ENDOMETRIOSE: REPERCUSSÕES NA VIDA PROFISSIONAL, SOCIAL E SEXUAL DAS MULHERES.

RESUMO A endometriose é uma condição inflamatória crônica caracterizada pela presença de tecido endometrial fora do útero, afetando entre 1,5% à 15% das mulheres que freqüentam serviços terciários (JOHNSON et al, 2013). Diversos estudos evidenciam as consequências psicossociais da endometriose. Pesquisas em clínicas especializadas realizadas por Culley et al. (2013) sugerem que mulheres com endometriose apresentam diminuição da qualidade de vida, da saúde mental e do bem-estar emocional quando comparadas com a população geral. Palavras-chave: Endometriose; Bem-estar; Compreensão psicanalítica. Parte da dissertação: BONFIM, M.S. Vivências socioafetivas de pacientes com endometriose: Uma compreensão psicanalítica clássica. Universidade Federal do Amazonas, Faculdade de Psicologia, Programa de Mestrado em Psicologia, 2019

Spleen proteomics data from high fat diet fed mice

The composition of the diet affects many processes in the body, including body weight and endocrine system. We have previously shown that dietary fat also affects the immune system. Mice fed high fat diet rich in polyunsaturated fatty acids survive S. aureus infection to a much greater extent than mice fed high fat diet rich in saturated fatty acids. Here we present data regarding the dietary effects on protein expression in spleen from mice fed three different diets, I) low fat/chow diet (LFD, n = 4), II) high fat diet rich in saturated fatty acids (HFD-S, n = 4) and III) high fat diet rich in polyunsaturated fatty acids (HFD-P, n = 4). We performed mass spectrophotometry based quantitative proteomics analysis of isolated spleen by implementing the isobaric tags for relative and absolute quantification (iTRAQ) approach. Mass spectrometry data were analyzed using Proteome Discoverer 2.4 software using the search engine mascot against Mus musculus in SwissProt. 924 proteins are identified in all sets (n = 4) for different dietary effects taken for statistical analysis using Qlucore Omics Explorer software. Only 20 proteins were found to be differentially expressed with a cut-off value of false discovery rate < 0.1 (q-value) when comparing HFD-S and HFD-P but no differentially expressed proteins were found when LFD was compared with HFD-P or HFD-S. The identified proteins and statistical analysis comparing HFD-S and HFD-P diets are available as a supplementary file S1. We identified a subset of proteins that showed an inverse expression pattern between two high fat diets. These differentially expressed proteins were further classified by gene ontology for their role in biological processes and molecular functions. Mass spectrometry raw data are also available via ProteomeXchange with identifier PXD020365

¡Atención Poli, con la vista en el riesgo! cuentos para seguritos

El contar historias o el storytelling, aparece como una herramienta para autores que no solo buscan el conectar de forma más auténtica con sus lectores; sino que buscan ir más allá al comprender cómo el cerebro procesa esta poderosa forma de comunicación. (Suzuki, W., Feliú-Mójer, M., Hasson, U., Yehuda, R., & Zarate, J. 2018). Esta nueva forma de contar algo, es presentada aquí, en la primera compilación de cuentos como resultado de trabajos de módulos que tratan sobre el cuerpo humano, donde el estudio y la comprensión de la forma cómo funciona nuestro organismo, es uno de los principales objetivos. Además, surge como estrategia pedagógica, que precisamente busca incentivar el desarrollo de la creatividad de los estudiantes dentro de módulos que contienen un importante abordaje de temas y conceptos como base para su desarrollo, módulos que, al incluir un alto contenido teórico, se presentan como retos para que sus temas sean interiorizados por los estudiantes. De este modo, cuando se acude a el uso de la creatividad a través del storytelling, donde se expresan ideas relacionadas con el cuerpo humano, los cuentos realizados por estudiantes aquí presentes logran mostrar cómo emplean dichos conocimientos adquiridos, organizan ideas y crean cuentos que permiten al lector recrease, conociendo diferentes aspectos sobre nuestro cuerpo y a su vez dejándose llevar por la imaginación de los autores

¿Qué queda de mí?

