Widespread recombination, reassortment, and transmission of unbalanced compound viral genotypes in natural arenavirus infections.

Arenaviruses are one of the largest families of human hemorrhagic fever viruses and are known to infect both mammals and snakes. Arenaviruses package a large (L) and small (S) genome segment in their virions. For segmented RNA viruses like these, novel genotypes can be generated through mutation, recombination, and reassortment. Although it is believed that an ancient recombination event led to the emergence of a new lineage of mammalian arenaviruses, neither recombination nor reassortment has been definitively documented in natural arenavirus infections. Here, we used metagenomic sequencing to survey the viral diversity present in captive arenavirus-infected snakes. From 48 infected animals, we determined the complete or near complete sequence of 210 genome segments that grouped into 23 L and 11 S genotypes. The majority of snakes were multiply infected, with up to 4 distinct S and 11 distinct L segment genotypes in individual animals. This S/L imbalance was typical: in all cases intrahost L segment genotypes outnumbered S genotypes, and a particular S segment genotype dominated in individual animals and at a population level. We corroborated sequencing results by qRT-PCR and virus isolation, and isolates replicated as ensembles in culture. Numerous instances of recombination and reassortment were detected, including recombinant segments with unusual organizations featuring 2 intergenic regions and superfluous content, which were capable of stable replication and transmission despite their atypical structures. Overall, this represents intrahost diversity of an extent and form that goes well beyond what has been observed for arenaviruses or for viruses in general. This diversity can be plausibly attributed to the captive intermingling of sub-clinically infected wild-caught snakes. Thus, beyond providing a unique opportunity to study arenavirus evolution and adaptation, these findings allow the investigation of unintended anthropogenic impacts on viral ecology, diversity, and disease potential

A photometric and spectroscopic study of dwarf and giant galaxies in the Coma cluster - V. Dependence of the spectroscopic properties on location in the cluster

We investigate the radial dependence of the spectroscopic properties, in particular the Mg2, and H beta spectroscopic indices, in a sample of galaxies spanning a wide range of absolute luminosity in the Coma cluster. After allowing for the magnitude dependence of these indices, we find a significant gradient in Mg2, in the sense that galaxies in the core of the cluster have stronger Mg2. We find only weak gradients in and H beta. Using the model grids presented in an earlier paper in this series, we attribute the Mg2 gradient to changes in metal abundance. One possible mechanism to create this abundance gradient is pressure confinement by the intracluster medium of material from Supernova driven winds early in the history of the galaxies.Comment: 12 pages, 11 figures, accepted by Ap. J. (main journal

Keck Spectroscopy of Redshift z~3 Galaxies in the Hubble Deep Field

We have obtained spectra with the 10-m Keck telescope of a sample of 24 galaxies having colors consistent with star-forming galaxies at redshifts 2<z<4.5 in the Hubble Deep Field (HDF). Eleven of these galaxies are confirmed to be at high redshift (median z=3.0), one is at z=0.5, and the other 12 have uncertain redshifts but have spectra consistent with their being at z>2. Combined with 5 previously confirmed high-redshift galaxies in the HDF, the 16 confirmed sources yield a comoving volume density of n>2.5^-4 h50^3 Mpc^-3 for q0=0.05, or n>1.2^-3 h50^3 Mpc^-3 for q0=0.5. These densities are comparable to estimates of the local volume density of galaxies brighter than L*, and could be almost three times higher still if all 29 of the unconfirmed candidates in our original sample are indeed also at high redshift. The galaxies are small but luminous, with half-light radii 1.8 < r(1/2) < 6.5 h50^-1 kpc and absolute magnitudes -21.5 > M_B > -23. The HST images show a wide range of morphologies, including several with very close, small knots of emission embedded in wispy extended structures. Using rest-frame UV continuum fluxes with no dust correction, we calculate star formation rates in the range 7 - 24 or 3 - 9 h50^-2 Msun/yr for q0=0.05 and q0=0.5, respectively. The variety of morphologies and the high number density of z=3 galaxies in the HDF suggest that they represent a range of physical processes and stages of galaxy formation and evolution, rather than any one class of object, such as massive ellipticals. A key issue remains the measurement of masses. These high-redshift objects are likely to be the low-mass, starbursting building blocks of more massive galaxies seen today.Comment: 45 pages, AASLaTeX, 3 postscript tables, 2 JPG + 3 GIF + 5 encapsulated postscript figures. To appear in The Astrophysical Journal. Also available at http://www.ucolick.org/~deep/papers/papers.html and http://www.ucolick.org/~james/papers/hdf/hdf.htm

The Color-Magnitude Relation in CL 1358+62 at z=0.33: Evidence for Significant Evolution in the S0 Population

