Effect of Agricultural Structure Adjustment on Spatio-Temporal Patterns of Net Anthropogenic Nitrogen Inputs in the Pearl River Basin from 1990 to 2019

Worldwide urbanization has brought dramatic changes in agricultural structures, as well as serious agricultural non-point source pollutions of nitrogen and phosphorus. However, understanding the effect of agricultural structure adjustment on net anthropogenic nitrogen inputs (NANI) has been still limited. In this paper, statistical data from the agricultural statistical Yearbook, the National Economic and Social Development Statistical Bulletin were collected from 1990 to 2019 in the Pearl River Basin, China, and used to analyze the spatial and temporal patterns of NANI and its influencing factors. The results indicated that the agricultural structure adjustment has significantly influenced the spatial and temporal patterns of NANI in the last 30 years in the Pearl River Basin. The NANI decreased from 1990 to 2019, and had a spatial pattern of higher values in the upstream areas and lower in the downstream areas. In terms of the nitrogen input sources of NANI, in the economically developed regions downstream, nitrogen inputs are dominated by food/feed nitrogen, which accounted for an average of 49.6% of total nitrogen inputs. In upstream areas with relatively low economic development, fertilizer nitrogen accounted for an average of 54.9% of total nitrogen inputs. A novel nitrogen input source index of NANI, namely the ratio of agricultural nitrogen inputs to non-agricultural nitrogen inputs of NANI(ASNA), was also proposed to characterize the impact of the agricultural industry restructuring on NANI changes over time. Similar to the characteristics of NANI from 1990 to 2019, the ASNA showed a decreasing trend in the study area. Moreover, agricultural variables (agricultural land area, nitrogen fertilizer consumption and livestock farming density) tended to contribute less to the explained ASNA variances, while the contributions of the non-agricultural factors (population density and non-agricultural GDP) increased from 1990 to 2019. This indicated that the contribution of nitrogen inputs from agricultural sources to the NANI decreased while the contribution of nitrogen inputs from non-agricultural sources increased, with the shifts of agricultural sectors to the secondary and tertiary sectors in the Pearl River Basin. Our findings also suggest that differently regional targeting should be considered for the nitrogen pollution management in the Pearl River Basin, which focuses on the nitrogen pollution management of non-agricultural sources in the downstream areas, and but highlights agricultural nitrogen pollution management in the upstream areas

Two new quercetin glycoside derivatives from the fruits of <i>Gardenia jasminoides</i> var. <i>radicans</i>

<div><p>Two new quercetin glycoside derivatives named quercetin-3-<i>O</i>-[2-<i>O</i>-<i>trans</i>-caffeoyl-α-l-rhamnopyranosyl-(1 → 6)-β-d-glucopyranoside] (<b>1</b>) and quercetin-3-<i>O</i>-[2-<i>O</i>-<i>trans</i>-caffeoyl-β-l-rhamnopyranosyl-(1 → 6)-β-d-glucopyranoside] (<b>2</b>) along with three known flavonoids, 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone (<b>3</b>), 5,7-dihydroxy-8-methoxyflavone (<b>4</b>) and kaempferol 3-<i>O</i>-β-d-glucopyranoside (<b>5</b>), were isolated from the fruits of <i>Gardenia jasminoides</i> var. <i>radicans</i>. The structures of the new compounds were determined by means of extensive spectroscopic analysis (1D, 2D NMR and HR-ESI-MS), glycoside hydrolysis and sugar HPLC analysis after derivatisation. This is the first report on the isolation of a pair of compounds with α or β-l-rhamnopyranosyl configuration from plant and the first detail assignment of their NMR data.</p></div

EM23, A Natural Sesquiterpene Lactone from Elephantopus mollis, Induces Apoptosis in Human Myeloid Leukemia Cells through Thioredoxin- and Reactive Oxygen Species-Mediated Signaling Pathways

Elephantopus mollis H.B.K. (EM) is a traditional herbal medicine with multiple pharmacological activities. However, the efficacy of EM in treating human leukemia is currently unknown. In the current study, we report that EM23, a natural sesquiterpene lactone isolated from EM, inhibits the proliferation of human chronic myeloid leukemia K562 cells and acute myeloid leukemia HL-60 cells by inducing apoptosis. Translocation of membrane-associated phospholipid phosphatidylserines, changes in cell morphology, activation of caspases and cleavage of PARP were concomitant with this inhibition. The involvement of the mitochondrial pathway in EM23-mediated apoptosis was suggested by observed disruptions in mitochondrial membrane potential (MMP). Mechanistic studies indicated that EM23 caused a marked increase in the level of reactive oxygen species (ROS). Pretreatment with N-acetyl-L-cysteine (NAC), a ROS scavenger, almost fully reversed EM23-mediated apoptosis. In EM23-treated cells, the expression levels of thioredoxin (Trx) and thioredoxinreductase (TrxR), two components of the Trx system involved in maintaining cellular redox homeostasis, were significantly down-regulated. Concomitantly, Trx regulated the activation of apoptosis signal-regulating kinase 1 (ASK1) and its downstream regulatory targets, the p38, JNK, and ERK MAPKs. EM23-mediated activation of ASK1/MAPKs was significantly inhibited in the presence of NAC. Furthermore, tumor necrosis factor alpha (TNF-α)-mediated activation of nuclear factor-κB (NF-κB) was suppressed by EM23, as suggested by the observed blockage of p65 nuclear translocation, phosphorylation and reversion of IκBα degradation following EM23 treatment. Taken together, these results provide important insights into the anticancer activities of the EM component EM23 against human chronic myeloid leukemia K562 cells and acute myeloid leukemia HL-60 cells

