The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria

Multilayers of Fluorinated Amphiphilic Polyions for Marine Fouling Prevention

Sequential layer-by-layer (LbL) deposition of polyelectrolytes followed by chemical cross-linking was investigated as a method to fabricate functional amphiphilic surfaces for marine biofouling prevention applications. A novel polyanion, grafted with amphiphilic perfluoroalkyl polyethylene glycol (fPEG) side chains, was synthesized and subsequently used to introduce amphiphilic character to the LbL film. The structure of the polyanion was confirmed by FTIR and NMR. Amphiphilicity of the film assembly was demonstrated by both water and hexadecane static contact angles. XPS studies of the cross-linked and annealed amphiphilic LbL films revealed the increased concentration of fPEG content at the film interface. In antifouling assays, the amphiphilic LbL films effectively prevented the adhesion of the marine bacterium Pseudomonas (NCIMB 2021)

Mod/Resc Parsimony Inference

We address in this paper a new computational biology problem that aims at understanding a mechanism that could potentially be used to genetically manipulate natural insect populations infected by inherited, intra-cellular parasitic bacteria. In this problem, that we denote by \textsc{Mod/Resc Parsimony Inference}, we are given a boolean matrix and the goal is to find two other boolean matrices with a minimum number of columns such that an appropriately defined operation on these matrices gives back the input. We show that this is formally equivalent to the \textsc{Bipartite Biclique Edge Cover} problem and derive some complexity results for our problem using this equivalence. We provide a new, fixed-parameter tractability approach for solving both that slightly improves upon a previously published algorithm for the \textsc{Bipartite Biclique Edge Cover}. Finally, we present experimental results where we applied some of our techniques to a real-life data set.Comment: 11 pages, 3 figure

Light meson mass dependence of the positive parity heavy-strange mesons

We calculate the masses of the resonances D_{s0}^*(2317) and D_{s1}(2460) as well as their bottom partners as bound states of a kaon and a D^*- and B^*-meson, respectively, in unitarized chiral perturbation theory at next-to-leading order. After fixing the parameters in the D_{s0}^*(2317) channel, the calculated mass for the D_{s1}(2460) is found in excellent agreement with experiment. The masses for the analogous states with a bottom quark are predicted to be M_{B^*_{s0}}=(5696\pm 40) MeV and M_{B_{s1}}=(5742\pm 40) MeV in reasonable agreement with previous analyses. In particular, we predict M_{B_{s1}}-M_{B_{s0}^*}=46\pm 1 MeV. We also explore the dependence of the states on the pion and kaon masses. We argue that the kaon mass dependence of a kaonic bound state should be almost linear with slope about unity. Such a dependence is specific to the assumed molecular nature of the states. We suggest to extract the kaon mass dependence of these states from lattice QCD calculations.Comment: 10 page

The Coupled Cluster Method in Hamiltonian Lattice Field Theory: SU(2) Glueballs

The glueball spectrum within the Hamiltonian formulation of lattice gauge theory (without fermions) is calculated for the gauge group SU(2) and for two spatial dimensions. The Hilbert space of gauge-invariant functions of the gauge field is generated by its parallel-transporters on closed paths along the links of the spatial lattice. The coupled cluster method is used to determine the spectrum of the Kogut-Susskind Hamiltonian in a truncated basis. The quality of the description is studied by computing results from various truncations, lattice regularisations and with an improved Hamiltonian. We find consistency for the mass ratio predictions within a scaling region where we obtain good agreement with standard lattice Monte Carlo results.Comment: 13 pages, 7 figure

Evidence for MBM_B and MCM_C phases in the morphotropic phase boundary region of (1−x)[Pb(Mg1/3Nb2/3)O3]−xPbTiO3(1-x)[Pb(Mg_{1/3}Nb_{2/3})O_3]-xPbTiO_3 : A Rietveld study

We present here the results of the room temperature dielectric constant measurements and Rietveld analysis of the powder x-ray diffraction data on $(1-x)[Pb(Mg_{1/3}Nb_{2/3})O_3]-xPbTiO_3$(PMN-$x$PT) in the composition range $0.20 \leq x \leq 0.45$ to show that the morphotropic phase boundary (MPB) region contains two monoclinic phases with space groups Cm (or $M_B$ type) and Pm (or $M_C$ type) stable in the composition ranges $0.27 \leq x \leq 0.30$ and $0.31 \leq x \leq 0.34$, respectively. The structure of PMN-$x$PT in the composition ranges $0 \leq x \leq$ 0.26, and $0.35 \leq x \leq1$ is found to be rhombohedral (R3m) and tetragonal (P4mm), respectively. These results are compared with the predictions of Vanderbilt & Cohen's theory.Comment: 20 pages, 11 pdf figure

Sudden switch of generalized Lieb-Robinson velocity in a transverse field Ising spin chain

The Lieb-Robinson theorem states that the speed at which the correlations between two distant nodes in a spin network can be built through local interactions has an upper bound, which is called the Lieb-Robinson velocity. Our central aim is to demonstrate how to observe the Lieb-Robinson velocity in an Ising spin chain with a strong transverse field. We adopt and compare four correlation measures for characterizing different types of correlations, which include correlation function, mutual information, quantum discord, and entanglement of formation. We prove that one of correlation functions shows a special behavior depending on the parity of the spin number. All the information-theoretical correlation measures demonstrate the existence of the Lieb-Robinson velocity. In particular, we find that there is a sudden switch of the Lieb-Robinson speed with the increasing of the number of spin

Phase diagram of the ferroelectric-relaxor (1-x)PbMg(1/3)Nb(2/3)O3-xPbTiO3

Synchrotron x-ray powder diffraction measurements have been performed on unpoled ceramic samples of (1-x)PbMg(1/3)Nb(2/3)O3-xPbTiO3 (PMN-xPT) with 30%<= x<= 39% as a function of temperature around the morphotropic phase boundary (MPB), which is the line separating the rhombohedral and tetragonal phases in the phase diagram. The experiments have revealed very interesting features previously unknown in this or related systems. The sharp and well-defined diffraction profiles observed at high and intermediate temperatures in the cubic and tetragonal phases, respectively, are in contrast to the broad features encountered at low temperatures. These peculiar characteristics, which are associated with the monoclinic phase of MC-type previously reported by Kiat et al and Singh et al., can only be interpreted as multiple coexisting structures with MC as the major component. An analysis of the diffraction profiles has allowed us to properly characterize the PMN-xPT phase diagram and to determine the stability region of the monoclinic phase, which extends from x= 31% to x= 37% at 20 K. The complex lansdcape of observed phases points to an energy balance between the different PMN-xPT phases which is intrinsically much more delicate than that of related systems such as PbZr(1-x)TixO3 or (1-x)PbZn(1/3)Nb(1/3)O3-xPbTiO3. These observations are in good accord with an optical study of x= 33% by Xu et al., who observed monoclinic domains with several different polar directions coexisting with rhombohedral domains, in the same single crystal.Comment: REVTeX4, 11 pages, 10 figures embedde