Study of Vitamin D Levels in Chronic Obstructive Pulmonary Disease Patients and Healthy Volunteers

INTRODUCTION: Vitamin D deficiency seems to be associated with pulmonary function detoriation. The present study was designed to investigate the relationship between serum vitamin D in patients with chronic obstructive pulmonary disease (COPD) and healthy volunteers. AIM AND OBJECTIVES: 1. To compare vitamin- D levels in COPD patients & healthy volunteers. 2. To analyse the level of vitamin-D levels in stable COPD pateints & in COPD patients with exacerbations. 3. To analyse the vitamin-D levels according the severity of COPD in patients. METHODOLOGY: A case control study with 50 cases and 50 controls are carried out during the period of February 2018 to June 2019 at Karpaga Vinayaga Institute of Medical Sciences. Confirmed cases of COPD as per the GOLD guidelines and Controls were subjected to estimation of serum Vitamin D level by CLIA method. RESULTS: The mean age of the COPD cases and Controls are 56.9 ± 7.90 and 53.84 ± 9.62 and there is slight preponderance for male gender (64%) for COPD. Hypovitaminosis D is present in 40% of COPD patients and 12% of controls. The mean Vitamin D level among COPD patients and Healthy volunteers in the present study are 32.21 ± 12.68 and 52.05 ± 1.99 respectively and it is very significant. The mean vitamin D level among grade I, II, III, IV COPD are 44.87±8.03, 34.64±9.45, 32.73±13.03, 14.87±1.65 respectively. COPD patients with <2 exacerbations per year had mean Vitamin D levels of 39.26±6.41 and COPD patients with ≥2 exacerbations per year had mean vitamin D level of 31.04±13.11. CONCLUSION: These findings indicate that there is significant decrease in Vitamin D level in COPD patients than among controls. Vitamin D levels correlate inversely with the increase in severity of COPD and number of exacerbations

Phase II Nonrandomized Comparative Open Clinical Evaluation on “Soothaga Vaayu” (Poly Cystic Ovarian Syndrome) treated with “Siddhar Yoga Therapy” and (Internal) “Attathi Chooranam”

