Vergleichende Untersuchungen von Pathogenitätsfaktoren in Escherichia coli aus unterschiedlichen Umwelthabitaten

Die Arbeit berichtet über den Nachweis potentieller Virulenzfaktoren der als ECOR (E. coli Reference Collection) und DEC (Diarrheagenic E. coli Collection) bezeichneten internationalen Referenzsammlungen, mittels molekularbiologischer Methoden. Das alpha-hly-Gen wurde in 10 der 72 ECOR- und in 9 der 78 DEC-Stämme identifiziert. Das EHEC-hlyA-Gen konnte in keinem der ECOR- aber in 11 der DEC-Stämme detektiert werden. Alle Stämme mit enterohämolytischer bzw. alpha-hämolytischer Phänotypausprägung besitzen auch das entsprechende Strukturgen. Das stx-Gen wurde in 1 ECOR- und in 17 DEC-Isolaten, das eae-Gen, in 3 ECOR- und in 55 DEC-Stämmen identifiziert. Das Markergen ureC tauchte in keinem der ECOR-, jedoch in 17 DEC-Stämmen auf. Die Ergebnisse zeigen, dass potentielle Virulenzgene darmpathogener E. coli, wie eae und stx, in den bisher als apathogen angesehenen ECOR-Stämmen vorkommen. Die als positiv befundenen ECOR-Stämme stellen somit ein generelles Risikopotential dar

Late 20th Century Indian Ocean Heat Content Gain Masked by Wind Forcing

Rapid increases in upper 700‐m Indian Ocean heat content (IOHC) since the 2000s have focused attention on its role during the recent global surface warming hiatus. Here, we use ocean model simulations to assess distinct multidecadal IOHC variations since the 1960s and explore the relative contributions from wind stress and buoyancy forcing regionally and with depth. Multidecadal wind forcing counteracted IOHC increases due to buoyancy forcing from the 1960s to the 1990s. Wind and buoyancy forcing contribute positively since the mid‐2000s, accounting for the drastic IOHC change. Distinct timing and structure of upper ocean temperature changes in the eastern and western Indian Ocean are linked to the pathway how multidecadal wind forcing associated with the Interdecadal Pacific Oscillation is transmitted and affects IOHC through local and remote winds. Progressive shoaling of the equatorial thermocline—of importance for low‐frequency variations in Indian Ocean Dipole occurrence—appears to be dominated by multidecadal variations in wind forcing

Controls on erosion patterns and sediment transport in a monsoonal, tectonically quiescent drainage, Song Gianh, central Vietnam

The Song Gianh is a small-sized (~3500 km2), monsoon-dominated river in northern central Vietnam that can be used to understand how topography and climate control continental erosion. We present major element concentrations, together with Sr and Nd isotopic compositions, of siliciclastic bulk sediments to define sediment provenance and chemical weathering intensity. These data indicate preferential sediment generation in the steep, wetter upper reaches of the Song Gianh. In contrast, detrital zircon U-Pb ages argue for significant flux from the drier, northern Rao Tro tributary. We propose that this mismatch represents disequilibrium in basin erosion patterns driven by changing monsoon strength and the onset of agriculture across the region. Detrital apatite fission track and 10Be data from modern sediment support slowing of regional bedrock exhumation rates through the Cenozoic. If the Song Gianh is representative of coastal Vietnam then the coastal mountains may have produced around 132 000–158 000 km3 of the sediment now preserved in the Song Hong-Yinggehai Basin (17–21 of the total), the primary depocenter of the Red River. This flux does not negate the need for drainage capture in the Red River to explain the large Cenozoic sediment volumes in that basin but does partly account for the discrepancy between preserved and eroded sediment volumes. OSL ages from terraces cluster in the Early Holocene (7.4–8.5 ka), Pre-Industrial (550–320 year BP) and in the recent past (ca. 150 year BP). The older terraces reflect high sediment production driven by a strong monsoon, whereas the younger are the product of anthropogenic impact on the landscape caused by farming. Modern river sediment is consistently more weathered than terrace sediment consistent with reworking of old weathered soils by agricultural disruption

Global ocean modeling and state estimation in support of climate research

During the last decade it has become obvious that the ocean circulation shows vigorous variability on a wide range of time and space scales and that the concept of a "sluggish" and slowly varying circulation is rather elusive. Increasing emphasis has to be put, therefore, on observing the rapidly changing ocean state on time scales ranging from weeks to decades and beyond, and on understanding the ocean's response to changing atmospheric forcing conditions. As outlined in various strategy and implementation documents (e.g., the implementation plans of WOCE, AMS, CLIVAR, and GODAE) a combination of the global ocean data sets with a state-of-the-art numerical circulation model is required to interpret the various diverse data sets and to produce the best possible estimates of the time-varying ocean circulation. The mechanism of ocean state estimates is a powerful tool for such a "synthesis" of observations, obtained on very complex space-time pattern, into one dynamically consistent picture of the global time-evolving ocean circulation. This process has much in common with ongoing analysis and reanalysis activities in the atmospheric community. But because the ocean is, and will remain for the foreseeable future, substantially under-sampled, the burden put on the modeling and estimations components is substantially larger than in the atmosphere. Moreover, the smaller dynamical eddy scales which need to be properly parameterized or resolved in ocean model simulations, put stringent requirements on computational resources for ongoing and participated climate research