Secondary metabolites from marine-derived <i>Streptomyces antibioticus</i> strain H74-21

<p>A new secondary metabolite, (2<i>S</i>,3<i>R</i>)-l-threonine, <i>N</i>-[3-(formylamino)-2-hydroxybenzoyl]-ethyl ester (streptomyceamide C, <b>1</b>), together with four known compounds 1, 4-dimethyl-3-isopropyl-2,5-piperidinedione (<b>2</b>), cyclo-((<i>S</i>)-Pro-8- hydroxy-(<i>R</i>)-Ile (<b>3</b>), cyclo-((<i>S</i>)-Pro-(<i>R</i>)-Leu (<b>4</b>), and seco-((<i>S</i>)-Pro-(<i>R</i>)-Val) (<b>5</b>), were isolated from the EtOH extract of the fermented mycelium of the marine-derived <i>streptomycete</i> strain H74-21, which was isolated from sea sediment in a mangrove site. The structure of the new compound was established on the basis of its spectroscopic data, including 1D and 2D NMR, HR-TOF-MS. Their antifungal activities against <i>Candida albicans</i> and cytotoxicities against human breast adenocarcinoma cell line MCF-7, human glioblastoma cell line SF-268 and human lung cancer cell line NCI-H460 were tested. Compounds <b>1</b> only displayed cytotoxicity against human breast adenocarcinoma cell line MCF-7 with the IC₅₀ value of 27.0 μg/mL. However, compounds <b>1</b>–<b>5</b> do not show antifungal activities at the test concentration of 1 mg/mL, and <b>2</b>–<b>5</b> have no cytotoxicities at the test concentration of 50 μg/mL.</p

Toward a systematic nomenclature for (neo)lignanamides

Phenylalkenoic acid amides, often referred to as phenol amides or hydroxycinnamic acid amides, are bioactive phytochemicals, whose bioactivity can be enhanced by coupling to form dimers or oligomers. Phenylalkenoic acid amides consist of a (hydroxy)cinnamic acid derivative (i.e., the phenylalkenoic acid subunit) linked to an amine-containing compound (i.e., the amine subunit) via an amide bond. The phenylalkenoic acid moiety can undergo oxidative coupling, either catalyzed by oxidative enzymes or due to autoxidation, which leads to the formation of (neo)lignanamides. Dimers described in the literature are often named after the species in which the compound was first discovered; however, the naming of these compounds lacks a systematic approach. We propose a new nomenclature, inspired by the existing system used for hydroxycinnamic acid dimers and lignin. In the proposed systematic nomenclature for (neo)lignanamides, compound names will be composed of three-letter codes and prefixes denoting the subunits, and numbers that indicate the carbon atoms involved in the linkage between the monomeric precursors. The proposed nomenclature is consistent, future-proof, and systematic

Trial of Endovascular Treatment of Acute Basilar-Artery Occlusion.

BACKGROUND: Data from trials investigating the effects and risks of endovascular thrombectomy for the treatment of stroke due to basilar-artery occlusion are limited. METHODS: We conducted a multicenter, prospective, randomized, controlled trial of endovascular thrombectomy for basilar-artery occlusion at 36 centers in China. Patients were assigned, in a 2:1 ratio, within 12 hours after the estimated time of basilar-artery occlusion to receive endovascular thrombectomy or best medical care (control). The primary outcome was good functional status, defined as a score of 0 to 3 on the modified Rankin scale (range, 0 [no symptoms] to 6 [death]), at 90 days. Secondary outcomes included a modified Rankin scale score of 0 to 2, distribution across the modified Rankin scale score categories, and quality of life. Safety outcomes included symptomatic intracranial hemorrhage at 24 to 72 hours, 90-day mortality, and procedural complications. RESULTS: Of the 507 patients who underwent screening, 340 were in the intention-to-treat population, with 226 assigned to the thrombectomy group and 114 to the control group. Intravenous thrombolysis was used in 31% of the patients in the thrombectomy group and in 34% of those in the control group. Good functional status at 90 days occurred in 104 patients (46%) in the thrombectomy group and in 26 (23%) in the control group (adjusted rate ratio, 2.06; 95% confidence interval [CI], 1.46 to 2.91, P CONCLUSIONS: In a trial involving Chinese patients with basilar-artery occlusion, approximately one third of whom received intravenous thrombolysis, endovascular thrombectomy within 12 hours after stroke onset led to better functional outcomes at 90 days than best medical care but was associated with procedural complications and intracerebral hemorrhage. (Funded by the Program for Innovative Research Team of the First Affiliated Hospital of USTC and others; ATTENTION ClinicalTrials.gov number, NCT04751708.)