Anti-fungal bandages containing cinnamon extract

© 2019 The Authors. International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd.Cinnamon-containing polycaprolactone (PCL) bandages were produced by pressurised gyration and their anti-fungal activities against Candida albicans were investigated. It was found that by preparing and spinning polymer solutions of cinnamon with PCL, fibres capable of inhibiting fungal growth could be produced, as observed in disk diffusion tests for anti-fungal susceptibility. Fascinatingly, compared with raw cinnamon powder, the novel cinnamon-loaded fibres had outstanding long-term activity. The results presented here are very promising and may indeed accelerate a new era of using completely natural materials in biomedical applications, especially in wound healing.Peer reviewe

Fabrication and optimization of 3D printed gelatin methacryloyl microneedle arrays based on vat photopolymerization

Microneedles (MNs) are micrometer-sized arrays that can penetrate the skin in a minimally invasive manner; these devices offer tremendous potential for the transdermal delivery of therapeutic molecules. Although there are many conventional techniques for manufacturing MNs, most of them are complicated and can only fabricate MNs with specific geometries, which restricts the ability to adjust the performance of the MNs. Herein, we present the fabrication of gelatin methacryloyl (GelMA) MN arrays using the vat photopolymerization 3D printing technique. This technique allows for the fabrication of high-resolution and smooth surface MNs with desired geometries. The existence of methacryloyl groups bonded to the GelMA was verified by 1H NMR and FTIR analysis. To examine the effects of varying needle heights (1000, 750, and 500 µm) and exposure times (30, 50, and 70 s) on GelMA MNs, the height, tip radius, and angle of the needles were measured; their morphological and mechanical properties were also characterized. It was observed that as the exposure time increased, the height of the MNs increased; moreover, sharper tips were obtained and tip angles decreased. In addition, GelMA MNs exhibited good mechanical performance with no breakage up to 0.3 mm displacement. These results indicate that 3D printed GelMA MNs have great potential for transdermal delivery of various therapeutics

Osteoblastic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth by Probiotic Hydroxyapatite

Objective: Multipotent cells derived from human exfoliated deciduous teeth (SHED) possess the ability to differentiateinto various cell types, including osteoblasts. This study aims to simulate the growth induction and osteogenicdifferentiation of SHED cells using probiotics and their resultant biomaterials.Materials and Methods: This experimental study proceeded in two stages. Initially, we evaluated the effect ofautoclaved nutrient agar (NA) grown probiotic Bacillus coagulans (B. coagulans) on the SHED and MG-63 cell lines.Subsequently, probiotics grown on the Pikovskaya plus urea (PVKU) medium and their synthesised hydroxyapatite (HA)were identified using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), andFourier transform infrared spectroscopy (FTIR), and then used to stimulate growth and osteogenic differentiation of theSHED cell line. Osteoblast cell differentiation was assessed by morphological changes, the alkaline phosphatase (ALP)assay, and alizarin red staining.Results: There was a substantial increase in SHED cell growth of about 14 and 33% due to probiotics grown on NAand PVKU medium, respectively. The PVKU grown probiotics enhanced growth and induced stem cell differentiationdue to HA content. Evidence of this differentiation was seen in the morphological shift from spindle to osteocyte-shapedcells after five days of incubation, an increase in ALP level over 21 days, and detection of intracellular calcium depositsthrough alizarin red staining-all indicative of osteoblast cell development.Conclusion: The osteogenic differentiation process in stem cells, improved by the nano-HA-containing byproducts ofprobiotic bacteria in the PVKU medium, represents a promising pathway for leveraging beneficial bacteria and theirsynthesised biomaterials in tissue engineering

Fabrication of ethosuximide loaded alginate/polyethylene oxide scaffolds for epilepsy research using 3D-printing method

