Prevention of acute ammonia toxicity in beluga, Huso huso, using natural zeolite

This study was accomplished to examine the efficiency of natural zeolite in preventing acute toxicity of total ammonia to Huso huso. The study was performed using Water Static Method in 96 hours. Fish averaged 46 ± 5 g in weight and 22 ± 4 cm in total length were exposed to four different concentrations (15, 30, 50, 75 mgL-1) of ammonia and a group was considered as control. Under stable condition, the lethal concentration of ionized ammonia was 75 mgL-1 in 96 hours. In the lethal concentration of total ammonia, different amounts of 5, 10, 15 g.L-1 granulated clinoptilolite zeolite were used. Results indicated significant differences between treatments and control (p<0.05). By increasing Clinoptilolite zeolite in each treatment, the survival rate of fish also increased significantly (p<0.05). In lethal concentration of ammonia, the use of 15 g.L-1 zeolite could prevent the mortality rate. Histopathological findings showed that major lesions in gill filaments included hemorrhage, hyperemia, hyperplasia, epithelial cells necrosis. There were hemorrhage, hyperemia, degenerated tubules of kidney, expansion of Bowman's capsule in kidney and hepatocytes necrosis in liver

Use of Bacillus probiotics for immune responses and intestinal microflora of white leg shrimp Litopenaeus vannamei (Boone, 1931) post larvae

The effect of dietary containing of Bacillus subtilis and Bacillus licheniformis in three groups including commercial, commercial-indigenous and indigenous was investigated on the immune parameters (glucose, albumin, total protein, lysozyme, cortisol, immunoglobulin M (IgM)) and the intestinal flora of white leg shrimp (Litopenaeus vannamei) post larvae. The shrimp were fed for 60 days with four different diets: control (without probiotics), diet T1 supplemented with 1.5×106 CFU g-1 commercial probiotic, diet T2 with 1.5×106 CFU g-1 commercial-indigenous probiotic, diet T3 with 1.5×106 CFU g-1 indigenous probiotic. At the end of experimental period, the levels of biochemical parameters (glucose, total protein, lysozyme, cortisol, IgM) of shrimp fed probiotic diets were significantly higher than in those shrimps fed the control diet for 60 days. However, albumin concentrations showed no significant difference between the experimental treatments and the control, but increased by 1.19, 1.15 and 1.14 after 60 days of feeding with diets T1, T2 and T3, respectively. Likewise, population density of Bacillus bacteria counted in digestive tract of shrimps treated with probiotic were significantly higher than the control group. Results of this study indicated that the addition of probiotic bacilli can improve immune parameters and modulates intestinal microbiota of shrimp (L. vannamei) post larvae

High-Throughput Time Series Metabolomic Analysis of a Systematically Perturbed Plant System

In the post-genomic era, availability of high-throughput profiling techniques enabled the measurement of entire cellular molecular fingerprints. Major characteristics of the high-throughput revolution were that (a) studying biological problems did not have to rely on prior hypotheses, while (b) parallel occurring phenomena, previously assumed disconnected, could now be simultaneously observed. Metabolomics is the newest of the "omics" techniques. It enables the quantification of hundreds of free metabolite pools, providing a metabolic fingerprint. Considering the importance of cellular metabolism, which is the net effect of changes at the genomic, transcriptomic and proteomic levels and of the cell with its environment, the metabolomic profile, is a fundamental determinant of cellular physiology. Obtaining accurate and complete metabolomic profiles is thus of great importance. However, being recent technology, metabolomics is currently at its standardization phase. As part of my PhD thesis research, I focused on addressing several current challenges in metabolomics technology development. Specifically a novel data correction, validation and normalization strategy for gas chromatography-mass spectrometry (GC-MS) metabolomic profiling analysis was developed, which dramatically increased the accuracy and reliability of GC-MS metabolomic profiles. The optimized metabolomics protocol was applied to study the short-term dynamic response of systematically perturbed Arabidopsis thaliana liquid culture system to study regulation of its primary metabolism. The biological system was studied under conditions of elevated CO2 stress, salt (NaCl) stress, sugar (trehalose) signal, and hormone (ethylene) signal, applied individually; the latter three stresses also applied in combination with the CO2 stress. Analysis of the obtained results required the appropriate application of multivariate statistical analysis techniques, which are developed mainly in transcriptomic analysis, into metabolomics analysis for the first time. The acquired results identified important new regulatory information about the biological systems resulting in new targets for metabolic engineering of plants. The large number of dynamic perturbation allowed re-construction of metabolic networks to identify possible novel metabolic pathways based on correlations between metabolic profiles. In addition, it demonstrates the advantages of dynamic, multiple-stress "omic" analysis for the elucidation of plant systems function. In this sense, it contributes in further advancing the computational and experimental metabolic engineering and systems biology toolbox

Investigation of horizontal coherent structures in a shallow open-channel flow using velocity signal decomposition

River morphodynamics and sediment transportRiver morphology and morphodynamic

Effect of copper sulphate on behavioral and histopathological changes in roach, Rutilus rutilus caspicus

The research was accomplished in order to study behavioral and histopathological effects of Copper on Rutilus rutilus caspicus under experimental condition. The study was performed using Water Static Method during 96 hours. 15 fish with averaged weight 2±0.5 g in weight were encountered to different concentrations (0, 0.1, 0.2, 0.3, 0.4 mg/l) of Copper. A group of fish was considered as control. Under stable condition and aeration, the lethal concentration was 0.4 mg/l. Results indicated that there was significant differences between treatments when copper concentration increased (p<0.05). It was found that in the high concentration of Copper nominal signs of toxicity such as convulsion, air gulping and flared operculum were observed. Histopathological sings were hyperplasia, edema, hyperemia, hemorrhage, expansion of Bowman’s capsule and hepatocytes necrosis. In control group no lesion was observed

