Vgl1, a multi-KH domain protein, is a novel component of the fission yeast stress granules required for cell survival under thermal stress

Multiple KH-domain proteins, collectively known as vigilins, are evolutionarily highly conserved proteins that are present in eukaryotic organisms from yeast to metazoa. Proposed roles for vigilins include chromosome segregation, messenger RNA (mRNA) metabolism, translation and tRNA transport. As a step toward understanding its biological function, we have identified the fission yeast vigilin, designated Vgl1, and have investigated its role in cellular response to environmental stress. Unlike its counterpart in Saccharomyces cerevisiae, we found no indication that Vgl1 is required for the maintenance of cell ploidy in Schizosaccharomyces pombe. Instead, Vgl1 is required for cell survival under thermal stress, and vgl1Δ mutants lose their viability more rapidly than wild-type cells when incubated at high temperature. As for Scp160 in S. cerevisiae, Vgl1 bound polysomes accumulated at endoplasmic reticulum (ER) but in a microtubule-independent manner. Under thermal stress, Vgl1 is rapidly relocalized from the ER to cytoplasmic foci that are distinct from P-bodies but contain stress granule markers such as poly(A)-binding protein and components of the translation initiation factor eIF3. Together, these observations demonstrated in S. pombe the presence of RNA granules with similar composition as mammalian stress granules and identified Vgl1 as a novel component that required for cell survival under thermal stress

Functional overlap between conserved and diverged KH domains in Saccharomyces cerevisiae SCP160

The K homology (KH) domain is a remarkably versatile and highly conserved RNA-binding motif. Classical KH domains include a characteristic pattern of hydrophobic residues, a Gly-X-X-Gly (GXXG) segment, and a variable loop. KH domains typically occur in clusters, with some retaining their GXXG sequence (conserved), while others do not (diverged). As a first step towards addressing whether GXXG is essential for KH-domain function, we explored the roles of conserved and diverged KH domains in Scp160p, a multiple-KH-domain-containing protein in Saccharomyces cerevisiae. We specifically wanted to know (1) whether diverged KH domains were essential for Scp160p function, and (2) whether diverged KH domains could functionally replace conserved KH domains. To address these questions, we deleted and/or interchanged conserved and diverged KH domains of Scp160p and expressed the mutated alleles in yeast. Our results demonstrated that the answer to each question was yes. Both conserved and diverged KH domains are essential for Scp160p function, and diverged KH domains can function in place of conserved KH domains. These findings challenge the prevailing notions about the requisite features of a KH domain and raise the possibility that there may be more functional KH domains in the proteome than previously appreciated

Personality traits, risky riding behaviors and crash-related outcomes: findings from 5,778 cyclists in 17 countries

The last few years have brought about a series of substantial changes for mobility on two wheels, especially if the impact of the COVID-19 pandemic is considered as a relevant fact for transportation dynamics [1,2]. Social distancing recommendations have promoted the use of individual transportation systems instead of massive transportations means. Consequently, riding a bike for urban trips has become increasingly prevalent in many countries [3-5]. Besides an opportunity to make urban mobility more active and sustainable, this panorama poses the challenge to prevent that, along with its growing use, bicycle crashes ---and their consequences-might continue to increase. In this regard, recent studies have emphasized the role of individual differences and personality-related factors as potential issues influencing both cycling behaviors and traffic crashes suffered while riding [6,7]

Cross-culturally approaching the cycling behaviour questionnaire (CBQ) : Evidence from 19 countries

Peer reviewe

Speaking of Contradiction

Whereas McGovern calls for a moratorium on the ever increasing (ab)use of the word ‘contradiction’, principally because scholars of work and employment fail to connect different levels of analysis and/or demonstrate how and why contradiction(s) lead to widespread instability and upheaval, it can be demonstrated how both can be achieved through the ‘system, society, dominance’ framework. In what follows, the empirical focus is on the safety-critical work of airport ground service providers (GSPs), where key elements of the employment relationship embody contradictions that can be traced to the (sub-)system (mode of production) of a Single European Aviation Market (SEAM) that is now dominated by low fares airlines (LFAs). Instead of a moratorium, scholars of work and employment need to reconnect with society and theoretically ground their analysis in a (capitalist) system beset with contradictions between the forces and relations of production

Short RNA Guides Cleavage by Eukaryotic RNase III

In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been demonstrated. Here we show that Rnt1p, the yeast orthologue of the bacterial dsRNA-specific RNase III, can bind short RNA transcripts and use them as guides for sequence-specific cleavage. Target cleavage occurred at a constant distance from the Rnt1p binding site, leaving the guide RNA intact for subsequent cleavage. Our results indicate that RNase III may trigger sequence-specific RNA degradation independent of the RNAi machinery, and they open the road for a new generation of precise RNA silencing tools that do not trigger a dsRNA-mediated immune response