Use of Alkanes to Estimate Dry Matter Intake of Beef Steers Grazing High Quality Pastures

Pastures remain the most important source of nutrients for ruminant livestock and nutrition is critical to optimize animal production. The daily quantity of dry matter that is consumed by an animal is a critical measurement to make nutritional inferences about feed and subsequent animal response. Researchers are facing the dilemma that, while estimates of individual animal performance are readily obtained, it is still difficult to estimate the herbage intake of individual animals. The objectives of this experiment were to estimate forage intake in beef steers grazing tall fescue (Festuca arundinacea Schreb.) and alfalfa (Medicago sativa)/tall fescue pastures and to measure the recovery rate of artificial alkanes from a controlled release device under these conditions

A Compact X-ray Source and Possible X-ray Jets within the Planetary Nebula Menzel 3

We report the discovery, by the Chandra X-ray Observatory, of X-ray emission from the bipolar planetary nebula Menzel 3. In Chandra CCD imaging, Mz 3 displays hot (3-6x10^6 K) gas within its twin, coaxial bubbles of optical nebulosity, as well as a compact X-ray source at the position of its central star(s). The brightest diffuse X-ray emission lies along the polar axis of the optical nebula, suggesting a jet-like configuration. The observed combination of an X-ray-emitting point source and possible X-ray jet(s) is consistent with models in which accretion disks and, potentially, magnetic fields shape bipolar planetary nebulae via the generation of fast, collimated outflows.Comment: 12 pages, 3 figures; to appear in Astrophysical Journal (Letters

Rhizosphere Microbiomes in a Historical Maize-Soybean Rotation System Respond to Host Species and Nitrogen Fertilization at the Genus and Subgenus Levels

Root-associated microbes are key players in plant health, disease resistance, and nitrogen (N) use efficiency. It remains largely unclear how the interplay of biological and environmental factors affects rhizobiome dynamics in agricultural systems. In this study, we quantified the composition of rhizosphere and bulk soil microbial communities associated with maize (Zea mays L.) and soybean (Glycine max L.) in a long-term crop rotation study under conventional fertilization and low-N regimes. Over two growing seasons, we evaluated the effects of environmental conditions and several treatment factors on the abundance of rhizosphere- and soil-colonizing microbial taxa. Time of sampling, host plant species, and N fertilization had major effects on microbiomes, while no effect of crop rotation was observed. Using variance partitioning as well as 16S sequence information, we further defined a set of 82 microbial genera and functional taxonomic groups at the subgenus level that show distinct responses to treatment factors. We identified taxa that are highly specific to either maize or soybean rhizospheres, as well as taxa that are sensitive to N fertilization in plant rhizospheres and bulk soil. This study provides insights to harness the full potential of soil microbes in maize and soybean agricultural systems through plant breeding and field management

The Chandra X-ray Survey of Planetary Nebulae (ChanPlaNS): Probing Binarity, Magnetic Fields, and Wind Collisions

We present an overview of the initial results from the Chandra Planetary Nebula Survey (ChanPlaNS), the first systematic (volume-limited) Chandra X-ray Observatory survey of planetary nebulae (PNe) in the solar neighborhood. The first phase of ChanPlaNS targeted 21 mostly high-excitation PNe within ~1.5 kpc of Earth, yielding 4 detections of diffuse X-ray emission and 9 detections of X-ray-luminous point sources at the central stars (CSPNe) of these objects. Combining these results with those obtained from Chandra archival data for all (14) other PNe within ~1.5 kpc that have been observed to date, we find an overall X-ray detection rate of ~70%. Roughly 50% of the PNe observed by Chandra harbor X-ray-luminous CSPNe, while soft, diffuse X-ray emission tracing shocks formed by energetic wind collisions is detected in ~30%; five objects display both diffuse and point-like emission components. The presence of X-ray sources appears correlated with PN density structure, in that molecule-poor, elliptical nebulae are more likely to display X-ray emission (either point-like or diffuse) than molecule-rich, bipolar or Ring-like nebulae. All but one of the X-ray point sources detected at CSPNe display X-ray spectra that are harder than expected from hot (~100 kK) central star photospheres, possibly indicating a high frequency of binary companions to CSPNe. Other potential explanations include self-shocking winds or PN mass fallback. Most PNe detected as diffuse X-ray sources are elliptical nebulae that display a nested shell/halo structure and bright ansae; the diffuse X-ray emission regions are confined within inner, sharp-rimmed shells. All sample PNe that display diffuse X-ray emission have inner shell dynamical ages <~5x10^3 yr, placing firm constraints on the timescale for strong shocks due to wind interactions in PNe.Comment: 41 pages, 6 figures; submitted to the Astronomical Journa

Potent Virucidal Activity In Vitro of Photodynamic Therapy with Hypericum Extract as Photosensitizer and White Light against Human Coronavirus HCoV-229E

