Dark energy records in lensed cosmic microwave background

We consider the weak lensing effect induced by linear cosmological perturbations on the cosmic microwave background (CMB) polarization anisotropies. We find that the amplitude of the lensing peak in the BB mode power spectrum is a faithful tracer of the dark energy dynamics at the onset of cosmic acceleration. This is due to two reasons. First, the lensing power is non-zero only at intermediate redshifts between the observer and the source, keeping record of the linear perturbation growth rate at the corresponding epoch. Second, the BB lensing signal is expected to dominate over the other sources. The lensing distortion on the TT and EE spectra do exhibit a similar dependence on the dark energy dynamics, although those are dominated by primary anisotropies. We investigate and quantify the effect by means of exact tracking quintessence models, as well as parameterizing the dark energy equation of state in terms of the present value ($w_{0}$) and its asymptotic value in the past ($w_{\infty}$); in the interval allowed by the present constraints on dark energy, the variation of $w_{\infty}$ induces a significant change in the BB mode lensing amplitude. A Fisher matrix analysis, under conservative assumptions concerning the increase of the sample variance due to the lensing non-Gaussian statistics, shows that a precision of order 10% on both $w_{0}$ and $w_{\infty}$ is achievable by the future experiments probing a large sky area with angular resolution and sensitivity appropriate to detect the lensing effect on the CMB angular power spectrum. These results show that the CMB can probe the differential redshift behavior of the dark energy equation of state, beyond its average.Comment: New version including substantial text change, three more figures and two more table

Compact strain-sensitive flexible photonic crystals for sensors

A promising fabrication route to produce absorbing flexible photonic crystals is presented, which exploits self-assembly during the shear processing of multi-shelled polymer spheres. When absorbing material is incorporated in the interstitial space surrounding high-refractive-index spheres, a dramatic enhancement in the transmission edge on the short-wavelength side of the band gap is observed. This effect originates from the shifting optical field spatial distribution as the incident wavelength is tuned around the band gap, and results in a contrast up to 100 times better than similar but nonabsorbing photonic crystals. An order-of-magnitude improvement in strain sensitivity is shown, suggesting the use of these thin films in photonic sensors

Modular Use of the Uniquely Small Ring A of Mersacidin Generates the Smallest Ribosomally Produced Lanthipeptide

Mersacidin is an antimicrobial class II lanthipeptide. Lanthipeptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs), characterized by intramolecular lanthionine rings. These rings give lanthipeptides their bioactive structure and stability. RiPPs are produced from a gene cluster that encodes a precursor peptide and its dedicated unique modification enzymes. The field of RiPP engineering aims to recombine modification enzymes from different RiPPs to modify new substrates, resulting in new-to-nature molecules with novel or improved functionality. The enzyme MrsM from the mersacidin gene cluster installs the four lanthionine rings of mersacidin, including the uniquely small ring A. By applying MrsM in RiPP engineering, this ring could be installed in linear peptides to achieve stabilization by a very small lanthionine or to create small lanthionine-stabilized modules for chemical modification. However, the formation of unique intramolecular structures like that of mersacidin's ring A can be very stringent. Here, the formation of ring A of mersacidin is characterized by mutagenesis. A range of truncated mersacidin variants was made to identify the smallest possible construct in which this ring could still be formed. Additionally, mutants were created to study the flexibility of ring A formation. It was found that although the formation of ring A is stringent, it can be formed in a core peptide as small as five amino acids. The truncated mersacidin core peptide CTFAL is the smallest ribosomally produced lanthipeptide reported to date, and it has exciting prospects as a new module for application in RiPP engineering

Braneworld inflation from an effective field theory after WMAP three-year data

In light of the results from the WMAP three-year sky survey, we study an inflationary model based on a single-field polynomial potential, with up to quartic terms in the inflaton field. Our analysis is performed in the context of the Randall-Sundrum II braneworld theory, and we consider both the high-energy and low-energy (i.e. the standard cosmology case) limits of the theory. We examine the parameter space of the model, which leads to both large-field and small-field inflationary type solutions. We conclude that small field inflation, for a potential with a negative mass square term, is in general favored by current bounds on the tensor-to-scalar perturbation ratio rs.Comment: 11 pages, 5 figures; references updated and a few comments added; final version to appear in Phys. Rev.

Pressure Support vs. Thermal Broadening in the Lyman-alpha Forest I: Effects of the Equation of State on Longitudinal Structure

In the low density intergalactic medium (IGM) that gives rise to the Lyman-alpha forest, gas temperature and density are tightly correlated. The velocity scale of thermal broadening and the Hubble flow across the gas Jeans scale are of similar magnitude (Hlambda_J ~ sigma_th). To separate the effects of gas pressure support and thermal broadening on the Lya forest, we compare spectra extracted from two smoothed particle hydrodynamics (SPH) simulations evolved with different photoionization heating rates (and thus different Jeans scales), imposing different temperature-density relations on the evolved particle distributions. The turnover scales in the flux power spectrum and flux autocorrelation function are determined mainly by thermal broadening rather than pressure. However, the insensitivity to pressure arises partly from a cancellation effect with a sloped temperature-density relation (T ~ rho^{0.6} in our simulations): the high density peaks in the colder, lower pressure simulation are less smoothed by pressure support than in the hotter simulation, and it is this higher density gas that experiences the strongest thermal broadening. Changes in thermal broadening and pressure support have comparably important effects on the flux probability distribution (PDF), which responds directly to the gas overdensity distribution rather than the scale on which it is smooth. Tests on a lower resolution simulation show that our statistical results are converged even at this lower resolution. While thermal broadening generally dominates the longitudinal structure in the Lya forest, we show in Paper II that pressure support determines the transverse coherence of the forest observed towards close quasar pairs. [ABRIDGED]Comment: 12 figures, MNRAS in pres

