507 research outputs found

    Bandwidth Estimation for IEEE 802.11-based Ad Hoc Networks

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    International audienceSince 2005, IEEE 802.11-based networks have been able to provide a certain level of quality of service (QoS) by the means of service differentiation, due to the IEEE 802.11e amendment. However, no mechanism or method has been standardized to accurately evaluate the amount of resources remaining on a given channel. Such an evaluation would, however, be a good asset for bandwidth-constrained applications. In multihop ad hoc networks, such evaluation becomes even more difficult. Consequently, despite the various contributions around this research topic, the estimation of the available bandwidth still represents one of the main issues in this field. In this paper, we propose an improved mechanism to estimate the available bandwidth in IEEE 802.11-based ad hoc networks. Through simulations, we compare the accuracy of the estimation we propose to the estimation performed by other state-of-the-art QoS protocols, BRuIT, AAC, and QoS-AODV

    ABE : Un protocole de réservation de bande passante pour les réseaux ad hoc basés sur IEEE 802.11

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    Poster à l'occasion du colloque CFIP 2006 : 4 pages.PosterLes recherches menées dans le domaine de la QoS pour les réseaux ad hoc ont connu un essor ces dernières années. La principale difficulté lors de la mise en place d'un protocole de QoS, réside dans l'estimation précise des ressources disponibles. Dans cet article, nous proposons une méthode fiable permettant d'estimer la bande passante résiduelle se basant sur une estimation probabiliste de la synchronisation des périodes de temps libre entre les mobiles émetteur et récepteur et une estimation de la probabilité de collision au niveau des liens radio. Par des simulations, nous comparons les performances de notre méthode avec d'autres protocoles de QoS basés sur d'autres techniques d'évaluation de la bande passante résiduelle

    Large Scale Terrain Generation from Tectonic Uplift and Fluvial Erosion

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    International audienceAt large scale, landscapes result from the combination of two major processes: tectonics which generate the main relief through crust uplift, and weather which accounts for erosion. This paper presents the first method in computer graphics that combines uplift and hydraulic erosion to generate visually plausible terrains. Given a user-painted uplift map, we generate a stream graph over the entire domain embedding elevation information and stream flow. Our approach relies on the stream power equation introduced in geology for hydraulic erosion. By combining crust uplift and stream power erosion we generate large realistic terrains at a low computational cost. Finally, we convert this graph into a digital elevation model by blending landform feature kernels whose parameters are derived from the information in the graph. Our method gives high-level control over the large scale dendritic structures of the resulting river networks, watersheds, and mountains ridges

    Whole-genome, deep pyrosequencing analysis of a duck influenza A virus evolution in swine cells.

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    We studied the sub-population level evolution of a duck influenza A virus isolate during passage in swine tracheal cells. The complete genomes of the A/mallard/Netherlands/10-Nmkt/1999 strain and its swine cell-passaged descendent were analysed by 454 pyrosequencing with coverage depth ranging from several hundred to several thousand reads at any point. This allowed characterization of defined minority sub-populations of gene segments 2, 3, 4, 5, 7, and 8 present in the original isolate. These minority sub-populations ranged between 9.5% (for segment 2) and 46% (for segment 4) of their respective gene segments in the parental stock. They were likely contributed by one or more viruses circulating within the same area, at the same period and in the same or a sympatric host species. The minority sub-populations of segments 3, 4, and 5 became extinct upon viral passage in swine cells, whereas the minority sub-populations of segments 2, 7 and 8 completely replaced their majority counterparts. The swine cell-passaged virus was therefore a three-segment reassortant and also harboured point mutations in segments 3 and 4. The passaged virus was more homogenous than the parental stock, with only 17 minority single nucleotide polymorphisms present above 5% frequency across the whole genome. Though limited here to one sample, this deep sequencing approach highlights the evolutionary versatility of influenza viruses whereby they exploit their genetic diversity, predilection for mixed infection and reassortment to adapt to a new host environmental niche.This work was supported by a grant from DEFRA and HEFCE under the Veterinary Training and Research Initiative to the Cambridge Infectious Diseases Consortium (VB, LT), BBSRC grants BB/H014306/1 and BB/G00479X/1 (LT), and the French Ministry of Agriculture, INRA and the French Région Midi-Pyrénées (GC, J-LG, VB).This is the accepted version of the original version available at: http://dx.doi.org/10.1016/j.meegid.2013.04.03

