228 research outputs found
Sheared bioconvection in a horizontal tube
The recent interest in using microorganisms for biofuels is motivation enough
to study bioconvection and cell dispersion in tubes subject to imposed flow. To
optimize light and nutrient uptake, many microorganisms swim in directions
biased by environmental cues (e.g. phototaxis in algae and chemotaxis in
bacteria). Such taxes inevitably lead to accumulations of cells, which, as many
microorganisms have a density different to the fluid, can induce hydrodynamic
instabilites. The large-scale fluid flow and spectacular patterns that arise
are termed bioconvection. However, the extent to which bioconvection is
affected or suppressed by an imposed fluid flow, and how bioconvection
influences the mean flow profile and cell transport are open questions. This
experimental study is the first to address these issues by quantifying the
patterns due to suspensions of the gravitactic and gyrotactic green
biflagellate alga Chlamydomonas in horizontal tubes subject to an imposed flow.
With no flow, the dependence of the dominant pattern wavelength at pattern
onset on cell concentration is established for three different tube diameters.
For small imposed flows, the vertical plumes of cells are observed merely to
bow in the direction of flow. For sufficiently high flow rates, the plumes
progressively fragment into piecewise linear diagonal plumes, unexpectedly
inclined at constant angles and translating at fixed speeds. The pattern
wavelength generally grows with flow rate, with transitions at critical rates
that depend on concentration. Even at high imposed flow rates, bioconvection is
not wholly suppressed and perturbs the flow field.Comment: 19 pages, 9 figures, published version available at
http://iopscience.iop.org/1478-3975/7/4/04600
Dispersion of swimming algae in laminar and turbulent channel flows: consequences for photobioreactors
Shear flow significantly affects the transport of swimming algae in
suspension. For example, viscous and gravitational torques bias bottom-heavy
cells to swim towards regions of downwelling fluid (gyrotaxis). It is necessary
to understand how such biases affect algal dispersion in natural and industrial
flows, especially in view of growing interest in algal photobioreactors.
Motivated by this, we here study the dispersion of gyrotactic algae in laminar
and turbulent channel flows using direct numerical simulation (DNS) and the
analytical swimming dispersion theory of Bees and Croze (2010). Time-resolved
dispersion measures are evaluated as functions of the Peclet and Reynolds
numbers in upwelling and downwelling flows. For laminar flows, DNS results are
compared with theory using competing descriptions of biased swimming cells in
shear flow. Excellent agreement is found for predictions that employ
generalized-Taylor-dispersion. The results highlight peculiarities of
gyrotactic swimmer dispersion relative to passive tracers. In laminar
downwelling flow the cell distribution drifts in excess of the mean flow,
increasing in magnitude with Peclet number. The cell effective axial
diffusivity increases and decreases with Peclet number (for tracers it merely
increases). In turbulent flows, gyrotactic effects are weaker, but discernable
and manifested as non-zero drift. These results should significantly impact
photobioreactor design.Comment: 31 pages, 15 figures (includes supplementary materials
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Aspirin-Trigge red-Resolvin D1 reduces mucosal inflammation and promotes resolution in a murine model of acute lung injury
Acute Lung Injury (ALI) is a severe illness with excess mortality and no specific therapy. Protective actions were recently uncovered for docosahexaenoic acid -derived mediators, including D-series resolvins. Here, we used a murine self-limited model of hydrochloric acid-induced ALI to determine the effects of aspirin-triggered resolvin D1 (AT-RvD1) on mucosal injury. RvD1 and its receptor ALX/FPR2 were identified in murine lung after ALI. AT-RvD1 (~0.5 – 5 μg/kg) decreased peak inflammation, including bronchoalveolar lavage fluid (BALF) neutrophils by ~75%. Animals treated with AT-RvD1 had improved epithelial and endothelial barrier integrity and decreased airway resistance concomitant with increased BALF epinephrine levels. AT-RvD1 inhibited neutrophil-platelet heterotypic interactions by down-regulating both P-selectin and its ligand CD24. AT-RvD1 also significantly decreased levels of BALF pro-inflammatory cytokines, including IL-1β, IL-6, KC and TNF-α, and decreased NF-κB phosphorylated p65 nuclear translocation. Together, these findings indicate that AT-RvD1 displays potent mucosal protection and promotes catabasis after ALI
Elephants classify human ethnic groups by odor and garment color
Animals can benefit from classifying predators or other dangers into categories, tailoring their escape strategies to the type and nature of the risk. Studies of alarm vocalizations have revealed various levels of sophistication in classification [1-5]. In many taxa, reactions to danger are inflexible, but some species can learn the level of threat presented by the local population of a predator [6-8] or by specific, recognizable individuals [9-10]. Some species distinguish several species of predator, giving differentiated warning calls and escape reactions; here we explore an animal’s classification of sub-groups within a species. We show that elephants distinguish at least two Kenyan ethnic groups, and can identify them by olfactory and color cues independently. In the Amboseli ecosystem, Kenya, Maasai warriors demonstrate virility by spearing elephants (Loxodonta africana), but Kamba agriculturalists pose little threat. Elephants showed greater fear when they detected the scent of garments previously worn by Maasai than by Kamba men, and reacted aggressively to the color associated with Maasai warriors. Elephants are therefore able to classify members of a single species into sub-groups that pose different degrees of danger
