44 research outputs found
Development of novel Cu(I) compounds with vitamin B1 derivative and their potential application as anticancer drugs
The synthesis and crystal structure of two new copper(I) compounds with molecular formula of {Cu4[(”3-thiocrome)2Cl4]â2(H2O)}n 1, and Cu2[(”2-thiocrome)2Cl2] 2 are reported. The crystal structure of compounds 1 and 2 are solved by single crystal X-ray diffraction. The reaction of Cu(II) with thiamine chloride in water at room temperature produces Cu(I) thiochrome compounds 1 and 2. Compound 1 shows a 1D chain structure based on the linkage of two crystallographic different copper centers and thiochrome ligand through the N(1), N(2) and N(3) nitrogen atoms. Compound 2 is a 0D dimeric copper structure assembled by two thiochrome ligands. For both compounds, the copper(I) centers exhibits a distorted trigonal pyramidal geometry. The antitumor capacity of both compounds was tested in vitro against a human cancer cell line, the colorectal adenocarcinoma (Caco-2) cell line, by determining their effect on cell viability. The two compounds showed similar IC50 values, and were slightly more potent than cisplatin, against the same cell line.publishe
Locking the GFP Fluorophore to Enhance Its Emission Intensity
Funding Information: Thanks are due to the University of Aveiro, FCT/MEC, Centro 2020 and Portugal2020, the COMPETE program, and the European Union (FEDER program) via the financial support to the LAQV-REQUIMTE (UIDB/50006/2020 and UIDP/50006/2020), to the CICECO-Aveiro Institute of Materials (UID/CTM/50011/2019, UIDB/50011/2020 and UIDP/50011/2020), financed by national funds through the FCT/MCTES, to the Portuguese NMR Network. SG is supported by national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. JRMF. Thanks FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for her PhD grant (UI/BD/151272/2021). Publisher Copyright: © 2022 by the authors.The Green Fluorescent Protein (GFP) and its analogues have been widely used as fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the fluorescence of the GFP is not emissive when isolated in solution, outside the protein environment. The most accepted explanation is that the quenching of the fluorescence results from the rotation of the arylâalkene bond and from the Z/E isomerization. Over the years, many efforts have been performed to block these torsional rotations, mimicking the environment inside the protein ÎČ-barrel, to restore the emission intensity. Molecule rigidification through chemical modifications or complexation, or through crystallization, is one of the strategies used. This review presents an overview of the strategies developed to achieve highly emissive GFP chromophore by hindering the torsional rotations.publishersversionpublishe
Synthesis and luminescence properties of analogues of the green fluorescent protein chromophore
The green fluorescent protein (GFP) is extensively used as a biomarker for fluorescence biological imaging. The chromophore in GFP is only fluorescent when confined into the ÎČâbarrel of the protein. Similarly, synthetic analogues of the fluorophore of GFP are usually non-emissive in solution, due to free rotation around the aryl-alkene bond and (Z/E)-isomerization of the double bond. Here, the synthesis and characterization of three analogues of the fluorophore of GFP are reported. The introduction of more electron donating substituents induces a red-shift in the absorption and emission. The fluorophores are more emissive in the solid state than in solution, and a study of their crystal structure reveals that the (Z/E)-isomerization is efficiently blocked in the crystals.publishe
Synthesis of Unsymmetrical Methylenebisphenol Derivatives
A simple and efficient route towards unsymmetrical methylenebisphenol derivatives is reported. This straightforward strategy avoids the use of harmful or dangerous chemicals, allowing the synthesis of highly functionalized bisphenyls with no need of protecting groups. The alkylation of the phenyl ring is selective for the para position of the hydroxyl substituent. All methylenebisphenols were obtained in a completely regioselective manner and isolated in high yields
Dimethyl 5,5âČ-methylÂenebis(2-hyÂdroxyÂbenzoate)
In the title compound, C17H16O6, the two methyl salicylate moieties are related by crystallographic twofold rotational symmetry with the two benzene rings close to being perpendicular [inter-ring dihedral angle = 86.6â
(8)°]. IntraÂmolecular phenolic OâHâŻO hydrogen bonds with carboxyl O-atom acceptors are present, with these groups also involved in centrosymmetric cyclic interÂmolecular OâHâŻO hydrogen-bonding associations [graph set R
2
2(4)], giving infinite chains extending across (101)
Steroidâquinoline hybrids for disruption and reversion of protein aggregation processes
Reversing protein aggregation within cells may be an
important tool to fight protein-misfolding disorders such as Alzheimerâs,
Parkinsonâs, and cardiovascular diseases. Here we report the design and
synthesis of a family of steroidâquinoline hybrid compounds based on
the framework combination approach. This set of hybrid compounds
effectively inhibited AÎČ1â42 self-aggregation in vitro by delaying the
exponential growth phase and/or reducing the quantity of fibrils in the
steady state. Their disaggregation efficacy was further demonstrated
against preaggregated AÎČ1â42 peptides in cellular assays upon their
endocytosis by neuroblastoma cells, as they reverted both the number
and the average area of fibrils back to basal levels. The antiaggregation
effect of these hybrids was further tested and demonstrated in a cellular
model of general protein aggregation expressing a protein aggregation fluorescent sensor. Together, our results show that the new
cholesterolâquinoline hybrids possess wide and marked disaggregation capacities and are therefore promising templates for the
development of new drugs to deal with conformational disorders.Thanks are due to the University of Aveiro, FCT/MEC,
Centro 2020 and Portugal2020, the COMPETE Program, and
the European Union (FEDER Program) via the financial
support to the research units LAQV-REQUIMTE (UIDB/50006/2020), IBiMED (UID/BIM/04501/2019) and CICECO-
Aveiro Institute of Materials (UID/CTM/50011/2019),
financed by national funds through the FCT/MCTES, to the
Portuguese NMR Network, to the ThiMES Project (POCI-01-
0145-FEDER-016630), and to the PAGE Project âProtein
Aggregation Across the Lifespanâ (CENTRO-01-0145-
FEDER-000003), including postdoctoral grants to H.M.T.A.