We use a large mosaic of HST WFPC2 images to measure the colors and morphologies of 194 spectroscopically confirmed members of the rich galaxy cluster CL1358+62 at z=0.33. We study the color-magnitude (CM) relation as a function of radius in the cluster. The intrinsic scatter in the restframe B-V CM relation of the elliptical galaxies is very small: ~0.022 magnitudes. The CM relation of the ellipticals does not depend significantly on the distance from the cluster center. In contrast, the CM relation for the S0 galaxies does depend on radius: the S0s in the core follow a CM relation similar to the ellipticals, but at large radii (R>0.7Mpc) the S0s are systematically bluer and the scatter in the CM relation approximately doubles to ~0.043 magnitudes. The blueing of the S0s is significant at the 95% confidence level. These results imply that the S0 galaxies in the outer parts of the cluster have formed stars more recently than the S0s in the inner parts. A likely explanation is that clusters at z=0.33 continue to accrete galaxies and groups from the field and that infall extinguishes star formation. The apparent homogeneity of the elliptical galaxy population implies that star formation in recently accreted ellipticals was terminated well before accretion occurred. We have constructed models to explore the constraints that these observations place on the star formation history of cluster galaxies. We conclude that the population of S0s in clusters is likely to evolve as star forming galaxies are converted into passively evolving galaxies. Assuming a constant accretion rate after z=0.33, we estimate ~15% of the present day early-type galaxy population in rich clusters was accreted between z=0.33 and z=0. The ellipticals (and the brightest S0s) are probably a more stable population, at least since z=0.6.Comment: Accepted for publication in the ApJ. 20 pages, 12 figures. Full version and plates available at http://www.astro.rug.nl/~dokkum/papers.htm

Transportation Energy Pathways LDRD.

This report presents a system dynamics based model of the supply-demand interactions between the USlight-duty vehicle (LDV) fleet, its fuels, and the corresponding primary energy sources through the year2050. An important capability of our model is the ability to conduct parametric analyses. Others have reliedupon scenario-based analysis, where one discrete set of values is assigned to the input variables and used togenerate one possible realization of the future. While these scenarios can be illustrative of dominant trendsand tradeoffs under certain circumstances, changes in input values or assumptions can have a significantimpact on results, especially when output metrics are associated with projections far into the future. Thistype of uncertainty can be addressed by using a parametric study to examine a range of values for the inputvariables, offering a richer source of data to an analyst.The parametric analysis featured here focuses on a trade space exploration, with emphasis on factors thatinfluence the adoption rates of electric vehicles (EVs), the reduction of GHG emissions, and the reduction ofpetroleum consumption within the US LDV fleet. The underlying model emphasizes competition between13 different types of powertrains, including conventional internal combustion engine (ICE) vehicles, flex-fuel vehicles (FFVs), conventional hybrids(HEVs), plug-in hybrids (PHEVs), and battery electric vehicles(BEVs).We find that many factors contribute to the adoption rates of EVs. These include the pace of technologicaldevelopment for the electric powertrain, battery performance, as well as the efficiency improvements inconventional vehicles. Policy initiatives can also have a dramatic impact on the degree of EV adoption. Theconsumer effective payback period, in particular, can significantly increase the market penetration rates ifextended towards the vehicle lifetime.Widespread EV adoption can have noticeable impact on petroleum consumption and greenhouse gas(GHG) emission by the LDV fleet. However, EVs alone cannot drive compliance with the most aggressiveGHG emission reduction targets, even as the current electricity source mix shifts away from coal and towardsnatural gas. Since ICEs will comprise the majority of the LDV fleet for up to forty years, conventional vehicleefficiency improvements have the greatest potential for reductions in LDV GHG emissions over this time.These findings seem robust even if global oil prices rise to two to three times current projections. Thus,investment in improving the internal combustion engine might be the cheapest, lowest risk avenue towardsmeeting ambitious GHG emission and petroleum consumption reduction targets out to 2050.3 AcknowledgmentThe authors would like to thank Dr. Andrew Lutz, Dr. Benjamin Wu, Prof. Joan Ogden and Dr. ChristopherYang for their suggestions over the course of this project. This work was funded by the Laboratory DirectedResearch and Development program at Sandia National Laboratories.

The Sloan Lens ACS Survey. VII. Elliptical Galaxy Scaling Laws from Direct Observational Mass Measurements

We use a sample of 53 massive early-type strong gravitational lens galaxies with well-measured redshifts (ranging from z=0.06 to 0.36) and stellar velocity dispersions (between 175 and 400 km/s) from the Sloan Lens ACS (SLACS) Survey to derive numerous empirical scaling relations. The ratio between central stellar velocity dispersion and isothermal lens-model velocity dispersion is nearly unity within errors. The SLACS lenses define a fundamental plane (FP) that is consistent with the FP of the general population of early-type galaxies. We measure the relationship between strong-lensing mass M_lens within one-half effective radius (R_e/2) and the dimensional mass variable M_dim = G^-1 sigma_e2^2 R_e/2 to be log_10 [M_lens/10^11 M_Sun] = (1.03 +/- 0.04) log_10 [M_dim/10^11 M_Sun] + (0.54 +/- 0.02) (where sigma_e2 is the projected stellar velocity dispersion within R_e/2). The near-unity slope indicates that the mass-dynamical structure of massive elliptical galaxies is independent of mass, and that the "tilt" of the SLACS FP is due entirely to variation in total (luminous plus dark) mass-to-light ratio with mass. Our results imply that dynamical masses serve as a good proxies for true masses in massive elliptical galaxies. Regarding the SLACS lenses as a homologous population, we find that the average enclosed 2D mass profile goes as log_10 [M(<R)/M_dim] = (1.10 +/- 0.09) log_10 [R/R_e] + (0.85 +/- 0.03), consistent with an isothermal (flat rotation curve) model when de-projected into 3D. This measurement is inconsistent with the slope of the average projected aperture luminosity profile at a confidence level greater than 99.9%, implying a minimum dark-matter fraction of f_DM = 0.38 +/- 0.07 within one effective radius. (abridged)Comment: 13 pages emulateapj; accepted for publication in the Ap

