Comparative Study of Different Memetic Algorithm Configurations for the Cyclic Bandwidth Sum Problem

The Cyclic Bandwidth Sum Problem (CBSP) is an NP-Hard Graph Embedding Problem which aims to embed a simple, finite graph (the guest) into a cycle graph of the same order (the host) while minimizing the sum of cyclic distances in the host between guest’s adjacent nodes. This paper presents preliminary results of our research on the design of a Memetic Algorithm (MA) able to solve the CBSP. A total of 24 MA versions, induced by all possible combinations of four selection schemes, two operators for recombination and three for mutation, were tested over a set of 25 representative graphs. Results compared with respect to the state-of-the-art top algorithm showed that all the tested MA versions were able to consistently improve its results and give us some insights on the suitability of the tested operators

E3 Ligase Activity of XIAP RING Domain Is Required for XIAP-Mediated Cancer Cell Migration, but Not for Its RhoGDI Binding Activity

Although an increased expression level of XIAP is associated with cancer cell metastasis, the underlying molecular mechanisms remain largely unexplored. To verify the specific structural basis of XIAP for regulation of cancer cell migration, we introduced different XIAP domains into XIAP−/− HCT116 cells, and found that reconstitutive expression of full length HA-XIAP and HA-XIAP ΔBIR, both of which have intact RING domain, restored β-Actin expression, actin polymerization and cancer cell motility. Whereas introduction of HA-XIAP ΔRING or H467A mutant, which abolished its E3 ligase function, did not show obvious restoration, demonstrating that E3 ligase activity of XIAP RING domain played a crucial role of XIAP in regulation of cancer cell motility. Moreover, RING domain rather than BIR domain was required for interaction with RhoGDI independent on its E3 ligase activity. To sum up, our present studies found that role of XIAP in regulating cellular motility was uncoupled from its caspase-inhibitory properties, but related to physical interaction between RhoGDI and its RING domain. Although E3 ligase activity of RING domain contributed to cell migration, it was not involved in RhoGDI binding nor its ubiquitinational modification

A Conserved Mechanism for Control of Human and Mouse Embryonic Stem Cell Pluripotency and Differentiation by Shp2 Tyrosine Phosphatase

Recent studies have suggested distinctive biological properties and signaling mechanisms between human and mouse embryonic stem cells (hESCs and mESCs). Herein we report that Shp2, a protein tyrosine phosphatase with two SH2 domains, has a conserved role in orchestration of intracellular signaling cascades resulting in initiation of differentiation in both hESCs and mESCs. Homozygous deletion of Shp2 in mESCs inhibited differentiation into all three germ layers, and siRNA-mediated knockdown of Shp2 expression in hESCs led to a similar phenotype of impaired differentiation. A small molecule inhibitor of Shp2 enzyme suppressed both hESC and mESC differentiation capacity. Shp2 modulates Erk, Stat3 and Smad pathways in ES cells and, in particular, Shp2 regulates BMP4-Smad pathway bi-directionally in mESCs and hESCs. These results reveal a common signaling mechanism shared by human and mouse ESCs via Shp2 modulation of overlapping and divergent pathways

Spatially Weighted Estimation of Broadacre Crop Growth Improves Gap-Filling of Landsat NDVI

Seasonal climate is the main driver of crop growth and yield in broadacre grain cropping systems. With a 40-year record of 30 m resolution images and 16-day revisits, the Landsat satellite series is ideal for producing long-term records of remotely sensed phenology to build understanding of how climate affects crop growth. However, the time-series of Landsat images exhibits gaps caused by cloud cover, which is common in wet periods when crops reach maximum growth. We propose a novel spatial–temporal approach to gap-filling that avoids data fusion. Crop growth curve estimation is used to perform temporal smoothing and incorporation of spatial weights allows spatial smoothing. We tested our approach using Landsat NDVI data acquired for an 8000 ha study area in Western Australia using a train/test approach where 157 available Landsat-7 images between 2013 and 2019 were used to train the model, and 95 at least 80% cloud-free Landsat-8 images from the same period were used to test its performance. We found that compared to nonspatial estimation, use of spatial weights in growth curve estimation improved correlation between observed and predicted NDVI by 75%, MAE by 31% and RMSE by 75%. For cropland, the correlation is improved by 58%, the MAE by 36% and the RMSE by 76%. We conclude that spatially weighted estimation of crop growth curves can be used to fill spatial and temporal gaps in Landsat NDVI for the purpose of within-field monitoring. Our approach is also applicable to other data sources and vegetation indices

Speciation and contamination assessment of metals in the sediments from the Lanzhou section of the Yellow River, China

Heavy metals such as Cu, Fe, Mn, Ni, Zn, Cd, Cr and Pb in surface sediments from the Lanzhou Section of the Yellow River were investigated to analyze chemical speciation by a sequential "extraction and to evaluate their pollution level. The metal contents in the sediments were found to be in the range of (mg/kg) Cu 15.52-57.50, Fe 19 600.33-48 350.00, Mn 493.50-698.93, Ni 9.34-53.20, Zn 64.86-168.57, Cd 1.09-4.25, Cr 70.00-139.33, Pb 5.66-19.13. The arrangement of metal contents from higher to lower was: Fe > Mn > Cr > Zn > Ni > Cu > Pb > Cd. Fe, Mn and Cu and could have the same source according to their correlations. The quantity of immobile speciation (residual) was observed to be higher when compared with mobile speciation (acid soluble, reducible, and oxidizable) of Fe, Cu, Cd and Cr. The highly enrichment factors of Ni and Cr created a high environmental risk. The order of contamination level for heavy metals in sediment was Cr > Cd > Pb > Cu > Mn > Ni > Fe > Zn on the basis of performance of the geoaccumulation index, enrichment factor and pollution load index

Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2

The Src-homology 2 (SH2) domain-containing tyrosine phosphatase Shp2 has been known to regulate various signaling pathways triggered by receptor and cytoplasmic tyrosine kinases. Here we describe a novel function of Shp2 in control of lipid metabolism by mediating degradation of fatty acid synthase (FASN). p38-phosphorylated COP1 accumulates in the cytoplasm and subsequently binds FASN through Shp2 here as an adapter, leading to FASN-Shp2-COP1 complex formation and FASN degradation mediated by ubiquitination pathway. By fasting p38 is activated and stimulates FASN protein degradation in mice. Consistently, the FASN protein levels are dramatically elevated in mouse liver and pancreas in which Shp2/Ptpn11 is selectively deleted. Thus, this study identifies a new activity for Shp2 in lipid metabolism

Land subsidence caused by internal soil erosion owing to pumping confined aquifer groundwater during the deep foundation construction in Shanghai

Land subsidence is presented in many factors in different areas with urbanization. Internal soil erosion, owing to pumping confined groundwater during the deep foundation pit construction, has contributed to land subsidence. Four governing equations are presented to describe the process of internal soil erosion based on the mathematical-physical model. The finite element computation results, based on practical deep foundation pit engineering consisted of 8 layers of soil of Shanghai area, demonstrate that internal soil erosion will cause the increment of land subsidence and deformation and is related to the hydraulic gradient and the characters of the soils

Transformation from Graphs to Signals and Back

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