Indirect mitotic nondisjunction in Vicia faba and Chinese hamster cells

The hypothesis of indirect mitotic nondisjunction was tested in plant and mammalian cells. This hypothesis states that micronuclei derived from lagging chromosomes or chromatids are able to perform DNA synthesis and undergo mitotic condensation synchronously with main nuclei. Hence, as chromosomes, they can be moved to spindle poles together with the chromosomes of the main nuclei during mitosis. In that way chromosomes "lost" as micronuclei can be reincorporated in the main nuclei. In order to test this, both Vicia faba meristematic cells and cells of a Chinese hamster line (Cl-1) were treated with low doses of colchicine. Mitotic anomalies, micronuclei and cells with a polyploid or aneuploid karyotype were scored at different fixation times. A detailed analysis was performed on single chromosome misdistributions, as well as on micronuclei and cells with aneuploid karyotypes derived from single chromosome misdistributions. Indirect mitotic nondisjunction was shown to play a primary role in the origin of aneuploid karyotypes in Vicia faba, but not in Cl-1 cells

Primordial nucleosynthesis

Big Bang nucleosynthesis (BBN) describes the production of light nuclei in the early phases of the Universe. For this, precise knowledge of the cosmological parameters, such as the baryon density, as well as the cross section of the fusion reactions involved are needed. In general, the energies of interest for BBN are so low (E < 1MeV) that nuclear cross section measurements are practically unfeasible at the Earth’s surface. As of today, LUNA (Laboratory for Underground Nuclear Astrophysics) has been the only facility in the world available to perform direct measurements of small cross section in a very low background radiation. Owing to the background suppression provided by about 1400 meters of rock at the Laboratori Nazionali del Gran Sasso (LNGS), Italy, and to the high current offered by the LUNA accelerator, it has been possible to investigate cross sections at energies of interest for Big Bang nucleosynthesis using protons, 3He and alpha particles as projectiles. The main reaction studied in the past at LUNA is the 2H(4He, (Formula presented.))6Li. Its cross section was measured directly, for the first time, in the BBN energy range. Other processes like 2H(p, (Formula presented.))3He , 3He(2H, p)4He and 3He(4He, (Formula presented.))7Be were also studied at LUNA, thus enabling to reduce the uncertainty on the overall reaction rate and consequently on the determination of primordial abundances. The improvements on BBN due to the LUNA experimental data will be discussed and a perspective of future measurements will be outlined. © 2016, SIF, Springer-Verlag Berlin Heidelberg

First Measurement of the He3+He3-->He4+2p Cross Section down to the Lower Edge of the Solar Gamow Peak

We give the LUNA results on the cross section measurement of a key reaction of the proton-proton chain strongly affecting the calculated neutrino luminosity from the Sun: He3+He3-->He4+2p. Due to the cosmic ray suppression provided by the Gran Sasso underground laboratory it has been possible to measure the cross section down to the lower edge of the solar Gamow peak, i.e. as low as 16.5 keV centre of mass energy. The data clearly show the cross section increase due to the electron screening effect but they do not exhibit any evidence for a narrow resonance suggested to explain the observed solar neutrino flux.Comment: 5 pages, RevTeX, and 2 figures in PostScript Submitted for publicatio

Neutron-induced background by an alpha-beam incident on a deuterium gas target and its implications for the study of the 2H(alpha,gamma)6Li reaction at LUNA

The production of the stable isotope Li-6 in standard Big Bang nucleosynthesis has recently attracted much interest. Recent observations in metal-poor stars suggest that a cosmological Li-6 plateau may exist. If true, this plateau would come in addition to the well-known Spite plateau of Li-7 abundances and would point to a predominantly primordial origin of Li-6, contrary to the results of standard Big Bang nucleosynthesis calculations. Therefore, the nuclear physics underlying Big Bang Li-6 production must be revisited. The main production channel for Li-6 in the Big Bang is the 2H(alpha,gamma)6Li reaction. The present work reports on neutron-induced effects in a high-purity germanium detector that were encountered in a new study of this reaction. In the experiment, an {\alpha}-beam from the underground accelerator LUNA in Gran Sasso, Italy, and a windowless deuterium gas target are used. A low neutron flux is induced by energetic deuterons from elastic scattering and, subsequently, the 2H(d,n)3He reaction. Due to the ultra-low laboratory neutron background at LUNA, the effect of this weak flux of 2-3 MeV neutrons on well-shielded high-purity germanium detectors has been studied in detail. Data have been taken at 280 and 400 keV alpha-beam energy and for comparison also using an americium-beryllium neutron source.Comment: Submitted to EPJA; 13 pages, 8 figure

The 3He(alpha,gamma)7Be S-factor at solar energies: the prompt gamma experiment at LUNA

The 3He(alpha,gamma)7Be process is a key reaction in both Big-Bang nucleosynthesis and p-p chain of Hydrogen Burning in Stars. A new measurement of the 3He(alpha,gamma)7Be cross section has been performed at the INFN Gran Sasso underground laboratory by both the activation and the prompt gamma detection methods. The present work reports full details of the prompt gamma detection experiment, focusing on the determination of the systematic uncertainty. The final data, including activation measurements at LUNA, are compared with the results of the last generation experiments and two different theoretical models are used to obtain the S-factor at solar energies.Comment: Accepted for publication in Nucl. Phys.

