3,130 research outputs found
The essential and enigmatic role of ABC transporters in Bt resistance of Noctuids and other insect pests of agriculture
In the last ten years, ABC transporters have emerged as unexpected yet significant contributors to pest resistance to insecticidal pore-forming proteins from Bacillus thuringiensis (Bt). Evidence includes the presence of mutations in resistant insects, heterologous expression to probe interactions with the three-domain Cry toxins, and CRISPR/Cas9 knockouts. Yet the mechanisms by which ABC transporters facilitate pore formation remain obscure. The three major classes of Cry toxins used in agriculture have been found to target the three major classes of ABC transporters, which requires a mechanistic explanation. Many other families of bacterial pore-forming toxins exhibit conformational changes in their mode of action, which are not yet described for the Cry toxins. Three-dimensional structures of the relevant ABC transporters, the multimeric pore in the membrane, and other proteins that assist in the process are required to test the hypothesis that the ATP-switch mechanism provides a motive force that drives Cry toxins into the membrane. Knowledge of the mechanism of pore insertion will be required to combat the resistance that is now evolving in field populations of insects, including noctuids
Impacts of Exotic Invasive Vines on the Ecology and Reproduction of the Endangered Trillium Reliquum
Trillium reliquum Freeman is an endangered plant restricted to 20 counties in three states that is imperilled by habitat loss and encroachment from the exotic invasive vines, kudzu (Pueraria montana Ohwi) and Japanese honeysuckle (Lonicera japonica Thunb.) My study examines the impact of kudzu and honeysuckle on native plant communities, T. reliquum population dynamics and T. reliquum reproduction
Lorentz Violation and Synchrotron Radiation
We consider the radiation emitted by an ultrarelativistic charged particle
moving in a magnetic field, in the presence of an additional Lorentz-violating
interaction. In contrast with prior work, we treat a form of Lorentz violation
that is represented by a renormalizable operator. Neglecting the radiative
reaction force, the particle's trajectory can be determined exactly. The
resulting orbit is generally noncircular and does not lie in the place
perpendicular to the magnetic field. We do not consider any Lorentz violation
in the electromagnetic sector, so the radiation from the accelerated charge can
be determined by standard means, and the radiation spectrum will exhibit a
Lorentz-violating directional dependence. Using data on emission from the Crab
nebula, we can set a bound on a particular combination of Lorentz-violating
coefficients at the level.Comment: 14 page
Intraspecific and interstage similarities in host-plant preference in the diamondback moth (Lepidoptera: Plutellidae)
The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), is an important insect pest of cruciferous crops. Understanding its preference patterns can lead to more efficient management methods, such as trap crops. Several strains of P. xylostella were used to test whether there were differences in oviposition preference in a four-choice setting, on abaxial versus adaxial leaf surfaces in 28 different plant species, and on substrates with different concentrations of sinigrin (allylglucosinolate). Additionally, the larval preference of P. xylostella was studied with 17 plant species of known glucosinolate content that were compared to Arabidopsis thaliana L. in two-choice tests. Our research shows that the diet on which P. xylostella has fed hardly affects multiple-choice host-plant preference, abaxial and adaxial oviposition preference, or oviposition response to pure glucosinolates. Our study also shows that glucosinolate content affects larval preference, which together with the known correlation between glucosinolate content and P. xylostella oviposition, indicates that crops with high glucosinolate content could be more susceptible to damage by P. xylostella than crops with low glucosinolate content. These findings are discussed in regards to their significance in the management of P. xylostella
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