    Improving Accuracy in Available Bandwidth Estimation for 802.11-based Ad Hoc Networks

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    In this paper, we propose an accurate method to evaluate the available bandwidth in IEEE 802.11-based ad hoc networks. This method is based on a mechanism to predict the synchronisation of ilde periods and an estimation of collision probability on wireless link. We evaluate our solution on different scenarios. We also provide a comparison with different QoS routing protocols, BRuIT, AAC and QoS-AODV, based on different available bandwidth estimation techniques

    Clathrin-coated structures support 3D directed migration through local force transmission

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    Migrating cells navigate in complex environments through sensing and interpreting biochemical and/or mechanical cues. Here, we report that recently identified tubular clathrin/AP-2 lattices (TCALs), a subset of clathrin-coated structures (CCSs) that pinch collagen fibers, mechanically control directed migration along fibers decorated with ligands of CCS cargoes in three-dimensional (3D) environments. We observed that epidermal growth factor or low-density lipoprotein bound to collagen fibers leads to increased local nucleation and accumulation of TCALs. By using engineered, mixed collagen networks, we demonstrate that this mechanism selectively increases local forces applied on ligand-decorated fibers. We show that these effects depend on the ligand’s receptors but do not rely on their ability to trigger signaling events. We propose that the preferential accumulation of TCALs along ligand-decorated fibers steers migration in 3D environments. We conclude that ligand-regulated, local TCAL accumulation results in asymmetric force distribution that orients cell migration in 3D environments

    An amplicon-based nanopore sequencing workflow for rapid tracking of avian influenza outbreaks, France, 2020-2022

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    During the recent avian influenza epizootics that occurred in France in 2020/21 and 2021/22, the virus was so contagiousness that it was impossible to control its spread between farms. The preventive slaughter of millions of birds consequently was the only solution available. In an effort to better understand the spread of avian influenza viruses (AIVs) in a rapid and innovative manner, we established an amplicon-based MinION sequencing workflow for the rapid genetic typing of circulating AIV strains. An amplicon-based MinION sequencing workflow based on a set of PCR primers targeting primarily the hemagglutinin gene but also the entire influenza virus genome was developed. Thirty field samples from H5 HPAIV outbreaks in France, including environmental samples, were sequenced using the MinION MK1C. A real-time alignment of the sequences with MinKNOW software allowed the sequencing run to be stopped as soon as enough data were generated. The consensus sequences were then generated and a phylogenetic analysis was conducted to establish links between the outbreaks. The whole sequence of the hemagglutinin gene was obtained for the 30 clinical samples of H5Nx HPAIV belonging to clade 2.3.4.4b. The consensus sequences comparison and the phylogenetic analysis demonstrated links between some outbreaks. While several studies have shown the advantages of MinION for avian influenza virus sequencing, this workflow has been applied exclusively to clinical field samples, without any amplification step on cell cultures or embryonated eggs. As this type of testing pipeline requires only a short amount of time to link outbreaks or demonstrate a new introduction, it could be applied to the real-time management of viral epizootics

    Towards an improvement of optically stimulated luminescence (OSL) age uncertainties:Modelling OSL ages with systematic errors, stratigraphic constraints and radiocarbon ages using the R package BayLum

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    International audienceAbstract. Statistical analysis has become increasingly important in optically stimulated luminescence (OSL) dating since it has become possible to measure signals at the single-grain scale. The accuracy of large chronological datasets can benefit from the inclusion, in chronological modelling, of stratigraphic constraints and shared systematic errors. Recently, a number of Bayesian models have been developed for OSL age calculation; the R package “BayLum” presented herein allows different models of this type to be implemented, particularly for samples in stratigraphic order which share systematic errors. We first show how to introduce stratigraphic constraints in BayLum; then, we focus on the construction, based on measurement uncertainties, of dose covariance matrices to account for systematic errors specific to OSL dating. The nature (systematic versus random) of errors affecting OSL ages is discussed, based – as an example – on the dose rate determination procedure at the IRAMAT-CRP2A laboratory (Bordeaux). The effects of the stratigraphic constraints and dose covariance matrices are illustrated on example datasets. In particular, the benefit of combining the modelling of systematic errors with independent ages, unaffected by these errors, is demonstrated. Finally, we discuss other common ways of estimating dose rates and how they may be taken into account in the covariance matrix by other potential users and laboratories. Test datasets are provided as a Supplement to the reader, together with an R markdown tutorial allowing the reproduction of all calculations and figures presented in this study
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