The clinical study on Soothaga vaayu were carried out in Post Graduate Department of Siddhar Yoga Maruthuvam, Govt. Siddha medical college, attached to Arignar Anna Hospital of Indian Medicine, Chennai – 600 106 during the period of 2020 – 2022. The study population includes 40 patients. The clinical and statistical assessments were carried out on the basis of both Siddha and Modern aspects. Out of 40 patients with a comparative study on Group I –Attathi chooranam (internal) along with Siddhar yoga therapy has provided for 20 patients and Group II- Siddhar yoga therapy alone has provided for 20 patients were treated for this thesis work. The duration of treatment was fixed 90 days. The patients was monitored weekly once for 90 days. The results obtained from the studied and summarized. ❖ 45% of the patients were in the age group 15- 24 and 55% of the patients were in the age group 25 – 35. ❖ Majority of the patients (70%) were reported in their Vaatha kalam. ❖ According to this study the prevalence of PCOS was high (50%) among the students. ❖ Majority of the patients (55%) were from lower income group. ❖ 62.5% of the patients were married. ❖ All the patients were from Neithal thinai (100%) ❖ Most of the patients affected in Pinpani kaalam (50%) ❖ Most of the patients had vatha yakkai (30%) ❖ 90% of the patients consumed mixed diet. ❖ On considering BMI, 35% patients were obese, 35% patients were overweight and 30% of the patients had normal BMI before treatment. After treatment 65% of the patients improved to have normal BMI, 35% of the patients had overweight. ❖ Only 10% of the patients had family history of PCOS. ❖ In Poriyal therthal, Naaku (12.5%) and Kan (15%) were affected before treatment. After treatment with the trial drug all the patients Naaku and 12.5% of the patients Kan was improved. ❖ Regarding Kanmenthiriyangal, Karuvai was affected in all the patients before treatment and 90% of the patients improved after treatment with Attathi chooranam. Among the ten types of Vaatham, Abanan and Samanan were affected in all the patients, Vyanan affected in 2 patients and Devathathan affected in 3 patients before treatment. After treatment Abanan improved in all the patients, Vyanan improved in 1 patient, Samanan improved in 37 patients, and Devathathan improved in 2 patients. ❖ Among the five types of Pitham, Analaga pitham affected in 2 patient, Ranjaga pitham affected in 3 patients and Sathaga pitham affected in all the patients. After treatment all the patients Analaga pitham and Saathaga pitham were improved and Ranjaga pitham improved in 2 patients. ❖ Among the five types of Kapham, Santhigam was affected in all the patients before treatment. And after treatment all the patients were recovered. ❖ Among Udal thathukkal, Saaram and Suronitham were affected in all the patients before treatment. After treatment with the study drug Saaram and Suronitham came to normal in 90% of the patients. ❖ Considering Envagai thervu, 10% of the patients Naa, 15% of the patients Niram, 15.5% of the patients Vizhi and 10% of the patients Malam were affected before treatment. After treatment, 95% of the patients Naa, Niram, Vizhi and all the patients Malam were recovered. ❖ 37.5% of the patients had Pitha vaatha naadi, 27.5% of the patients had Vatha pitha naadi and 35% of the patients had Kapha pitha naadi before treatment. After treatment most of the patients (17.5%) have Vatha pitha naadi. ❖ In Neikuri only 40% of the patients had Vaatha neer before treatment. After treatment 55% of the patients had Vaatha neer. ❖ Bio – statistical analysis of the clinical trial reveals significant p values between before treatment and then after treatment among the variables regularity of menstruation, length of the cycle, duration of the cycle, low back pain, abdominal pain, hirsutism, FSH level, Serum cholesterol level and USG findings of both right and left ovary. ❖ Out of 40 patients 80% of the PCOS patients showed good outcome to treatment with Attathi chooranam. ❖ The clinical trial shows that significant improvement in clinical manifestations of Soothaga vaayu. ❖ The p-value is less than 0.05, reject the null hypothesis. That is there exist statistically significant difference between treatments (Method1 v/s Method2). The results obtained were found to be Attathi chooranam (Internal) along with Siddhar Yoga therapy is effective. No adverse reaction was found, the trail drugs. CONCLUSION: 40 patients with a comparative study on Group I –Attathi choornam (Internal) along with Siddhar yoga therapy has been provided for 20 patients and Group II – Siddhar yoga therapy alone has been provided for 20 patients were treated Soothaga vaayu (Poly cystic ovarian syndrome) for this dissertation work. ❖ The clinical study shows significant decrease in the symptoms of Soothaga vaayu (Poly cystic ovarian syndrome) the Attathi choornam (Internal) along with Siddhar yoga therapy gives a good confidence of management of Soothaga vaayu (Poly cystic ovarian syndrome) and economically very low cost. No contra indication noted in the clinical trial of treatment. ❖ Finally concluded the Attathi choornam (Internal) along with Siddhar yoga therapy is effective than Siddhar yoga therapy alone in the treatment of Soothaga vaayu (Poly cystic ovarian syndrome). For more results in future studies should be continued

Antibacterial and anticancer activity of trimetal nanoparticles

Medical field is faced with the major threat of antibiotic resisting bacteria and has turned to nanotechnology for both the drugs and their delivery. In the current study, the antibacterial and anti-cancer activities of La/Bi/Cu trimetalnanoparticles have been evaluated. They were found to decrease the cell viability of HOS cell lines in in-vitro analysis. La/Bi/Cu trimetalnanoparticles were also found to control growth of two Gram negative bacteria (Enterobacterclocae, Pseudomonas Vs1)and two Gram positive bacteria(Bacillus 1.3,Bacillus8.3)at different concentration (50-150µg/L).Based on the results, the nanoparticles can be used against human cancer and other infectious diseases