The two-sex life table and predation rate of Oenopia conglobata contaminata (Col.: Coccinellidae) feeding on pomegranate green aphid, Aphis punicae (Hem.: Aphididae), under laboratory conditions

The ladybird species Oenopia conglobata contaminata (Menetries) is one of the major predators of pests in the orchards across Iran. The life history and predation rate of this predator were studied under laboratory conditions with 27.5 ± 2oC, 55 ± 5 RH and 16: 8 h (L: D) photoperiod to 6-cm petri dishes. To study the demography, a cohort containing about 100 eggs with less than 24h of age was chosen. Ladybeetle larvae were fed daily on the immature stages of pomegranate green aphids, Aphis punicae Pass. The daily fertility, immature developmental time, mortality and predation rate data were analyzed using age-stage, two-sex life table analysis - MSChart software. Means and standard errors of population growth parameters were calculated by bootstrap method. To determine the predation rate, the first-instar beetle larvae were fed on the third and forth developmental stages of A. punicae. The adult ladybirds were grouped in pairs of opposite sexes to investigate their predation rates on daily basis. The intrinsic rate of increase (rm), finite rate of increase(λ) , mean generation time (T0) and net reproductive rate )R0( were 0.181 d-1, 1.198 d-1, 30.58 d and 251.65 offspring per individual respectively. Predation rate of different stages of forth-instar larvae and both adult sexes were 49.56, 95 and 125, aphids respectively, and the average predation rate (C0) was 118 aphids that shows a high rate of predation on the aphid population

Effect of temperature on life table parameters of Diaphania indica (Lep.: Pyralidae) under laboratory conditions

The cucumber moth, Diaphania indica (Saunders), is a tropical and sub-tropical pest on cucurbits and a key pest of greenhouse crops in Jiroft region of Iran. The effect of temperature on life table parameters of this pest was investigated in a growth chamber at four constant temperatures i.e. 20, 25, 30 and 35 ºC on Cucumis sativus L. The net reproductive rates (R0) were found to be 68.19, 120.977, 64.05 and 21.23, respectively. The intrinsic rates of increase (rm) were 0.0619, 0.1746, 0.1934 and 0.1491, and mean generation times (T) were 69.063, 27.45, 21.49 and 20.44, respectively. According to the results, for the intrinsic rate of increase (rm), finite rate of increase (λ) and intrinsic birth rate (b), the optimum temperature was 30 ºC and the least suitable temperature was 20 ºC

Math teachers' beliefs, practices, and belief change in implementing problem based learning in Qatari primary governmental school

This study explored math teachers' beliefs regarding their roles, practices and perceived change in implementing Problem-Based Learning in Qatar's primary government schools. Multiple sources of qualitative data were generated including metaphors, lesson plans and interviews with seventeen math teachers. Although teachers considered PBL as an effective method benefiting student learning and they demonstrated progress in changing their beliefs moving from subject to didactic dimension through PBL implementation, their practices remained partially aligned with their perceived belief changes. This discrepancy could be attributed to several encountered challenges, including teachers' insecurity and lack of confidence, difficulty in facilitating student collaboration, structural constraints, additional workload, and the lack of school and peer support. Results suggest the need for different types of "problems" and approaches such as more direct instruction, and higher feasibility in teachers' autonomy when implementing PBL in primary education.Scopu

Crystal-Induced Podocytopathy Producing Collapsing Focal Segmental Glomerulosclerosis in Monoclonal Gammopathy of Renal Significance: A Case Report

Glomeruloesclerosis focal y segmentaria; Queratopatía cristalina; PodocitopatíaGlomeruloesclerosi focal i segmentària; Queratopatia cristal·lina; PodocitopatiaFocal and segmental glomerulosclerosis; Crystalline keratopathy; PodocytopathyMonoclonal gammopathy–associated crystalline podocytopathy causing collapsing focal segmental glomerulosclerosis (FSGS) is very rare and has been associated with pamidronate therapy. We present the case of a 53-year-old man with vision loss secondary to corneal crystals deposition, nephrotic-range proteinuria, and reduced glomerular filtration rate without associated comorbid conditions. Two kidney biopsies were initially reported as primary FSGS but the patient did not respond to high-dose corticosteroid immunosuppression therapy. Re-review of biopsies with additional electron microscopy analysis revealed crystalline inclusions in podocytes leading to collapsing FSGS. Subsequent workup revealed an immunoglobulin G κ serum monoclonal protein. Bone marrow biopsy revealed 5% κ-restricted plasma cells with cytoplasmic crystalline inclusions. To our knowledge, this is the first case of monoclonal gammopathy of clinical significance manifesting as crystalline podocytopathy leading to collapsing FSGS and keratopathy leading to vision loss. Crystalline podocytopathy should be considered in the differential diagnosis of collapsing glomerulopathy, and careful ultrastructural examination of the kidney biopsy specimen is crucial to establish this diagnosis.The authors declare that they have no relevant financial interests

