Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors.

Abstract This study examines the regulation of cGMP by illumination and by calcium during signal transduction in vertebrate retinal photoreceptor cells. We employed an electropermeabilized rod outer segment (EP-ROS) preparation which permits perfusion of low molecular weight compounds into the cytosol while retaining many of the features of physiologically competent, intact rod outer segments (ROS). When nucleotide-depleted EP-ROS were incubated with MgGTP, time- and dose-dependent increases in intracellular cGMP levels were observed. The steady state cGMP concentration in EP-ROS (0.007 mol cGMP per mol rhodopsin) approached the cGMP concentration in intact ROS. Flash illumination of EP-ROS in a 250-nM free calcium medium resulted in a transient decrease in cGMP levels; this occurred in the absence of changes in calcium concentration. The kinetics of the cGMP response to flash illumination of EP-ROS were similar to that of intact ROS. To further examine the effects of calcium on cGMP metabolism, dark-adapted EP-ROS were incubated with MgGTP containing various concentrations of calcium. We observed a twofold increase in cGMP steady state levels as the free calcium was lowered from 1 μM to 20 nM; this increase was comparable to the behavior of intact ROS. Measurements of guanylate cyclase activity in EP-ROS showed a 3.5-fold increase in activity over this range of calcium concentrations, indicating a retention of calcium regulation of guanylate cyclase in EP-ROS preparations. Flash illumination of EP-ROS in either a 50- or 250-nM free calcium medium revealed a slowing of the recovery time course at the lower calcium concentration. This observation conflicts with any hypothesis whereby a reduction in free calcium concentration hastens the recovery of cytoplasmic cGMP levels, either by stimulating guanylate cyclase activity or by inhibiting phosphodiesterase activity. We conclude that changes in the intracellular calcium concentration during visual transduction may have more complex effects on the recovery of the photoresponse than can be accounted for solely by guanylate cyclase activation

Collective Excitations, NMR, and Phase Transitions in Skyrme Crystals

At Landau level filling factors near nu =1, quantum Hall ferromagnets form a Skyrme crystal state with quasi-long-range translational and non-collinear magnetic order. We develop an effective low energy theory which explains the presence in these systems of magnetic excitations at low energies below the Larmor gap (Delta) and which predicts a dramatic enhancement of the nuclear spin relaxation rate by a factor of 1000. The effective theory predicts a rich set of quantum and classical phase transitions. Based in part on accurate time-dependent Hartree-Fock calculations of the ordered state collective excitation spectrum, we discuss aspects of the T-nu-Delta crystal phase diagram.Comment: 4 pages REVTEX file and 3 postscript figure

Searching for Dust around Hyper Metal-Poor Stars

We examine the mid-infrared fluxes and spectral energy distributions for metal-poor stars with iron abundances [Fe/H] $\lesssim-5$, as well as two CEMP-no stars, to eliminate the possibility that their low metallicities are related to the depletion of elements onto dust grains in the formation of a debris disk. Six out of seven stars examined here show no mid-IR excess. These non-detections rule out many types of circumstellar disks, e.g. a warm debris disk ($T\!\le\!290$ K), or debris disks with inner radii $\le1$ AU, such as those associated with the chemically peculiar post-AGB spectroscopic binaries and RV Tau variables. However, we cannot rule out cooler debris disks, nor those with lower flux ratios to their host stars due to, e.g. a smaller disk mass, a larger inner disk radius, an absence of small grains, or even a multicomponent structure, as often found with the chemically peculiar Lambda Bootis stars. The only exception is HE0107-5240, for which a small mid-IR excess near 10 microns is detected at the 2-$\sigma$ level; if the excess is real and associated with this star, it may indicate the presence of (recent) dust-gas winnowing or a binary system.Comment: Accepted for publication in Ap

Satellite and ground radiotracking of elk

Radiotracking and monitoring of free-living animals in natural environments is providing an effective new technique for acquiring information on biological processes, including animal orientation and navigation. To test the practicability of extending the technique by using satellite systems for tracking animals, a female elk was instrumented with an electronic collar. It contained both the Interrogation Recording Location System (IRLS) transponder and a Craighead-Varney ground-tracking transmitter. The elk was successfully tracked and monitored by satellite during month of April 1970. This was the first time an animal had been tracked by satellite on the surface of the earth

The Ontology Lookup Service: more data and better tools for controlled vocabulary queries

The Ontology Lookup Service (OLS) (http://www.ebi.ac.uk/ols) provides interactive and programmatic interfaces to query, browse and navigate an ever increasing number of biomedical ontologies and controlled vocabularies. The volume of data available for querying has more than quadrupled since it went into production and OLS functionality has been integrated into several high-usage databases and data entry tools. Improvements have been made to both OLS query interfaces, based on user feedback and requirements, to improve usability and service interoperability and provide novel ways to perform queries

A Possible Massive Asteroid Belt Around zeta Lep

We have used the Keck I telescope to image at 11.7 microns and 17.9 microns the dust emission around zeta Lep, a main sequence A-type star at 21.5 pc from the Sun with an infrared excess. The excess is at most marginally resolved at 17.9 microns. The dust distance from the star is probably less than or equal to 6 AU, although some dust may extend to 9 AU. The mass of observed dust is \~10^22 g. Since the lifetime of dust particles is about 10,000 years because of the Poytning-Robertson effect, we robustly estimate at least 4 10^26 g must reside in parent bodies which may be asteroids if the system is in a steady state and has an age of ~300 Myr. This mass is approximately 200 times that contained within the main asteroid belt in our solar system.Comment: 12 pages, 3 figures, ApJL in pres