The Role of Green Activity-Based Costing in Achieving Sustainability Development: Evidence From Iraq

Purpose: This study aimed to assess the effects of applying green activity-based costing (GABC) on the Sustainability Development of companies.   Theoretical framework: The theoretical framework included highlight on environmental issues, and the role of Modern methods of Cost and managerial accounting in reducing the harmful effects of industrial waste.   Design/methodology/approach: there are variety of reasons to carry out the study; including regulatory compliance, sustainable consumption, public perception, potential competitive advantages, and the impact of Sustainability Development on economic performance, stakeholders are urging or requiring organizations to be more ecologically conscious with regard to their products and operations. In order to accomplish the study's goals, it was implemented at the Basra Oil Refinery and based on actual data from the study sample's activity. method was relied upon distribution of costs based on green activity, the identification of its effects on the facility's Sustainability Development, and the creation of a model for the use of GABC.   Findings: According to the results of the experimental study, the study's findings that GABC may be used to assign expenditures based on green activity and to show how it affects both economic and Sustainability Development.   Research, Practical &amp; Social implications: Because all manufacturing and consumption activities have some sort of negative impact on the environment during the creation, use, and disposal phases of their respective value chains, environmental management issues have recently been linked to the economy.   Originality/value: The research value of our current study, by employ modern methods of commissioning accounting in Iraqi manufacturing companies

Detecting Sticky Costs in Iraqi Industrial Companies an Empirical Study of a Sample of Iraqi Companies Registered on Iraq Stock Exchange

Purpose: This research aims to reveal the sticky costs in Iraqi joint stock companies.   Theoretical framework: Sticky costs refer to the costs that increase with the increase in revenues by a greater amount than their decrease in exchange for a similar decrease in revenues. To manage cost, it is important to understand and know the behavior of cost.   Design/methodology/approach: This research uses inductive and descriptive analytical methods that is commonly used in applied and field studies on accounting and management issues, as well as in literature and empirical studies to verify research proposals. This study adopted multiple logarithmic linear regression analysis estimated by ordinary least squares (OLS) to examine asymmetrical behavior of selling costs, general and administrative costs, and costs of goods sold individually and collectively (total costs) for a sample (101) of published lists of Iraqi joint stock companies registered in Iraqi Stock Exchange for the period 2010-2019.   Findings: The results showed that sticky behavior of selling and general and administrative costs and their components is a common phenomenon in cost behavior of Iraqi joint stock companies during the study period, while costs of goods sold, and total costs are not. The latter may have been showing anti-sticky behavior.   Research, Practical &amp; Social implications: Our work contrasts the conventional model of cost behaviour, in which costs move proportionately with changes in activity, with an alternative model, in which sticky costs emerge. This is because managers consciously shift the resources devoted to activities.   Originality/value: The sticky cost research that has been done in relation to the initial sticky cost research, particularly in industrialised nations like the UAS, Uk, and Canada, is the main emphasis of this study. Future research will take into account how sticky research is developing in any other nations, which could expand our understanding of sticky cost research as a whole

Anesthetic effect of tricaine methanesulfonate, clove oil and electroanesthesia on lysozyme activity of Oncorhynchus mykiss

There is a few available information about the effect of anesthetics, specially electroanesthesia on immune parameters in fish. In the present work, two anesthetics, MS222 (50 ppm), clove oil (25 ppm), and electroanesthesia were tested in rainbow trout (Oncorhynchus mykiss) in narcosis stage. The results showed, clove oil and electroanesthesia increase the lysozyme activity 24 h after anesthesia(p0.05). It seems use of MS222 during aquacultural practices may induce lesser effect in rainbow trout

Importance of radiative transfer processes in urban climate models: A study based on the PALM 6.0 model system

Including radiative transfer processes within the urban canopy layer into microscale urban climate models (UCMs) is essential to obtain realistic model results. These processes include the interaction of buildings and vegetation with shortwave and longwave radiation, thermal emission, and radiation reflections. They contribute differently to the radiation budget of urban surfaces. Each process requires different computational resources and physical data for the urban elements. This study investigates how much detail modellers should include to parameterize radiative transfer in microscale building-resolving UCMs. To that end, we introduce a stepwise parameterization method to the Parallelized Large-eddy Simulation Model (PALM) system 6.0 to quantify individually the effects of the main radiative transfer processes on the radiation budget and on the flow field. We quantify numerical simulations of both simple and realistic urban configurations to identify the major and the minor effects of radiative transfer processes on the radiation budget. The study shows that processes such as surface and vegetation interaction with shortwave and longwave radiation will have major effects, while a process such as multiple reflections will have minor effects. The study also shows that radiative transfer processes within the canopy layer implicitly affect the incoming radiation since the radiative transfer model is coupled to the radiation model. The flow field changes considerably in response to the radiative transfer processes included in the model. The study identified those processes which are essentially needed to assure acceptable quality of the flow field. These processes are receiving radiation from atmosphere based on the sky-view factors, interaction of urban vegetation with radiation, radiative transfer among urban surfaces, and considering at least single reflection of radiation. Omitting any of these processes may lead to high uncertainties in the model results.publishedVersio