MicroRNA-612 regulates the proliferation and epithelial-to-mesenchymal transition of human colon cancer cells via G protein-coupled receptor 132 (GPR132)

Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and the mechanism involved. Methods: Expressions of miR-612 and GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using cell counting kit-8 (CCK8) and colony formation assays. Dual luciferase assay was used to determine the interaction between miR-612 and GPR132, while cell migration and invasion were measured by Transwell assay. Results: The expression levels of miR-612 in colon cancer tissues and cell lines were significantly down-regulated (p < 0.05). Overexpression of miR-612 in colon cancer cells led to significant inhibition of their proliferation and colony formation. Transwell assays revealed that miR-612 overexpression markedly stopped the migration, invasion and epithelial-to-mesenchymal transition. Conclusion: These results indicate that miR-612 exerts anti-cancer effect by suppressing the expression of GPR132 at the translational level. The in vitro tumor suppressive activity of miR-612 against colon cancer reveals its potential for the management of colon cancer

VisForum: A visual analysis system for exploring user groups in online forums

User grouping in asynchronous online forums is a common phenomenon nowadays. People with similar backgrounds or shared interests like to get together in group discussions. As tens of thousands of archived conversational posts accumulate, challenges emerge for forum administrators and analysts to effectively explore user groups in large-volume threads and gain meaningful insights into the hierarchical discussions. Identifying and comparing groups in discussion threads are nontrivial, since the number of users and posts increases with time and noises may hamper the detection of user groups. Researchers in data mining fields have proposed a large body of algorithms to explore user grouping. However, the mining result is not intuitive to understand and difficult for users to explore the details. To address these issues, we present VisForum, a visual analytic system allowing people to interactively explore user groups in a forum. We work closely with two educators who have released courses in Massive Open Online Courses (MOOC) platforms to compile a list of design goals to guide our design. Then, we design and implement a multi-coordinated interface as well as several novel glyphs, i.e., group glyph, user glyph, and set glyph, with different granularities. Accordingly, we propose the group Detecting 8 Sorting Algorithm to reduce noises in a collection of posts, and employ the concept of “forum-index” for users to identify high-impact forum members. Two case studies using real-world datasets demonstrate the usefulness of the system and the effectiveness of novel glyph designs. Furthermore, we conduct an in-lab user study to present the usability of VisForum.</jats:p

Seedling emergence and early growth of Chinese fir under different light levels and seed positions: implications for natural regeneration

Chinese fir, Cunninghamia lanceolata (Lamb.) Hook. (Taxodiaceae), is an evergreen conifer primarily distributed in southern China. This species exhibits very poor natural regeneration, possibly due to low light and a thick litter layer. To improve the understanding of the natural regeneration capacity of Chinese fir, in this study, we conducted a shade house experiment to determine the optimum light requirements and seed positions for seedling emergence and early growth. The experiment involved five light levels (100%, 60%, 40%, 15%, 5% of full sunlight) and four seed positions (1 cm beneath the soil surface without litter, on the soil surface without soil–seed contact, on the soil surface and covered with litter, and 1 cm beneath the soil surface and covered with litter). Seedling emergence was highest at 5%–15% sunlight, whereas seedling height, root length, root mass, stem mass, leaf mass, and total mass were highest at 60% sunlight. For each light level, seed position significantly affected emergence and growth. The above-litter position inhibited seedling emergence and survival, while the below-litter position favored seedling emergence and early growth, particularly under high light levels. Based on these results, to enhance natural regeneration of Chinese fir, we recommend periodical thinning to increase light into the understory after successful seedling emergence. We also recommend sowing seeds deeper into the litter to improve soil contact and moisture conditions

Intelligent-Reflecting-Surface-Assisted UAV Communications for 6G Networks

In 6th-Generation (6G) mobile networks, Intelligent Reflective Surfaces (IRSs) and Unmanned Aerial Vehicles (UAVs) have emerged as promising technologies to address the coverage difficulties and resource constraints faced by terrestrial networks. UAVs, with their mobility and low costs, offer diverse connectivity options for mobile users and a novel deployment paradigm for 6G networks. However, the limited battery capacity of UAVs, dynamic and unpredictable channel environments, and communication resource constraints result in poor performance of traditional UAV-based networks. IRSs can not only reconstruct the wireless environment in a unique way, but also achieve wireless network relay in a cost-effective manner. Hence, it receives significant attention as a promising solution to solve the above challenges. In this article, we conduct a comprehensive survey on IRS-assisted UAV communications for 6G networks. First, primary issues, key technologies, and application scenarios of IRS-assisted UAV communications for 6G networks are introduced. Then, we put forward specific solutions to the issues of IRS-assisted UAV communications. Finally, we discuss some open issues and future research directions to guide researchers in related fields

