A modified priority-based encoding for design of a closed-loop supply chain network using a discrete league championship algorithm

In a closed-loop supply chain network, the aim is to ensure a smooth fow of materials and attaining the maximum value from returning and end-of-life goods. Tis paper presents a single-objective deterministic mixed integer linear programming (MILP) model for the closed-loop supply chain (CLSC) network design problem consisting of plants, collection centers, disposal centers, and customer zones. Our model minimizes the total costs comprising fxed opening cost of plants, collection, disposal centers, and transportation costs of products among the nodes. As supply chain network design problems belong to the class of NP-hard problems, a novel league championship algorithm (LCA) with a modifed priority-based encoding is applied to fnd a near-optimal solution. New operators are defned for the LCA to search the discrete space. Numerical comparison of our proposed encoding with the existing approaches in the literature is indicative of the high quality performance of the proposed encoding

Yield, fruit quality and physiological responses of melon cv. Khatooni under deficit irrigation

To evaluate the effect of water deficit stress on growth, yield, fruit quality and physiological traits of melon cv. Khatooni, field experiments were conducted in split plot randomized complete block design with three replications. In 2014, irrigation treatments consisted of two deficit irrigation regimes, 33% and 66% of ETc (crop evapotranspiration), and 100% ETc as the control (DI33, DI66 and I100). In 2015, irrigation treatments applied were: 40, 70 and 100% ETc (DI40, DI70 and I100). The results showed that plant height and leaf area decreased from treatment I100 to DI40 and DI33. The highest average fruit weigh and yield were obtained from irrigation 100% ETc for both years. The water use efficiency (WUE) significantly increased in response to increase water deficit stress. Deficit irrigation treatments significantly decreased leaf relative water content, vitamin C and fruit firmness, whereas antioxidant enzymes activity, proline and total soluble solid contents increased. These results suggest that the crop is sensitive to water deficits, that moderate water stress (DI70 and DI66) reduced yield by about 28.5-38.2% and severe water stress (DI40 and DI33) had a much more marked effect, reducing yield by 48.1-61.4%

Exosomes derived from human mesenchymal stem cells preserve mouse islet survival and insulin secretion function

Islet cell death and loss of function after isolation and before transplantation is considered a key barrier to successful islet transplantation outcomes. Mesenchymal stem cells (MSCs) have been used to protect isolated islets owing to their paracrine potential partially through the secretion of vascular endothelial growth factor (VEGF). The paracrine functions of MSCs are also mediated, at least in part, by the release of extracellular vesicles including exosomes. In the present study, we examined (i) the effect of exosomes from human MSCs on the survival and function of isolated mouse islets and (ii) whether exosomes contain VEGF and the potential impact of exosomal VEGF on the survival of mouse islets. Isolated mouse islets were cultured for three days with MSC-derived exosomes (MSC-Exo), MSCs, or MSC-conditioned media without exosomes (MSC-CM-without-Exo). We investigated the effects of the exosomes, MSCs, and conditioned media on islet viability, apoptosis and function. Besides the expression of apoptotic and pro-survival genes, the production of human and mouse VEGF proteins was evaluated. The MSCs and MSC-Exo, but not the MSC-CM-without-Exo, significantly decreased the percentage of apoptotic cells and increased islet viability following the downregulation of pro-apoptotic genes and the upregulation of pro-survival factors, as well as the promotion of insulin secretion. Human VEGF was observed in the isolated exosomes, and the gene expression and protein production of mouse VEGF significantly increased in islets cultured with MSC-Exo. MSC-derived exosomes are as efficient as parent MSCs for mitigating cell death and improving islet survival and function. This cytoprotective effect was probably mediated by VEGF transfer, suggesting a pivotal strategy for ameliorating islet transplantation outcomes

A comparison of maladaptive early schemas and appearance schemas in obese and normal weight control subjects

The purpose of this study was to compare early maladaptive and appearance schemas in obse and and normal-weight subjects. Materials and Methods: The method of the study was causal- comparative and groups were included 30 obese (BMI�35) and 30 normal-weight adults (BMI<25). All participants completed Young Schema Questionnaire�Short Version (YSQ-S) and appearance schema Inventory (ASI) questionnaire. Results: Obse subjects showed significantly higher scores in compare to control group in self-sacrifice and emotional inhibition schemas. In addition, severity of appearance schemas in body- image vulnerability and self- investment subscales were significantly greater in obese subjects than in control group. Conclusion: The results of this study suggest that some early maladaptive and appearance schemas are associated with obesity and therefore, theoretical conceptualizations and psychological interventions should address the above thesis constructs. © 2015, Semnan University of Medical Sciences. All rights reserved

