Application of Ecotoxicogenomics for Studying Endocrine Disruption in Vertebrates and Invertebrates

Chemicals released into the environment potentially disrupt the endocrine system in wild animals and humans. Developing organisms are particularly sensitive to estrogenic chemicals. Exposure to estrogens or estrogenic chemicals during critical periods of development induces persistent changes in both reproductive and nonreproductive organs, including persistent molecular alterations. Estrogen-responsive genes and critical developmental windows of various animal species, therefore, need to be identified for investigators to understand the molecular basis of estrogenic activity during embryonic development. For investigators to understand molecular mechanisms of toxicity in various species, toxicogenomics/ecotoxicogenomics, defined as the integration of genomics (transcriptomics, proteomics, metabolomics) into toxicology and ecotoxicology, need to be established as powerful tools for research. As the initial step toward using genomics to examine endocrine-disrupting chemicals, estrogen receptors and other steroid hormone receptors have been cloned in various species, including reptiles, amphibians, and fish, and alterations in the expression of these genes in response to chemicals were investigated. We are identifying estrogen-responsive genes in mouse reproductive tracts using cDNA microarrays and trying to establish microarray systems in the American alligator, roach, medaka, and water fleas (Daphnia magna). It is too early to define common estrogen-responsive genes in various animal species; however, toxicogenomics and ectotoxicogenomics provide powerful tools to help us understand the molecular mechanism of chemical toxicities in various animal species

Photogenerated Carriers in SrTiO3 Probed by Mid-Infrared Absorption

Infrared absorption spectra of SrTiO$_3$ have been measured under above-band-gap photoexcitations to study the properties of photogenerated carriers, which should play important roles in previously reported photoinduced phenomena in SrTiO$_3$. A broad absorption band appears over the entire mid-infrared region under photoexcitation. Detailed energy, temperature, and excitation power dependences of the photoinduced absorption are reported. This photo-induced absorption is attributed to the intragap excitations of the photogenerated carriers. The data show the existence of a high density of in-gap states for the photocarriers, which extends over a wide energy range starting from the conduction and valence band edges.Comment: 5 pages, 5 figures, submitted to J. Phys. Soc. Jp

Mitochondrial and apoptotic neuronal death signaling pathways in cerebral ischemia

AbstractMitochondria play important roles as the powerhouse of the cell. After cerebral ischemia, mitochondria overproduce reactive oxygen species (ROS), which have been thoroughly studied with the use of superoxide dismutase transgenic or knockout animals. ROS directly damage lipids, proteins, and nucleic acids in the cell. Moreover, ROS activate various molecular signaling pathways. Apoptosis-related signals return to mitochondria, then mitochondria induce cell death through the release of pro-apoptotic proteins such as cytochrome c or apoptosis-inducing factor. Although the mechanisms of cell death after cerebral ischemia remain unclear, mitochondria obviously play a role by activating signaling pathways through ROS production and by regulating mitochondria-dependent apoptosis pathways

Constraints on Cardassian Scenario from the Expansion Turnaround Redshift and the Sunyaev-Zeldovich/X-ray Data

Cosmic acceleration is one of the most remarkable cosmological findings of recent years. Although a dark energy component has usually been invoked as the mechanism for the acceleration, A modification of Friedmann equation from various higher dimensional models provides a feasible alternative. Cardassian expansion is one of these scenarios. In this work, we first consider the constraints on the parameter space from the turnaround redshift, z_{q=0}, at which the universe switches from deceleration to acceleration. We show that, for every Omega_m, there exist a unique n_{peak} (Omega_m), which makes z_{q=0} reach its maximum value, [z_{q=0}]_{max} = exp[1/ (2-3n_{peak})] -1, which is unlinearly inverse to Omega_m. If the acceleration happans earlier than z_{q=0} = 0.6, suggested by Type Ia supernovae measurements, we have Omega_m < 0.328 no matter what the power index is, and moreover, for reasonable matter density, Omega_m ~ 0.3, it is found n ~ (-0.45,0.25). We next test this scenario using the Sunyaev-Zeldovich/X-ray data of a sample of 18 galaxy clusters with 0.14 < z < 0.83 compiled by Reese et al. (2002). The constraints from the current SZ/X-ray data is weak, though a model with lower matter density is prefered. A certain range of the model parameters is also consistent with the data.Comment: 18 pages, 4 figures, accepted for publication in Ap

Ultra-stable performance of an underground-based laser interferometer observatory for gravitational waves

In order to detect the rare astrophysical events that generate gravitational wave (GW) radiation, sufficient stability is required for GW antennas to allow long-term observation. In practice, seismic excitation is one of the most common disturbances effecting stable operation of suspended-mirror laser interferometers. A straightforward means to allow more stable operation is therefore to locate the antenna, the ``observatory'', at a ``quiet'' site. A laser interferometer gravitational wave antenna with a baseline length of 20m (LISM) was developed at a site 1000m underground, near Kamioka, Japan. This project was a unique demonstration of a prototype laser interferometer for gravitational wave observation located underground. The extremely stable environment is the prime motivation for going underground. In this paper, the demonstrated ultra-stable operation of the interferometer and a well-maintained antenna sensitivity are reported.Comment: 8 pages, to appear on PR

