Determinación y validación de una matriz genérica de ensayos para el desarrollo de revestimientos en piezas plásticas de automoción.

Treballs Finals de Grau d'Enginyeria Química, Facultat de Química, Universitat de Barcelona, Curs: 2013-2014, Tutors: Jose María Gutiérrez González, Carmen González Azón i Luís Martínez BarrioThe use of automotive plastic parts has greatly increased, and thus the need to develop new coatings to give them protective and visual characteristics. The requirements for the paintings in the automotive industry are evidenced by a list of assays that analyze the structural properties of the product once applied on the plastic component. Note that each automotive group needs a different testing methodology and requirements, and thus validating a product is difficult for several brands. The main objective of this work, identify and validate an assay generic matrix for the development of coatings on automotive plastic components, will be addressed first by the bibliographic study of required assays from the chosen companies to analyse. From this first study, a primary assay matrix containing all existing assays, will be obtained. Using selection criteria, the primary assay matrix will be reduced to one containing the assays to be performed. Once finished the assays, using a system that allows the comparison of the results obtained, the methodology of each assay will be analysed in order to see which is the most severe and therefore the most suitable to form part of an assay generic matrix. That allows performing the validation of a new product for several automotive brands. From the analysis of 8 assays with different methodology, which are common in 12 AkzoNobel's usual automotive market brands, each of them evaluates a different characteristic of the coating. A discriminated result has been obtained in 7 cases, therefore a generic restrictive validation has been generated for the development of new products for several brands

Preparación de materiales de referencia en el control anti-dopaje: estudio de las condiciones de liofilización.

Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2015, Tutors: Àngels Sahuquillo Estrugo, Rosa Ventura Alemany i Rosa Bergés CasasUrine reference materials used in interlaboratory comparison must be homogeneous and stable over time. The lyophilisation process is one of the methods applied when preparing urine reference materials for ensuring stability during distribution, transport and storage. Urine samples of 11- nor- Δ9- tetrahydrocannabinol- 9- carboxylic acid (THC-COOH), Benzoylecgonine (BE), Ethyl glucuronide/Ethyl sulphate (EtG/EtS) and ethanol (EtOH) were prepared from blank urine previously filtered. Samples were fortified with the adequate standard solutions and they were divided in aliquots. Aliquots stored at 4 ºC and lyophilized aliquots were used to evaluate the homogeneity of the samples. Others were frozen at -20 ºC and they were used as reference aliquots. In the first analysis, all prepared samples, except EtOH sample, were considered homogeneous. Concentration differences of THC-COOH and BE between lyophilized aliquots and reference aliquots were minimum (lower than 5 %). However, differences higher than 8 % were observed for EtG/EtS. If these differences were maintained constant in subsequence quantifications, they could be attributed to the evaporation of the analytes during the lyophilisation process. Results obtained for EtOH sample show a completely loss of the analyte due to its high volatility

Proporcionalidad en 1º de E.S.O.

El objetivo principal de este Trabajo Fin de Máster es exponer una secuencia didáctica para la enseñanza del objeto matemático de la Proporcionalidad en 1º de E.S.O

La Integral Definida

Es una propuesta didáctica sobre la enseñanza del objeto matemático "Integral Definida" en un curso de Bachillerato

Nucleolin promotes in vitro translation of feline calicivirus genomic RNA

AbstractFeline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into cells, but it binds to both ends of the FCV genomic RNA, and stimulates its translation in vitro. AGRO100, an aptamer that specifically binds and inactivates NCL, caused a strong reduction in FCV protein synthesis. This effect could be reversed by the addition of full-length NCL but not by a ΔrNCL, lacking the N-terminal domain. Consistent with this, FCV infection of CrFK cells stably expressing ΔrNCL led to a reduction in virus protein translation. These results suggest that NCL is part of the FCV RNA translational complex, and that the N-terminal part of the protein is required for efficient FCV replication

Differential effects of two virtual reality interventions: distraction versus pain control

There is evidence that virtual reality (VR) pain distraction is effective at improving pain-related outcomes. However, more research is needed to investigate VR environments with other pain-related goals. The main aim of this study was to compare the differential effects of two VR environments on a set of pain-related and cognitive variables during a cold pressor experiment. One of these environments aimed to distract attention away from pain (VRD), whereas the other was designed to enhance pain control (VRC). Participants were 77 psychology students, who were randomly assigned to one of the following three conditions during the cold pressor experiment: (a) VRD, (b) VRC, or (c) Non-VR (control condition). Data were collected regarding both pain-related variables (intensity, tolerance, threshold, time perception, and pain sensitivity range) and cognitive variables (self-efficacy and catastrophizing). Results showed that in comparison with the control condition, the VRC intervention significantly increased pain tolerance, the pain sensitivity range, and the degree of time underestimation. It also increased self-efficacy in tolerating pain and led to a reduction in reported helplessness. The VRD intervention significantly increased the pain threshold and pain tolerance in comparison with the control condition, but it did not affect any of the cognitive variables. Overall, the intervention designed to enhance control seems to have a greater effect on the cognitive variables assessed. Although these results need to be replicated in further studies, the findings suggest that the VRC intervention has considerable potential in terms of increasing self-efficacy and modifying the negative thoughts that commonly accompany pain problems