Vitamin D supplementation and incident dementia: Effects of sex, APOE, and baseline cognitive status

Supporting Information is available online at: https://alz-journals.onlinelibrary.wiley.com/doi/10.1002/dad2.12404#support-information-section .Copyright © 2023 The Authors. Introduction: Despite the association of vitamin D deficiency with incident dementia, the role of supplementation is unclear. We prospectively explored associations between vitamin D supplementation and incident dementia in 12,388 dementia-free persons from the National Alzheimer's Coordinating Center. Methods: Baseline exposure to vitamin D was considered D+; no exposure prior to dementia onset was considered D−. Kaplan–Meier curves compared dementia-free survival between groups. Cox models assessed dementia incidence rates across groups, adjusted for age, sex, education, race, cognitive diagnosis, depression, and apolipoprotein E (APOE) ε4. Sensitivity analyses examined incidence rates for each vitamin D formulation. Potential interactions between exposure and model covariates were explored. Results: Across all formulations, vitamin D exposure was associated with significantly longer dementia-free survival and lower dementia incidence rate than no exposure (hazard ratio = 0.60, 95% confidence interval: 0.55–0.65). The effect of vitamin D on incidence rate differed significantly across the strata of sex, cognitive status, and APOE ε4 status. Discussion: Vitamin D may be a potential agent for dementia prevention. Highlights: * In a prospective cohort study, we assessed effects of Vitamin D on dementia incidence in 12,388 participants from the National Alzheimer's Coordinating Center dataset. * Vitamin D exposure was associated with 40% lower dementia incidence versus no exposure. * Vitamin D effects were significantly greater in females versus males and in normal cognition versus mild cognitive impairment. * Vitamin D effects were significantly greater in apolipoprotein E ε4 non-carriers versus carriers. * Vitamin D has potential for dementia prevention, especially in the high-risk strata.The NACC database is funded by NIA/NIH grant U24-AG072122. NACC data are contributed by the NIA-funded ADRCs: P30-AG019610 (PI Eric Reiman, MD), P30-AG013846 (PI Neil Kowall, MD), P50-AG008702 (PI Scott Small, MD), P50-AG025688 (PI Allan Levey, MD, PhD), P50-AG047266 (PI Todd Golde, MD, PhD), P30-AG010133 (PI Andrew Saykin, PsyD), P50-AG005146 (PI Marilyn Albert, PhD), P50-AG005134 (PI Bradley Hyman, MD, PhD), P50-AG016574 (PI Ronald Petersen, MD, PhD), P50-AG005138 (PI Mary Sano, PhD), P30-AG008051 (PI Thomas Wisniewski, MD), P30-AG013854 (PI Robert Vassar, PhD), P30-AG008017 (PI Jeffrey Kaye, MD), P30AG010161 (PI David Bennett, MD), P50 AG047-366 (PI Victor Henderson, MD, MS), P30-AG010129 (PI Charles DeCarli, MD), P50-AG016573 (PI Frank LaFerla, PhD), P50-AG005131 (PI James Brewer, MD, PhD), P50-AG023501 (PI Bruce Miller, MD), P30-AG035982 (PI Russell Swerdlow, MD), P30-AG028383 (PI Linda Van Eldik, PhD), P30-AG053760 (PI Henry Paulson, MD, PhD), P30-AG010124 (PI John Trojanowski, MD, PhD), P50-AG005133 (PI Oscar Lopez, MD), P50-AG005142 (PI Helena Chui, MD), P30-AG012300 (PI Roger Rosenberg, MD), P30-AG049638 (PI Suzanne Craft, PhD), P50-AG005136 (PI Thomas Grabowski, MD), P50-AG033514 (PI Sanjay Asthana, MD, FRCP), P50-AG005681 (PI John Morris, MD), P50-AG047270 (PI Stephen Strittmatter, MD, PhD). Zahinoor Ismail is by the Canadian Institutes of Health Research (BCA2633). Maryam Ghahremani is supported by the Mathison Centre Postdoctoral Fellowship in Mental Health at the University of Calgary, Canada. This study was supported by the National Institute for Health and Care Research Exeter Biomedical Research Centre

Stable Knockdown of Adenosine Kinase by Lentiviral Anti-ADK miR-shRNAs in Wharton�s Jelly Stem Cells