Sexual reprogramming and estrogenic sensitization in wild fish exposed to ethinylestradiol.

addresses: School of Biosciences, University of Exeter, Exeter EX4 4PS, United Kingdom. [email protected]: Journal Article; Research Support, Non-U.S. Gov'tCopyright © 2009 American Chemical Society. Post print version of article deposited. The final published version is available from: http://dx.doi.org/10.1021/es802661pGlobally, feminization responses in wild male freshwater fish are caused by exposure to estrogenic chemicals, including natural and synthetic estrogens, contained in effluentsfromwastewater treatment works. In U.K. rivers, feminization responses, including intersex, are widespread in wild roach (Rutilus rutilus) populations, and severely affected fish have a reduced reproductive success. We exposed roach to environmentally relevant concentrations of the contraceptive estrogen 17alpha-ethinylestradiol (EE2) for up to 2 years, including intermittent and repeated exposures,to determine effects on sexual development and subsequent responsiveness to estrogen. Exposure of roach to EE2 (at 4 ng/L) for 2 years resulted in sex reversal in males, leading to an all-female population with two cohorts in terms of their stages of ovarian development one paralleling the control females and one at a significantly less advanced stage, which we propose were sex-reversed males. Differing developmental and maturing rates of the putative sex-reversed males compared with control females would question their functional capability as females in the wild. Early-life exposure to environmentally relevant concentrations of EE2 sensitized females to estrogen, as determined by the measurement of the responses of estrogen-sensitive genes in a further EE2 challenge 398 days after the original exposure. In the wild, exposure to environmentally relevant concentrations of EE2 during early life has significantly wider implications for the sexual physiology in fish than has thus far been determined

Functional distinctions associated with the diversity of sex steroid hormone receptors ESR and AR

This is the author accepted manuscript. The final version is availablefrom Elsevier via the DOI in this recordSex steroid hormones including estrogens and androgens play fundamental roles in regulating reproductive activities and they act through estrogen and androgen receptors (ESR and AR). These steroid receptors have evolved from a common ancestor in association with several gene duplications. In most vertebrates, this has resulted in two ESR subtypes (ESR1 and ESR2) and one AR, whereas in teleost fish there are at least three ESRs (ESR1, ESR2a and ESR2b) and two ARs (ARα and ARβ) due to a lineage-specific whole genome duplication. Functional distinctions have been suggested among these receptors, but to date their roles have only been characterized in a limited number of species. Sexual differentiation and the development of reproductive organs are indispensable for all animal species and in vertebrates these events depend on the action of sex steroid hormones. Here we review the recent progress in understanding of the functions of the ESRs and ARs in the development and expression of sexually dimorphic characteristics associated with steroid hormone signaling in vertebrates, with representative fish, amphibians, reptiles, birds and mammals.Japan Society for the Promotion of ScienceMinistry of the Environment, JapanDepartment for Environment, Food and Rural Affairs (Defra), UKNational Institute for Basic BiologyKyushu Universit

No association between the sigma receptor type 1 gene and schizophrenia: results of analysis and meta-analysis of case-control studies

BACKGROUND: Several lines of evidence have supported possible roles of the sigma receptors in the etiology of schizophrenia and mechanisms of antipsychotic efficacy. An association study provided genetic evidence that the sigma receptor type 1 gene (SIGMAR1) was a possible susceptibility factor for schizophrenia, however, it was not replicated by a subsequent study. It is necessary to evaluate further the possibility that the SIGMAR1 gene is associated with susceptibility to schizophrenia. METHODS: A case-control association study between two polymorphisms of the SIGMAR1 gene, G-241T/C-240T and Gln2Pro, and schizophrenia in Japanese population, and meta-analysis including present and previous studies. RESULTS: There was no significant association of any allele or genotype of the polymorphisms with schizophrenia. Neither significant association was observed with hebephrenic or paranoid subtype of schizophrenia. Furthermore, a meta-analysis including the present and previous studies comprising 779 controls and 636 schizophrenics also revealed no significant association between the SIGMAR1 gene and schizophrenia. CONCLUSION: In view of this evidence, it is likely that the SIGMAR1 gene does not confer susceptibility to schizophrenia

Measurement and comparison of individual external doses of high-school students living in Japan, France, Poland and Belarus -- the "D-shuttle" project --

Twelve high schools in Japan (of which six are in Fukushima Prefecture), four in France, eight in Poland and two in Belarus cooperated in the measurement and comparison of individual external doses in 2014. In total 216 high-school students and teachers participated in the study. Each participant wore an electronic personal dosimeter "D-shuttle" for two weeks, and kept a journal of his/her whereabouts and activities. The distributions of annual external doses estimated for each region overlap with each other, demonstrating that the personal external individual doses in locations where residence is currently allowed in Fukushima Prefecture and in Belarus are well within the range of estimated annual doses due to the background radiation level of other regions/countries