Predatory efficiency of Hippodamia variegata (Col.: Coccinellidae) on common pistachio psylla, Agonoscena pistaciae (Hem.: Aphalaridae), under laboratory conditions

Common pistachio psylla, Agonoscena pistaciae Burckhardt & Lauterer, is the most economically important species among the psyllid pests of pistachio in Iran. Hence, the possibility of using nonchemical control methods against A. pistaciae and the existence of its natural enemies need to be investigated. The spotted amber ladybird, Hippodamia variegata (Goeze), is one of the most effective predators in pistachio orchards. It is a highly polyphagous coccinellid that preys mainly on aphid and psyllid pests of pistachio trees. This study is intended to improve the knowledge about the predation capacity of A. pistaciae. The experiment was conducted under laboratory conditions of 25oC and 30oC, 55 ± 5 RH and 16: 8 L: D. Logistic regression was used to determine the type of functional response and nonlinear regression calculated the parameters of the rate of searching efficiency (a) and handling time (Th). The functional response on different density of prey at both temperatures was type II. The attack rate and handling time were 0.088 ± 0.012 h-1 and 0.0647 ± 0.011 h at 25ºC and 0.195 ± 0.031 h-1 and 0.0698 ± 0.056 h at 30ºC, respectively. The density and temperature affected the predation rate significantly. The data suggests that the H. variegata is a viable biological agent against A. pistaciae

Monitoring of Dead Sea water surface variation using multi-temporal satellite data and GIS.

Remote sensing (RS) and geographic information systems (GIS) are very useful for environmental-related studies, particularly in the field of surface water studies such as monitoring of lakes. The Dead Sea is exposed to very high evaporating process with considerable scarcity of water sources, thus leading to a remarkable shrinkage in its water surface area. The lake suffers from dry out due to the negative balance of water cycle during the previous four decades. This paper discusses the application of RS, GIS, and Global Positioning System to estimate the lowering and the shrinkage of Dead Sea water surface over the period 1810–2005. A set of multi-temporal remote sensing images were collected and processed to show the lakes aerial extend shrinkage from 1973 up to 2004. Remote sensing data were used to extract spatial information and to compute the surface areas for Dead Sea for various years. The current study aims at estimating the fluctuation of Dead Sea level over the study period with special emphasis on the environmental impact assessment that includes the degradation level of the Dead Sea. The results indicated that there is a decrease of 20 m in the level of the Dead Sea that has occurred during the study period. Further, the results showed that the water surface area of the Dead Sea has shrunk from 934.26 km2 in 1973 to 640.62 km2 in 2004

Laser microdissection and mass spectrometry–based proteomics aids the diagnosis and typing of renal amyloidosis

Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008–2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell–derived chemotaxin-2, fibrinogen-α chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and β-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure

Low- and high-molecular-weight urinary proteins as predictors of response to rituximab in patients with membranous nephropathy: a prospective study

Background. Selective urinary biomarkers have been considered superior to total proteinuria in predicting response to treatment and outcome in patients with membranous nephropathy (MN). Methods. We prospectively tested whether urinary (U) excretion of retinol-binding protein (RBP), α1-microglobulin (α1M), albumin, immunoglobulinIgG and IgM and/or anti-phospholipase 2 receptor (PLA2R) levels could predict response to rituximab (RTX) therapy better than standard measures in MN. We also correlated changes in antibodies to PLA2R with these urinary biomarkers. Results. Twenty patients with MN and proteinuria (P) >5 g/24 h received RTX (375 mg/m2 × 4) and at 12 months, 1 patient was in complete remission (CR), 9 were in partial remission (PR), 5 had a limited response (LR) and 4 were non-responders (NR). At 24 months, CR occurred in 4, PR in 12, LR in 1, NR in 2 and 1 patient relapsed. By simple linear regression analysis, UIgG at baseline (mg/24 h) was a significant predictor of change in proteinuria at 12 months (Δ urinary protein) (P = 0.04). In addition, fractional excretion (FE) of IgG, urinary alpha 1 microglobulin (Uα1M) (mg/24 h) and URBP (μg/24 h) were also predictors of response (P = 0.05, 0.04, and 0.03, respectively). On the other hand, UIgM, FEIgM, albumin and FE albumin did not predict response (P = 0.10, 0.27, 0.22 and 0.20, respectively). However, when results were analyzed in relation to proteinuria at 24 months, none of the U markers that predicted response at 12 m could predict response at 24 m (P = 0.55, 0.42, 0.29 and 0.20). Decline in anti-PLA2R levels was associated with and often preceded urinary biomarker response but positivity at baseline was not a predictor of proteinuria response. Conclusions. The results suggest that in patients with MN, quantification of low-, medium- and high-molecular-weight urinary proteins may be associated with rate of response to RTX, but do not correlate with longer term outcomes