Objective: In this study, we describe an efficient approach for stable knockdown of adenosine kinase (ADK) using lentiviral system, in an astrocytoma cell line and in human Wharton�s jelly mesenchymal stem cells (hWJMSCs). These sources of stem cells besides having multilineage differentiation potential and immunomodulatory activities, are easily available in unlimited numbers, do not raise ethical concerns and are attractive for gene manipulation and cell-based gene therapy. Materials and Methods: In this experimental study, we targeted adenosine kinase mRNA at 3' and performed coding sequences using eight miR-based expressing cassettes of anti-ADK short hairpin RNA (shRNAs). First, these cassettes with scrambled control sequences were cloned into expressing lentiviral pGIPZ vector. Quantitative real time-polymerase chain reaction (qRT-PCR) was used to screen multi-cassettes anti-ADK miR-shRNAs in stably transduced U-251 MG cell line and measuring ADK gene expression at mRNA level. Extracted WJMSCs were characterized using flow cytometry for expressing mesenchymal specific marker (CD44+) and lack of expression of hematopoietic lineage marker (CD45-). Then, the lentiviral vector that expressed the most efficient anti-ADK miR-shRNA, was employed to stably transduce WJMSCs. Results: Transfection of anti-ADK miR-shRNAs in HEK293T cells using CaPO4 method showed high efficiency. We successfully transduced U-251 cell line by recombinant lentiviruses and screened eight cassettes of anti-ADK miR-shRNAs in stably transduced U-251 MG cell line by qRT-PCR. RNAi-mediated down-regulation of ADK by lentiviral system indicated up to 95 down-regulation of ADK. Following lentiviral transduction of WJMSCs with anti-ADK miR-shRNA expression cassette, we also implicated, down-regulation of ADK up to 95 by qRT-PCR and confirmed it by western blot analysis at the protein level. Conclusion: Our findings indicate efficient usage of shRNA cassette for ADK knockdown. Engineered WJMSCs with genome editing methods like CRISPR/cas9 or more safe viral systems such as adeno-associated vectors (AAV) might be an attractive source in cell-based gene therapy and may have therapeutic potential for epilepsy

Stable Knockdown of Adenosine Kinase by Lentiviral Anti-ADK miR-shRNAs in Wharton�s Jelly Stem Cells

Objective: In this study, we describe an efficient approach for stable knockdown of adenosine kinase (ADK) using lentiviral system, in an astrocytoma cell line and in human Wharton�s jelly mesenchymal stem cells (hWJMSCs). These sources of stem cells besides having multilineage differentiation potential and immunomodulatory activities, are easily available in unlimited numbers, do not raise ethical concerns and are attractive for gene manipulation and cell-based gene therapy. Materials and Methods: In this experimental study, we targeted adenosine kinase mRNA at 3' and performed coding sequences using eight miR-based expressing cassettes of anti-ADK short hairpin RNA (shRNAs). First, these cassettes with scrambled control sequences were cloned into expressing lentiviral pGIPZ vector. Quantitative real time-polymerase chain reaction (qRT-PCR) was used to screen multi-cassettes anti-ADK miR-shRNAs in stably transduced U-251 MG cell line and measuring ADK gene expression at mRNA level. Extracted WJMSCs were characterized using flow cytometry for expressing mesenchymal specific marker (CD44+) and lack of expression of hematopoietic lineage marker (CD45-). Then, the lentiviral vector that expressed the most efficient anti-ADK miR-shRNA, was employed to stably transduce WJMSCs. Results: Transfection of anti-ADK miR-shRNAs in HEK293T cells using CaPO4 method showed high efficiency. We successfully transduced U-251 cell line by recombinant lentiviruses and screened eight cassettes of anti-ADK miR-shRNAs in stably transduced U-251 MG cell line by qRT-PCR. RNAi-mediated down-regulation of ADK by lentiviral system indicated up to 95 down-regulation of ADK. Following lentiviral transduction of WJMSCs with anti-ADK miR-shRNA expression cassette, we also implicated, down-regulation of ADK up to 95 by qRT-PCR and confirmed it by western blot analysis at the protein level. Conclusion: Our findings indicate efficient usage of shRNA cassette for ADK knockdown. Engineered WJMSCs with genome editing methods like CRISPR/cas9 or more safe viral systems such as adeno-associated vectors (AAV) might be an attractive source in cell-based gene therapy and may have therapeutic potential for epilepsy