The emergent human coronavirus SARS-CoV-2 and its high infectivity rate has highlighted the strong need for new virucidal treatments. In this sense, the use of photodynamic therapy (PDT) with white light, to take advantage of the sunlight, is a potent strategy for decreasing the virulence and pathogenicity of the virus. Here, we report the virucidal effect of PDT based on Hypericum extract (HE) in combination with white light, which exhibits an inhibitory activity of the human coronavirus HCoV-229E on hepatocarcinoma Huh-7 cells. Moreover, despite continuous exposure to white light, HE has long durability, being able to maintain the prevention of viral infection. Given its potent in vitro virucidal capacity, we propose HE in combination with white light as a promising candidate to fight against SARS-CoV-2 as a virucidal compound

Association analyses of host genetics, root-colonizing microbes, and plant phenotypes under different nitrogen conditions in maize

The root-associated microbiome (rhizobiome) affects plant health, stress tolerance, and nutrient use efficiency. However, it remains unclear to what extent the composition of the rhizobiome is governed by intraspecific variation in host plant genetics in the field and the degree to which host plant selection can reshape the composition of the rhizobiome. Here, we quantify the rhizosphere microbial communities associated with a replicated diversity panel of 230 maize (Zea mays L.) genotypes grown in agronomically relevant conditions under high N (+N) and low N (-N) treatments. We analyze the maize rhizobiome in terms of 150 abundant and consistently reproducible microbial groups and we show that the abundance of many root-associated microbes is explainable by natural genetic variation in the host plant, with a greater proportion of microbial variance attributable to plant genetic variation in -N conditions. Population genetic approaches identify signatures of purifying selection in the maize genome associated with the abundance of several groups of microbes in the maize rhizobiome. Genome-wide association study was conducted using the abundance of microbial groups as rhizobiome traits, and n=622 plant loci were identified that are linked to the abundance of n=104 microbial groups in the maize rhizosphere. In 62/104 cases, which is more than expected by chance, the abundance of these same microbial groups was correlated with variation in plant vigor indicators derived from high throughput phenotyping of the same field experiment. We provide comprehensive datasets about the three-way interaction of host genetics, microbe abundance, and plant performance under two N treatments to facilitate targeted experiments toward harnessing the full potential of root-associated microbial symbionts in maize production

An integrated approach to define new plays in mature oil basins: the example from the Middle Magdalena Valley basin (Colombia)

An integrated approach to detect new areas of potential interest associated with stratigraphic traps in mature basins is presented. The study was carried out in the Middle Magdalena Valley basin, Colombia. The workflow integrates outcrop and subsurface interpretationsoffacies,activityoffaults,anddistributionofdepocenters and paleocurrents and makes use of them to construct a threedimensionalexploration-scalegeocellularfaciesmodelofthebasin. The outcrop and well log sedimentological analysis distinguished faciesassociationsofalluvialfan,overbank, floodplain,andchannel fill,thelastoneconstitutingthereservoirrock.Theseismicanalysis showedthattectonicactivitywas coevalwiththedepositionofthe productive units in the basin and that the activity ended earlier (before the middle Miocene) along the western margin than along the eastern margin. Paleogeographic reconstructions depict transverse and longitudinal fluvial systems, alluvial fans adjacent to the activebasinmargins,and floodplainfaciesdominatingthestructural highs and the southwestern depositional limit. These reconstructions provided statistical data (lateral variograms) to construct the model. The exploration-scale facies model depicts the complete structureofthebasininthreedimensionsandthegrossdistribution of the reservoir and seal rocks. The predictive capability of the model was evaluated positively, and the model was employed to detect zones of high channel fill facies probability that form bodies that are isolated or that terminate upward in pinchouts or are truncated bya fault. Our approach canprovehelpfulinimproving general exploration workflows in similar settings

IFE Plant Technology Overview and contribution to HiPER proposal

HiPER is the European Project for Laser Fusion that has been able to join 26 institutions and signed under formal government agreement by 6 countries inside the ESFRI Program of the European Union (EU). The project is already extended by EU for two years more (until 2013) after its first preparatory phase from 2008. A large work has been developed in different areas to arrive to a design of repetitive operation of Laser Fusion Reactor, and decisions are envisioned in the next phase of Technology Development or Risk Reduction for Engineering or Power Plant facilities (or both). Chamber design has been very much completed for Engineering phase and starting of preliminary options for Reactor Power Plant have been established and review here

Mz 3, a Multipolar Nebula in the Making

The nebula Mz 3 has arguably the most complex bipolar morphology, consisting of three nested pairs of bipolar lobes and an equatorial ellipse. Its three pairs of bipolar lobes share the same axis of symmetry, but have very different opening angles and morphologies: the innermost pair of bipolar lobes shows closed lobe morphology, while the other two have open lobes with cylindrical and conical shapes, respectively. We have carried out high-dispersion spectroscopic observations of Mz 3, and detected distinct kinematic properties among the different morphological components. The expansion characteristics of the two outer pairs of lobes suggest that they originated in an explosive event, whereas the innermost pair of lobes resulted from the interaction of a fast wind with the surrounding material. The equatorial ellipse is associated with a fast equatorial outflow which is unique among bipolar nebulae. The dynamical ages of the different structures in Mz 3 suggest episodic bipolar ejections, and the distinct morphologies and kinematics among these different structures reveal fundamental changes in the system between these episodic ejections.Comment: To be published in the October issue of The Astronomical Journal. 16 pages, 10 figures. For full resolution figures, send requests to the Author [email protected]