Endophytic Mycoflora of Indian Medicinal Plant, Terminalia arjuna and their Biological Activities

Studies were undertaken to isolate, identify and evaluate the biological activities of endophytic mycoflora of Indian medicinal plant, Terminalia arjuna. A total of 20 isolates of endophytic fungi were obtained from the leaves, twigs and bark tissues of the Terminalia arjuna. Out of 20, six isolates exhibited promising antibacterial, antifungal and anti-inflammatory activities when cultivated at shake flask level. The selected isolates were identified on the basis of morphology and ITS gene sequencing. Three isolates, designated as TA BA 1, TA L1 and TA L2 were identified as Aspergillus flavus whereas; the remaining three endophytic fungi were identified as Diaporthe arengae (TA TW2), Alternaria Sp. (TA TW1) and Lasiodiplodia theobromae (TA BA2). Aspergillus flavus was found as the predominant endophyte in leaves and bark tissues of the plant. The crude extract of the test isolates showed considerable antimicrobial activity against common human bacterial (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella abony and Bacillus subtilis) and fungal (Candida albicans, Aspergillus niger and Penicilium sp.) pathogens. The extract of Diaporthe arengae (TA TW2) significantly reduced the concentration of DPPH free radical as percent DPPH scavenging activity was found to be highest (69.56%) in comparison with other isolates. The % inhibition of hemolysis of RBCs was found to be highest (82.85%) with Diaporthe arengae (TA TW2) in comparison (83.26%) with standard drug (Ibuprofen). Among all, the extract of the Diaporthe arengae (TA TW2) showed excellent biological activities and hence was subjected to further characterization. The phytochemical investigation of the extract revealed the presence of terpenoids as the major phytoconstituents which was supported by TLC and UV spectroscopic studies. The results indicate that the isolated endophytes could be the valuable source of these bioactive molecules with diverse biological activities. The bioactivities may be attributed to the terpenoids present in the endophytic extract

Variable - temperature scanning optical and force microscope

The implementation of a scanning microscope capable of working in confocal, atomic force and apertureless near field configurations is presented. The microscope is designed to operate in the temperature range 4 - 300 K, using conventional helium flow cryostats. In AFM mode, the distance between the sample and an etched tungsten tip is controlled by a self - sensing piezoelectric tuning fork. The vertical position of both the AFM head and microscope objective can be accurately controlled using piezoelectric coarse approach motors. The scanning is performed using a compact XYZ stage, while the AFM and optical head are kept fixed, allowing scanning probe and optical measurements to be acquired simultaneously and in concert. The free optical axis of the microscope enables both reflection and transmission experiments to be performed.Comment: 24 pages, 9 figures, submitted to the journal "Review of Scientific Instruments

Perturbations in a Bouncing Brane Model

The question of how perturbations evolve through a bounce in the Cyclic and Ekpyrotic models of the Universe is still a matter of ongoing debate. In this report we show that the collision between boundary branes is in most cases singular even in the full 5-D formalism, and that first order perturbation theory breaks down for at least one perturbation variable. Only in the case that the boundary branes approach each other with constant velocity shortly before the bounce, can a consistent, non singular solution be found. It is then possible to follow the perturbations explicitly until the actual collision. In this case, we find that if a scale invariant spectrum developed on the hidden brane, it will get transferred to the visible brane during the bounce.Comment: 15 pages, minor modifications, a few typos correcte

A review on mouth dissolving tablet

The demand for MDT (Mouth Disintegrating Tablet) has been increasing from the last decade particularly in geriatric, pediatric and patient with some sort of disabilities in swallowing. MDTs are those tablets which when placed in mouth get dissolved rapidly in saliva without the need of liquid and can be swallowed. European pharmacopoeia adopted the term Orodispersible tablet for MDTs. Mouth disintegrating tablets are also known as Fast melting tablets, Orodispersible tablets, fast dissolving/dispersing tablets or melt in mouth tablets. This article reviews the potential benefits offered by MDTs as an oral drug delivery system for various kinds of patients suffering from different diseases and disabilities. Desired characteristics and challenges for developing fast disintegrating drug delivery systems, quality control tests, various techniques used in the preparation of fast disintegrating drug delivery systems like lyophilization technologies, tablet molding method, sublimation techniques, spray drying techniques, mass extrusion technology, direct compression method and uses of super-disintegrates. It also reviews the patented technologies for fast dissolving tablets, advantages and disadvantages of different technologies for preparing fast disintegrating dosage form, future prospective for MDTs. The growing importance for MDTs is due to the potential advantages offered by this technology. MDT is a New Drug Delivery system with least disintegration time and ease of self administratio

Studies on Stability of Processing-type Genotypes of Tomato (Solanum lycopersicum L.)