(BPD/UI98/4861/2017) and R.N.d.S. (BPD/UI98/6327/2018). M.P. was supported by Ph.D. Grant SFRH/BD/135655/2018. A.R.S. and S.G. were supported by national
funds (OE) through FCT, I.P., in the scope of the framework
contract foreseen in numbers 4, 5, and 6 of Article 23 of the
Decree-Law 57/2016 of August 29, changed by Law 57/2017
of July 19. Microphotographs were acquired in the LiM facility
of iBiMED/UA, a member of the Portuguese Platform of
BioImaging (PPBI) (POCI-01-0145-FEDER-022122).info:eu-repo/semantics/publishedVersio
Liprobe, a vital dye for lipid aggregates detection in imaging and high-content screens
Pathological lipid accumulation is a hallmark of several metabolic disorders, and
detection of lipid aggregates is an essential step for initial diagnosis and drug
screening purposes. However, low-cost, simple, and reliable detection
fluorescent probes are not widely available. Here, six push-pull-push dyes
were studied, and proved to be highly sensitive to the polarity of the
medium, presenting potential to distinguish structures with different
hydrophobic indexes. Importantly, in the presence of lipid aggregates their
staining specificity highly increased and the fluorescence wavelength blue
shifted. One of the compounds, named Liprobe, was physiologically inert in
cells, as witnessed by mass-spectrometry and metabolic assays. Liprobe was
not toxic to living zebrafish embryos, and differentially stained the muscle and
bone tissues. In triglyceride solutions, a high correlation was observed between
Liprobeâs 558 and 592 nm emissions and the 0â2.5 mg dlâ1 triglyceride range.
Confocal and cell-based high content screens revealed that this fluorophore
was able to selectively detect lipid droplets and ceramide loads in normal and
Farberâs disease human fibroblasts, respectively. Our results demonstrate that
Liprobe is a suitable fluorescing probe for vital staining of lipid aggregates,
compatible with a rapid and cheap high content screening assays for
preliminary diagnosis of Farberâs disease and, potentially, of other lipidosis.publishe
Multicomponent Synthesis of Luminescent Iminoboronates
A family of iminoboronates was prepared through a one-pot multicomponent reaction, starting from boronic acid, anthranilic acid, and different salicylaldehydes. Their synthesis was straightforward and the complexes were obtained in good to excellent yields. Their photophysical properties were assessed in a diluted solution, and the complexes proved to be faintly luminescent. These chelates demonstrated remarkable Aggregation-Induced Emission Enhancement, which was rationalized using crystal structures
SynthÚse de cyclodextrines multidifférenciées et mise en évidence de la chiralité de plateforme par la catalyse
Nous avons utilisé la méthode de déprotection sélective des éthers benzyliques au DIBAl-H pour synthétiser de nouvelles cyclodextrines possédant plusieurs groupements protecteurs différents sur leur couronne primaire, et notamment des motifs tétradifférenciés inaccessibles jusqu à présent. Ces molécules ont servi à la synthÚse d alpha- et béta-cyclodextrines amino-acides hydrosolubles, ainsi qu à l élaboration de chromophores contraints. Nous avons ensuite étudié la relation entre deux motifs régioisomÚres présentant des plateformes énantiomÚres l une de l autre. Pour cela, nous avons synthétisé des ligands diphosphine à partir de ces motifs régioisomÚres, et les avons engagés en catalyse asymétrique. Les résultats ont permis de valider le concept de plateforme chirale, et d élucider la relation de pseudo-énantiomérie entre les deux motifs. Enfin, nous avons synthétisé un ligand alpha-cyclodextrine-tétraphosphine qui a été engagé dans des tests catalytiques préliminaires.PARIS-BIUSJ-ThÚses (751052125) / SudocPARIS-BIUSJ-Physique recherche (751052113) / SudocSudocFranceF
Supramolecular organization of bis(3-halo-4-dimethylaminobenzylidene)hydrazines
Bis(3-bromo-4-dimethylaminobenzylidene)hydrazine and bis(3-chloro-4-dimethylaminobenzylidene)hydrazine have been synthesized, and their monoclinic crystal structures solved in space group P2(1)/c. For the bromo derivative at 150 K, a = 10.4505(2), b = 8.0358(2), c = 11.3208(2) angstrom, beta = 102.56(2)degrees, Z = 2, V = 927(9) angstrom(3), Dx = 1.618 g cm(-3), refinement on 2217 reflections, R = 0.023. For the chloro derivative at 150K, a = 10.3416(8), b = 8.0605(5), c = 11.1573(8) angstrom, beta = 102.70(8)degrees, Z = 2, V = 907(3) angstrom(3), Dx = 1.330 g cm(-3), refinement on 2429 reflections, R = 0.038. The molecules are close to planar, the crystal packing shows Br center dot center dot center dot Br or Cl center dot center dot center dot Cl intermolecular halogen bonds, and some C-H center dot center dot center dot Br or Cl contacts. There are no Cl center dot center dot center dot N or Br center dot center dot center dot N interactions. (c) 2012 Elsevier B.V. All rights reserved