Redshift z ~ 1 Field Galaxies Observed with the Keck Telescope and the HST

We report results based on 35 new spectroscopic redshifts obtained with the Keck Telescope for field galaxies that also have photometry and morphology from survey images taken by the refurbished HST. A sample of 24 redshifts for galaxies fainter than I = 22 has a median redshift of z ~ 0.81. This result is inconsistent with the lower median redshift of z ~ 0.6 predicted by the ``maximal merger models'' of Carlberg (1996), which otherwise fit existing data. The data match an extrapolation of the CFRS, as well as predictions of certain mild luminosity-evolution models. Nearly half of the redshifts lie in two structures at z ~ 0.81 and z ~ 1.0, showing the presence of high density concentrations spanning scales of ~ 1/h Mpc, i.e., the size of groups. We find emission lines or the presence of possible neighbors in 7 of 9 otherwise luminous galaxies with red central regions at redshifts beyond z ~ 0.7. We also note a diversity of morphological types among blue galaxies at z ~ 1, including small compact galaxies, ``chains,'' and ``blue nucleated galaxies.'' These morphologies are found among local, but generally less luminous, galaxies. Distant blue galaxies also include apparently normal late-type spirals. These findings could imply modest bursts of star formation caused by mergers or interactions of small, gas-rich galaxies with each other or with larger, well-formed galaxies. This first glimpse of very faint z ~ 1 field galaxies of diverse colors and morphologies suggests that a mixture of physical processes is at work in the formation and evolution of faint field galaxies.Comment: 20 pages (31 with table and GIF figures). Full text and postscript figures are available at http://www.ucolick.org/~nicole/pubs/pubs.html#gs1 and http://tarkus.pha.jhu.edu/deep/publications.html . Accepted by The Astrophysical Journa

Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A

International audienceAims/Hypothesis: Reduced skeletal muscle insulin sensitivity is a feature associated with sustained exposure to excess saturated fatty acids (SFA), whereas mono and polyunsaturated fatty acids (MUFA and PUFA) not only improve insulin sensitivity but blunt SFA-induced insulin resistance. The mechanisms by which MUFAs and PUFAs institute these favourable changes remain unclear, but may involve stimulating insulin signalling by counter-modulation/repression of protein phosphatase 2A (PP2A). This study investigated the effects of oleic acid (OA; a MUFA), linoleic acid (LOA; a PUFA) and palmitate (PA; a SFA) in cultured myotubes and determined whether changes in insulin signalling can be attributed to PP2A regulation. Principal Findings: We treated cultured skeletal myotubes with unsaturated and saturated fatty acids and evaluated insulin signalling, phosphorylation and methylation status of the catalytic subunit of PP2A. Unlike PA, sustained incubation of rat or human myotubes with OA or LOA significantly enhanced Akt-and ERK1/2-directed insulin signalling. This was not due to heightened upstream IRS1 or PI3K signalling nor to changes in expression of proteins involved in proximal insulin signalling, but was associated with reduced dephosphorylation/inactivation of Akt and ERK1/2. Consistent with this, PA reduced PP2Ac demethylation and tyrosine 307 phosphorylation-events associated with PP2A activation. In contrast, OA and LOA strongly opposed these PA-induced changes in PP2Ac thus exerting a repressive effect on PP2A.Conclusions/Interpretation: Beneficial gains in insulin sensitivity and the ability of unsaturated fatty acids to oppose palmitate-induced insulin resistance in muscle cells may partly be accounted for by counter-modulation of PP2A

The Role of Genomics in the Identification, Prediction, and Prevention of Biological Threats

In all likelihood, it is only a matter of time before our public health system will face a major biological threat, whether intentionally dispersed or originating from a known or newly emerging infectious disease. It is necessary not only to increase our reactive “biodefense,” but also to be proactive and increase our preparedness. To achieve this goal, it is essential that the scientific and public health communities fully embrace the genomic revolution, and that novel bioinformatic and computing tools necessary to make great strides in our understanding of these novel and emerging threats be developed. Genomics has graduated from a specialized field of science to a research tool that soon will be routine in research laboratories and clinical settings. Because the technology is becoming more affordable, genomics can and should be used proactively to build our preparedness and responsiveness to biological threats. All pieces, including major continued funding, advances in next-generation sequencing technologies, bioinformatics infrastructures, and open access to data and metadata, are being set in place for genomics to play a central role in our public health system