Accurate interlaminar stress recovery from finite element analysis

The accuracy and robustness of a two-dimensional smoothing methodology is examined for the problem of recovering accurate interlaminar shear stress distributions in laminated composite and sandwich plates. The smoothing methodology is based on a variational formulation which combines discrete least-squares and penalty-constraint functionals in a single variational form. The smoothing analysis utilizes optimal strains computed at discrete locations in a finite element analysis. These discrete strain data are smoothed with a smoothing element discretization, producing superior accuracy strains and their first gradients. The approach enables the resulting smooth strain field to be practically C1-continuous throughout the domain of smoothing, exhibiting superconvergent properties of the smoothed quantity. The continuous strain gradients are also obtained directly from the solution. The recovered strain gradients are subsequently employed in the integration o equilibrium equations to obtain accurate interlaminar shear stresses. The problem is a simply-supported rectangular plate under a doubly sinusoidal load. The problem has an exact analytic solution which serves as a measure of goodness of the recovered interlaminar shear stresses. The method has the versatility of being applicable to the analysis of rather general and complex structures built of distinct components and materials, such as found in aircraft design. For these types of structures, the smoothing is achieved with 'patches', each patch covering the domain in which the smoothed quantity is physically continuous

Hydro- and aero-elastic response of floating offshore wind turbines to combined waves and wind in frequency domain

An analytical approach and numerical solution to determine coupled aeroelastic and hydroelastic response of floating offshore wind turbines of arbitrary shape to combined wind and wave loads is presented. The model considers simultaneously the aerodynamic and hydrodynamic loads on an FOWT and integrates these with finite element method for structural analysisdue to the combined loads. The hydrodynamic and aerodynamic loads are determined based on the linear wave diffraction theory and steady blade element momentum method, respectively, and the solution is obtained in frequency domain. The structure may be fixed or floating, located in arbitrary water depth, and may host single or multiple wind towers. The model captures the complete translational and rotational motions of the body in three dimensions, and the elasticity of the blades, tower and the floating platform. To assess the performance of the model, rigid and elastic responses of a FOWT to combined wave and wind loads are computed and compared with available laboratory measurements and other theoretical approacheswhere possible, and overall very good agreement is observed. The model developed in this study addresses directly three shortcomings of existing approaches used for the analysis of FOWTs, namely (i) determination of the elastic responses of the entire structure including the floating platform, (ii) analysis of the motion and elastic response of FOWTs in frequency domain, and (iii) assessment of responses of FOWTs with single or multiple wind towers

Dynamic response of multi-unit floating offshore wind turbines to wave, current and wind loads

Motion of a multi-unit wind-tracing floating offshore wind turbine (FOWT) to combined wave–current and wind is obtained in the frequency-domain. The linear diffraction wave theory with a Green function for small current speeds and the blade-element momentum method are used for the hydrodynamic and aerodynamic analysis, respectively. A finite-element method is coupled with the hydrodynamic and aerodynamic equations to obtain the elastic responses of the FOWT to the environmental loads. The wind-tracing FOWT consists of three 5 MW wind turbines installed at the corners of an equilateral triangular platform. The platform is connected to the seabed through a turret-bearing mooring system, allowing the structure to rotate and face the dominant wind direction; hence, the multi-unit FOWT is called the wind-tracing FOWT. In this study, rigid-body responses of the wind-tracing FOWT to waves and wind are compared with those to combined wave, current, and wind loads for several current speeds and various wave heading angles. For a chosen current speed and wave heading angle, hydro- and aeroelastic responses of the wind-tracing FOWT to combined waves, current, and wind are obtained and compared with those of the rigid structure. Discussion is provided on the effect of the wave–current interaction on the motion and elastic responses of the wind-tracing FOWT. The numerical results show that under the rated wind speed, the motion of the wind-tracing FOWT is mainly governed by the wave-induced hydrodynamic forces and moments and the presence of current results in larger elastic motion of the FOWT to the environmental loads

Dynamic response of multi-unit floating offshore wind turbines to wave, current and wind loads

