Applications of piezoresponse force microscopy in materials research: from inorganic ferroelectrics to biopiezoelectrics and beyond

Piezoresponse force microscopy (PFM) probes the mechanical deformation of a sample in response to an electric field applied with the tip of an atomic force microscope. Originally developed more than two decades ago to study ferroelectric materials, this technique has since been used to probe electromechanical functionality in a wide range of piezoelectric materials including organic and biological systems. Piezoresponse force microscopy has also been demonstrated as a useful tool to detect mechanical strain originating from electrical phenomena in non-piezoelectric materials. Parallelling advances in analytical and numerical modelling, many technical improvements have been made in the last decade: switching spectroscopy PFM allows the polarisation switching properties of ferroelectrics to be resolved in real space with nanometric resolution, while dual ac resonance tracking and band excitation PFM have been used to improve the signal-to-noise ratio. In turn, these advances have led to increasingly large multidimensional data sets containing more complete information on the properties of the sample studied. In this review, PFM operation and calibration are described, and recent advances in the characterisation of electromechanical coupling using PFM are presented. The breadth of the systems covered highlights the versatility and wide applicability of PFM in fields as diverse as materials engineering and nanomedicine. In each of these fields, combining PFM with complementary techniques is key to develop future insight into the intrinsic properties of the materials as well as for device applications.Science Foundation IrelandSwiss National Science Foundatio

Non-destructive determination of collagen fibril width in extruded collagen fibres by piezoresponse force microscopy

Extruded collagen fibres are a promising platform for tissue engineering applications. Ensuring that the functional properties of the engineered tissues possess similar structural properties as native tissues is important for biomedical applications. Advanced imaging tools including scanning electron microscopy (SEM) and atomic force microscopy (AFM) have revealed the structural features of collagen fibrils within such fibres; however, these techniques often require modification steps that can alter the sample in the process. Here, lateral piezoresponse force microscopy (LPFM), which is sensitive to the polar orientation of piezoelectric collagen fibrils, is demonstrated as a promising tool to assess the width of individual fibrils and moreover map their organisation and polar orientation without altering the sample. Within the fibres studied, the collagen fibrils showed a highly anisotropic arrangement with preferred alignment along the length of the fibre. Fibril widths of 74 ± 18 nm and 73 ± 19 nm in untreated and bleached fibres, respectively, were measured from LPFM amplitude images. These values agreed with values from SEM (70 ± 10 nm) and AFM (71 ± 19 nm) measurements that could only be obtained from bleached fibres.Department of Agriculture, Food and the MarineEuropean Commission Horizon 2020Science Foundation IrelandTeagascMinistry of Higher Education of Saudi Arabi

Nanoscale Piezoelectric Properties of Self-Assembled Fmoc-FF Peptide Fibrous Networks

Fibrous peptide networks, such as the structural framework of self-assembled fluorenylmethyloxycarbonyl diphenylalanine (Fmoc-FF) nanofibrils, have mechanical properties that could successfully mimic natural tissues, making them promising materials for tissue engineering scaffolds. These nanomaterials have been determined to exhibit shear piezoelectricity using piezoresponse force microscopy, as previously reported for FF nanotubes. Structural analyses of Fmoc-FF nanofibrils suggest that the observed piezoelectric response may result from the noncentrosymmetric nature of an underlying β-sheet topology. The observed piezoelectricity of Fmoc-FF fibrous networks is advantageous for a range of biomedical applications where electrical or mechanical stimuli are required.European Commission - European Regional Development FundEuropean Commission - Seventh Framework Programme (FP7)Science Foundation IrelandProgramme for Research in Third Level Institution

Piezoresponse Force Microscopy for Bioelectromechanics

Electromechanical coupling, including piezoelectricity, ferroelectricity, and flexoelectricity, is present in a wide range of organic materials. Such phenomena have been postulated to have a functional role in biological systems, where e.g. conformational changes in proteins can be electrically activated. Investigating the electromechanical properties of biological materials at the micro to nanoscale is therefore crucial for understanding the possible biofunctionality of piezoelectricity and for exploiting such properties in, e.g. sensing, actuating or energy harvesting applications. This chapter provides an overview of the use of piezoresponse force microscopy in the investigation of biomaterials to further our understanding of bioelectromechanics

Nanoscale Piezoelectric Properties of Self-Assembled Fmoc-FF Peptide Fibrous Networks

Fibrous peptide networks, such as the structural framework of self-assembled fluorenylmethyloxycarbonyl diphenylalanine (Fmoc-FF) nanofibrils, have mechanical properties that could successfully mimic natural tissues, making them promising materials for tissue engineering scaffolds. These nanomaterials have been determined to exhibit shear piezoelectricity using piezoresponse force microscopy, as previously reported for FF nanotubes. Structural analyses of Fmoc-FF nanofibrils suggest that the observed piezoelectric response may result from the noncentrosymmetric nature of an underlying beta-sheet topology. The observed piezoelectricity of Fmoc-FF fibrous networks is advantageous for a range of biomedical applications where electrical or mechanical stimuli are required