Responses of Chinese fir and Schima superba seedlings to light gradients: Implications for the restoration of mixed broadleaf-conifer forests from Chinese fir monocultures

Although Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) plantations are widely grown for timber production in southern China, they have low biodiversity and provide limited ecosystem services. To address this problem, C. lanceolata are increasingly mixed with broadleaf Schima superba Gardn. & Champ. (Theaceae). The success of these mixed plantations relies on introducing each species in the appropriate sequence, which requires understanding how tree species respond to light variations. We therefore compared S. superba and C. lanceolata seedling light tolerance in shaded houses under five light gradients (5%, 15%, 40%, 60%, and 100% sunlight). Our findings showed that S. superba seedlings exhibited greater net height increment (ΔHt), net diameter growth (ΔDia), leaf area, root mass, stem mass, leaf mass, and total mass under low light conditions (15% sunlight). However, as sunlight increased, these growth variables became higher in C. lanceolata seedlings. With more sunlight, both species experienced a drop in height to diameter ratio (HDR), and specific leaf area (SLA), but an elevated root to shoot ratio. Additionally, under the same light levels, S. superba seedlings exhibited greater leaf area and root to shoot ratio than C. lanceolata seedlings. Our results suggested that S. superba might be more suitable for underplanting beneath a heavy canopy due to its shade-tolerant traits. In contrast, C. lanceolata was less shade-tolerant, having an optimum seedling growth under full sunlight. These findings suggest that underplanting S. superba seedlings in C. lanceolata monoculture plantation (i.e., underplanting regeneration approach) could be a better silvicultural alternative than simultaneously planting both seedlings

Neuroprotective effects of etanercept on diabetic retinopathy via regulation of the TNF-α/NF-κB signaling pathway

Purpose: To study the influence of etanercept on diabetic retinopathy in rats via tumor necrosis factor alpha (TNF-α)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. Methods: Thirty-six Sprague-Dawley (SD) rats were randomly divided into normal, model and etanercept groups. The expression of Caspase-3 was determined by immunohistochemistry, while the relative protein and mRNA expression levels of TNF-α and NF-κB were determined by Western blotting and quantitative polymerase chain reaction, respectively. Besides, the contents of TNF-α and interleukin-1 beta (IL-1β) were evaluated using enzyme-linked immunosorbent assay (ELISA), while cell apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). Results: Immunohistochemical studies showed that the mean optical density of tissues positive for caspase-3 in both the model and etanercept groups were significantly higher than in the normal group (p &lt; 0.05), while the mean optical density in the etanercept group was significantly lower than that in the model group (p &lt; 0.05). The protein expression levels of TNF-α and NF-κB in the etanercept group were significantly lower than those in the model group (p &lt; 0.05). Furthermore, mRNA expressions of TNF-α and NF-κB declined in the etanercept group (p &lt; 0.05); in addition, TNF-α, and IL-1β levels in the etanercept group were lower than in the model group (p &lt; 0.05). Cell apoptosis in the etanercept group was also lower than in the model group. Conclusion: Etanercept suppresses TNF-α/NF-κB signaling pathway thereby repressing inflammation and cell apoptosis in diabetic retinopathy rats. Therefore, etenercept’s neuroprotective effect may potentially be useful in developing a suitable therapy for diabetic neuropathy

Non-invasive brain stimulation associated mirror therapy for upper-limb rehabilitation after stroke: Systematic review and meta-analysis of randomized clinical trials

BackgroundNon-invasive brain stimulation (NIBS) techniques and mirror therapy (MT) are promising rehabilitation measures for stroke. While the combination of MT and NIBS has been employed for post-stroke upper limb motor functional rehabilitation, its effectiveness has not been examined.ObjectiveThis study aimed to evaluate the effectiveness of combined MT and NIBS in the recovery of upper limb motor function in stroke patients.MethodsThe search was carried out in PubMed, EMBASE, Cochrane Library, Web of Science, Science Direct, CNKI, WANFANG and VIP until December 2021. Randomized clinical trials (RCTs) comparing MT or NIBS alone with the combination of NIBS and MT in improving upper extremity motor recovery after stroke were selected. A meta-analysis was performed to calculate the mean differences (MD) or the standard mean differences (SMD) and 95% confidence intervals (CI) with random-effect models. Subgroup analyses were also conducted according to the types of control group, the types of NIBS, stimulation timing and phase poststroke.ResultsA total of 12 articles, including 17 studies with 628 patients, were reviewed in the meta-analysis. In comparison with MT or NIBS alone, the combined group significantly improved body structure and function (MD = 5.97; 95% CI: 5.01–6.93; P &lt; 0.05), activity levels (SMD = 0.82; 95% CI 0.61–1.02; P &lt; 0.05). For cortical excitability, the motor evoked potential cortical latency (SMD = −1.05; 95% CI:−1.57–−0.52; P &lt; 0.05) and the central motor conduction time (SMD=-1.31 95% CI:−2.02-−0.61; P &lt; 0.05) of the combined group were significantly shortened. A non-significant homogeneous summary effect size was found for MEP amplitude (SMD = 0.47; 95%CI = −0.29 to 1.23; P = 0.23). Subgroup analysis showed that there is an interaction between the stimulation sequence and the combined treatment effect.ConclusionIn this meta-analysis of randomized clinical trials, in comparison to the control groups, MT combined with NIBS promoted the recovery of upper extremity motor function after stroke, which was reflected in the analysis of body structure and function, activity levels, and cortical excitability.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42022304455

