Building up Steam as Consumers: Women, Rice Cookers and the Consumption of Everyday Household Goods in Japan

This chapter discusses the increasingly important role played by women as everyday consumers in post-war Japan, focusing on their consumption of household and kitchen appliances, specifically the electric rice cooker. Two key areas are explored. First, I investigate the development, production and consumption of this appliance. The electric rice cooker was developed by Japanese manufacturers from the mid-1950s, and was at the time unique to the Japanese manufacturing sector and the Japanese consumer market. It rapidly achieved significance in both domestic and export markets.1 The analysis will focus on the rice cooker’s development and impact in the Japanese market during the post-war years as a key example of the importance of everyday household appliances in the history of gender and consumption in Japan, impacting on women’s roles inside and outside the home. The chapter will show that although the rice cooker was in many ways a humble product, it had a revolutionary impact on Japanese women’s primary role as housewives. Second, the chapter places the case-study of the rice cooker within a broader context, discussing the role played by Japanese women as key consumers of appliances and as a gendered consumer group. It will explore the significance of housewives as a consumer group in Japan during the post-war decades, the associated gendering of consumer practices, and the extent to which housewives, as the holders of the purse-strings within the Japanese nuclear household, were empowered by the establishment of the breadwinner-homemaker model

Three-dimensional structure of the weakly associated protein homodimer SeR13 using RDCs and paramagnetic surface mapping

The traditional NMR-based method for determining oligomeric protein structure usually involves distinguishing and assigning intra- and intersubunit NOEs. This task becomes challenging when determining symmetric homo-dimer structures because NOE cross-peaks from a given pair of protons occur at the same position whether intra- or intersubunit in origin. While there are isotope-filtering strategies for distinguishing intra from intermolecular NOE interactions in these cases, they are laborious and often prove ineffectual in cases of weak dimers, where observation of intermolecular NOEs is rare. Here, we present an efficient procedure for weak dimer structure determination based on residual dipolar couplings (RDCs), chemical shift changes upon dilution, and paramagnetic surface perturbations. This procedure is applied to the Northeast Structural Genomics Consortium protein target, SeR13, a negatively charged Staphylococcus epidermidis dimeric protein (Kd 3.4 ± 1.4 mM) composed of 86 amino acids. A structure determination for the monomeric form using traditional NMR methods is presented, followed by a dimer structure determination using docking under orientation constraints from RDCs data, and scoring under residue pair potentials and shape-based predictions of RDCs. Validation using paramagnetic surface perturbation and chemical shift perturbation data acquired on sample dilution is also presented. The general utility of the dimer structure determination procedure and the possible relevance of SeR13 dimer formation are discussed. Published by Wiley-Blackwell. © 2010 The Protein Society

Toll-like receptor 4 is a therapeutic target for prevention and treatment of liver failure

Background and aims: Toll-like receptor 4 (TLR4) plays an essential role in mediating organ injury in acute liver failure (ALF) and acute-on-chronic liver failure (ACLF). Here we assess whether inhibiting TLR4 signaling can ameliorate liver failure and serve as a potential treatment. / Material and Methods: Circulating TLR4 ligands and hepatic TLR4 expression was measured in plasma samples and liver biopsies from patients with cirrhosis. TAK-242 (TLR4 inhibitor) was tested in vivo with 10mg/Kg, i.p. in rodent models of ACLF (bile duct ligation + lipopolysaccharide (LPS); carbontetrachloride + LPS) and ALF (Galactosamine + LPS) and in vitro on immortalized human monocytes (THP-1) and hepatocytes (HHL5).. The in vivo therapeutic effect was assessed by coma free survival, organ injury and cytokine release and in vitro by measuring IL6, IL1b or cell injury (TUNEL), respectively. / Results: In patients with cirrhosis, hepatic TLR4 expression was upregulated and circulating TLR4 ligands were increased (p<0.001). ACLF in rodents was associated with a switch from apoptotic cell death in ALF to non-apoptotic forms of cell death. TAK-242 reduced LPS induced cytokine secretion and cell death (p=0.002) in hepatocytes and monocytes in vitro. In rodent models of ACLF, TAK-242 administration improved coma free survival, reduced the degree of hepatocyte cell death in liver p<0.001) and kidneys (p=0.048) and reduced circulating cytokine levels (IL1b p<0.001). In a rodent model of ALF TAK-242 prevented organ injury (p<0.001) and systemic inflammation (IL1b p<0.001). / Conclusion: This study shows that TLR4 signaling is a key factor in the development of both ACLF and ALF and its inhibition improves severity of organ injury and outcome. TAK-242 may be of therapeutic relevance in patients with liver failure

O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis

Protein O-GlcNAcylation is an essential post-translational modification on hundreds of intracellular proteins in metazoa, catalyzed by O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) using unknown mechanisms of transfer and substrate recognition. Through crystallographic snapshots and mechanism-inspired chemical probes, we define how human OGT recognizes the sugar donor and acceptor peptide and uses a new catalytic mechanism of glycosyl transfer, involving the sugar donor α-phosphate as the catalytic base as well as an essential lysine. This mechanism seems to be a unique evolutionary solution to the spatial constraints imposed by a bulky protein acceptor substrate and explains the unexpected specificity of a recently reported metabolic OGT inhibitor. © 2012 Nature America, Inc. All rights reserved

ICAR: endoscopic skull‐base surgery

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Rethinking Japan's Lifetime Employment for the 21st Century

Japan’s practice of so-called lifetime employment is well known. This system arose out of labour unrest in the early post-war years and was the result of both collective bargaining and recognition by bureaucratic and corporate leaders that Japan needed to secure a committed workforce and a stable labour market if it was to pursue long-term economic growth. The system was constructed around three central pillars which were viewed as distinctly Japanese - implicit long-term employment for a core regular workforce, seniority-based wages and enterprise-based unions - and this focused employment strategy was heralded as a key factor driving Japan’s subsequent economic advancement. It was also founded upon a more universal concept at that time - the male breadwinner model. This meant a distinctive female employment system developed alongside the better known lifetime employment system which was predominantly applied to male employees. Japan was able to reap the social and economic benefits of a gendered division of labour that harnessed the strong commitment of a core male workforce while effectively making use of a growing non-regular female workforce as a buffer to ensure stable employment for males

Mass spectrometry assisted assignment of NMR resonances in reductively \u3csup\u3e13\u3c/sup\u3eC-methylated proteins

Reductive 13C-methylation of proteins has been used as an isotope labeling strategy to study protein structure, function, and dynamics by nuclear magnetic resonance (NMR) spectroscopy. However, assigning the resulting 13C-dimethylamine peaks in a 1H-13C NMR spectrum has proved to be difficult, but it is important to expand the scope of the method. The assignment strategy presented here utilizes mass spectrometry (MS) for sequence identification and varying 13C/12C isotope ratios to correlate with NMR data. The site-specific reactivity of the lysines and N-terminal amine of a protein is exploited to produce a sample with varying 13C/12C ratios at each dimethylamine. MS and NMR are used to quantitate and correlate these ratios in order to assign peaks in the 1H-13C NMR spectrum. Hen egg white lysozyme was used as a model protein to demonstrate this assignment strategy. Copyright © 2005 American Chemical Society