Rhizosphere microbial ecological characteristics of strawberry root rot

IntroductionStrawberry (Fragaria × ananassa Duch.) holds a preeminent position among small fruits globally due to its delectable fruits and significant economic value. However, strawberry cultivation is hampered by various plant diseases, hindering the sustainable development of the strawberry industry. The occurrence of plant diseases is closely linked to imbalance in rhizosphere microbial community structure.MethodsIn the present study, a systematic analysis of the differences and correlations among non-culturable microorganisms, cultivable microbial communities, and soil nutrients in rhizosphere soil, root surface soil, and non-rhizosphere soil of healthy and diseased strawberry plants affected by root rot was conducted. The goal was to explore the relationship between strawberry root rot occurrence and rhizosphere microbial community structure.ResultsAccording to the results, strawberry root rot altered microbial community diversity, influenced fungal community composition in strawberry roots, reduced microbial interaction network stability, and enriched more endophytic-phytopathogenic bacteria and saprophytic bacteria. In addition, the number of bacteria isolated from the root surface soil of diseased plants was significantly higher than that of healthy plants.DiscussionIn summary, the diseased strawberry plants changed microbial community diversity, fungal species composition, and enriched functional microorganisms significantly, in addition to reshaping the microbial co-occurrence network. The results provide a theoretical basis for revealing the microecological mechanism of strawberry root rot and the ecological prevention and control of strawberry root rot from a microbial ecology perspective

Plant resistance to tomato yellow leaf curl virus is enhanced by Bacillus amyloliquefaciens Ba13 through modulation of RNA interference

IntroductionTomato yellow leaf curl virus (TYLCV), which is a typical member of the genus Begomovirus, causes severe crop yield losses worldwide. RNA interference (RNAi) is an important antiviral defense mechanism in plants, but whether plant beneficial microbes used as biocontrol agents would modulate RNAi in defense against TYLCV remains unclear.MethodsHere, we employed whole-transcriptome, bisulfite, and small RNA sequencing to decipher the possible role of Bacillus amyloliquefaciens Ba13 as a bacterial biocontrol agent against TYLCV in RNAi modulation.ResultsPotted tomato plants were exposed to whiteflies for natural viral infection 14 days after bacterial inoculation. Compared with non-inoculated controls, the abundance of TYLCV gene in the leaves of inoculated plants decreased by 70.1% at 28 days post-infection, which mirrored the pattern observed for plant disease index. The expression of the ARGONAUTE family genes (e.g., AGO3, AGO4, AGO5, and AGO7) involved in antiviral defense markedly increased by 2.44–6.73-fold following bacterial inoculation. The methylation level at CpG site 228 (in the open reading frame region of the RNA interference suppressing gene AV2) and site 461 (in the open reading frame regions of AV1 and AV2) was 183.1 and 63.0% higher in inoculated plants than in non-inoculated controls, respectively. The abundances of 10 small interfering RNAs matched to the TYLCV genome were all reduced in inoculated plants, accompanied by enhancement of photosystem and auxin response pathways.DiscussionThe results indicate that the application of Ba. amyloliquefaciens Ba13 enhances plant resistance to TYLCV through RNAi modulation by upregulating RNAi-related gene expression and enhancing viral genome methylation

How fast could a proto-pulsar rotate?

According to two estimated relations between the initial period and the dynamo-generated magnetic dipole field of pulsars, we calculate the statistical distributions of pulsar initial periods. It is found that proto-pulsars are very likely to have rotation periods between 20 and 30 ms, and that most of the pulsars rotate initially at a period < 60 ms.Comment: submitted, or at http://vega.bac.pku.edu.cn/~rxxu/publications/index_P.ht

Precision Measurement of the Mass of the τ\tau Lepton

An energy scan near the $\tau$ pair production threshold has been performed using the BESIII detector. About $24$ pb$^{-1}$ of data, distributed over four scan points, was collected. This analysis is based on $\tau$ pair decays to $ee$, $e\mu$, $eh$, $\mu\mu$, $\mu h$, $hh$, $e\rho$, $\mu\rho$ and $\pi\rho$ final states, where $h$ denotes a charged $\pi$ or $K$. The mass of the $\tau$ lepton is measured from a maximum likelihood fit to the $\tau$ pair production cross section data to be $m_{\tau} = (1776.91\pm0.12 ^{+0.10}_{-0.13}$) MeV/$c^2$, which is currently the most precise value in a single measurement.Comment: 13 pages, 7 figure

