An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression

On Steering Swarms

The main contribution of this paper is a novel method allowing an external observer/controller to steer and guide swarms of identical and indistinguishable agents, in spite of the agents' lack of information on absolute location and orientation. Importantly, this is done via simple global broadcast signals, based on the observed average swarm location, with no need to send control signals to any specific agent in the swarm

FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer

Fourier transform infrared (FTIR) spectroscopy is a vibrational spectroscopic technique that uses infrared radiation to vibrate molecular bonds within the sample that absorbs it. As different samples contain different molecular bonds or different configurations of molecular bonds, FTIR allows us to obtain chemical information on molecules within the sample. Fourier transform infrared microspectroscopy in conjunction with a principal component-discriminant function analysis (PC-DFA) algorithm was applied to the grading of prostate cancer (CaP) tissue specimens. The PC-DFA algorithm is used alongside the established diagnostic measures of Gleason grading and the tumour/node/metastasis system. Principal component-discriminant function analysis improved the sensitivity and specificity of a three-band Gleason score criterion diagnosis previously reported by attaining an overall sensitivity of 92.3% and specificity of 99.4%. For the first time, we present the use of a two-band criterion showing an association of FTIR-based spectral characteristics with clinically aggressive behaviour in CaP manifest as local and/or distal spread. This paper shows the potential for the use of spectroscopic analysis for the evaluation of the biopotential of CaP in an accurate and reproducible manner

Survei pengaruh ketinggian genangan Air pada Tanaman Padi di Daerah Bandung Timur

Survey Influence Of Water Inundation Rice Plant On Production Results In East Bandung Rice is a plant that can grow well in stagnant conditions. However, inundation conditions above Normal will also affect the condition of the rice crop itself, especially the production of rice Produced. Differences in time and duration Inundation will have different effects on paddy rice Growth. The height and duration of flooding substantially affect the growth of rice crops. Survey of water puddle on rice crop Against done in East Bandung City, West Java. The method For writing this journal is by conducting survey and observation to the location located at Jl. Soekarno-Hatta, Cimencrang, Bandung. by Measuring the height of a puddle from the ground Surface to the water level using the Ruler. Measurements were made randomly Based on Existing rice fields There were six samples. Reference to this journal is derived from various Scientific journals And articles relating to the topic of the journal. The results show the height of Puddles in rice plants ranging from -2 cm to 2cm. literature study Indicates height The optimum Puddle for rice plants is -2.5cm to 7.5cm

Local randomness in Hardy's correlations: Implications from information causality principle

Study of nonlocal correlations in term of Hardy's argument has been quite popular in quantum mechanics. Recently Hardy's argument of non-locality has been studied in the context of generalized non-signaling theory as well as theory respecting information causality. Information causality condition significantly reduces the success probability for Hardy's argument when compared to the result based on non-signaling condition. Here motivated by the fact that maximally entangled state in quantum mechanics does not exhibit Hardy's non-local correlation, we do a qualitative study of the property of local randomness of measured observable on each side reproducing Hardy's non-locality correlation,in the context of information causality condition. On applying the necessary condition for respecting the principle of information causality, we find that there are severe restrictions on the local randomness of measured observable in contrast to results obtained from no-signaling condition.Still, there are some restrictions imposed by quantum mechanics that are not obtained from information causality condition.Comment: 6 pages, 2 tables, new references adde

Classical communication and non-classical fidelity of quantum teleportation

In quantum teleportation, the role of entanglement has been much discussed. It is known that entanglement is necessary for achieving non-classical teleportation fidelity. Here we focus on the amount of classical communication that is necessary to obtain non-classical fidelity in teleportation. We quantify the amount of classical communication that is sufficient for achieving non-classical fidelity for two independent 1-bit and single 2-bits noisy classical channels. It is shown that on average 0.208 bits of classical communication is sufficient to get non-classical fidelity. We also find the necessary amount of classical communication in case of isotropic transformation. Finally we study how the amount of sufficient classical communication increases with weakening of entanglement used in the teleportation process.Comment: Accepted in Quantum Info. Proces

PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer

Inhibition of WEE1 kinase by AZD1775 has shown promising results in clinical cancer trials, but markers predicting AZD1775 response are lacking. Here we analysed AZD1775 response in a panel of human breast cancer (BC) cell lines by global proteome/transcriptome profiling and identified two groups of basal-like BC (BLBCs): ‘PTEN low’ BLBCs were highly sensitive to AZD1775 and failed to recover following removal of AZD1775, while ‘PTEN high’ BLBCs recovered. AZD1775 induced phosphorylation of DNA-PK, protecting cells from replication-associated DNA damage and promoting cellular recovery. Deletion of DNA-PK or PTEN, or inhibition of DNA-PK sensitized recovering BLBCs to AZD1775 by abrogating replication arrest, allowing replication despite DNA damage. This was linked to reduced CHK1 activation, increased cyclin E levels and apoptosis. In conclusion, we identified PTEN and DNA-PK as essential regulators of replication checkpoint arrest in response to AZD1775 and defined PTEN as a promising biomarker for efficient WEE1 cancer therapy

Three-component particle image velocimetry in a generic can-type gas turbine combustor

A stereoscopic particle image velocimetry system was used to obtain the velocity field of a can-type combustor in the non-reacting condition. In order for these measurements to be taken, an optically accessible can-type forward flow combustor was manufactured. The combustor has a 10-vane swirler in the dome as well as a primary zone with six 9.5 mm holes, a secondary zone with eight 5 mm holes and a dilution zone with ten 11.8 mm holes. The two cooling rings have 30 and 50 × 1.2 mm holes and are placed between the three zones. The main flow features were captured such as the recirculation zones and jets. The more subtle features such as flow entering the swirler, entering the dilution holes from the annulus, and converging into smaller annuli around the secondary and dilution zones were also evident in some sections of the data. An unexpected flow recirculation was observed in the dilution zone. The departure of the flow from the recirculation regions to join the bulk flow in the dilution zone was also shown.The Defence Research and Development Board under Armscor order KT471006.http://www.uk.sagepub.com/journals/Journal202015hb2013ai201