Epilepsy is a medical condition that causes seizures and impairs the mental and physical activities of patients. Unfortunately, over one-third of patients do not receive adequate relief from oral Antiepileptic Drugs (AEDs) and continue to experience seizures. In addition to that, long term usage of Antiepileptic Drugs can cause a range of side effects. To overcome this problem, the precision of 3D printing technology is combined with the controlled release capabilities of biodegradable polymers, allowing for tailored and localized AED delivery to specific seizure sites. As a result of this novel technique, therapeutic outcomes can be enhanced, side effects of AEDs are minimized, and patient-specific dosage forms can be created. This study focused on the use of ethosuximide, an antiepileptic drug, at different concentrations (10, 13, and 15 mg) loaded into 3D-printed sodium alginate and polyethylene oxide scaffolds. The scaffolds contained varying concentrations (0.25%, 0.50%, and 0.75% w/v) and had varying pores created by 3D patterning sizes from 159.86 ± 19.9 µm to 240.29 ± 10.7 µm to optimize the releasing system for an intracranial administration. The addition of PEO changed the Tg and Tm temperatures from 65°C to 69°C and from 262°C to 267°C, respectively. Cytotoxicity assays using the human neuroblastoma cell line (SH-SY5Y) showed that cell metabolic activity reached 130% after 168 h, allowing the cells to develop into mature neural cells. In vitro testing demonstrated sustained ethosuximide release lasting 2 hours despite crosslinking with 3% CaCl2. The workpaves the way for the use of ethosuximide -loaded scaffolds for treating epilepsy

3D Propolis-Sodium Alginate Scaffolds: Influence on Structural Parameters, Release Mechanisms, Cell Cytotoxicity and Antibacterial Activity

FEN-C-YLP-130319-0065 BAPKO Project. UID/CTM/50025/2019In this study, the main aim was to fabricate propolis (Ps)-containing wound dressing patches using 3D printing technology. Different combinations and structures of propolis (Ps)-incorporated sodium alginate (SA) scaffolds were developed. The morphological studies showed that the porosity of developed scaffolds was optimized when 20% (v/v) of Ps was added to the solution. The pore sizes decreased by increasing Ps concentration up to a certain level due to its adhesive properties. The mechanical, swelling-degradation (weight loss) behaviors, and Ps release kinetics were highlighted for the scaffold stability. An antimicrobial assay was employed to test and screen antimicrobial behavior of Ps against Escherichia coli and Staphylococcus aureus strains. The results show that the Ps-added scaffolds have an excellent antibacterial activity because of Ps compounds. An in vitro cytotoxicity test was also applied on the scaffold by using the extract method on the human dermal fibroblasts (HFFF2) cell line. The 3D-printed SA-Ps scaffolds are very useful structures for wound dressing applications.publishersversionpublishe

Macromol. Mater. Eng. 3/2018

Bacterial cellulose blended polymeric fibrous bandages made in a novel way, from a solution subjected to gyration under pressure to directly weave the bandages. The products show cellular attraction, mechanical and swelling properties in preliminary tests and heralds a very promising new route for the manufacture of wound care bandages. This is reported by Esra Altun, Mehmet Onur Aydogdu, Fatma Koc, Maryam Crabbe‐Mann, Francis Brako, Rupy Kaur‐Matharu, Gunes Ozen, Serap Erdem Kuruca, Ursula Edirisinghe, Oguzhan Gunduz, and Mohan Edirisinghein

Novel Making of Bacterial Cellulose Blended Polymeric Fiber Bandages

Bacterial cellulose (BC) is a very promising biological material. However, at present its utilization is limited by difficulties in shape forming it. In this Communication, it is shown how this can be overcome by blending it with poly(methylmethacrylate) (PMMA) polymer. BC:PMMA fibers are produced by pressurized gyration of blended BC:PMMA solutions. Subsequently, BC:PMMA bandage‐like scaffolds are generated with different blends. The products are investigated to determine their morphological and chemical features. Cell culture and proliferation tests are performed to obtain information on biocompatibility of the scaffolds