Circadian regulation of lipid mobilization in white adipose tissues.

In mammals, a network of circadian clocks regulates 24-h rhythms of behavior and physiology. Circadian disruption promotes obesity and the development of obesity-associated disorders, but it remains unclear to which extent peripheral tissue clocks contribute to this effect. To reveal the impact of the circadian timing system on lipid metabolism, blood and adipose tissue samples from wild-type, Clock Delta 19, and Bmall(-/-) circadian mutant mice were subjected to biochemical assays and gene expression profiling. We show diurnal variations in lipolysis rates and release of free fatty acids (FFAs) and glycerol into the blood correlating with rhythmic regulation of two genes encoding the lipolysis pacemaker enzymes, adipose triglyceride (TG) lipase and hormone-sensitive lipase, by self-sustained adipocyte clocks. Circadian clock mutant mice show low and nonrhythmic FFA and glycerol blood content together with decreased lipolysis rates and increased sensitivity to fasting. Instead circadian clock disruption promotes the accumulation of TGs in white adipose tissue (WAT), leading to increased adiposity and adipocyte hypertrophy. In summary, circadian modulation of lipolysis rates regulates the availability of lipid-derived energy during the day, suggesting a role for WAT clocks in the regulation of energy homeostasis

A massive, distant proto-cluster at z=2.47 caught in a phase of rapid formation?

Numerical simulations of cosmological structure formation show that the Universe's most massive clusters, and the galaxies living in those clusters, assemble rapidly at early times (2.5 < z < 4). While more than twenty proto-clusters have been observed at z > 2 based on associations of 5-40 galaxies around rare sources, the observational evidence for rapid cluster formation is weak. Here we report observations of an asymmetric, filamentary structure at z = 2.47 containing seven starbursting, submillimeter-luminous galaxies and five additional AGN within a comoving volume of 15000 Mpc$^{3}$. As the expected lifetime of both the luminous AGN and starburst phase of a galaxy is ~100 Myr, we conclude that these sources were likely triggered in rapid succession by environmental factors, or, alternatively, the duration of these cosmologically rare phenomena is much longer than prior direct measurements suggest. The stellar mass already built up in the structure is $\sim10^{12}M_{\odot}$ and we estimate that the cluster mass will exceed that of the Coma supercluster at $z \sim 0$. The filamentary structure is in line with hierarchical growth simulations which predict that the peak of cluster activity occurs rapidly at z > 2.Comment: 7 pages, 3 figures, 2 tables, accepted in ApJL (small revisions from previous version

Impaired Bioenergetics in Mutant Mitochondrial DNA Determines Cell Fate During Seizure-Like Activity

Mutations in genes affecting mitochondrial proteins are increasingly recognised in patients with epilepsy, but the factors determining cell fate during seizure activity in these mutations remain unknown. Fluorescent dye imaging techniques were applied to fibroblast cell lines from patients suffering from common mitochondrial mutations and to age-matched controls. Using live cell imaging techniques in fibroblasts, we show that fibroblasts with mutations in the mitochondrial genome had reduced mitochondrial membrane potential and NADH pools and higher redox indices, indicative of respiratory chain dysfunction. Increasing concentrations of ferutinin, a Ca2+ ionophore, led to oscillatory Ca2+ signals in fibroblasts resembling dynamic Ca2+ changes that occur during seizure-like activity. Co-monitoring of mitochondrial membrane potential (ΔΨm) changes induced by ferutinin showed accelerated membrane depolarisation and cell collapse in fibroblasts with mutations in the mitochondrial genome when compared to controls. Ca2+ flash photolysis using caged Ca2+ confirmed impaired Ca2+ handling in fibroblasts with mitochondrial mutations. Findings indicate that intracellular Ca2+ levels cannot be compensated during periods of hyperexcitability, leading to Ca2+ overload and subsequent cell death in mitochondrial diseases

An aerial parallel manipulator with shared compliance

Accessing and interacting with difficult to reach surfaces at various orientations is of interest within a variety of industrial contexts. Thus far, the predominant robotic solution to such a problem has been to leverage the maneuverability of a fully actuated, omnidirectional aerial manipulator. Such an approach, however, requires a specialised system with a high relative degree of complexity, thus reducing platform endurance and real-world applicability. The work here presents a new aerial system composed of a parallel manipulator and conventional, underactuated multirotor flying base to demonstrate interaction with vertical and non-vertical surfaces. Our solution enables compliance to external disturbance on both subsystems, the manipulator and flying base, independently with a goal of improved overall system performance when interacting with surfaces. To achieve this behaviour, an admittance control strategy is implemented on various layers of the flying base's dynamics together with torque limits imposed on the manipulator actuators. Experimental evaluations show that the proposed system is compliant to external perturbations while allowing for differing interaction behaviours as compliance parameters of each subsystem are altered. Such capabilities enable an adjustable form of dexterity in completing sensor installation, inspection and aerial physical interaction tasks. A video of our system interacting with various surfaces can be found here: https://youtu.be/38neGb8-lXg

