Sidewall depletion in nano-patterned LAO/STO heterostructures

We report the fabrication of nanostructures from the quasi-two-dimensional electron gas (q2DEG) formed at the LaAlO$_{3}$/ SrTiO$_{3}$ (LAO/STO) interface. The process uses electron beam lithography in combination with reactive ion etching. This technique allows to pattern high-quality structures down to lateral dimensions as small as $100$nm while maintaining the conducting properties without inducing conductivity in the STO substrate. Temperature dependent transport properties of patterned Hall bars of various widths show only a small size dependence of conductivity at low temperature as well as at room temperature. The deviation can be explained by a narrow lateral depletion region. All steps of the patterning process are fully industry compatible.Comment: 5 pages, 4 figure

The Holocene thermal maximum in the Nordic Seas: the impact of Greenland Ice Sheet melt and other forcings in a coupled atmosphere-sea ice-ocean model

The relatively warm early Holocene climate in the Nordic Seas, known as the Holocene Thermal Maximum (HTM), is often associated with an orbitally forced summer insolation maximum at 10 ka BP. The spatial and temporal response recorded in proxy data in the North Atlantic and the Nordic Seas reveal a complex interaction of mechanisms active in the HTM. Previous studies have investigated the impact of the Laurentide Ice Sheet (LIS), as a remnant from a previous glacial period, altering climate conditions with a continuous supply of melt water to the Labrador Sea and adjacent seas and with a downwind cooling effect from the remnant LIS. In our present work we extend this approach by investigating the impact of the Greenland Ice Sheet (GIS) on the early Holocene climate and the HTM. Reconstructions suggest melt rates of 13 mSv for 9 ka BP, which result in our model in a ocean surface cooling of up to 2 K near Greenland. Reconstructed summer SST gradients agree best with our simulation including GIS melt, confirming that the impact of early Holocene GIS is crucial for understanding the HTM characteristics in the Nordic Seas area. This implies that the modern and near-future GIS melt can be expected to play an active role in the climate system in the centuries to come

The impact of early Holocene Arctic Shelf flooding on climate in an atmosphere–ocean–sea–ice model

Glacial terminations are characterized by a strong rise in sea level related to melting ice sheets. This rise in sea level is not uniform all over the world, because regional effects (uplift and subsidence of coastal zones) are superimposed on global trends. During the early Holocene the Siberian Shelf became flooded before 7.5 ka BP and the coastline reached modern-day high stands at 5 ka BP. This area is currently known as a sea-ice production area and contributes significantly to the sea-ice exported from the Arctic through the Fram Strait. This leads to the following hypothesis: during times of rising sea levels, shelves become flooded, increasing sea-ice production on these shelves, increasing sea-ice volume and export through the Fram Strait and causing the sea-ice extent to advance in the Nordic Seas, yielding cooler and fresher sea surface conditions. We have tested this hypothesis in an atmosphere–ocean–sea–ice coupled model of intermediate complexity (LOVECLIM). Our experiment on early Holocene Siberian Shelf flooding shows that in our model sea-ice production in the Northern Hemisphere increases (15%) and that sea-ice extent in the Northern Hemisphere increases (14%) but sea-ice export decreases (−15%) contrary to our hypothesis. The reason of this unexpected behaviour has its origin in a weakened polar vortex, induced by the land–ocean changes due to the shelf flooding, and a resulting decrease of zonality in the Nordic Seas pressure regime. Hence the winter Greenland high and the Icelandic low strengthen, yielding stronger winds on both sides of the Nordic Seas. Increased winds along the East Greenland Current support local sea-ice production and transport towards the South, resulting in a wider sea-ice cover and a southward shift of convection areas. The overall strength of the Atlantic meridional overturning circulation is reduced by 4% and the heat transport in the Atlantic basin by 7%, resulting in an annual cooling pattern over the Nordic Seas by up to −4 °C. We find that the flooding of the Siberian shelf resulting from an orbitally induced warming and related glacioeustatic sea level rise causes a Nordic Seas cooling feedback opposed to this warming

Pretreatment and conversion of distiller's dried grains with solubles for acetone-butanol-ethanol (ABE) production

Distillers dried grains with solubles (DDGS) is a low-value co-product from dry grind ethanol production. Due to its high fiber content, DDGS is used primarily as a feed for ruminant animals. Conversion of the carbohydrate components in DDGS to biofuel would improve the overall economics of dry grind ethanol production. The effect of solids loading oil pretreatment of DDGS and oil subsequent acetone-butanol-ethanol (ABE) fermentation was examined. DDGS samples were subjected to six pretreatments, involving acidic and alkaline electrolyzed water, at three solids loadings (20%, 30%, and 40% w/w). After a 72 h hydrolysis, the highest glucose yield was obtained from the sulfuric acid pretreated samples. In fermentation tests using Clostridium acetobutylicum P260 for ABE production, the highest ABE concentration was similar to 17 g L-1, which was achieved from alkaline electrolyzed water pretreated samples at 30% (w/w) solids loading. The solids loading in a pretreatment significantly affected both the sugar yield from enzymatic hydrolysis and the ABE yield from fermentation of DDGS hydrolysates. The hydrolysate from alkaline electrolyzed water pretreatment at 30% (w/w) solids was found to be most favorable to ABE production, while that from sulfuric acid pretreatment was the least fermentable, although it produced the highest sugar yield

