988 research outputs found

    Characterisation of polymorphic microsatellite loci in the freshwater bryozoan Fredericella sultana

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    Eight polymorphic microsatellite loci were isolated from massively parallel next-generation sequencing data and tested in three populations (74 individuals) of the colonial freshwater bryozoan Fredericella sultana. Up to 13 alleles per locus were found and all loci were polymorphic in all populations. Minimum of three loci were sufficient to distinguish all unique multilocus genotypes. These highly variable markers are suitable for clonal identity assignment based on unique multilocus genotypes and provide tools for resolving fine scale population structure in a species characterised by clonal, vegetative growth and asexual reproductio

    Resource-efficient purification of acidic multi-metal process water by means of anionic nanofibrillated cellulose

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    Treatment of acidic mining water (MW) with industrial minerals and alkaline chemicals requires utilisation of unrenewable raw materials and produces disposable inorganic sludges of no further use. We investigated the efficiency of bio-based anionic nanofibrillated cellulose (NFC) to purify authentic MW high in metals and sulphate. In a short-term (10 min) adsorption experiment, highly acidic (pH 3.2) multi-metal process water was treated with anionic NFC gels differing in their consistency (1.1%, 1.4% and 1.8% wow) at three sorbent-to-solution ratios. To unravel the purification efficiency of the NFC gels, MW was treated stepwise with a set of fresh NFC gels in three sequential batches. Each treated solution was filtrated before pH measurement and analysis for the NFC-induced changes in the metal and sulphate concentrations. All NFC gels efficiently co-adsorbed metals and sulphate and decreased the acidity of MW. Depending on the dosage, a triplicated treatment with the NFC gels removed as much as 32-75% of metal cations and 34-75% of sulphate anions. The retention of metals highly exceeded the amount of carboxyl groups in the sorbent Thus, we concluded that, instead of electrostatic adsorption, the retention took place through formation of covalent metal-NFC complexes. The subsequent surplus in positive total charge formed on the NFC-surface, in turn, enabled electrostatic co-adsorption of sulphate anions. The mutual interactions between cellulose nanofibrils in the NFC gel weakened with decreasing consistency, which promoted the accessibility of the sorption sites. This improved the purification efficiency while decreasing the demand for cellulosic raw material. We concluded that anionic NFC could potentially serve as a multifunctional and resource-efficient purification agent in the treatment of acidic process waters of high ionic strength. Ideally, the elements retained could be liberated and recycled elsewhere. (C) 2018 Elsevier Ltd. All rights reserved.Peer reviewe

    Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease

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    0000-0001-7279-715X© Springer Science+Business Media Dordrecht 2014. The attached document is the authors' final accepted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it

    Low attentional engagement makes attention network activity susceptible to emotional interference

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    The aim of this study was to investigate whether emotion-attention interaction depends on attentional engagement. To investigate emotional modulation of attention network activation, we used a functional MRI paradigm consisting of a visuospatial attention task with either frequent (high-engagement) or infrequent (low-engagement) targets and intermittent emotional or neutral distractors. The attention task recruited a bilateral frontoparietal network with no emotional interference on network activation when the attentional engagement was high. In contrast, when the attentional engagement was low, the unpleasant stimuli interfered with the activation of the frontoparietal attention network, especially in the right hemisphere. This study provides novel evidence for low attentional engagement making attention control network activation susceptible to emotional interference. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.Fil: Exposito, Veronica. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Tampere; FinlandiaFil: Pickard, Natasha. California State University; Estados UnidosFil: Solbakk, Anne-Kristin. University of Oslo; NoruegaFil: Ogawa, Keith H.. Saint Mary's College Of California; Estados UnidosFil: Knight, Robert T.. California State University; Estados UnidosFil: Hartikainen, Kaisa M.. Universidad de Tampere; Finlandi

    First microsatellite loci of the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (PKD)

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    0000-0001-7279-715XCopyright © 2015 Inter-Research. The attached document is the authors' final accepted/submitted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it

