30 research outputs found
Eindämmung des Erregers der Anthraknose der Lupine im ökologischen Landbau durch Anbau weniger anfälliger Lupinensorten der drei landwirtschaftlich wichtigen Lupinenarten
Das Projekt befasste sich mit einem Sortenscreening von 23 Lupinus albus-, 14 L. luteus- und 21 L. angustifolius-Sorten auf die Reaktion gegenüber einer Infektion mit Colletotrichum lupini var. setosum. Für die Infektion der Samen wurde der Substrattest, eine Methode, die die Primärinfektion des Pathogens simuliert, genutzt. Der Einfluß der Infektion auf verschiedene Merkmale, wie Überlebensrate der Pflanzen, Symptome, Befallsverlauf und auf den Ertrag wurden bewertet. Die drei Lupinenarten zeigten unterschiedliche Krankheitssymptome. Während die Symptome bei L. luteus und L.angustifolius oft untypisch waren, entwickelten sich bei L. albus bekanntere Anthraknosesymptome. Unterschiedliche Verhaltensweisen der drei Lupinenarten waren auch im Befallsverlauf zu finden. Die Versuche lieferten interessante Ergebnisse zum Verhalten der einzelnen Lupinenarten und -sorten gegenüber der Infektion mit dem Anthraknoseerreger. Die höchste Absterberate der Pflanzen aller 3 Lupinenarten war während des Keimlings- und Jungpflanzenstadiums. Der Entwicklungsverlauf der Mykose und die Symptomausprägung waren jedoch bei den Lupinenarten unterschiedlich. Die Überlebensrate der Pflanzen war stark abhängig von der jeweiligen Sorte.
Die Ergebnisse sind eine fundierte Materialgrundlage zur Krankheit Anthraknose. Die Sortenunterschiede lassen ein erhebliches Toleranzpotential gegenüber dem Pilz Colletotrichum lupini var. setosum erkennen, so daß entsrechend unterschiedlicher Fragestellungen Sorten anhand der Projektergebnisse auswählt werden können
A revision of Cyanonectria and Geejayessia gen. nov., and related species with Fusarium-like anamorphs
A revision of Fusarium-like species associated with the plant
genus Buxus led to a reconsideration of generic concepts in the
Fusarium clade of the Nectriaceae. Phylogenetic analyses of
the partial second largest subunit of the RNA polymerase II (rpb2)
and the larger subunit of the ATP citrate lyase (acl1) gene exons
confirm the existence of a clade, here called the terminal Fusarium
clade, that includes genera such as Fusarium sensu stricto
(including its Gibberella teleomorphs), Albonectria,
Cyanonectria, “Haematonectria”, the newly
described genus Geejayessia, and “Nectria”
albida. Geejayessia accommodates five species. Four were
previously classified in Nectria sensu lato, namely the black
perithecial, KOH–species G. atrofusca and the orange or
reddish, KOH+ G. cicatricum, G. desmazieri and G.
zealandica. Geejayessia celtidicola is newly described.
Following our phylogenetic analyses showing its close relationship with
Cyanonectria cyanostoma, the former Gibbera buxi is
recombined as the second species of Cyanonectria. A three gene
phylogenetic analysis of multiple strains of each morphological species using
translation elongation factor 1 α (tef-1), rpb2 and
acl1 gene exons and introns confirms their status as distinct
phylogenetic species. Internal transcribed spacer of the ribosomal RNA gene
cluster and nuclear large ribosomal subunit sequences were generated as
additional DNA barcodes for selected strains. The connection of Fusarium
buxicola, often erroneously reported as the anamorph of G.
desmazieri, with the bluish black and KOH+ perithecial species C.
buxi is reinstated. Most Cyanonectria and Geejayessia
species exhibit restricted host ranges on branches or twigs of Buxus
species, Celtis occidentalis, or Staphylea
trifolia. Their perithecia form caespitose clusters on
well-developed, mostly erumpent stromata on the bark or outer cortex of the
host and are relatively thin-walled, mostly smooth, and therefore reminiscent
of the more or less astromatous, singly occurring perithecia of
Cosmospora, Dialonectria, and Microcera. The cell walls in
outer- and inner layers of the perithecial walls of Cyanonectria and
Geejayessia have inconspicuous pore-like structures, as do
representative species of Albonectria, Fusarium sensu stricto,
“Haematonectria”, and “Nectria”
albida. The taxonomic significance of these structures, which we call
Samuels' pores, is discussed
An overview of the taxonomy, phylogeny, and typification of nectriaceous fungi in Cosmospora, Acremonium, Fusarium, Stilbella, and Volutella
A comprehensive phylogenetic reassessment of the ascomycete genus
Cosmospora (Hypocreales, Nectriaceae) is undertaken using
fresh isolates and historical strains, sequences of two protein encoding
genes, the second largest subunit of RNA polymerase II (rpb2), and a
new phylogenetic marker, the larger subunit of ATP citrate lyase
(acl1). The result is an extensive revision of taxonomic concepts,
typification, and nomenclatural details of many anamorph- and
teleomorph-typified genera of the Nectriaceae, most notably
Cosmospora and Fusarium. The combined phylogenetic analysis
shows that the present concept of Fusarium is not monophyletic and
that the genus divides into two large groups, one basal in the family, the
other terminal, separated by a large group of species classified in genera
such as Calonectria, Neonectria, and Volutella. All accepted
genera received high statistical support in the phylogenetic analyses.
