49 research outputs found
Enhanced Support for High Intensity Users of the Criminal Justice System – an evaluation of mental health nurse input into Integrated Offender Management Services in the North East of England
The current UK Government’s focus on the development of services to manage and support offenders with mental health problems has resulted in a number of innovative project developments. This research examines a service development in the North East of England which co-located Mental Health nurses with two Integrated Offender Management teams. While not solving all problems, the benefits of co-location were clear – although such innovations are now at risk from government changes which will make Integrated Offender Management the responsibility of new providers without compelling them to co-operate with health services
Systems responses to progressive water stress in durum wheat
Durum wheat is susceptible to terminal drought which can greatly decrease grain yield. Breeding to improve crop yield is hampered by inadequate knowledge of how the physiological and metabolic changes caused by drought are related to gene expression. To gain better insight into mechanisms defining resistance to water stress we studied the physiological and transcriptome responses of three durum breeding lines varying for yield stability under drought. Parents of a mapping population (Lahn x Cham1) and a recombinant inbred line (RIL2219) showed lowered flag leaf relative water content, water potential and photosynthesis when subjected to controlled water stress time transient experiments over a six-day period. RIL2219 lost less water and showed constitutively higher stomatal conductance, photosynthesis, transpiration, abscisic acid content and enhanced osmotic adjustment at equivalent leaf water compared to parents, thus defining a physiological strategy for high yield stability under water stress. Parallel analysis of the flag leaf transcriptome under stress uncovered global trends of early changes in regulatory pathways, reconfiguration of primary and secondary metabolism and lowered expression of transcripts in photosynthesis in all three lines. Differences in the number of genes, magnitude and profile of their expression response were also established amongst the lines with a high number belonging to regulatory pathways. In addition, we documented a large number of genes showing constitutive differences in leaf transcript expression between the genotypes at control non-stress conditions. Principal Coordinates Analysis uncovered a high level of structure in the transcriptome response to water stress in each wheat line suggesting genome-wide co-ordination of transcription. Utilising a systems-based approach of analysing the integrated wheat's response to water stress, in terms of biological robustness theory, the findings suggest that each durum line transcriptome responded to water stress in a genome-specific manner which contributes to an overall different strategy of resistance to water stress
Dissection of early transcriptional responses to water stress in Arundo donax L. by unigene-based RNA-seq
Comparison of the Paddy Drying Process and Milling Quality between Re-circulating and Conventional Batch Type Dryers
Introduction High energy consumption and non-uniformity drying in conventional batch type dryer are the common problems in paddy dying industry. Non-uniformity drying causes to kernel breaking chance in the milling process. Using new dryers with better performance can solve the drying problem and energy saving. In this research, the operation of a re-circulating batch dryer was compared with a fixed bed batch dryer (conventional dryer) for paddy drying. Materials and Methods This research was done in a paddy milling factory in Ferydonkenar and deputy of Rice Research Institute of Iran, in Amol, Mazandaran province. Both re-circulating dryer and conventional batch type dryer were made by Khazar Electric Company in Amol- Iran and they had 5 tonnes capacity. In the re-circulating dryer, ambient air was warmed in the furnace and blown to drying zone inside of grain bin. Natural Gas (NG) was used for air warming in dryers. Warm air absorbed paddy moisture and pushed away from the dryer. Drying temperature ranges for re-circulating dryer and conventional dryer was 48-50 °C and 38-52°C, respectively. The paddy variety was one of the Iranian rice varieties as Tarom and initial moisture content of grains was 21% (w.b), it was decreased using drying to 8-9% (w.b) for milling process. Paddy moisture content was measured each 60-120 min by SUNCUE TD-6 portable moisture tester-Taiwan. Energy consumption calculated by fuel and electrical energy summation in each experiment. Natural Gas and electrical power consumption were measured by Gas and electric counters respectively. Drying time, paddy moisture change trend and energy consumption were investigated for paddy drying in each dryers. Also, milling ratio, breaking percent, whitening degree, and elongation rate after cooking were studied after the milling process for rice dried using national standard methods and deputy of Rice Research Institute facilities in Amol. Experimental samples were 150 g and husker (SATAKE THU35B), a whitener (SATAKE TMU05) and KETT C-100 were used for husking, whitening and whiteness degree, respectively. All Experiments were done with three replication and data analyzed using T- student method in 5% probability. Results and Discussion Results showed that re-circulating dryer caused to reduce 54.12 percent in drying time and energy saving in paddy drying in compare with conventional paddy dryers. The trend of moisture content changes was longer and over-drying occurred in lower layers in conventional batch type dryer compared to re-circulating dryer. Paddy drying was 20 hours more in batch type than the re-circulating dryer. It caused wasting time and energy consumption. Specific energy consumption for water evaporating in the re-circulating batch dryer was 3.9 MJ/kg water and it was 76.25 percent less than fixed bed batch dryer. After the drying process in both dryers, paddy moisture content was in range 8-9 percent (% w.b). Using re-circulating dryer did not have a significant effect on milling yield but it had a significant effect on broken rice. Broken rice decreased by 5 percent after the milling process when paddy dried by re-circulating. Uniformity of layers drying and normal heat stress in rice kernels in re-circulating dryer reduced broken rice in the milling process. Whiteness degree of rice dried using fixed bed dryer was 2.4 percent more than the re-circulating batch dryer. Also, rice dried had more elongation rate about 6.2 percent after cooking when paddy dried by conventional dryer. Conclusions Results of this paper showed that using of re-circulating dryer would decrease time and modify energy consumption in paddy drying. The costs of installation for the re-circulating batch dryer was about 5.3 times more than fixed bed batch dryer. It seems too expensive at first but considering energy and time-saving in the drying process and suitable effect on decreasing grain breakage in paddy milling, using of the re-circulating batch dryer is recommendable in rice milling factories
