Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit

Due to an increasing risk of chemical contamination upon the application of synthetic fungicides to preserve fresh fruits and vegetables, essential oils are gaining increasing attentions. In this research, besides chemical analysis of the essential oils of three Satureja species (S. hortensis, S. spicigera, and S. khuzistanica) by GC-MS, their fungicidal and/or fungistatic effects on postharvest pathogens of strawberry were investigated. Essential oils were extracted by means of hydro-distillation and afterwards GC/MS analysis was performed to identify their components. Carvacrol, γ-terpinene and p-cymene were detected as the repeating main constituents of the spices, while thymol and carvacrol methyl ether were found as major components only in S. spicigera oil. In vitro results showed that at the maximum concentration, the essential oils did not possess fungicidal effects on Aspergillus niger but they exhibited fungicidal activities against Penicillium digitatum, Botrytis cinerea and Rhizopus stolonifer. However, S. khuzistanica was the strongest oil in fungicidal activity. S. hortensis oil was more effective than S. spicigera against B. cinerea whereas S. spicigera oil showed stronger fungicidal activity against R. stolonifer. In conclusion, essential oils isolated from three savory species could be suitable for applications in the food industry to control molds and improve the safety of fruits and vegetables. © 2015 Elsevier B.V

Interaction of IBA and NAA with enzymes in root induction of Crocus sativus (L)

Effect of auxin type on some antioxidative enzymes during in vitro root induction of saffron (Crocus sativus L.) was investigated to reveal the variation of activities and isoenzyme banding patterns during root induction of saffron (C. sativus L.). Indole-3- butyric acid (IBA) and naphtalene acetic acid (NAA) at different concentrations were added to the solidified MS or B5 medium. Corm explants of saffron were placed on root inducing media and enzyme levels in explants prior or after adventitious root formation were measured and compared. Percentage of root formation on B5 medium containing 2.46 ìM IBA was highest; however the maximum number of root per explant and per cm2 observed on MS medium with 19.6 ìM IBA. Significant changes occurred in the activities of these enzymes. Some polymorphisms occurred in patterns of lactate dehydrogenase and esterase during root induction in different treatments

Effect of interleukin-22 on immunogenicity of DNA vaccine encoding TSA gene of leishmania major in BALB/c mice

Background and purpose: Previous Research shows the use of plasmids containing genes TSA to be useful as vaccines for Leishmania major. Recently, the role of interleukin-22 (IL-22) in tissue repair has been demonstrated. In this research, the effect of IL-22 on encoding TSA gene of Leishmania major in BALB/c mice was assessed

Three-dimensional cohesive fracture modeling of non-planar crack growth using adaptive FE technique

AbstractIn this paper, the three-dimensional adaptive finite element modeling is presented for cohesive fracture analysis of non-planer crack growth. The technique is performed based on the Zienkiewicz–Zhu error estimator by employing the modified superconvergent patch recovery procedure for the stress recovery. The Espinosa–Zavattieri bilinear constitutive equation is used to describe the cohesive tractions and displacement jumps. The 3D cohesive fracture element is employed to simulate the crack growth in a non-planar curved pattern. The crack growth criterion is proposed in terms of the principal stress and its direction. Finally, several numerical examples are analyzed to demonstrate the validity and capability of proposed computational algorithm. The predicted crack growth simulation and corresponding load-displacement curves are compared with the experimental and other numerical results reported in literature

Physarum Inspired Dynamics to Solve Semi-Definite Programs

Physarum Polycephalum is a Slime mold that can solve the shortest path problem. A mathematical model based on the Physarum's behavior, known as the Physarum Directed Dynamics, can solve positive linear programs. In this paper, we will propose a Physarum based dynamic based on the previous work and introduce a new way to solve positive Semi-Definite Programming (SDP) problems, which are more general than positive linear programs. Empirical results suggest that this extension of the dynamic can solve the positive SDP showing that the nature-inspired algorithm can solve one of the hardest problems in the polynomial domain. In this work, we will formulate an accurate algorithm to solve positive and some non-negative SDPs and formally prove some key characteristics of this solver thus inspiring future work to try and refine this method

Reduced Phagocytic Capacity of Blood Monocyte/Macrophages in Tuberculosis Patients Is Further Reduced by Smoking.

Tuberculosis (TB) and tobacco use are two major alarming global health issues posing immense threats to human populations. Mycobacterium tuberculosis (MTB) by activation of macrophages could induce the sequences of cells activation and releases of inflammatory cytokines such as CXCL-8, Il-12 and TNF-α which in turn induces the immune system network. However no information is available on other activity of cells by MTB and smoking. In the current study we aimed to investigate the serum levels TNF-a, CXCL-8 and phagocytosis capacity in tuberculosis patients with and without smoking. 103 subjects entered the study including 61 new diagnosed pulmonary TB patients (23 smokers and 38 nonsmokers) and 42 control healthy subjects. The phagocytosis of fluorescein isothiocyanate dextran (FITC-dextran) in blood monocytes/macrophages through flowcytometry was assessed. Serum levels of TNF-a and CXCL-8 were analyzed by ELISA methods. A lower percentage of cells from TB patients who smoked [50.29% (43.4-57.2), p<0.01] took up FITC-dextran after 2h compared to non-smoking TB subjects [71.62% (69.2-74.1)] and healthy cases [97.45% (95.9-99.1). Phagocytic capacity was inversely correlated with cigarette smoking as measured by pack years (r=-0.73, p<0.001). The serum levels of TNF-a and CXCL-8 were significantly higher in the TB patients who smoked compared to the TB non-smoker group (p<0.001, p<0.01 respectively). Blood monocytes/macrophages from TB patients have reduced phagocytic capacity which is further reduced in TB patients who smoke. Smoking enhanced serum levels of TNF-a and CXCL-8 suggesting a greater imbalance between the proinflammatory and anti-inflammatory factors in these patients