Positive correlation between the expression of hEag1 and HIF-1α in breast cancers: An observational study

Objectives: To explore the expression patterns of Eag1 (ether á go-go 1) and HIF-1α (hypoxia-inducible factor 1α) in a cohort of patients with breast cancer. Setting: Department of general surgery in an upper first-class hospital in Xi\u27an, China. Participants: A total of 112 female Han Chinese patients with a diagnosis of invasive ductal carcinoma were included. Patients with main internal diseases, such as cardiovascular, endocrine, gastroenterological, haematological, infectious diseases, etc, were excluded. Primary and secondary outcome measures: Expression profiles of Eag1 and HIF-1α. Results: Eag1 and HIF-1α were overexpressed in the tumour tissues compared with the pair-matched control tissues, p=0.002 and \u3c0.001, respectively. The expression of Eag1 and HIF-1α was negatively correlated with tumour size, p=0.032 and p=0.025, respectively, and lymph node status (p=0.040, p=0.032, respectively). The coexpression of Eag1 and HIF-1α was correlated with tumour size ( p=0.012), lymph node status (p=0.027) and tumour stage (p=0.036). HIF-1α has a strong correlation with hEag1 expression (κ=0.731, p\u3c0.001). Conclusions: HIF-1á expression has a strong correlation with hEag1 expression. We are the first to attempt to explore the correlation at the population level

Simultaneous Ni Doping at Atom Scale in Ceria and Assembling into Well-Defined Lotuslike Structure for Enhanced Catalytic Performance

Oxide materials with redox capability have attracted worldwide attentions in many applications. Introducing defects into crystal lattice is an effective method to modify and optimize redox capability of oxides as well as their catalytic performance. However, the relationship between intrinsic characteristics of defects and properties of oxides has been rarely reported. Herein, we report a facile strategy to introduce defects by doping a small amount of Ni atoms (∼1.8 at. %) into ceria lattice at atomic level through the effect of microstructure of crystal on the redox property of ceria. Amazingly, a small amount of single Ni atom-doped ceria has formed a homogeneous solid solution with uniform lotuslike morphology. It performs an outstanding catalytic performance of a reduced T50 of CO oxidation at 230 °C, which is 135 °C lower than that of pure CeO2 (365 °C). This is largely attributed to defects such as lattice distortion, crystal defects and elastic strain induced by Ni dopants. The DFT calculation has revealed that the electron density distribution of oxygen ions near Ni dopant, the reduced formation energy of oxygen vacancy originated from local chemical effect caused by local distortion after Ni doping. These differences have a great effect on increasing the concentration of oxygen vacancies and enhancing the migration of lattice oxygen from bulk to a surface which is closely related to optimized redox properties. As a result, oxygen storage capacity and the associated catalytic reactivity has been largely increased. We have clearly demonstrated the change of crystal lattice and the charge distribution effectively modify its chemical and physical properties at the atomic scale

Effect of Pyrolysis Temperature on the Characterisation of Dissolved Organic Matter from Pyroligneous Acid

Dissolved organic matter (DOM) greatly influences the transformation of nutrients and pollutants in the environment. To investigate the effects of pyrolysis temperatures on the composition and evolution of pyroligneous acid (PA)-derived DOM, DOM solutions extracted from a series of PA derived from eucalyptus at five pyrolysis temperature ranges (240–420 °C) were analysed with Fourier transform infrared spectroscopy, gas chromatography–mass spectroscopy, and fluorescence spectroscopy. Results showed that the dissolved organic carbon content sharply increased (p 370 °C). The results of two-dimensional correlation spectroscopic analysis suggested that with increasing pyrolysis temperatures, the humic-acid-like substances became more sensitive than other fluorescent components. This study provides valuable information on the characteristic evolution of PA-derived DOM