Elaboration and characterization of mullite-anorthite-albite porous ceramics prepared from Algerian kaolin
Abstract Mullite-anorthite-albite porous ceramic materials were successfully prepared by a solid-state reaction between kaolin clay and two different additives (CaCO3 and Na2CO3). The starting raw material was characterized by X-ray fluorescence, X-ray diffraction (XRD) and dynamic light scattering techniques. The effect of CaCO3 and Na2CO3 concentration (10 to 70 wt%) on structure, morphology and thermal properties of the obtained ceramics was investigated by XRD, scanning electron microscopy and differential scanning calorimetry (DSC) techniques. The XRD patterns showed that mullite (3Al2O3.2SiO2), anorthite (CaO.Al2O3.2SiO2) and albite (Na2O.Al2O3.6SiO2) were the main crystalline phases present in the materials. The morphology investigation revealed the porous texture of obtained ceramics characterized by the presence of sponge-like structure mainly due to the additive decomposition at high temperatures. The DSC results confirm the presence of four temperature regions related to the kaolin thermal transformations and the formation of minerals. The temperature and enthalpy of mineral formation are additive concentration dependent. As a result, the optimal content of additives which allowed the coexistence of the three phases, a sponge-like morphology, and high porosity without cracks corresponded to 15 wt% CaCO3, 15 wt% Na2CO3, and 70 wt% kaolin
Transcriptional profiling of the leaves of near-isogenic rice lines with contrasting drought tolerance at the reproductive stage in response to water deficit
Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress
Background: Plant roots are important organs to uptake soil water and nutrients, perceiving and transducing of soil water deficit signals to shoot. The current knowledge of drought stress transcriptomes in rice are mostly relying on comparative studies of diverse genetic background under drought. A more reliable approach is to use near-isogenic lines (NILs) with a common genetic background but contrasting levels of resistance to drought stress under initial exposure to water deficit. Here, we examined two pairs of NILs in IR64 background with contrasting drought tolerance. We obtained gene expression profile in roots of rice NILs under different levels of drought stress help to identify genes and mechanisms involved in drought stress. Results: Global gene expression analysis showed that about 55% of genes differentially expressed in roots of rice in response to drought stress treatments. The number of differentially expressed genes (DEGs) increased in NILs as the level of water deficits, increased from mild to severe condition, suggesting that more genes were affected by increasing drought stress. Gene onthology (GO) test and biological pathway analysis indicated that activated genes in the drought tolerant NILs IR77298-14-1-2-B-10 and IR77298-5-6-B-18 were mostly involved in secondary metabolism, amino acid metabolism, response to stimulus, defence response, transcription and signal transduction, and down-regulated genes were involved in photosynthesis and cell wall growth. We also observed gibberellic acid (GA) and auxin crosstalk modulating lateral root formation in the tolerant NILs. Conclusions: Transcriptome analysis on two pairs of NILs with a common genetic background (~97%) showed distinctive differences in gene expression profiles and could be effective to unravel genes involved in drought tolerance. In comparison with the moderately tolerant NIL IR77298-5-6-B-18 and other susceptible NILs, the tolerant NIL IR77298-14-1-2-B-10 showed a greater number of DEGs for cell growth, hormone biosynthesis, cellular transports, amino acid metabolism, signalling, transcription factors and carbohydrate metabolism in response to drought stress treatments. Thus, different mechanisms are achieving tolerance in the two tolerant lines
Comprehensive gene expression analysis of the NAC gene family under normal growth conditions, hormone treatment, and drought stress conditions in rice using near-isogenic lines (NILs) generated from crossing Aday Selection (drought tolerant) and IR64
The NAC (NAM, ATAF1/2 and CUC2) genes are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. We describe the rice (Oryza sativa) OsNAC genes expression profiles (GEPs) under normal and water-deficit treatments (WDTs). The GEPs of the OsNAC genes were analyzed in 25 tissues covering the entire life cycle of Minghui 63. High expression levels of 17 genes were demonstrated in certain tissues under normal conditions suggesting that these genes may play important roles in specific organs. We determined that 16 genes were differentially expressed under at least 1 phytohormone (NAA, GA3, KT, SA, ABA, and JA) treatment. To investigate the GEPs in the root, leaf, and panicle of three rice genotypes [e.g., 2 near-isogenic lines (NILs) and IR64], we used two NILs from a common genetic combination backcross developed by Aday Selection and IR64. WDTs were applied using the fraction of transpirable soil water at severe, mild, and control conditions. Transcriptomic analysis using a 44K oligoarray from Agilent was performed on all the tissue samples. We identified common and specific genes in all tissues from the two NILs under both WDTs, and the majority of the OsNAC genes that were activated were in the drought-tolerant IR77298-14-1-2-B-10 line compared with the drought-susceptible IR77298-14-1-2-B-13 or IR64. In IR77298-14-1-2-B-10, seventeen genes were very specific in their expression levels. Approximately 70 % of the genes from subgroups SNAC and NAM/CUC3 were activated in the leaf, but 37 % genes from subgroup SND were inactivated in the root compared with the control under severe stress conditions. These results provide a useful reference for the cloning of candidate genes from the specific subgroup for further functional analysi