Comparison of photocatalytic systems including silver and titanium dioxide nanoparticles efficiencies for the E. coli removal from drinking water

The removal and destruction of organic contaminants in groundwater can be addressed through the impregnation of adsorbents with photoactive catalysts. In this paper removal trend of E. coli from drinking water examined by nano silver and nano titanium dioxide. To perform this, four different concentration of silver nano particles and titanium dioxide under UV radiation (with 247 nm- wavelength) used. The results showed the nano particles of silver and titanium reach to 100% disinfection efficiency at the concentrations of 0.4 mg/l (with 20 minutes contact time) and 0.8 mg/l (with 40 minutes contact time), respectively. For equal amounts of disinfectant and equal number of E. coli colonies in drinking water, disinfection potential for (nAg + V) is significantly higher than (nTiO2 + UV). When the nano particles concentration increases, the disinfection rate rises, and it was higher and faster done by the nano silver particles comparing to nano-titanium particles (Pvalue < 0.05, R²= 0.705)

Implementasi Kebijakan Pengelolaan Wilayah Pesisir Pada Kawasan Pemanfaatan Umum Dan Konservasi Kota Semarang

The implementation of policies on the management of coastal in the area of general used and conservation at semarang is planning coordination, utilization, monitoring, and control of coastal resources of the Government and Local Government, between sectors, between terrestrial and marine ecosystems, as well as between science and management to improve the welfare of the people. The purpose of this research is to analyze Policy For Coastal Management Policy Implementation in the region common Utilization and Conservation Semarang with 5 indicators that can be used to assess the quality of policy output, namely Access, Coverage, Frequency, Service Delivery and Accountability. Researchers analyzed factors that support and hinder the implementation of coastal zone management policy with 4 factors was found in the field. 4 factors are environmental, Technology, program plan and Socio economic Conditions. The results of this research is that the implementation of policies on the management of coastal in the area of general used and conservation at semarang is good enough but still not optimal, because there are 3 indicators are encountering many obstacles for the Implementation. Still need for improvement in indicators Access, Coverage and Frequency

Development of Indirect Immunofluorescence Technique for the Identification of MRC5 Working Seed Cell

Diploid and continuous cell lines are used to propagate viral vaccines. At Human Viral Vaccine Department of Razi Vaccine and Serum Research Institute, MRC5 diploid cell is used for the development of live attenuated measles, mumps, rubella, and three types of poliovirus vaccines.  Additionally, three continuous cell lines (i.e., RK13, HeLa, and Vero) are applied in quality control tests. Accordingly, cell cross-contamination can occur at cell culture labs, hence controlling the identity and specificity of cells is essential. Indirect immunofluorescence is a sensitive, specific, and simple test for cell identification. The present study was designed to develop the in-house indirect immunofluorescence test (IIF) as follows: homemade polyclonal anti-MRC5 serum was prepared in rabbits, and cross-reactive antibodies to RK13, HeLa, and Vero cells were eliminated. The diploid and continuous cell lines were fixed on Teflon slide using cold methanol and acetone. The reproducibility of the in-house IIF test was evaluated using the agreement Kappa test.  The purity of the three batches of MRC5 working seed cell at Human Viral Vaccine Department of Razi institute was verified using IIF and no contamination with continuous cell lines was detected

The EGS Grading Scale For Skin And Soft Tissue Infections Is Predictive Of Poor Outcomes : A Multicenter Validation Study

Introduction: Over the last five years, the American Association for the Surgery of Trauma (AAST) has developed grading scales for Emergency General Surgery (EGS) diseases. In a prior validation study using diverticulitis, the grading scales were predictive of complications and length of stay. As EGS encompasses diverse diseases, the purpose of this study was to validate the grading scale concept against a different disease process with a higher associated mortality. We hypothesized that the grading scale would be predictive of complications, length of stay and mortality in skin and soft tissue infections (STI). Methods: This multi-institutional trial encompassed 12 centers. Data collected included demographic variables, disease characteristics and outcomes such as mortality, overall complications, hospital and ICU length of stay. The EGS scale for STI was used to grade each infection and two surgeons graded each case to evaluate inter-rater reliability. Results: 1170 patients were included in this study. Inter-rater reliability was moderate (kappa coefficient 0.472-0.642, with 64-76% agreement). Higher grades (IV and V) corresponded to significantly higher LRINEC scores when compared with lower EGS grades. Patients with grade IV and V STI had significantly increased odds of all complications, as well as ICU and overall length of stay. These associations remained significant in logistic regression controlling for age, gender, comorbidities, mental status and hospital-level volume. Grade V disease was significantly associated with mortality as well. Conclusion: This validation effort demonstrates that Grade IV and V STI are significantly predictive of complications, hospital length of stay and mortality. Though predictive ability does not improve linearly with STI grade, this is consistent with the clinical disease process, in which lower grades represent cellulitis and abscess and higher grades are invasive infections. This second validation study confirms the EGS grading scale as predictive, and easily used, in disparate disease processes

Ventilator-associated pneumonia in children after cardiac surgery in The Netherlands

We conducted a retrospective cohort study in an academic tertiary care center to characterize ventilator-associated pneumonia (VAP) in pediatric patients after cardiac surgery in The Netherlands. All patients following cardiac surgery and mechanically ventilated for ≥24 h were included. The primary outcome was development of VAP. Secondary outcomes were duration of mechanical ventilation and length of ICU stay. A total of 125 patients were enrolled. Their mean age was 16.5 months. The rate of VAP was 17.1/1,000 mechanical ventilation days. Frequently found organisms were Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Pseudomonas aeruginosa. Patients with VAP had longer duration of ventilation and longer ICU stay. Risk factors associated with the development of VAP were a PRISM III score of ≥10 and transfusion of fresh frozen plasma. The mean VAP rate in this population is higher than that reported in general pediatric ICU populations. Children with VAP had a prolonged need for mechanical ventilation and a longer ICU sta