Observation of a charged charmoniumlike structure in e+e−→(D∗Dˉ∗)±π∓e^+e^- \to (D^{*} \bar{D}^{*})^{\pm} \pi^\mp at s=4.26\sqrt{s}=4.26GeV

We study the process $e^+e^- \to (D^{*} \bar{D}^{*})^{\pm} \pi^\mp$ at a center-of-mass energy of 4.26GeV using a 827pb$^{-1}$ data sample obtained with the BESIII detector at the Beijing Electron Positron Collider. Based on a partial reconstruction technique, the Born cross section is measured to be $(137\pm9\pm15)$pb. We observe a structure near the $(D^{*} \bar{D}^{*})^{\pm}$ threshold in the $\pi^\mp$ recoil mass spectrum, which we denote as the $Z^{\pm}_c(4025)$. The measured mass and width of the structure are $(4026.3\pm2.6\pm3.7)$MeV/c$^2$ and $(24.8\pm5.6\pm7.7)$MeV, respectively. Its production ratio $\frac{\sigma(e^+e^-\to Z^{\pm}_c(4025)\pi^\mp \to (D^{*} \bar{D}^{*})^{\pm} \pi^\mp)}{\sigma(e^+e^-\to (D^{*} \bar{D}^{*})^{\pm} \pi^\mp)}$ is determined to be $0.65\pm0.09\pm0.06$. The first uncertainties are statistical and the second are systematic.Comment: 7 pages, 4 figures, 1 table; version accepted to be published in PR

Precision measurement of the D∗0D^{*0} decay branching fractions

Using 482 pb$^{-1}$ of data taken at $\sqrt{s}=4.009$ GeV, we measure the branching fractions of the decays of $D^{*0}$ into $D^0\pi^0$ and $D^0\gamma$ to be \BR(D^{*0} \to D^0\pi^0)=(65.5\pm 0.8\pm 0.5)% and \BR(D^{*0} \to D^0\gamma)=(34.5\pm 0.8\pm 0.5)% respectively, by assuming that the $D^{*0}$ decays only into these two modes. The ratio of the two branching fractions is \BR(D^{*0} \to D^0\pi^0)/\BR(D^{*0} \to D^0\gamma) =1.90\pm 0.07\pm 0.05, which is independent of the assumption made above. The first uncertainties are statistical and the second ones systematic. The precision is improved by a factor of three compared to the present world average values

Search for the Lepton Flavor Violation Process J/ψ→eμJ/\psi \to e\mu at BESIII

We search for the lepton-flavor-violating decay of the $J/\psi$ into an electron and a muon using $(225.3\pm2.8)\times 10^{6}$ $J/\psi$ events collected with the BESIII detector at the BEPCII collider. Four candidate events are found in the signal region, consistent with background expectations. An upper limit on the branching fraction of $\mathcal{B}(J/\psi \to e\mu)< 1.5 \times 10^{-7}$ (90% C.L.) is obtained

Measurement of yCPy_{CP} in D0−D‾0D^0-\overline{D}^0 oscillation using quantum correlations in e+e−→D0D‾0e^+e^-\to D^0\overline{D}^0 at s\sqrt{s} = 3.773\,GeV

We report a measurement of the parameter $y_{CP}$ in \ensuremath{D^0}\xspace-\ensuremath{\overline{D}^{0}}\xspace oscillations performed by taking advantage of quantum coherence between pairs of \ensuremath{D^0}\xspace \ensuremath{\overline{D}^{0}}\xspace mesons produced in $e^+e^-$ annihilations near threshold. In this work, doubly-tagged \ensuremath{D^0}\xspace \ensuremath{\overline{D}^{0}}\xspace events, where one $D$ decays to a $CP$ eigenstate and the other $D$ decays in a semileptonic mode, are reconstructed using a data sample of 2.92\,fb$^{-1}$ collected with the BESIII detector at the center-of-mass energy of $\sqrt{s}$ = 3.773\,GeV. We obtain $y_{CP} = (-2.0\pm1.3\pm0.7)\%$, where the first uncertainty is statistical and the second is systematic. This result is compatible with the current world average.Comment: 13 pages, 3 figure