Cosmic Microwave Background Anisotropy Measurement From Python V

We analyze observations of the microwave sky made with the Python experiment in its fifth year of operation at the Amundsen-Scott South Pole Station in Antarctica. After modeling the noise and constructing a map, we extract the cosmic signal from the data. We simultaneously estimate the angular power spectrum in eight bands ranging from large (l ~ 40) to small (l ~ 260) angular scales, with power detected in the first six bands. There is a significant rise in the power spectrum from large to smaller (l ~ 200) scales, consistent with that expected from acoustic oscillations in the early Universe. We compare this Python V map to a map made from data taken in the third year of Python. Python III observations were made at a frequency of 90 GHz and covered a subset of the region of the sky covered by Python V observations, which were made at 40 GHz. Good agreement is obtained both visually (with a filtered version of the map) and via a likelihood ratio test.Comment: 28 pages, ApJ accepted, to appear v584 n2 ApJ Feb 20, 200

Evaluating immersive teleoperation interfaces: coordinating robot radiation monitoring tasks in nuclear facilities

We present a virtual reality (VR) teleoperation interface for a ground-based robot, featuring dense 3D environment reconstruction and a low latency video stream, with which operators can immersively explore remote environments. At the UK Atomic Energy Authority's (UKAEA) Remote Applications in Challenging Environments (RACE) facility, we applied the interface in a user study where trained robotics operators completed simulated nuclear monitoring and decommissioning style tasks to compare VR and traditional teleoperation interface designs. We found that operators in the VR condition took longer to complete the experiment, had reduced collisions, and rated the generated 3D map with higher importance when compared to non-VR operators. Additional physiological data suggested that VR operators had a lower objective cognitive workload during the experiment but also experienced increased physical demand. Overall the presented results show that VR interfaces may benefit work patterns in teleoperation tasks within the nuclear industry, but further work is needed to investigate how such interfaces can be integrated into real world decommissioning workflows

Kolmogorov cosmic microwave background sky

A new map of the sky representing the degree of randomness in the cosmic microwave background (CMB) temperature has been obtained. The map based on estimation of the Kolmogorov stochasticity parameter clearly distinguishes the contribution of the Galactic disk from the CMB and reveals regions of various degrees of randomness that can reflect the properties of inhomogeneities in the Universe. For example, among the high randomness regions is the southern non-Gaussian anomaly, the Cold Spot, with a stratification expected for the voids. Existence of its counterpart, a Northern Cold Spot with almost identical randomness properties among other low-temperature regions is revealed. By its informative power, Kolmogorov's map can be complementary to the CMB temperature and polarization sky maps.Comment: A & A (in press), to match the published version, 4 pages, 5 figs, 2 Table

Large scale structure around a z=2.1 cluster

The most prodigious starburst galaxies are absent in massive galaxy clusters today, but their connection with large scale environments is less clear at $z\gtrsim2$. We present a search of large scale structure around a galaxy cluster core at $z=2.095$ using a set of spectroscopically confirmed galaxies. We find that both color-selected star-forming galaxies (SFGs) and dusty star-forming galaxies (DSFGs) show significant overdensities around the $z=2.095$ cluster. A total of 8 DSFGs (including 3 X-ray luminous active galactic nuclei, AGNs) and 34 SFGs are found within a 10 arcmin radius (corresponds to $\sim$15 cMpc at $z\sim2.1$) from the cluster center and within a redshift range of $\Delta z=0.02$, which leads to galaxy overdensities of $\delta_{\rm DSFG}\sim12.3$ and $\delta_{\rm SFG}\sim2.8$. The cluster core and the extended DSFG- and SFG-rich structure together demonstrate an active cluster formation phase, in which the cluster is accreting a significant amount of material from large scale structure while the more mature core may begin to virialize. Our finding of this DSFG-rich structure, along with a number of other protoclusters with excess DSFGs and AGNs found to date, suggest that the overdensities of these rare sources indeed trace significant mass overdensities. However, it remains puzzling how these intense star formers are triggered concurrently. Although an increased probability of galaxy interactions and/or enhanced gas supply can trigger the excess of DSFGs, our stacking analysis based on 850 $\mu$m images and morphological analysis based on rest-frame optical imaging do not show such enhancements of merger fraction and gas content in this structure.Comment: 11 pages, 4 figures, ApJ accepte

A Millimeter-wave Galactic Plane Survey with the BICEP Polarimeter

In order to study inflationary cosmology and the Milky Way Galaxy's composition and magnetic field structure, Stokes I, Q, and U maps of the Galactic plane covering the Galactic longitude range 260° < ℓ < 340° in three atmospheric transmission windows centered on 100, 150, and 220 GHz are presented. The maps sample an optical depth 1 ≾ AV ≾ 30, and are consistent with previous characterizations of the Galactic millimeter-wave frequency spectrum and the large-scale magnetic field structure permeating the interstellar medium. The polarization angles in all three bands are generally perpendicular to those measured by starlight polarimetry as expected and show changes in the structure of the Galactic magnetic field on the scale of 60°. The frequency spectrum of degree-scale Galactic emission is plotted between 23 and 220 GHz (including WMAP data) and is fit to a two-component (synchrotron and dust) model showing that the higher frequency BICEP data are necessary to tightly constrain the amplitude and spectral index of Galactic dust. Polarized emission is detected over the entire region within two degrees of the Galactic plane, indicating the large-scale magnetic field is oriented parallel to the plane of the Galaxy. A trend of decreasing polarization fraction with increasing total intensity is observed, ruling out the simplest model of a constant Galactic magnetic field orientation along the line of sight in the Galactic plane. A generally increasing trend of polarization fraction with electromagnetic frequency is found, varying from 0.5%-1.5% at frequencies below 50 GHz to 2.5%-3.5% above 90 GHz. The effort to extend the capabilities of BICEP by installing 220 GHz band hardware is described along with analysis of the new band