Molecular Genetic Typing of Staphylococcus aureus from Cows, Goats, Sheep, Rabbits and Chickens

End of project reportsS. aureus can also cause a number of infections in animals such as tick-associated pyaemia in lambs, staphylococcosis in rabbits, septicaemia, abscesses and chondronecrosis in chickens and pneumonia and osteomyelitis complex in turkeys. S. aureus is the most frequent cause of bovine mastitis, a disease that is of economic importance worldwide (Beck et al., 1992). Typically staphylococcal mastitis is chronic in nature, with subclinical mastitis being the most common form

Population and Virulence Factor Analysis of Staphylococcus aureus from Bovine Mastitis.

End of Project ReportsStaphylococcus aureus is a major cause of bovine mastitis and the disease is responsible for substantial economic losses in the dairy industry world-wide. A large number of commonly accepted virulence factors are associated with S. aureus but it is yet to be elucidated which of these are important for infection of the bovine udder. A rational and effective strategy for the control of intramammary infections may need to be directed against clones of S. aureus that commonly cause disease. The objective of this study was to characterise the genetic variance of S. aureus isolate populations from infected udders in Ireland using RAPD-PCR, ribotyping and multilocus enzyme electrophoresis (MLEE). Similar S. aureus isolates collected in the USA were also typed in order to compare strain differences in staphylococcal populations in a different environment. Phenotypic diversity based on a number of presumed virulence factors together with antibiotic sensitivity was examined and correlations between phenotype and genotype were identified, if present. In addition, a pathogenicity island encoding multiple superantigens was completely sequenced and characterised. Knockout mutants of these superantigens were also constructed and in vitro functional analysis performed. Laboratory animal experiments (mice and rabbits) were used to study the relative pathogenicity of individual staphylococcal strains (mice) and also to measure the immunological responses after prolonged exposure to the predominant strains (rabbits)

Supplementary Data for “Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect \u3ci\u3eChlorella\u3c/i\u3e Pbi”: Appendix B: Gene Names M001L through M807R

Appendix B: Gene Names M001L through M807R Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect \u3ci\u3eChlorella\u3c/i\u3e Pbi

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP–glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that approximately 82% of the genes are present in all three viruses. Supplementary data to accompany this article is archived in this repository as 4 separate documents

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect \u3ci\u3eChlorella\u3c/i\u3e Pbi

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP–glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that approximately 82% of the genes are present in all three viruses. Supplementary data to accompany this article is archived in this repository as 4 separate documents

Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon \u3ci\u3eAcanthocystis turfacea\u3c/i\u3e

Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3.83, that is a symbiont in the heliozoon A. turfacea. The 288,047-bp ATCV-1 genome is the first virus to be sequenced that infects Chlorella SAG 3.83. ATCV-1 contains 329 putative protein-encoding and 11 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands and intergenic space is minimal. Thirty-four percent of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. For example, these unique gene products include ribonucleoside-triphosphate reductase, dTDP-D-glucose 4,6 dehydratase, potassium ion transporter, aquaglyceroporin, and mucindesulfating sulfatase. Comparison of ATCV-1 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that about 80% of the ATCV-1 genes are present in PBCV-1

Regional coherence evaluation in mild cognitive impairment and Alzheimer's disease based on adaptively extracted magnetoencephalogram rhythms

This study assesses the connectivity alterations caused by Alzheimer's disease (AD) and mild cognitive impairment (MCI) in magnetoencephalogram (MEG) background activity. Moreover, a novel methodology to adaptively extract brain rhythms from the MEG is introduced. This methodology relies on the ability of empirical mode decomposition to isolate local signal oscillations and constrained blind source separation to extract the activity that jointly represents a subset of channels. Inter-regional MEG connectivity was analysed for 36 AD, 18 MCI and 26 control subjects in δ, θ, α and β bands over left and right central, anterior, lateral and posterior regions with magnitude squared coherence—c(f). For the sake of comparison, c(f) was calculated from the original MEG channels and from the adaptively extracted rhythms. The results indicated that AD and MCI cause slight alterations in the MEG connectivity. Computed from the extracted rhythms, c(f) distinguished AD and MCI subjects from controls with 69.4% and 77.3% accuracies, respectively, in a full leave-one-out cross-validation evaluation. These values were higher than those obtained without the proposed extraction methodology

HD 101088, An Accreting 14 AU Binary in Lower Centaurus Crux With Very Little Circumstellar Dust

We present high resolution (R=55,000) optical spectra obtained with MIKE on the 6.5 m Magellan Clay Telescope as well as Spitzer MIPS photometry and IRS low resolution (R~60) spectroscopy of the close (14 AU separation) binary, HD 101088, a member of the ~12 Myr old southern region of the Lower Centaurus Crux (LCC) subgroup of the Scorpius-Centaurus OB association. We find that the primary and/or secondary is accreting from a tenuous circumprimary and/or circumsecondary disk despite the apparent lack of a massive circumbinary disk. We estimate a lower limit to the accretion rate of > 1x10^-9 solar masses per year, which our multiple observation epochs show varies over a timescale of months. The upper limit on the 70 micron flux allows us to place an upper limit on the mass of dust grains smaller than several microns present in a circumbinary disk of 0.16 moon masses. We conclude that the classification of disks into either protoplanetary or debris disks based on fractional infrared luminosity alone may be misleading.Comment: 8 pages, 2 figures, ApJ accepte