Study on effect of active-fluxes in laser welding

The use of an active-flux in TIG welding has proven to increase the weld penetration and to improve productivity significantly. This paper describes experiments to apply active-fluxes, like SiO2, Cr2O3 and NaF mixtures in laser welding. The experiments were carried out using both CO2 and Nd:YAG lasers. During laser welding there is no influence of an electric current on the welding plume like in the TIG process. Nevertheless, some characteristics of the plume were affected by the components of the fluxes. This was detected by a spectroscopic analysis and is discussed in this paper. As a result of the fluxes, a deeper penetration has been achieved. Moreover, the typical nail head shape of the weld disappeared when using fluxes in Nd:YAG laser welding resulting in a constant width of the weld

Hydrological Cycle and Lake Water Source Indicated by Microrelief-Evaporite-Vegetation-Runoff Assemblage of Badain Jaran Desert

The hydrologic process of the sandy desert remains a focus in research in arid areas. Three major natural phenomena that can indicate the hydrological cycle in the extremely dry Badain Jaran Desert were found, namely the assemblage of megadune microrelief and evaporite, megadune vegetation and microrelief, as well as lakeside runoff and vegetation. The microrelief sand layer water, evaporite minerals, and lakeside hydrogeological features were analyzed by the drying and weighing method, environmental scanning electron microscopy with energy spectrum analysis functions, and a hydrogeological borehole survey. The water content of the microrelief 0-0.5 m sand layer is between 4.7% and 9.3%. The evaporite minerals are mainly composed of calcite (CaCO3) and gypsum (CaSO4). The shallow groundwater system in the off-shore area of lakes consists of an aeolian sand layer, a peat layer, and a lacustrine sedimentary layer, and the phreatic water with a thickness of 20 cm to 40 cm is reserved in the bottom of aeolian sand layer with a peat layer as a waterproof baseboard. Based on these results, the above three natural phenomena can be explained as follows: (1) The assemblage of megadune microrelief and evaporite was caused by the outcropping of water from megadune vadose zone in the form of preferential flow for a long time. Its leading edge differential wind erosion and calcium cemented fine sand layer indicate that water from the megadune vadose zone moves to and recharges the microrelief water along the micro-scale fine sand layer, during which, it features a multiple layer as it is controlled by a vertical dune bedding structure. (2) The small-scale assemblage of megadune vegetation and microrelief indicates that the water from the megadune vadose zone moved laterally and led to vegetation development, and the assemblage of microrelief and vegetation at a slope scale indicates that the vadose zone water presented multilayer enrichment and runoff producing due, to a great extent, to the bedding structures of different spacial locations. (3) The assemblage of lakeside runoff and vegetation is related to the phreatic water recharged by precipitation surrounding the lake, which indicates that the megadune water recharged by precipitation moved to the bottom of the megadune and constituted supply to the lake water. The three assemblages fully demonstrate that the megadune water recharged by precipitation in this desert could recharge the groundwater water and even lake water in the form of preferential flow due to the control of the bedding structure of different scales within the megadune. The results of lake water balance and the occurrence conditions of phreatic water surrounding the lake imply that the precipitation in this desert plays an important role in sustaining the lake. This study provides reliable evidence for revealing the essence of the hydrological cycle and the source of lake water in the Badain Jaran Desert, which indicates that although precipitation is small, it cannot be ignored in arid sandy desert areas

Neutrophil extracellular traps in cattle health and disease

Neutrophils largely contribute to the first line of defense against the invasion of pathogens. They kill pathogens basically by the following mechanisms: phagocytosis and proteolytic degradation, the release of enzymes with bactericidal activities, and the production of fibers to entrap pathogens, also known as neutrophil extracellular traps (NETs). NETs capture pathogens as a mechanism of immune protection and have been studied in-depth in various fields of human medicine. However, research about NETs in cattle is relatively scarce. The present article reviews the generation mechanisms, structural composition, signal pathways, advantages (and disadvantages) of NETs, and summarizes the latest findings of NETs in cattle health and disease

