4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues

Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the same sample

Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation

In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period

Botrytis species on bulb crops

Abstract. A number of Botrytis species are pathogens of bulb crops. Botrytis squamosa (teleomorph=Botrytotinia squamosa) causal agent of botrytis leaf blight and B. allii the causal agent of botrytis neck rotare two of the most important fungal diseases of onion. The taxonomics of several of the neck rotpathogens of onion have been revised on the basis of recent molecular sequence analysis studies. B. allii,B. aclada, and B. byssoidea are now recognized as distinct species causing neck rot diseases of onion. B.cinerea is also pathogenic on onion, primarily causing botrytis brown stain on onion bulbs. B. tulipae, B.elliptica, and B. gladiolorum are important pathogens of flower bulbs and are the causal agents of leafblight in tulip, lily, and gladiolus, respectively. Leaf blight in the major flower bulb crops is called ‘fire’referring to the fire-like symptoms occurring on the leaves of flower bulb plants when epidemics occur inproduction fields. In both the onion and flower bulb production systems chemicals are still heavily reliedupon to control the major diseases, however, alternative disease management systems also are used andundoubtedly will become increasingly important in controlling the diseases. Infected plants and colonizedplant debris are considered important sources of inoculum for B. squamosa, B. tulipae, and B. elliptica,particularly when sclerotia are formed. Sclerotia of B. squamosa serve as the source of conidia, as well asapothecia producing ascospores, in onion production areas in New York. The primary inoculum sourcesof B. allii and B. gladiolorum are believed to be infested seed and infected corms, respectively