915 research outputs found
Attenuation of doxorubicin-induced cardiac injury by mitochondrial glutaredoxin 2
AbstractWhile the cardiotoxicity of doxorubicin (DOX) is known to be partly mediated through the generation of reactive oxygen species (ROS), the biochemical mechanisms by which ROS damage cardiomyocytes remain to be determined. This study investigates whether S-glutathionylation of mitochondrial proteins plays a role in DOX-induced myocardial injury using a line of transgenic mice expressing the human mitochondrial glutaredoxin 2 (Glrx2), a thiotransferase catalyzing the reduction as well as formation of protein–glutathione mixed disulfides, in cardiomyocytes. The total glutaredoxin (Glrx) activity was increased by 76% and 53 fold in homogenates of whole heart and isolated heart mitochondria of Glrx2 transgenic mice, respectively, compared to those of nontransgenic mice. The expression of other antioxidant enzymes, with the exception of glutaredoxin 1, was unaltered. Overexpression of Glrx2 completely prevents DOX-induced decreases in NAD- and FAD-linked state 3 respiration and respiratory control ratio (RCR) in heart mitochondria at days 1 and 5 of treatment. The extent of DOX-induced decline in left ventricular function and release of creatine kinase into circulation at day 5 of treatment was also greatly attenuated in Glrx2 transgenic mice. Further studies revealed that heart mitochondria overexpressing Glrx2 released less cytochrome c than did controls in response to treatment with tBid or a peptide encompassing the BH3 domain of Bid. Development of tolerance to DOX toxicity in transgenic mice is also associated with an increase in protein S-glutathionylation in heart mitochondria. Taken together, these results imply that S-glutathionylation of heart mitochondrial proteins plays a role in preventing DOX-induced cardiac injury
Attenuation of Doxorubicin-Induced Cardiac Injury by Mitochondrial Glutaredoxin 2
While the cardiotoxicity of doxorubicin (DOX) is known to be partly mediated through the generation of reactive oxygen species (ROS), the biochemical mechanisms by which ROS damage cardiomyocytes remain to be determined. This study investigates whether S-glutathionylation of mitochondrial proteins plays a role in DOX-induced myocardial injury using a line of transgenic mice expressing the human mitochondrial glutaredoxin 2 (Glrx2), a thiotransferase catalyzing the reduction as well as formation of protein–glutathione mixed disulfides, in cardiomyocytes. The total glutaredoxin (Glrx) activity was increased by 76% and 53 fold in homogenates of whole heart and isolated heart mitochondria of Glrx2 transgenic mice, respectively, compared to those of nontransgenic mice. The expression of other antioxidant enzymes, with the exception of glutaredoxin 1, was unaltered. Overexpression of Glrx2 completely prevents DOX-induced decreases in NAD- and FAD-linked state 3 respiration and respiratory control ratio (RCR) in heart mitochondria at days 1 and 5 of treatment. The extent of DOX-induced decline in left ventricular function and release of creatine kinase into circulation at day 5 of treatment was also greatly attenuated in Glrx2 transgenic mice. Further studies revealed that heart mitochondria overexpressing Glrx2 released less cytochrome c than did controls in response to treatment with tBid or a peptide encompassing the BH3 domain of Bid. Development of tolerance to DOX toxicity in transgenic mice is also associated with an increase in protein S-glutathionylation in heart mitochondria. Taken together, these results imply that S-glutathionylation of heart mitochondrial proteins plays a role in preventing DOX-induced cardiac injury
Effects of vertical distribution of soil inorganic nitrogen on root growth and subsequent nitrogen uptake by field vegetable crops
Information is needed about root growth and N uptake of crops under different soil conditions to increase nitrogen use efficiency in horticultural production. The purpose of this study was to investigate if differences in vertical distribution of soil nitrogen (Ninorg) affected root growth and N uptake of a variety of horticultural crops. Two field experiments were performed each over 2 years with shallow or deep placement of soil Ninorg obtained by management of cover crops. Vegetable crops of leek, potato, Chinese cabbage, beetroot, summer squash and white cabbage reached root depths of 0.5, 0.7, 1.3, 1.9, 1.9 and more than 2.4 m, respectively, at harvest, and showed rates of root depth penetration from 0.2 to 1.5 mm day)1 C)1. Shallow placement of soil Ninorg resulted in greater N uptake in the shallow-rooted leek and potato. Deep placement of soil Ninorg resulted in greater rates of root depth penetration in the deep-rooted Chinese cabbage, summer squash and white cabbage, which increased their depth by 0.2–0.4 m. The root frequency was decreased in shallow soil layers (white cabbage) and increased in deep soil layers (Chinese cabbage, summer squash and white cabbage). The influence of vertical distribution of soil Ninorg on root distribution and capacity for depletion of soil Ninorg was much less than the effect of inherent differences between species. Thus, knowledge about differences in root growth between species should be used when designing crop rotations with high N use efficiency
The influence of direct -meson production to the determination on the nucleon strangeness asymmetry via dimuon events in neutrino experiments
Experimentally, the production of oppositely charged dimuon events by
neutrino and anti-neutrino deep inelastic scattering (DIS) is used to determine
the strangeness asymmetry inside a nucleon. Here we point out that the direct
production of -meson in DIS may make substantial influence to the
measurement of nucleon strange distributions. The direct -meson production
is via the heavy quark recombination (HQR) and via the light quark
fragmentation from perturbative QCD (LQF-P). To see the influence precisely, we
compute the direct -meson productions via HQR and LQF-P quantitatively and
estimate their corrections to the analysis of the strangeness asymmetry. The