Preliminary polythetic morphological descriptions are presented for each
genus, providing details of perithecia, micro- and/or macro-conidial
synanamorphs, cultural characters, and ecological traits. Eight species are
included in our restricted concept of Cosmospora, two of which have
previously documented teleomorphs and all of which have
Acremonium-like microconidial anamorphs. A key is provided to the
three anamorphic species recognised in Atractium, which is removed
from synonymy with Fusarium and epitypified for two macroconidial
synnematous species and one sporodochial species associated with waterlogged
wood. Dialonectria is recognised as distinct from Cosmospora
and two species with teleomorph, macroconidia and microconidia are accepted,
including the new species D. ullevolea. Seven species, one with a
known teleomorph, are classified in Fusicolla, formerly considered a
synonym of Fusarium including members of the F. aquaeductuum
and F. merismoides species complex, with several former varieties
raised to species rank. Originally a section of Nectria,
Macroconia is raised to generic rank for five species, all producing
a teleomorph and macroconidial anamorph. A new species of the
Verticillium-like anamorphic genus Mariannaea is described
as M. samuelsii. Microcera is recognised as distinct from
Fusarium and a key is included for four macroconidial species, that
are usually parasites of scale insects, two of them with teleomorphs. The four
accepted species of Stylonectria each produce a teleomorph and micro-
and macroconidial synanamorphs. The Volutella species sampled fall
into three clades. Pseudonectria is accepted for a perithecial and
sporodochial species that occurs on Buxus. Volutella s. str.
also includes perithecial and/or sporodochial species and is revised to
include a synnematous species formerly included in Stilbella. The
third Volutella-like clade remains unnamed. All fungi in this paper
are named using a single name system that gives priority to the oldest generic
names and species epithets, irrespective of whether they are originally based
on anamorph or teleomorph structures. The rationale behind this is
discussed
Eindämmung des Erregers der Anthraknose der Lupine im ökologischen Landbau durch Anbau weniger anfälliger Lupinensorten der drei landwirtschaftlich wichtigen Lupinenarten
Das Projekt befasste sich mit einem Sortenscreening von 23 Lupinus albus-, 14 L. luteus- und 21 L. angustifolius-Sorten auf die Reaktion gegenüber einer Infektion mit Colletotrichum lupini var. setosum. Für die Infektion der Samen wurde der Substrattest, eine Methode, die die Primärinfektion des Pathogens simuliert, genutzt. Der Einfluß der Infektion auf verschiedene Merkmale, wie Überlebensrate der Pflanzen, Symptome, Befallsverlauf und auf den Ertrag wurden bewertet. Die drei Lupinenarten zeigten unterschiedliche Krankheitssymptome. Während die Symptome bei L. luteus und L.angustifolius oft untypisch waren, entwickelten sich bei L. albus bekanntere Anthraknosesymptome. Unterschiedliche Verhaltensweisen der drei Lupinenarten waren auch im Befallsverlauf zu finden. Die Versuche lieferten interessante Ergebnisse zum Verhalten der einzelnen Lupinenarten und -sorten gegenüber der Infektion mit dem Anthraknoseerreger. Die höchste Absterberate der Pflanzen aller 3 Lupinenarten war während des Keimlings- und Jungpflanzenstadiums. Der Entwicklungsverlauf der Mykose und die Symptomausprägung waren jedoch bei den Lupinenarten unterschiedlich. Die Überlebensrate der Pflanzen war stark abhängig von der jeweiligen Sorte.
Die Ergebnisse sind eine fundierte Materialgrundlage zur Krankheit Anthraknose. Die Sortenunterschiede lassen ein erhebliches Toleranzpotential gegenüber dem Pilz Colletotrichum lupini var. setosum erkennen, so daß entsrechend unterschiedlicher Fragestellungen Sorten anhand der Projektergebnisse auswählt werden können
Dispersal of Pseudocercosporella herpotrichoides and Pyrenopeziza brassicae spores in splash droplets
Splash dispersal of conidia which differ in shape and size, those of Pseudocercosporella herpo ichoides (needle-shaped, 52Ă—2 ÎĽm) and Pyrenopeziza hrassicae (cylindrical, 12Ă—3 ÎĽm), was studisd by allowing 5 mm drops to fall 13 m on to suspensions (depth 0.5 mm, concentration 120, 000 spores/ml) of each. The resulting splash droplets were collected on photographic film. The pattern of spore dispersal with distance and the distribution of spore-carrying droplets and spores within droplet-size categories were similar for both fungi. Regressions of square root spore number) on droplet diameter gave lines with slopes of 0.0138 for P. herpotrichoides and 0.0167 for P. brassicae.Peer reviewe