic RNAs by measuring the effect of androgen on the transcriptional profile of a prostate cancer cell line. RESULTS: Using a custom-built cDNA microarray enriched in intronic transcribed sequences, we found 39 intronic non-coding RNAs for which levels were significantly regulated by androgen exposure. Orientation-specific reverse transcription-PCR indicated that 10 of the 13 were transcribed in the antisense direction. These transcripts are long (0.5–5 kb), unspliced and apparently do not code for proteins. Interestingly, we found that the relative levels of androgen-regulated intronic transcripts could be correlated with the levels of the corresponding protein-coding gene (asGAS6 and asDNAJC3) or with the alternative usage of exons (asKDELR2 and asITGA6) in the corresponding protein-coding transcripts. Binding of the androgen receptor to a putative regulatory region upstream from asMYO5A, an androgen-regulated antisense intronic transcript, was confirmed by chromatin immunoprecipitation. CONCLUSION: Altogether, these results indicate that at least a fraction of naturally transcribed intronic non-coding RNAs may be regulated by common physiological signals such as hormones, and further corroborate the notion that the intronic complement of the transcriptome play functional roles in the human gene-expression program

Performance and modeling of Ni(II) adsorption from low concentrated wastewater on carbon microspheres prepared from tangerine peels by FeCl3-assisted hydrothermal carbonization

The presence of heavy metals in the environment as a consequence of human activity is an issue that has caught the attention of researchers to find wastewater treatment solutions, such as adsorption. In this work, hydrochars and activated carbon microspheres are prepared from tangerine peels as carbon precursor and FeCl3 as activating and structure-directing agent in the hydrothermal carbonization, allowing to obtain hydrochar microspheres ranging from 50 to 3615 nm. In addition, a pyrochar was prepared by pyrolysis of the same precursor. The activated carbon shows the highest surface area (SBET up to 287 m2 g–1), but the basicity of the pyrochar (1.83 mmol g 1, SBET = 104 m2 g–1) was determinant in the adsorption of Ni, being considered the carbon-based material with the highest uptake capacity of Ni. Isotherm and kinetic adsorption of Ni on the most representative activated carbon microsphere, pyrochar and hydrochar microsphere are assessed by 10 and 7 models, respectively.The authors are grateful to the FCT (Foundation for Science and Technology, Portugal) and FEDER (European Regional Development Fund) under Programme PT2020 for financial support to CIMO (UIDB/00690/2020). We would also like to thank the scientific collaboration under Base- UIDB/50020/2020 and Programmatic- UIDP/50020/2020 funding of LSRE-LCM, and LA/P/0045/2020 funding of ALiCE, funded by national funds through FCT and MCTES (Ministério da Ciência, Tecnologia e Ensino Superior, Portugal) by PIDDAC (Programa de Investimentos e Despesas de Desenvolvimento da Administraç˜ao Central, Portugal). Fernanda F. Roman and Adriano dos Santos Silva acknowledge the national funding by FCT and MIT (Massachusetts Institute of Technology, USA), and the ESF (European Social Fund) for individual research grants with reference numbers of SFRH/BD/143224/2019 and SFRH/BD/151346/2021, respectively.info:eu-repo/semantics/publishedVersio