Induced Pluripotent Stem Cell-Derived Retinal Pigmented Epithelium: A Comparative Study Between Cell Lines and Differentiation Methods
PurposeThe application of induced pluripotent stem cell-derived retinal pigmented epithelium (iPSC-RPE) in patients with retinal degenerative disease is making headway toward the clinic, with clinical trials already underway. Multiple groups have developed methods for RPE differentiation from pluripotent cells, but previous studies have shown variability in iPSC propensity to differentiate into RPE.MethodsThis study provides a comparison between 2 different methods for RPE differentiation: (1) a commonly used spontaneous continuously adherent culture (SCAC) protocol and (2) a more rapid, directed differentiation using growth factors. Integration-free iPSC lines were differentiated to RPE, which were characterized with respect to global gene expression, expression of RPE markers, and cellular function.ResultsWe found that all 5 iPSC lines (iPSC-1, iPSC-2, iPSC-3, iPSC-4, and iPSC-12) generated RPE using the directed differentiation protocol; however, 2 of the 5 iPSC lines (iPSC-4 and iPSC-12) did not yield RPE using the SCAC method. Both methods can yield bona fide RPE that expresses signature RPE genes and carry out RPE functions, and are similar, but not identical to fetal RPE. No differences between methods were detected in transcript levels, protein localization, or functional analyses between iPSC-1-RPE, iPSC-2-RPE, and iPSC-3-RPE. Directed iPSC-3-RPE showed enhanced transcript levels of RPE65 compared to directed iPSC-2-RPE and increased BEST1 expression and pigment epithelium-derived factor (PEDF) secretion compared to directed iPSC-1-RPE. In addition, SCAC iPSC-3-RPE secreted more PEDF than SCAC iPSC-1-RPE.ConclusionsThe directed protocol is a more reliable method for differentiating RPE from various pluripotent sources and some iPSC lines are more amenable to RPE differentiation
The Influence of Life History Milestones and Association Networks on Crop-Raiding Behavior in Male African Elephants
Factors that influence learning and the spread of behavior in wild animal populations are important for understanding species responses to changing environments and for species conservation. In populations of wildlife species that come into conflict with humans by raiding cultivated crops, simple models of exposure of individual animals to crops do not entirely explain the prevalence of crop raiding behavior. We investigated the influence of life history milestones using age and association patterns on the probability of being a crop raider among wild free ranging male African elephants; we focused on males because female elephants are not known to raid crops in our study population. We examined several features of an elephant association network; network density, community structure and association based on age similarity since they are known to influence the spread of behaviors in a population. We found that older males were more likely to be raiders than younger males, that males were more likely to be raiders when their closest associates were also raiders, and that males were more likely to be raiders when their second closest associates were raiders older than them. The male association network had sparse associations, a tendency for individuals similar in age and raiding status to associate, and a strong community structure. However, raiders were randomly distributed between communities. These features of the elephant association network may limit the spread of raiding behavior and likely determine the prevalence of raiding behavior in elephant populations. Our results suggest that social learning has a major influence on the acquisition of raiding behavior in younger males whereas life history factors are important drivers of raiding behavior in older males. Further, both life-history and network patterns may influence the acquisition and spread of complex behaviors in animal populations and provide insight on managing human-wildlife conflict
Transcriptional response to interferon beta-1a treatment in patients with secondary progressive multiple sclerosis
Natural Single-Nucleosome Epi-Polymorphisms in Yeast
Epigenomes commonly refer to the sequence of presence/absence of specific epigenetic marks along eukaryotic chromatin. Complete histone-borne epigenomes have now been described at single-nucleosome resolution from various organisms, tissues, developmental stages, or diseases, yet their intra-species natural variation has never been investigated. We describe here that the epigenomic sequence of histone H3 acetylation at Lysine 14 (H3K14ac) differs greatly between two unrelated strains of the yeast Saccharomyces cerevisiae. Using single-nucleosome chromatin immunoprecipitation and mapping, we interrogated 58,694 nucleosomes and found that 5,442 of them differed in their level of H3K14 acetylation, at a false discovery rate (FDR) of 0.0001. These Single Nucleosome Epi-Polymorphisms (SNEPs) were enriched at regulatory sites and conserved non-coding DNA sequences. Surprisingly, higher acetylation in one strain did not imply higher expression of the relevant gene. However, SNEPs were enriched in genes of high transcriptional variability and one SNEP was associated with the strength of gene activation upon stimulation. Our observations suggest a high level of inter-individual epigenomic variation in natural populations, with essential questions on the origin of this diversity and its relevance to gene x environment interactions
A Disease-Mediated Trophic Cascade in the Serengeti and its Implications for Ecosystem C
The removal of rinderpest had cascading effects on herbivore populations, fire, tree density, and even ecosystem carbon in the Serengeti ecosystem of East Africa
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