Este libro es una reclamación a quienes hemos sido, somos o seremos docentes. A quienes no hemos respetado a las personas que se han puesto junto a nosotros y nosotras, confiando su bien más preciado: la libertad. Estas páginas denuncian cada vez que convertimos una visión en la visión, una emoción en la emoción, un saber en el saber, un comportamiento en el comportamiento. Es un grito contra la imposición, la normalización, la neutralización y la universalización de una perspectiva particular. Una pugna contra cada proceso que no se ha conectado con las vidas de los aprendices. Un texto colaborativo realizado por alumnado de Educación y Cambio Social en el Grado en Educación Infantil de la Universidad de Málaga y coordinado por Ignacio Calderón Almendros

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Salmonella typhimurium is a facultative intracellular bacterium that causes gastroenteritis in humans. After invasion of the lamina propria, S. typhimurium bacteria are quickly detected and phagocytized by macrophages, and contained in vesicles known as phagosomes in order to be degraded. Isolation of S. typhimurium-containing phagosomes have been widely used to study how S. typhimurium infection alters the process of phagosome maturation to prevent bacterial degradation. Classically, the isolation of bacteria-containing phagosomes has been performed by sucrose gradient centrifugation. However, this process is time-consuming, and requires specialized equipment and a certain degree of dexterity. Described here is a simple and quick method for the isolation of S. typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin-streptavidin-conjugated magnetic beads. Phagosomes obtained by this method can be suspended in any buffer of choice, allowing the utilization of isolated phagosomes for a broad range of assays, such as protein, metabolite, and lipid analysis. In summary, this method for the isolation of S. typhimurium-containing phagosomes is specific, efficient, rapid, requires minimum equipment, and is more versatile than the classical method of isolation by sucrose gradient-ultracentrifugation

Impacto de la rehabilitación neuropsicológica en la calidad de vida en pacientes con daño cerebral adquirido: una revisión de la literatura

Esta investigación se centró en la búsqueda e identificación de los factores que influyen en calidad de vida de pacientes con daño cerebral adquirido (DCA) que se encuentren bajo algún tratamiento de rehabilitación neuropsicológica, con el objetivo de abordar su efectividad, analizar variables y clasificar perspectivas (modelos, propuestas) de intervención, descritos en estudios científicos. Se realizó un estudio de tipo revisión sistemática que implico la recopilación, extracción y organización de distintos datos en torno a una pregunta de investigación, mediante la búsqueda en base de datos; siguiendo de forma más específica las directrices de PRISMA. Se pre-seleccionaron 115 artículos de forma manual en la fase de búsqueda, que posteriormente pasaron por procesos de filtrado para arrojar un total de 30 artículos finales presentes en esta revisión. Se concluyó que las investigaciones de enfoque holístico, intervenciones multidisciplinarias e integrales, favorecen la calidad de vida en pacientes con DCA y la neuroplasticidad en la recuperación de funciones cognitivas previas; perdidas después de del DCA, de acuerdo a los distintos artículos científicos analizados. Además, se demostró que no existe suficiente investigación sobre esta temática en Latinoamérica, y que la mayoría de investigaciones provienen de Europa y NorteaméricaThis investigation was focused on the search and identification of the factors that influence the quality of life of patients with acquired brain injury (ABI). Patients who are undergoing some neuropsychological rehabilitation treatment, with the aim of addressing its effectiveness, analyzing variables and classify perspectives (models, proposals) of intervention, described in scientific studies. It was developed a systematic review tthat involved the search, collection, extraction and organization of different data from some databases. It was based a research question, and we was followed PRISMA guidelines to metodology. In the search phase, 115 articles were manually pre-selected, which then went through filtering processes to yield a total of 30 final articles present in this review. It was concluded that research with a holistic approach, multidisciplinary and comprehensive interventions, favor the quality of life in patients with ABI, and the nuuroplasticitu to the recovery of previous cognitive functions, lost after ABI, according to the different scientific articles analyzed. In addition, it was shown that there is not enough research on this topic in Latin America, and that most research comes from Europe and North America.1 introducción. -- 2. Justificación. -- 3. Objetivo. -- 3.1. Objetivo general. -- 3.2. Objetivos específicos. -- 4. Marco teórico. -- 4.1. Rehabilitación neuropsicológica. -- 4.2. Daño cerebral adquirido. -- 4.3. Calidad de vida. – 5. Metodología. -- 5.1. Criterios. -- 5.1.1. Criterios de inclusión. -- 5.1.2. Criterios de exclusión. -- 5.2. Fuentes de información. -- 5.3. Procesos de selección de estudios. -- 5.3.1. Primera fase de búsqueda. -- 5.3.2. Segunda fase de búsqueda. -- 5.3.3. Tercera fase de búsqueda. -- 5.4. Proceso de extracción de datos. -- 5.5. Flujograma. -- 5.6. Métodos de síntesis. -- 5.7. Análisis de datos. -- 6. Resultados. -- 6.1. Descripción estadística. -- 6.2. Análisis de Datos. -- 6.2.1 Rehabilitación cognitiva. -- 6.2.2 Rehabilitación neuropsicológica holística. -- 7. Conclusiones. -- 8. Discusión. -- 9. Referencias bibliográficas