To study stability of genotypes under three diverse environments, ten genotypes along with two checks of processingtype tomato were evaluated in Randomized Block Design (RBD) with three replications. Environment included three seasons, viz., kharif (2007), rabi (2007-08) and summer (2008) to identify the most stable varieties. Overall performance of PTR-1, PTR-4, PTR-6 and 'Arka Ashish' was found stable for yield per plant, number of branches per plant, % fruit set, % acidity and lycopene content. PTR-4 and PTR-6 were stable for high yield and for good processing traits

Wall influence on dynamics of a microbubble

The nonlinear dynamic behaviour of microscopic bubbles near a wall is investigated. The Keller-Miksis-Parlitz equation is adopted, but modified to account for the presence of the wall. This base model describes the time evolution of the bubble surface, which is assumed to remain spherical, and accounts for the effect of acoustic radiation losses owing to liquid compressibility in the momentum conservation. Two situations are considered: the base case of an isolated bubble in an unbounded medium; and a bubble near a solid wall. In the latter case, the wall influence is modeled by including a symmetrically oscillating image bubble. The bubble dynamics is traced using a numerical solution of the model equation. Subsequently, Floquet theory is used to accurately detect the bifurcation point where bubble oscillations stop following the driving ultrasound frequency and undergo period-changing bifurcations. Of particular interest is the detection of the subcritical period tripling and quadrupling transition. The parametric bifurcation maps are obtained as functions of non-dimensional parameters representing the bubble radius, the frequency and pressure amplitude of the driving ultrasound field and the distance from the wall. It is shown that the presence of the wall generally stabilises the bubble dynamics, so that much larger values of the pressure amplitude are needed to generate nonlinear responses.Comment: 25 pages, 14 figure

Role of magnetic resonance imaging in distinguishing fungal from nonfungal multiple brain abscesses

AbstractCladophialophora bantiana is a neurotropic dematiaceous fungus known for affecting immunocompromised and immunocompetent hosts. We report a case of 24year old immunocompetent male presenting with headache, fever and vomiting. MRI was suggestive of multiple fungal brain abscesses. He underwent total excision of abscesses. Pus culture was suggestive of brain abscess caused by C. bantiana. We report a culture proven case of C. bantiana emphasizing on specific MRI features which are critical in differentiating fungal from nonfungal brain abscesses

An Experimental Investigation of the Scaling of Columnar Joints

Columnar jointing is a fracture pattern common in igneous rocks in which cracks self-organize into a roughly hexagonal arrangement, leaving behind an ordered colonnade. We report observations of columnar jointing in a laboratory analog system, desiccated corn starch slurries. Using measurements of moisture density, evaporation rates, and fracture advance rates as evidence, we suggest an advective-diffusive system is responsible for the rough scaling behavior of columnar joints. This theory explains the order of magnitude difference in scales between jointing in lavas and in starches. We investigated the scaling of average columnar cross-sectional areas due to the evaporation rate, the analog of the cooling rate of igneous columnar joints. We measured column areas in experiments where the evaporation rate depended on lamp height and time, in experiments where the evaporation rate was fixed using feedback methods, and in experiments where gelatin was added to vary the rheology of the starch. Our results suggest that the column area at a particular depth is related to both the current conditions, and hysteretically to the geometry of the pattern at previous depths. We argue that there exists a range of stable column scales allowed for any particular evaporation rate.Comment: 12 pages, 11 figures, for supporting online movies, go to http://www.physics.utoronto.ca/nonlinear/movies/starch_movies.htm

The Cosmology of Massless String Modes

We consider the spacetime dynamics of a gas of closed strings in the context of General Relativity in a background of arbitrary spatial dimensions. Our motivation is primarily late time String Gas Cosmology, where such a spacetime picture has to emerge after the dilaton has stabilized. We find that after accounting for the thermodynamics of a gas of strings, only string modes which are massless at the self-dual radius are relevant, and that they lead to a dynamics which is qualitatively different from that induced by the modes usually considered in the literature. In the context of an ansatz with three large spatial dimensions and an arbitrary number of small extra dimensions, we obtain isotropic stabilization of these extra dimensions at the self-dual radius. This stabilization occurs for fixed dilaton, and is induced by the special string states we focus on. The three large dimensions undergo a regular Friedmann-Robertson-Walker expansion. We also show that this framework for late-time cosmology is consistent with observational bounds.Comment: 15 pages, no figures, references added (again