Motion of a multi-unit wind-tracing floating offshore wind turbine (FOWT) to combined wave–current and wind is obtained in the frequency-domain. The linear diffraction wave theory with a Green function for small current speeds and the blade-element momentum method are used for the hydrodynamic and aerodynamic analysis, respectively. A finite-element method is coupled with the hydrodynamic and aerodynamic equations to obtain the elastic responses of the FOWT to the environmental loads. The wind-tracing FOWT consists of three 5 MW wind turbines installed at the corners of an equilateral triangular platform. The platform is connected to the seabed through a turret-bearing mooring system, allowing the structure to rotate and face the dominant wind direction; hence, the multi-unit FOWT is called the wind-tracing FOWT. In this study, rigid-body responses of the wind-tracing FOWT to waves and wind are compared with those to combined wave, current, and wind loads for several current speeds and various wave heading angles. For a chosen current speed and wave heading angle, hydro- and aeroelastic responses of the wind-tracing FOWT to combined waves, current, and wind are obtained and compared with those of the rigid structure. Discussion is provided on the effect of the wave–current interaction on the motion and elastic responses of the wind-tracing FOWT. The numerical results show that under the rated wind speed, the motion of the wind-tracing FOWT is mainly governed by the wave-induced hydrodynamic forces and moments and the presence of current results in larger elastic motion of the FOWT to the environmental loads

Two Allelic Variants of Aldo-Keto Reductase 1A1 Exhibit Reduced in Vitro Metabolism of Daunorubicin

ABSTRACT: Aldo-keto reductases (AKRs) are a class of NADPH-dependent oxidoreductases that have been linked to metabolism of the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Although widely used, cardiotoxicity continues to be a serious side effect that may be linked to metabolites or reactive intermediates generated in their metabolism. In this study we examine the little known effects of nonsynonymous single nucleotide polymorphisms of human AKR1A1 on the metabolism of these drugs to their alcohol metabolites. Expressed and purified from bacteria using affinity chromatography, the AKR1A1 protein with a single histidine (6x-His) tag exhibited the greatest activity using two test substrates: p-nitrobenzaldehyde (5.09 ؎ 0.16 mol/min/mg of purified protein) and DL-glyceraldehyde (1.24 ؎ 0.17 mol/min/mg). These activities are in agreement with published literature values of nontagged human AKR1A1. The 6x-His-tagged AKR1A1 wild type and allelic variants, E55D and N52S, were subsequently examined for metabolic activity using DAUN and DOX. The tagged variants showed significantly reduced activities (1.10 ؎ 0.42 and 0.72 ؎ 0.47 nmol of daunorubicinol (DAUNol) formed/min/mg of purified protein for E55D and N52S, respectively) compared with the wild type (2.34 ؎ 0.71 nmol/min/mg). The wild type and E55D variant metabolized DOX to doxorubicinol (DOXol); however, the levels fell below the limit of quantitation (25 nM). The N52S variant yielded no detectable DOXol. A kinetic analysis of the DAUN reductase activities revealed that both amino acid substitutions lead to reduced substrate affinity, measured as significant increases in the measured K m for the reduction reaction by AKR1A1. Hence, it is possible that these allelic variants can act as genetic biomarkers for the clinical development of DAUN-induced cardiotoxicity

The Human Phenotype Ontology project:linking molecular biology and disease through phenotype data

The Human Phenotype Ontology (HPO) project, available at http://www.human-phenotype-ontology.org, provides a structured, comprehensive and well-defined set of 10,088 classes (terms) describing human phenotypic abnormalities and 13,326 subclass relations between the HPO classes. In addition we have developed logical definitions for 46% of all HPO classes using terms from ontologies for anatomy, cell types, function, embryology, pathology and other domains. This allows interoperability with several resources, especially those containing phenotype information on model organisms such as mouse and zebrafish. Here we describe the updated HPO database, which provides annotations of 7,278 human hereditary syndromes listed in OMIM, Orphanet and DECIPHER to classes of the HPO. Various meta-attributes such as frequency, references and negations are associated with each annotation. Several large-scale projects worldwide utilize the HPO for describing phenotype information in their datasets. We have therefore generated equivalence mappings to other phenotype vocabularies such as LDDB, Orphanet, MedDRA, UMLS and phenoDB, allowing integration of existing datasets and interoperability with multiple biomedical resources. We have created various ways to access the HPO database content using flat files, a MySQL database, and Web-based tools. All data and documentation on the HPO project can be found online

The James Webb Space Telescope Mission

Twenty-six years ago a small committee report, building on earlier studies, expounded a compelling and poetic vision for the future of astronomy, calling for an infrared-optimized space telescope with an aperture of at least $4m$. With the support of their governments in the US, Europe, and Canada, 20,000 people realized that vision as the $6.5m$ James Webb Space Telescope. A generation of astronomers will celebrate their accomplishments for the life of the mission, potentially as long as 20 years, and beyond. This report and the scientific discoveries that follow are extended thank-you notes to the 20,000 team members. The telescope is working perfectly, with much better image quality than expected. In this and accompanying papers, we give a brief history, describe the observatory, outline its objectives and current observing program, and discuss the inventions and people who made it possible. We cite detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space Telescope Overview, 29 pages, 4 figure