The removal and destruction of organic contaminants in groundwater can be addressed through the impregnation of adsorbents with photoactive catalysts. In this paper removal trend of E. coli from drinking water examined by nano silver and nano titanium dioxide. To perform this, four different concentration of silver nano particles and titanium dioxide under UV radiation (with 247 nm- wavelength) used. The results showed the nano particles of silver and titanium reach to 100% disinfection efficiency at the concentrations of 0.4 mg/l (with 20 minutes contact time) and 0.8 mg/l (with 40 minutes contact time), respectively. For equal amounts of disinfectant and equal number of E. coli colonies in drinking water, disinfection potential for (nAg + V) is significantly higher than (nTiO2 + UV). When the nano particles concentration increases, the disinfection rate rises, and it was higher and faster done by the nano silver particles comparing to nano-titanium particles (Pvalue < 0.05, R²= 0.705)