Magnetic and superconducting instabilities in the periodic Anderson model: an RPA stud

We study the magnetic and superconducting instabilities of the periodic Anderson model with infinite Coulomb repulsion U in the random phase approximation. The Neel temperature and the superconducting critical temperature are obtained as functions of electronic density (chemical pressure) and hybridization V (pressure). It is found that close to the region where the system exhibits magnetic order the critical temperature T_c is much smaller than the Neel temperature, in qualitative agreement with some T_N/T_c ratios found for some heavy-fermion materials. In our study, all the magnetic and superconducting physical behaviour of the system has its origin in the fluctuating boson fields implementing the infinite on-site Coulomb repulsion among the f-electrons.Comment: 9 pages, 2 figure

In situ detection of fungal biofilms in a water distribution system, Alto do Céu, Recife, Brazil

Recent investigations on water microbiology have shown that most of the biomass present in the drinking water network is located at the pipe walls as biofilms. Biofilms are organized in highly efficient and stable ecosystems and can be viewed as a survival mechanism; this way of life can provide protection from chemical, biological or physical stresses. Moreover, biofilms play a major role in the accumulation, protection and dissemination of pathogens through water distribution systems. Although filamentous fungi are especially adapted for growth on surfaces, fungal water biofilms have received less attention when compared with bacterial biofilms, thus remaining a lack of information in this field. The use of microscopy techniques associated with image analyses has become a valuable tool for in situ studies. Fluorescent in situ hybridization (FISH) and fluorescent dyes are non-invasive and nondestructive techniques which provide information on cell morphology, metabolism and phylogeny. In this work, we aimed to detect filamentous fungal biofilm in a water distribution system using FISH (EUK516 and FUN1429 probes) and Calcofluor staining in replaced pipes. We also presented a sampler developed to study in situ fungal biofilms formation in water distribution system. Calcofluor staining was a rapid and easy method to detect filamentous biofilms on pipes surfaces. Additionally, FISH provided phylogenetic information by the detection of eukaryotic and fungal cells. Ours results contributed to demonstrate the presence of fungi in water biofilms and emphasised that fungi play an important role in water biofilms and microbial water quality

Biofilms from a Brazilian water distribution system include filamentous fungi

Filamentous fungi in drinking water can block water pipes, can cause organoleptic biodeterioration, and are a source of pathogens. There are increasing reports of the involvement of the organisms in biofilms. This present study describes a sampling device that can be inserted directly into pipes within water distribution systems, allowing biofilm formation in situ. Calcofluor White M2R staining and fluorescent in situ hybridization with morphological analyses using epifluorescent microscopy were used to analyse biofilms for filamentous fungi, permitting direct observation of the fungi. DAPI (4=,6-diamidino-2- phenylindole) was applied to detect bacteria. Filamentous fungi were detected in biofilms after 6 months on coupons exposed to raw water, decanted water and at the entrance of the water distribution system. Algae, yeast, and bacteria were also observed. The role of filamentous fungi requires further investigations.The authors acknowledge Companhia Pernambucana de Saneamento (COMPESA) for its support in making the work by H.M.B. Oliveira possible. V.M. de Siqueira is supported by the grant SFRH/BD/43719/2008 from Fundacao para a Ciencia e Tecnologia (FCT), Portugal

Filamentous fungi occurrence in free water and biofilms from drinking water storage tanks

In some regions of Brazil, especially where the water is scarce, drinking water is stored in water storage tanks. This practice gives the consumer the guarantee of available water. The water storage conditions such as the exposure to hot weather when the tanks are on rooftops allow the development of microorganisms and microbial biofilms which can deteriorate the water quality and increase the risk to human health [1,2]. This study describes the filamentous fungi (FF) detected in free water and biofilms in drinking water storage tanks in Recife - Pernambuco, Brazil. Five sampling times in triplicate were performed at two distinct points. Colony-forming units (CFU) of FF fungi were determined with 0.45 μm filtration membranes using peptone glucose rose Bengal agar (PGRBA). From the 30 samples analysed a total of 1136 CFU were obtained. The water biofilms were collected from samplers consisting of polyethylene coupons, previously installed in the reservoirs. These coupons were transferred to PGRBA plates and incubated using with the same conditions described for free FF. For the in situ detection of FF in biofilms the Calcofluor White staining technique was used. This procedure demonstrated FF forming biofilms on the surfaces of the coupons. Brazilian legislation does not define limits for FF in drinking water. However considering the potential risk of fungal contamination, the data obtained in this study will contribute to developing future quantitative and qualitative parameters for the presence of fungi in drinking water distribution systems in Brazil

Desenvolvimento de protótipo embarcado para automatização da medida do tempo de cozimento de feijão.