Neutrophil extracellular traps in cattle health and disease

Neutrophils largely contribute to the first line of defense against the invasion of pathogens. They kill pathogens basically by the following mechanisms: phagocytosis and proteolytic degradation, the release of enzymes with bactericidal activities, and the production of fibers to entrap pathogens, also known as neutrophil extracellular traps (NETs). NETs capture pathogens as a mechanism of immune protection and have been studied in-depth in various fields of human medicine. However, research about NETs in cattle is relatively scarce. The present article reviews the generation mechanisms, structural composition, signal pathways, advantages (and disadvantages) of NETs, and summarizes the latest findings of NETs in cattle health and disease

Facile route to bulk ultrafine-grain steels for high strength and ductility

Steels with sub-micrometre grain sizes usually possess high toughness and strength, which makes them promising for lightweighting technologies and energy-saving strategies. So far, the industrial fabrication of ultrafine-grained (UFG) alloys, which generally relies on the manipulation of diffusional phase transformation, has been limited to steels with austenite-to-ferrite transformation1,2,3. Moreover, the limited work hardening and uniform elongation of these UFG steels1,4,5 hinder their widespread application. Here we report the facile mass production of UFG structures in a typical Fe–22Mn–0.6C twinning-induced plasticity steel by minor Cu alloying and manipulation of the recrystallization process through the intragranular nanoprecipitation (within 30 seconds) of a coherent disordered Cu-rich phase. The rapid and copious nanoprecipitation not only prevents the growth of the freshly recrystallized sub-micrometre grains but also enhances the thermal stability of the obtained UFG structure through the Zener pinning mechanism6. Moreover, owing to their full coherency and disordered nature, the precipitates exhibit weak interactions with dislocations under loading. This approach enables the preparation of a fully recrystallized UFG structure with a grain size of 800 ± 400 nanometres without the introduction of detrimental lattice defects such as brittle particles and segregated boundaries. Compared with the steel to which no Cu was added, the yield strength of the UFG structure was doubled to around 710 megapascals, with a uniform ductility of 45 per cent and a tensile strength of around 2,000 megapascals. This grain-refinement concept should be extendable to other alloy systems, and the manufacturing processes can be readily applied to existing industrial production lines

Data_Sheet_1_Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistant Acinetobacter baumannii in bloodstream infections.PDF

ObjectiveAcinetobacter baumannii (A. baumannii, AB) represents a major species of Gram-negative bacteria involved in bloodstream infections (BSIs) and shows a high capability of developing antibiotic resistance. Especially, carbapenem-resistant Acinetobacter baumannii (CRAB) becomes more and more prevalent in BSIs. Hence, a rapid and sensitive CRAB detection method is of urgent need to reduce the morbidity and mortality due to CRAB-associated BSIs.MethodsA dual droplet digital PCR (ddPCR) reaction system was designed for detecting the antibiotic resistance gene OXA-23 and AB-specific gene gltA. Then, the specificity of the primers and probes, limit of detection (LOD), linear range, and accuracy of the assay were evaluated. Furthermore, the established assay approach was validated on 37 clinical isolates and compared with blood culture and drug sensitivity tests.ResultsThe dual ddPCR method established in this study demonstrated strong primer and probe specificity, distinguishing CRAB among 21 common clinical pathogens. The method showed excellent precision (3 × 10−4 ng/μL, CV 2 = 0.9976; gltA: y = 1.2716x + 3.6092, R2 = 0.9949). While the dual qPCR LOD is 3 × 10−3 ng/μL, the dual ddPCR’s LOD stands at 3 × 10−4 ng/μL, indicating a higher sensitivity in the latter. When applied to detect 35 patients with BSIs of AB, the results were consistent with clinical blood culture identification and drug sensitivity tests.ConclusionThe dual ddPCR detection method for OXA-23 and gltA developed in this study exhibits good specificity, excellent linearity, and a higher LOD than qPCR. It demonstrates reproducibility even for minute samples, making it suitable for rapid diagnosis and precision treatment of CRAB in BSIs.</p