ENSO-driven interhemispheric Pacific mass transports

Previous studies have shown that ENSO's anomalous equatorial winds, including the observed southward shift of zonal winds that occurs around the event peak, can be reconstructed with the first two Empirical Orthogonal Functions (EOFs) of equatorial region wind stresses. Using a high-resolution ocean general circulation model, we investigate the effect of these two EOFs on changes in warm water volume (WWV), interhemispheric mass transports, and Indonesian Throughflow (ITF). Wind stress anomalies associated with the first EOF produce changes in WWV that are dynamically consistent with the conceptual recharge oscillator paradigm. The ITF is found to heavily damp these WWV changes, reducing their variance by half. Wind stress anomalies associated with the second EOF, which depicts the southward wind shift, are responsible for WWV changes that are of comparable magnitude to those driven by the first mode. The southward wind shift is also responsible for the majority of the observed interhemispheric upper ocean mass exchanges. These winds transfer mass between the Northern and the Southern Hemisphere during El Niño events. Whilst water is transferred in the opposite direction during La Niña events, the magnitude of this exchange is roughly half of that seen during El Niño events. Thus, the discharging of WWV during El Niño events is meridionally asymmetric, while the WWV recharging during a La Niña event is largely symmetric. The inclusion of the southward wind shift is also shown to allow ENSO to exchange mass with much higher latitudes than that allowed by the first EOF alone

Pacific-to-Indian Ocean connectivity: Tasman leakage, Indonesian Throughflow, and the role of ENSO

The upper ocean circulation of the Pacific and Indian Oceans is connected through both the Indonesian Throughflow north of Australia and the Tasman leakage around its south. The relative importance of these two pathways is examined using virtual Lagrangian particles in a high-resolution nested ocean model. The unprecedented combination of a long integration time within an eddy-permitting ocean model simulation allows the first assessment of the interannual variability of these pathways in a realistic setting. The mean Indonesian Throughflow, as diagnosed by the particles, is 14.3 Sv, considerably higher than the diagnosed average Tasman leakage of 4.2 Sv. The time series of Indonesian Throughflow agrees well with the Eulerian transport through the major Indonesian Passages, validating the Lagrangian approach using transport-tagged particles. While the Indonesian Throughflow is mainly associated with upper ocean pathways, the Tasman leakage is concentrated in the 400–900 m depth range at subtropical latitudes. Over the effective period considered (1968–1994), no apparent relationship is found between the Tasman leakage and Indonesian Throughflow. However, the Indonesian Throughflow transport correlates with ENSO. During strong La Niñas, more water of Southern Hemisphere origin flows through Makassar, Moluccas, Ombai, and Timor Straits, but less through Moluccas Strait. In general, each strait responds differently to ENSO, highlighting the complex nature of the ENSO-ITF interaction

Changes of anthropogenic CO₂ and CFCs in the North Atlantic between 1981 and 2004

We compare total dissolved inorganic carbon (DIC) and chlorofluorocarbon (CFC) measurements in the northwest Atlantic made during the Transient Tracers in the Ocean, North Atlantic Study (TTO‐NAS) in 1981 with modern measurements from a cruise in 2004. The observed changes in the DIC and CFC fields are compared to those predicted from an eddy‐permitting ocean circulation model. The rapid, but time‐variable, atmospheric CFC increase in relation to the relatively steady anthropogenic CO2 increase influences the relationship between the observed uptake of DIC and CFC. We demonstrate the importance of ocean mixing in the calculation of anthropogenic CO2 (Cant) based on transient tracer data by comparing our observations to a “no‐mixing” scenario. We further find that the Cant is in transient steady state in the North Atlantic; that is, the Cant concentration increases proportionally over time through the whole water column in a manner that is directly related to the time‐dependent surface concentration

RNase1 as a potential mediator of remote ischaemic preconditioning for cardioprotection

