28,696 research outputs found
Infrared detectors - Special interest bibliography with abstracts
Bibliography and abstracts of literature related to infrared detectors used in geoscience researc
Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones
LuxR-type transcriptional regulators play key roles in quorum-sensing systems that employ acyl-homoserine lactones (acyl-HSLs) as signal molecules. These proteins mediate quorum control by changing their interactions with RNA polymerase and DNA in response to binding their cognate acyl-HSL. The evolutionarily related LuxR-type proteins exhibit considerable diversity in primary sequence and in their response to acyl-HSLs having acyl groups of differing length and composition. Little is known about which residues determine acyl-HSL specificity, and less about the evolutionary time scales required to forge new ones. To begin to examine such issues, we have focused on the LuxR protein from Vibrio fischeri, which activates gene transcription in response to binding its cognate quorum signal, 3-oxohexanoyl-homoserine lactone (3OC6HSL). Libraries of luxR mutants were screened for variants exhibiting increased gene activation in response to octanoyl-HSL (C8HSL), with which wild-type LuxR interacts only weakly. Eight LuxR variants were identified that showed a 100-fold increase in sensitivity to C8HSL; these variants also displayed increased sensitivities to pentanoyl-HSL and tetradecanoyl-HSL, while maintaining a wild-type or greater response to 3OC6HSL. The most sensitive variants activated gene transcription as strongly with C8HSL as the wild type did with 3OC6HSL. With one exception, the amino acid residues involved were restricted to the N-terminal, 'signal-binding' domain of LuxR. These residue positions differed from critical positions previously identified via 'loss-of-function' mutagenesis. We have demonstrated that acyl-HSL-dependent quorum-sensing systems can evolve rapidly to respond to new acyl-HSLs, suggesting that there may be an evolutionary advantage to maintaining such plasticity
Noisy Optimization: Convergence with a Fixed Number of Resamplings
It is known that evolution strategies in continuous domains might not
converge in the presence of noise. It is also known that, under mild
assumptions, and using an increasing number of resamplings, one can mitigate
the effect of additive noise and recover convergence. We show new sufficient
conditions for the convergence of an evolutionary algorithm with constant
number of resamplings; in particular, we get fast rates (log-linear
convergence) provided that the variance decreases around the optimum slightly
faster than in the so-called multiplicative noise model. Keywords: Noisy
optimization, evolutionary algorithm, theory.Comment: EvoStar (2014
Selective decay by Casimir dissipation in fluids
The problem of parameterizing the interactions of larger scales and smaller
scales in fluid flows is addressed by considering a property of two-dimensional
incompressible turbulence. The property we consider is selective decay, in
which a Casimir of the ideal formulation (enstrophy in 2D flows, helicity in 3D
flows) decays in time, while the energy stays essentially constant. This paper
introduces a mechanism that produces selective decay by enforcing Casimir
dissipation in fluid dynamics. This mechanism turns out to be related in
certain cases to the numerical method of anticipated vorticity discussed in
\cite{SaBa1981,SaBa1985}. Several examples are given and a general theory of
selective decay is developed that uses the Lie-Poisson structure of the ideal
theory. A scale-selection operator allows the resulting modifications of the
fluid motion equations to be interpreted in several examples as parameterizing
the nonlinear, dynamical interactions between disparate scales. The type of
modified fluid equation systems derived here may be useful in modelling
turbulent geophysical flows where it is computationally prohibitive to rely on
the slower, indirect effects of a realistic viscosity, such as in large-scale,
coherent, oceanic flows interacting with much smaller eddies
Measurement of temperature profiles in hot gases and flames
Computer program was written for calculation of molecular radiative transfer from hot gases. Shape of temperature profile was approximated in terms of simple geometric forms so profile could be characterized in terms of few parameters. Parameters were adjusted in calculations using appropriate radiative-transfer expression until best fit was obtained with observed spectra
Integrability of one degree of freedom symplectic maps with polar singularities
In this paper, we treat symplectic difference equations with one degree of
freedom. For such cases, we resolve the relation between that the dynamics on
the two dimensional phase space is reduced to on one dimensional level sets by
a conserved quantity and that the dynamics is integrable, under some
assumptions. The process which we introduce is related to interval exchange
transformations.Comment: 10 pages, 2 figure
Modified Laplace transformation method at finite temperature: application to infra-red problems of N component theory
Modified Laplace transformation method is applied to N component
theory and the finite temperature problem in the massless limit is re-examined
in the large N limit. We perform perturbation expansion of the dressed thermal
mass in the massive case to several orders and try the massless approximation
with the help of modified Laplace transformation. The contribution with
fractional power of the coupling constant is recovered from the truncated
massive series. The use of inverse Laplace transformation with respect to the
mass square is crucial in evaluating the coefficients of fractional power
terms.Comment: 16pages, Latex, typographical errors are correcte
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