Characterization of Leader Processing Shows That Partially Processed Mersacidin Is Activated by AprE After Export

The ribosomally synthesized and post-translationally modified peptide mersacidin is a class II lanthipeptide with good activity against Gram-positive bacteria. The intramolecular lanthionine rings, that give mersacidin its stability and antimicrobial activity, are specific structures with potential applications in synthetic biology. To add the mersacidin modification enzymes to the synthetic biology toolbox, a heterologous expression system for mersacidin in Escherichia coli has recently been developed. While this system was able to produce fully modified mersacidin precursor peptide that could be activated by Bacillus amyloliquefaciens supernatant and showed that mersacidin was activated in an additional proteolytic step after transportation out of the cell, it lacked a mechanism for clean and straightforward leader processing. Here, the protease responsible for activating mersacidin was identified and heterologously produced in E. coli, improving the previously reported heterologous expression system. By screening multiple proteases, the stringency of proteolytic activity directly next to a very small lanthionine ring is demonstrated, and the full two-step proteolytic activation of mersacidin was elucidated. Additionally, the effect of partial leader processing on diffusion and antimicrobial activity is assessed, shedding light on the function of two-step leader processing

Semisynthetic Macrocyclic Lipo-lanthipeptides Display Antimicrobial Activity Against Bacterial Pathogens

[Image: see text] A large number of antimicrobial peptides depend on intramolecular disulfide bonds for their biological activity. However, the relative instability of disulfide bonds has limited the potential of some of these peptides to be developed into therapeutics. Conversely, peptides containing intramolecular (methyl)lanthionine-based bonds, lanthipeptides, are highly stable under a broader range of biological and physical conditions. Here, the class-II lanthipeptide synthetase CinM, from the cinnamycin gene cluster, was employed to create methyllanthionine stabilized analogues of disulfide-bond-containing antimicrobial peptides. The resulting analogues were subsequently modified in vitro by adding lipid tails of variable lengths through chemical addition. Finally, the created compounds were characterized by MIC tests against several relevant pathogens, killing assays, membrane permeability assays, and hemolysis assays. It was found that CinM could successfully install methyllanthionine bonds at the intended positions of the analogues and that the lipidated macrocyclic core peptides have bactericidal activity against tested Gram-positive and Gram-negative pathogenic bacteria. Additionally, fluorescence microscopy assays revealed that the lipidated compounds disrupt the bacterial membrane and lyse bacterial cells, hinting toward a potential mode of action. Notably, the semisynthesized macrocyclic lipo-lanthipeptides show low hemolytic activity. These results show that the methods developed here extend the toolbox for novel antimicrobial development and might enable the further development of novel compounds with killing activity against relevant pathogenic bacteria

Synthesis and Characterization of Heterodimers and Fluorescent Nisin Species by Incorporation of Methionine Analogues and Subsequent Click Chemistry

Noncanonical amino acids form a highly diverse pool of building blocks that can render unique physicochemical properties to peptides and proteins. Here, four methionine analogues with unsaturated and varying side chain lengths were successfully incorporated at four different positions in nisin in Lactococcus lactis through force feeding. This approach allows for residue-specific incorporation of methionine analogues into nisin to expand their structural diversity and alter their activity profiles. Moreover, the insertion of methionine analogues with biorthogonal chemical reactivity, e.g., azidohomoalanine and homopropargylglycine, provides the opportunity for chemical coupling to functional moieties and fluorescent probes as well as for intermolecular coupling of nisin variants. All resulting nisin conjugates retained antimicrobial activity, which substantiates the potential of this method as a tool to further study its localization and mode of action

Decaying Sterile Neutrinos as a Heating Source in the Milky Way Center

Recent Chandra and Newton observations indicate that there are two-temperature components ($T \sim$ 8 keV, 0.8 keV) of the diffuse x-rays emitted from deep inside the center of Milky Way. We show that this can be explained by the existence of sterile neutrinos, which decay to emit photons that can be bound-free absorbed by the isothermal hot gas particles in the center of Milky Way. This model can account for the two-temperature components naturally as well as the energy needed to maintain the $\sim$ 8 keV temperature in the hot gas. The predicted sterile neutrino mass is between 16-18 keV.Comment: Accepted by MNRAS with minor correction

High-Redshift Cosmography

We constrain the parameters describing the kinematical state of the universe using a cosmographic approach, which is fundamental in that it requires a very minimal set of assumptions (namely to specify a metric) and does not rely on the dynamical equations for gravity. On the data side, we consider the most recent compilations of Supernovae and Gamma Ray Bursts catalogues. This allows to further extend the cosmographic fit up to $z = 6.6$, i.e. up to redshift for which one could start to resolve the low z degeneracy among competing cosmological models. In order to reliably control the cosmographic approach at high redshifts, we adopt the expansion in the improved parameter $y = z/(1+z)$. This series has the great advantage to hold also for $z > 1$ and hence it is the appropriate tool for handling data including non-nearby distance indicators. We find that Gamma Ray Bursts, probing higher redshifts than Supernovae, have constraining power and do require (and statistically allow) a cosmographic expansion at higher order than Supernovae alone. Exploiting the set of data from Union and GRBs catalogues, we show (for the first time in a purely cosmographic approach parametrized by deceleration $q_0$, jerk $j_0$, snap $s_0$) a definitively negative deceleration parameter $q_0$ up to the 3$\sigma$ confidence level. We present also forecasts for realistic data sets that are likely to be obtained in the next few years.Comment: 16 pages, 6 figures, 3 tables. Improved version matching the published one, additional comments and reference