Long-term variations in Iceland–Scotland overflow strength during the Holocene

The overflow of deep water from the Nordic seas into the North Atlantic plays a critical role in global ocean circulation and climate. Approximately half of this overflow occurs via the Iceland–Scotland (I–S) overflow, yet the history of its strength throughout the Holocene (~ 0–11 700 yr ago, ka) is poorly constrained, with previous studies presenting apparently contradictory evidence regarding its long-term variability. Here, we provide a comprehensive reconstruction of I–S overflow strength throughout the Holocene using sediment grain size data from a depth transect of 13 cores from the Iceland Basin. Our data are consistent with the hypothesis that the main axis of the I–S overflow on the Iceland slope was shallower during the early Holocene, deepening to its present depth by ~ 7 ka. Our results also reveal weaker I–S overflow during the early and late Holocene, with maximum overflow strength occurring at ~ 7 ka, the time of a regional climate thermal maximum. Climate model simulations suggest a shoaling of deep convection in the Nordic seas during the early and late Holocene, consistent with our evidence for weaker I–S overflow during these intervals. Whereas the reduction in I–S overflow strength during the early Holocene likely resulted from melting remnant glacial ice sheets, the decline throughout the last 7000 yr was caused by an orbitally induced increase in the amount of Arctic sea ice entering the Nordic seas. Although the flux of Arctic sea ice to the Nordic seas is expected to decrease throughout the next century, model simulations predict that under high emissions scenarios, competing effects, such as warmer sea surface temperatures in the Nordic seas, will result in reduced deep convection, likely driving a weaker I–S overflow

Long-term variations in Iceland–Scotland overflow strength during the Holocene

The overflow of deep water from the Nordic seas into the North Atlantic plays a critical role in global ocean circulation and climate. Approximately half of this overflow occurs via the Iceland–Scotland (I–S) overflow, yet the history of its strength throughout the Holocene (~0–11 700 yr ago, ka) is poorly constrained, with previous studies presenting apparently contradictory evidence regarding its long-term variability. Here, we provide a comprehensive reconstruction of I–S overflow strength throughout the Holocene using sediment grain size data from a depth transect of 13 cores from the Iceland Basin. Our data are consistent with the hypothesis that the main axis of the I–S overflow on the Iceland slope was shallower during the early Holocene, deepening to its present depth by ~7 ka. Our results also reveal weaker I–S overflow during the early and late Holocene, with maximum overflow strength occurring at ~7 ka, the time of a regional climate thermal maximum. Climate model simulations suggest a shoaling of deep convection in the Nordic seas during the early and late Holocene, consistent with our evidence for weaker I–S overflow during these intervals. Whereas the reduction in I–S overflow strength during the early Holocene likely resulted from melting remnant glacial ice sheets, the decline throughout the last 7000 yr was caused by an orbitally induced increase in the amount of Arctic sea ice entering the Nordic seas. Although the flux of Arctic sea ice to the Nordic seas is expected to decrease throughout the next century, model simulations predict that under high emissions scenarios, competing effects, such as warmer sea surface temperatures in the Nordic seas, will result in reduced deep convection, likely driving a weaker I–S overflow

Single-nucleotide resolution analysis of the transcriptome structure of Clostridium beijerinckii NCIMB 8052 using RNA-Seq

<p>Abstract</p> <p>Background</p> <p><it>Clostridium beijerinckii </it>is an important solvent producing microorganism. The genome of <it>C. beijerinckii </it>NCIMB 8052 has recently been sequenced. Although transcriptome structure is important in order to reveal the functional and regulatory architecture of the genome, the physical structure of transcriptome for this strain, such as the operon linkages and transcript boundaries are not well understood.</p> <p>Results</p> <p>In this study, we conducted a single-nucleotide resolution analysis of the <it>C. beijerinckii </it>NCIMB 8052 transcriptome using high-throughput RNA-Seq technology. We identified the transcription start sites and operon structure throughout the genome. We confirmed the structure of important gene operons involved in metabolic pathways for acid and solvent production in <it>C. beijerinckii </it>8052, including <it>pta</it>-<it>ack</it>, <it>ptb</it>-<it>buk</it>, <it>hbd</it>-<it>etfA</it>-<it>etfB</it>-<it>crt </it>(<it>bcs</it>) and <it>ald</it>-<it>ctfA</it>-<it>ctfB</it>-<it>adc </it>(<it>sol</it>) operons; we also defined important operons related to chemotaxis/motility, transcriptional regulation, stress response and fatty acids biosynthesis along with others. We discovered 20 previously non-annotated regions with significant transcriptional activities and 15 genes whose translation start codons were likely mis-annotated. As a consequence, the accuracy of existing genome annotation was significantly enhanced. Furthermore, we identified 78 putative silent genes and 177 putative housekeeping genes based on normalized transcription measurement with the sequence data. We also observed that more than 30% of pseudogenes had significant transcriptional activities during the fermentation process. Strong correlations exist between the expression values derived from RNA-Seq analysis and microarray data or qRT-PCR results.</p> <p>Conclusions</p> <p>Transcriptome structural profiling in this research provided important supplemental information on the accuracy of genome annotation, and revealed additional gene functions and regulation in <it>C. beijerinckii</it>.</p