O presente trabalho propicia o fortalecimento do protocolo de intenções entre a Pontifícia Universidade Católica de Goiás ? PUC-GO e a Embrapa, na geração de benefícios sociais, envolvendo equipe multidisciplinar, que propõe a finalização e validação de um produto envolvendo o hardware e software para se obter a automatização dessa análise laboratorial.CONAFE

Filamentous fungi in drinking water tanks of a water supply system in Recife-PE, Brazil

Drinking water reservoirs are widely used in various building constructions in Brazil for as a source of potable water for users. However, they are susceptible to the growth of microorganisms such as fungi. From these, several negative factors may ensue, namely, unpleasant odors and flavours, pigments, biofilm formation and mycotoxins. This is caused fungal resistance to treatment and disinfection. Brazilian law and those of other countries offer no encouragement for the research of the species of fungi that cause these problems. There are no legal limits as to the presence in drinking water. This study assesses the occurrence of filamentous fungi in drinking water reservoirs connected to the Alto do Céu distribution system located in Recife, Pernambuco, Brazil. Four monthly collections were carried out, in triplicate, at two fixed points in the network, at the same treatment area, and another 2.0 km away from this site. For water physicochemical characterization at the time of collection, pH, temperature and free residual chlorine were analyzed. Filamentous fungi were quantified by filtration through cellulose nitrate membrane with a 0.45 μm porosity. All strains were grown in Petri dishes containing Peptone Glucose, Rose Bengal Agar (PGRBA) and were incubated at 30°C for 48h. Fungal occurrence was quantified as colony forming units (CFU) per litre. Twenty-four samples were analyzed, and these accounted for 807 CFU. Considering the potential for water spreading diseases, and the high toxicity of fungi into account it is intended that this work may prompt the creation of acceptable parameters as to the presence of fungi in drinking water networks

Filamentous fungi in biofilms of water tanks in a supply system in Recife-PE, Brazil

Fungi cause detrimental organoleptic effects in water, and their presence may facilitate the occurrence of infections, allergic reactions and harmful effects from mycotoxins. Despite the lack of knowledge on the biological structure of filamentous fungi in biofilms, it is known that they can contribute with bacteria, yeasts, protozoa and viruses. Hence, this study analyzed, in situ, filamentous fungi in biofilms found in drinking water reservoirs of the distribution network in Alto do Céu, Recife, Pernambuco, Brazil. Biofilms were collected, in triplicate from samplers consisting of a polyethylene plate (4.0cm x 1.5cm) established in drinking water reservoirs. Four monthly collections were carried out between August and November 2011 at two fixed points. Polyethylene samplers were taken to the laboratory and washed with sterile water and transferred to Petri dishes containing medium Peptone, Glucose, Rose Bengal Agar (PGRBA). The plates were incubated at 30°C for 48h. The occurrence of fungi was reported as CFU/L. Fifty three colony forming units of fungi were quantified. For water physicochemical characterization at the time of collection, pH, temperature and free residual chlorine were analyzed. For detection of biofilms, a combination of two fluorescent techniques was used: (A) Fluorescent “in situ” hybridization (FISH) using a rDNA universal probe EUK516 (ACCAGACTTGCCCTCC 5’-3’, MWG Biotech, Ebersberg, Germany) labeled with cyanine Cy3 at the 5’ end and (B) Calcofluor White M2R (CW). After FISH and CW, the samples were observed under an epifluorescent microscope. Fungal filaments which formed biofilms in the distribution network were observed establishing biofilm formation. These results confirm the presence and relevance of filamentous fungi in structuring biofilms

Developing social capital in implementing a complex intervention: a process evaluation of the early implementation of a suicide prevention intervention in four European countries

<p>Background: Variation in the implementation of complex multilevel interventions can impact on their delivery and outcomes. Few suicide prevention interventions, especially multilevel interventions, have included evaluation of both the process of implementation as well as outcomes. Such evaluation is essential for the replication of interventions, for interpreting and understanding outcomes, and for improving implementation science. This paper reports on a process evaluation of the early implementation stage of an optimised suicide prevention programme (OSPI-Europe) implemented in four European countries.</p> <p>Methods: The process analysis was conducted within the framework of a realist evaluation methodology, and involved case studies of the process of implementation in four European countries. Datasets include: repeated questionnaires to track progress of implementation including delivery of individual activities and their intensity; serial interviews and focus groups with stakeholder groups; and detailed observations at OSPI implementation team meetings.</p> <p>Results: Analysis of local contexts in each of the four countries revealed that the advisory group was a key mechanism that had a substantial impact on the ease of implementation of OSPI interventions, particularly on their ability to recruit to training interventions. However, simply recruiting representatives of key organisations into an advisory group is not sufficient to achieve impact on the delivery of interventions. In order to maximise the potential of high level ‘gatekeepers’, it is necessary to first transform them into OSPI stakeholders. Motivations for OSPI participation as a stakeholder included: personal affinity with the shared goals and target groups within OSPI; the complementary and participatory nature of OSPI that adds value to pre-existing suicide prevention initiatives; and reciprocal reward for participants through access to the extended network capacity that organisations could accrue for themselves and their organisations from participation in OSPI.</p> <p>Conclusions: Exploring the role of advisory groups and the meaning of participation for these participants revealed some key areas for best practice in implementation: careful planning of the composition of the advisory group to access target groups; the importance of establishing common goals; the importance of acknowledging and complementing existing experience and activity; and facilitating an equivalence of benefit from network participation.</p&gt