© The Author 2015. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. OBJECTIVES: Remote ischaemic preconditioning (RIPC) is a non-invasive and virtually cost-free strategy for protecting the heart against acute ischaemia-reperfusion injury (IRI). We have recently shown that the inhibition of extracellular RNA (eRNA) using non-toxic RNase1 protected the heart against acute IRI, reduced myocardial infarct (MI) size and preserved left ventricular systolic function in rodent animal MI models. Based on this previous work in animals, the role of the eRNA/RNase1 system in cardiac RIPC in humans should be defined. METHODS: Fourteen patients underwent cardiac surgery without RIPC; from each patient, six separate 5 ml blood specimens from radial artery and two blood specimens from coronary sinus at different time points during heart surgery were taken. Six healthy donors received RIPC (4 × 5 min upper limb ischaemia); blood parameters were quantified before and after RIPC. Twelve patients underwent cardiac surgery of which 6 received RIPC, whereas the remaining 6 were exposed to sham procedure. Circulating eRNA was quantified in plasma from arterial and coronary sinus blood obtained from patients undergoing cardiac by standard procedures. Tumour necrosis factor-α (TNF-α) production by heart tissue was assessed by enzyme-linked immuno-sorbent assay; RNase activity was quantified by an enzymatic assay. RESULTS: Before surgery, eRNA levels were similar in both groups (14 ± 6 vs 13 ± 5 ng/ml; P = 0.9967). In patients without RIPC, arterial eRNA levels rose during surgery (87 ± 12 ng/ml) and peaked after (127 ± 11 ng/ml) aortic declamping; accordingly, eRNA levels in coronary sinus blood were significantly higher (206 ± 32 ng/ml; P = 0.0129) than that in radial artery. Moreover, significant elevation of TNF-α (36 ± 6 ng/ml; P = 0.0059) particularly in coronary sinus blood after opening of the aortic clamping was observed. Interestingly, applying a RIPC protocol significantly increased levels of plasma endogenous vascular RNase1 by >7-fold, and the levels of arterial (31 ± 7 ng/ml; P = 0.0024) and coronary sinus (37 ± 9 ng/ml; P < 0.0001) circulating eRNA, as well as circulating TNF-α (20 ± 4 ng/ml; P = 0.0050) levels were significantly reduced. CONCLUSIONS: Upon RIPC, the level of cardioprotective RNase1 increased, while the concentration of damaging eRNA and TNF-α decreased. The present findings imply a significant contribution of the RIPC-dependent (endothelial) RNase1 for improving the outcome of cardiac surgery. However, the exact mechanism of RNase1-induced cardioprotection still remains to be explored

Spatial and temporal patterns of pore water chemistry in the inter-tidal zone of a high energy beach

Submarine groundwater discharge (SGD) is a ubiquitous source of meteoric fresh groundwater and recirculating seawater to the coastal ocean. Due to the hidden distribution of SGD, as well as the hydraulic- and stratigraphy-driven spatial and temporal heterogeneities, one of the biggest challenges to date is the correct assessment of SGD-driven constituent fluxes. Here, we present results from a 3-dimensional seasonal sampling campaign of a shallow subterranean estuary in a high-energy, meso-tidal beach, Spiekeroog Island, Northern Germany. We determined beach topography and analyzed physico-chemical and biogeochemical parameters such as salinity, temperature, dissolved oxygen, Fe(II) and dissolved organic matter fluorescence (FDOM). Overall, the highest gradients in pore water chemistry were found in the cross-shore direction. In particular, a strong physico-chemical differentiation between the tidal high water and low water line was found and reflected relatively stable in- and exfiltrating conditions in these areas. Contrastingly, in between, the pore water compositions in the existing foreshore ridge and runnel system were very heterogeneous on a spatial and temporal scale. The reasons for this observation may be the strong morphological changes that occur throughout the entire year, which affect the exact locations and heights of the ridge and runnel structures and associated flow paths. Further, seasonal changes in temperature and inland hydraulic head, and the associated effect on microbial mediated redox reactions likely overprint these patterns. In the long-shore direction the pore water chemistry varied less than the along the cross-shore direction. Variation in long-shore direction was probably occurring due to topography changes of the ridge-runnel structure and a physical heterogeneity of the sediment, which produced non-uniform groundwater flow conditions. We conclude that on meso-tidal high energy beaches, the rapidly changing beach morphology produces zones with different approximations to steady-state conditions. Therefore, we suggest that zone-specific endmember sampling is the optimal strategy to reduce uncertainties of SGD-driven constituent fluxes

Shell structure of superheavy nuclei in self-consistent mean-field models

We study the extrapolation of nuclear shell structure to the region of superheavy nuclei in self-consistent mean-field models -- the Skyrme-Hartree-Fock approach and the relativistic mean-field model -- using a large number of parameterizations. Results obtained with the Folded-Yukawa potential are shown for comparison. We focus on differences in the isospin dependence of the spin-orbit interaction and the effective mass between the models and their influence on single-particle spectra. While all relativistic models give a reasonable description of spin-orbit splittings, all non-relativistic models show a wrong trend with mass number. The spin-orbit splitting of heavy nuclei might be overestimated by 40%-80%. Spherical doubly-magic superheavy nuclei are found at (Z=114,N=184), (Z=120,N=172) or (Z=126,N=184) depending on the parameterization. The Z=114 proton shell closure, which is related to a large spin-orbit splitting of proton 2f states, is predicted only by forces which by far overestimate the proton spin-orbit splitting in Pb208. The Z=120 and N=172 shell closures predicted by the relativistic models and some Skyrme interactions are found to be related to a central depression of the nuclear density distribution. This effect cannot appear in macroscopic-microscopic models which have a limited freedom for the density distribution only. In summary, our findings give a strong argument for (Z=120,N=172) to be the next spherical doubly-magic superheavy nucleus.Comment: 22 pages REVTeX, 16 eps figures, accepted for publication in Phys. Rev.