results show that HQR has stronger effect than LQF-P does, and the former may
influence the experimental determination of the nucleon strangeness asymmetry.Comment: 9 latex pages, 7 figure
Transient elastohydrodynamic lubrication analysis of a novel metal-on-metal hip prosthesis with a non-spherical femoral bearing surface
Effective lubrication performance of metal-on-metal hip implants only requires optimum conformity within the main loaded area, while it is advantageous to increase the clearance in the equatorial region. Such a varying clearance can be achieved by using non-spherical bearing surfaces for either acetabular or femoral components. An elastohydrodynamic lubrication model of a novel metal-on-metal hip prosthesis using a non-spherical femoral bearing surface against a spherical cup was solved under loading and motion conditions specified by ISO standard. A full numerical methodology of considering the geometric variation in the rotating non-spherical head in elastohydrodynamic lubrication solution was presented, which is applicable to all non-spherical head designs. The lubrication performance of a hip prosthesis using a specific non-spherical femoral head, Alpharabola, was analysed and compared with those of spherical bearing surfaces and a non-spherical Alpharabola cup investigated in previous studies. The sensitivity of the lubrication performance to the anteversion angle of the Alpharabola head was also investigated. Results showed that the non-spherical head introduced a large squeeze-film action and also led to a large variation in clearance within the loaded area. With the same equatorial clearance, the lubrication performance of the metal-on-metal hip prosthesis using an Alpharabola head was better than that of the conventional spherical bearings but worse than that of the metal-on-metal hip prosthesis using an Alpharabola cup. The reduction in the lubrication performance caused by the initial anteversion angle of the non-spherical head was small, compared with the improvement resulted from the non-spherical geometry
Observation of Two New N* Peaks in J/psi -> and Decays
The system in decays of is limited to be
isospin 1/2 by isospin conservation. This provides a big advantage in studying
compared with and experiments which mix
isospin 1/2 and 3/2 for the system. Using 58 million decays
collected with the Beijing Electron Positron Collider, more than 100 thousand
events are obtained. Besides two well known
peaks at 1500 MeV and 1670 MeV, there are two new, clear peaks in
the invariant mass spectrum around 1360 MeV and 2030 MeV. They are the
first direct observation of the peak and a long-sought "missing"
peak above 2 GeV in the invariant mass spectrum. A simple
Breit-Wigner fit gives the mass and width for the peak as MeV and MeV, and for the new peak above 2 GeV
as MeV and MeV, respectively
Treatment of backscattering in a gas of interacting fermions confined to a one-dimensional harmonic atom trap
An asymptotically exact many body theory for spin polarized interacting
fermions in a one-dimensional harmonic atom trap is developed using the
bosonization method and including backward scattering. In contrast to the
Luttinger model, backscattering in the trap generates one-particle potentials
which must be diagonalized simultaneously with the two-body interactions.
Inclusion of backscattering becomes necessary because backscattering is the
dominant interaction process between confined identical one-dimensional
fermions. The bosonization method is applied to the calculation of one-particle
matrix elements at zero temperature. A detailed discussion of the validity of
the results from bosonization is given, including a comparison with direct
numerical diagonalization in fermionic Hilbert space. A model for the
interaction coefficients is developed along the lines of the Luttinger model
with only one coupling constant . With these results, particle densities,
the Wigner function, and the central pair correlation function are calculated
and displayed for large fermion numbers. It is shown how interactions modify
these quantities. The anomalous dimension of the pair correlation function in
the center of the trap is also discussed and found to be in accord with the
Luttinger model.Comment: 19 pages, 5 figures, journal-ref adde
Loop effects and non-decoupling property of SUSY QCD in
One-loop SUSY QCD radiative correction to cross section is
calculated in the Minimal Supersymmetric Standard Model. We found that SUSY QCD
is non-decoupling if the gluino mass and the parameter , or
are at the same order and get large. The non-decoupling contribution can be
enhanced by large and therefore large corrections to the hadronic
production rates at the Tevatron and LHC are expected in the large
limit. The fundamental reason for such non-decoupling behavior is found to be
some couplings in the loops being proportional to SUSY mass parameters.Comment: 15 pages, 5 PS figures. A proof of non-decouplings of SUSY-QCD,
Comments on corresponding QCD correction and references adde
Higgs-boson production associated with a bottom quark at hadron colliders with SUSY-QCD corrections
The Higgs boson production p p (p\bar p) -> b h +X via b g -> b h at the LHC,
which may be an important channel for testing the bottom quark Yukawa coupling,
is subject to large supersymmetric quantum corrections. In this work the
one-loop SUSY-QCD corrections to this process are evaluated and are found to be
quite sizable in some parameter space. We also study the behavior of the
corrections in the limit of heavy SUSY masses and find the remnant effects of
SUSY-QCD. These remnant effects, which are left over in the Higgs sector by the
heavy sparticles, are found to be so sizable (for a light CP-odd Higgs and
large \tan\beta) that they might be observable in the future LHC experiment.
The exploration of such remnant effects is important for probing SUSY,
especially in case that the sparticles are too heavy (above TeV) to be directly
discovered at the LHC.Comment: Results for the Tevatron adde
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