    Tetracapsuloides bryosalmonae abundance in river water

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    The file attached is the Accepted/final draft post-refereeing version of the article

    Bryozoan stable carbon and hydrogen isotopes: relationships between the isotopic composition of zooids, statoblasts and lake water

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    0000-0001-7279-715X© Springer International Publishing Switzerland 2015. The attached document is the authors' final accepted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it

    Retention of metal and sulphate ions from acidic mining water by anionic nanofibrillated cellulose

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    We carried out an adsorption experiment to investigate the ability of anionic nanofibrillated cellulose (NFC) to retain metal and SO42- ions from authentic highly acidic (pH 3.2) mining water. Anionic NFC gels of different consistencies (1.1%, 1.4-% and 1.8-% w/w) were allowed to react for 10 min with mining water, after which NFC-induced changes in the metal and SO42- concentrations of the mining water were determined. The sorption capacities of the NFC gels were calculated as the difference between the element concentrations in the untreated and NFC-treated mining water samples. All the NFCs efficiently co-adsorbed both metals and SO42-. The retention of metals was concluded to take place through formation of metal-ligand complexes. The reaction between the NFC ligand and the polyvalent cations renders the cellulose nanofibrils positively charged and, thus, able to retain SO42- electrostatically. Adsorption capacity of the NFC gels substantially increased upon decreasing DM content as a result of the dilution-induced weakening of the mutual interactions between individual cellulose nanofibrils. This outcome reveals that the dilution of the NFC gel not only increases its purification capacity but also reduces the demand for cellulosic raw material. These results suggest that anionic NFC made of renewable materials serves as an environmentally sound and multifunctional purification agent for acidic multimetal mining waters or AMDs of high ionic strength. Unlike industrial minerals traditionally used to precipitate valuable metals from acidic mining effluents before their permanent disposal from the material cycle, NFC neither requires mining of unrenewable raw materials nor produces inorganic sludges. (C) 2017 Elsevier B.V. All rights reserved.Peer reviewe

    Vertical transmission of Tetracapsuloides bryosalmonae (Myxozoa), the causative agent of salmonid proliferative kidney disease

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    license: Copyright © Cambridge University Press 2013 0000-0001-7279-715Xlicense: Copyright © Cambridge University Press 2013. The attached document is the authors' final accepted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it

    Gene Duplication and Gain in the Trematode Atriophallophorus winterbourni Contributes to Adaptation to Parasitism.

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    Gene duplications and novel genes have been shown to play a major role in helminth adaptation to a parasitic lifestyle because they provide the novelty necessary for adaptation to a changing environment, such as living in multiple hosts. Here we present the de novo sequenced and annotated genome of the parasitic trematode Atriophallophorus winterbourni and its comparative genomic analysis to other major parasitic trematodes. First, we reconstructed the species phylogeny, and dated the split of A. winterbourni from the Opisthorchiata suborder to approximately 237.4 Ma (±120.4 Myr). We then addressed the question of which expanded gene families and gained genes are potentially involved in adaptation to parasitism. To do this, we used hierarchical orthologous groups to reconstruct three ancestral genomes on the phylogeny leading to A. winterbourni and performed a GO (Gene Ontology) enrichment analysis of the gene composition of each ancestral genome, allowing us to characterize the subsequent genomic changes. Out of the 11,499 genes in the A. winterbourni genome, as much as 24% have arisen through duplication events since the speciation of A. winterbourni from the Opisthorchiata, and as much as 31.9% appear to be novel, that is, newly acquired. We found 13 gene families in A. winterbourni to have had more than ten genes arising through these recent duplications; all of which have functions potentially relating to host behavioral manipulation, host tissue penetration, and hiding from host immunity through antigen presentation. We identified several families with genes evolving under positive selection. Our results provide a valuable resource for future studies on the genomic basis of adaptation to parasitism and point to specific candidate genes putatively involved in antagonistic host-parasite adaptation
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