Type I interferon enhances necroptosis of Salmonella Typhimurium-infected macrophages by impairing antioxidative stress responses

Salmonella enterica serovar Typhimurium exploits the host's type I interferon (IFN-I) response to induce receptor-interacting protein (RIP) kinase-mediated necroptosis in macrophages. However, the events that drive necroptosis execution downstream of IFN-I and RIP signaling remain elusive. In this study, we demonstrate that S. Typhimurium infection causes IFN-I-mediated up-regulation of the mitochondrial phosphatase Pgam5 through RIP3. Pgam5 subsequently interacts with Nrf2, which sequesters Nrf2 in the cytosol, thereby repressing the transcription of Nrf2-dependent antioxidative genes. The impaired ability to respond to S. Typhimurium-induced oxidative stress results in reactive oxygen species-mediated mitochondrial damage, energy depletion, transient induction of autophagy, and autophagic degradation of p62. Reduced p62 levels impair interaction of p62 with Keap1, which further decreases Nrf2 function and antioxidative responses to S. Typhimurium infection, eventually leading to cell death. Collectively, we identify impaired Nrf2-dependent redox homeostasis as an important mechanism that promotes cell death downstream of IFN-I and RIP3 signaling in S. Typhimurium-infected macrophages

Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy

During intracellular infections, autophagy significantly contributes to the elimination of pathogens, regulation of pro-inflammatory signaling, secretion of immune mediators and in coordinating the adaptive immune system. Intracellular pathogens such as S. Typhimurium have evolved mechanisms to circumvent autophagy. However, the regulatory mechanisms targeted by S. Typhimurium to modulate autophagy have not been fully resolved. Here we report that cytosolic energy loss during S. Typhimurium infection triggers transient activation of AMPK, an important checkpoint of mTOR activity and autophagy. The activation of AMPK is regulated by LKB1 in a cytosolic complex containing Sirt1 and LKB1, where Sirt1 is required for deacetylation and subsequent activation of LKB1. S. Typhimurium infection targets Sirt1, LKB1 and AMPK to lysosomes for rapid degradation resulting in the disruption of the AMPK-mediated regulation of mTOR and autophagy. The degradation of cytosolic Sirt1/LKB1/AMPK complex was not observed with two mutant strains of S. Typhimurium, Delta ssrB and.ssaV, both compromising the pathogenicity island 2 (SPI2). The results highlight virulence factor-dependent degradation of host cell proteins as a previously unrecognized strategy of S. Typhimurium to evade autophagy

Salmonella Typhimurium impairs glycolysis-mediated acidification of phagosomes to evade macrophage defense

Author summaryMacrophages undergo metabolic adaptations when they respond to invading pathogens. On the other hand, pathogens are also known to disrupt metabolic pathways to evade immune defense. In this study, we have employed metabolomics and transcriptomics to unravel that S. Typhimurium abrogates glycolysis and the modulators of glycolysis such as insulin-signaling. Downregulation of glycolysis leads to reduced acidification of phagosomes resulting in impaired bacterial clearance and antigen presentation. Furthermore, we provide evidence that induction of glycolysis facilitates v-ATPase assembly and the acidification of phagosomes by mobilizing aldolase to the v-ATPase complex. Our results highlight a previously unknown molecular link between metabolism and phagolysosome, which is targeted by S. Typhimurium to evade cell-autonomous defense. Regulation of cellular metabolism is now recognized as a crucial mechanism for the activation of innate and adaptive immune cells upon diverse extracellular stimuli. Macrophages, for instance, increase glycolysis upon stimulation with pathogen-associated molecular patterns (PAMPs). Conceivably, pathogens also counteract these metabolic changes for their own survival in the host. Despite this dynamic interplay in host-pathogen interactions, the role of immunometabolism in the context of intracellular bacterial infections is still unclear. Here, employing unbiased metabolomic and transcriptomic approaches, we investigated the role of metabolic adaptations of macrophages upon Salmonella enterica serovar Typhimurium (S. Typhimurium) infections. Importantly, our results suggest that S. Typhimurium abrogates glycolysis and its modulators such as insulin-signaling to impair macrophage defense. Mechanistically, glycolysis facilitates glycolytic enzyme aldolase A mediated v-ATPase assembly and the acidification of phagosomes which is critical for lysosomal degradation. Thus, impairment in the glycolytic machinery eventually leads to decreased bacterial clearance and antigen presentation in murine macrophages (BMDM). Collectively, our results highlight a vital molecular link between metabolic adaptation and phagosome maturation in macrophages, which is targeted by S. Typhimurium to evade cell-autonomous defense