Sea-ice dynamics in an Arctic coastal polynya during the past 6500 years

The production of high-salinity brines during sea-ice freezing in circum-arctic coastal polynyas is thought to be part of northern deep water formation as it supplies additional dense waters to the Atlantic meridional overturning circulation system. To better predict the effect of possible future summer ice-free conditions in the Arctic Ocean on global climate, it is important to improve our understanding of how climate change has affected sea-ice and brine formation, and thus finally dense water formation during the past. Here, we show temporal coherence between sea-ice conditions in a key Arctic polynya (Storfjorden, Svalbard) and patterns of deep water convection in the neighbouring Nordic Seas over the last 6500 years. A period of frequent sea-ice melting and freezing between 6.5 and 2.8 ka BP coincided with enhanced deep water renewal in the Nordic Seas. Near-permanent sea-ice cover and low brine rejection after 2.8 ka BP likely reduced the overflow of high-salinity shelf waters, concomitant with a gradual slow down of deep water convection in the Nordic Seas, which occurred along with a regional expansion in sea-ice and surface water freshening. The Storfjorden polynya sea-ice factory restarted at ~0.5 ka BP, coincident with renewed deep water penetration to the Arctic and climate amelioration over Svalbard. The identified synergy between Arctic polynya sea-ice conditions and deep water convection during the present interglacial is an indication of the potential consequences for ocean ventilation during states with permanent sea-ice cover or future Arctic ice-free conditions

Monogenic variants in dystonia: an exome-wide sequencing study

Background Dystonia is a clinically and genetically heterogeneous condition that occurs in isolation (isolated dystonia), in combination with other movement disorders (combined dystonia), or in the context of multisymptomatic phenotypes (isolated or combined dystonia with other neurological involvement). However, our understanding of its aetiology is still incomplete. We aimed to elucidate the monogenic causes for the major clinical categories of dystonia. Methods For this exome-wide sequencing study, study participants were identified at 33 movement-disorder and neuropaediatric specialty centres in Austria, Czech Republic, France, Germany, Poland, Slovakia, and Switzerland. Each individual with dystonia was diagnosed in accordance with the dystonia consensus definition. Index cases were eligible for this study if they had no previous genetic diagnosis and no indication of an acquired cause of their illness. The second criterion was not applied to a subset of participants with a working clinical diagnosis of dystonic cerebral palsy. Genomic DNA was extracted from blood of participants and whole-exome sequenced. To find causative variants in known disorder-associated genes, all variants were filtered, and unreported variants were classified according to American College of Medical Genetics and Genomics guidelines. All considered variants were reviewed in expert round-table sessions to validate their clinical significance. Variants that survived filtering and interpretation procedures were defined as diagnostic variants. In the cases that went undiagnosed, candidate dystonia-causing genes were prioritised in a stepwise workflow. Findings We sequenced the exomes of 764 individuals with dystonia and 346 healthy parents who were recruited between June 1, 2015, and July 31, 2019. We identified causative or probable causative variants in 135 (19%) of 728 families, involving 78 distinct monogenic disorders. We observed a larger proportion of individuals with diagnostic variants in those with dystonia (either isolated or combined) with coexisting non-movement disorder-related neurological symptoms (100 [45%] of 222;excepting cases with evidence of perinatal brain injury) than in those with combined (19 [19%] of 98) or isolated (16 [4%] of 388) dystonia. Across all categories of dystonia, 104 (65%) of the 160 detected variants affected genes which are associated with neurodevelopmental disorders. We found diagnostic variants in 11 genes not previously linked to dystonia, and propose a predictive clinical score that could guide the implementation of exome sequencing in routine diagnostics. In cases without perinatal sentinel events, genomic alterations contributed substantively to the diagnosis of dystonic cerebral palsy. In 15 families, we delineated 12 candidate genes. These include IMPDH2, encoding a key purine biosynthetic enzyme, for which robust evidence existed for its involvement in a neurodevelopmental disorder with dystonia. We identified six variants in IMPDH2, collected from four independent cohorts, that were predicted to be deleterious de-novo variants and expected to result in deregulation of purine metabolism. Interpretation In this study, we have determined the role of monogenic variants across the range of dystonic disorders, providing guidance for the introduction of personalised care strategies and fostering follow-up pathophysiological explorations