Preparation and characterisation of isotopically enriched Ta2_2O5_5 targets for nuclear astrophysics studies

The direct measurement of reaction cross sections at astrophysical energies often requires the use of solid targets of known thickness, isotopic composition, and stoichiometry that are able to withstand high beam currents for extended periods of time. Here, we report on the production and characterisation of isotopically enriched Ta$_2$O$_5$ targets for the study of proton-induced reactions at the Laboratory for Underground Nuclear Astrophysics facility of the Laboratori Nazionali del Gran Sasso. The targets were prepared by anodisation of tantalum backings in enriched water (up to 66% in $^{17}$O and up to 96% in $^{18}$O). Special care was devoted to minimising the presence of any contaminants that could induce unwanted background reactions with the beam in the energy region of astrophysical interest. Results from target characterisation measurements are reported, and the conclusions for proton capture measurements with these targets are drawn.Comment: accepted to EPJ

Ultra-sensitive in-beam gamma-ray spectroscopy for nuclear astrophysics at LUNA

Ultra-sensitive in-beam gamma-ray spectroscopy studies for nuclear astrophysics are performed at the LUNA (Laboratory for Underground Nuclear Astrophysics) 400 kV accelerator, deep underground in Italy's Gran Sasso laboratory. By virtue of a specially constructed passive shield, the laboratory gamma-ray background for E_\gamma < 3 MeV at LUNA has been reduced to levels comparable to those experienced in dedicated offline underground gamma-counting setups. The gamma-ray background induced by an incident alpha-beam has been studied. The data are used to evaluate the feasibility of sensitive in-beam experiments at LUNA and, by extension, at similar proposed facilities.Comment: accepted, Eur. Phys. J.

First Direct Measurement of the ^{17}O(p,\gamma)^{18}F Reaction Cross-Section at Gamow Energies for Classical Novae

Classical novae are important contributors to the abundances of key isotopes, such as the radioactive ^{18}F, whose observation by satellite missions could provide constraints on nucleosynthesis models in novae. The ^{17}O(p,\gamma)^{18}F reaction plays a critical role in the synthesis of both oxygen and fluorine isotopes but its reaction rate is not well determined because of the lack of experimental data at energies relevant to novae explosions. In this study, the reaction cross section has been measured directly for the first time in a wide energy range Ecm = 200 - 370 keV appropriate to hydrogen burning in classical novae. In addition, the E=183 keV resonance strength, \omega \gamma=1.67\pm0.12 \mueV, has been measured with the highest precision to date. The uncertainty on the ^{17}O(p,\gamma)^{18}F reaction rate has been reduced by a factor of 4, thus leading to firmer constraints on accurate models of novae nucleosynthesis.Comment: accepted by Phys. Rev. Let

Measurement of 25Mg(p; gamma)26Al resonance strengths via gamma spectrometry

The COMPTEL instrument performed the first mapping of the 1.809 MeV photons in the Galaxy, triggering considerable interest in determing the sources of interstellar 26Al. The predicted 26Al is too low compared to the observation, for a better understanding more accurate rates for the 25Mg(p; gamma)26Al reaction are required. The 25Mg(p;gamma)26Al reaction has been investigated at the resonances at Er= 745; 418; 374; 304 keV at Ruhr-Universitat-Bochum using a Tandem accelerator and a 4piNaI detector. In addition the resonance at Er = 189 keV has been measured deep underground laboratory at Laboratori Nazionali del Gran Sasso, exploiting the strong suppression of cosmic background. This low resonance has been studied with the 400 kV LUNA accelerator and a HPGe detector. The preliminary results of the resonance strengths will be reported.Comment: Accepted for publication in Journal of Physics

The OPERA magnetic spectrometer

The OPERA neutrino oscillation experiment foresees the construction of two magnetized iron spectrometers located after the lead-nuclear emulsion targets. The magnet is made up of two vertical walls of rectangular cross section connected by return yokes. The particle trajectories are measured by high precision drift tubes located before and after the arms of the magnet. Moreover, the magnet steel is instrumented with Resistive Plate Chambers that ease pattern recognition and allow a calorimetric measurement of the hadronic showers. In this paper we review the construction of the spectrometers. In particular, we describe the results obtained from the magnet and RPC prototypes and the installation of the final apparatus at the Gran Sasso laboratories. We discuss the mechanical and magnetic properties of the steel and the techniques employed to calibrate the field in the bulk of the magnet. Moreover, results of the tests and issues concerning the mass production of the Resistive Plate Chambers are reported. Finally, the expected physics performance of the detector is described; estimates rely on numerical simulations and the outcome of the tests described above.Comment: 6 pages, 10 figures, presented at the 2003 IEEE-NSS conference, Portland, OR, USA, October 20-24, 200