Impedance analysis of endothelial cells undergoing orbital shear stress.

Understanding the endothelium at the cellular level could further knowledge of the cardiovascular system as a whole and could therefore lead to advances in the prevention and treatment of cardiovascular disease. Electric cell-substrate impedance sensing is an in vitro technique that can be used for observing the behavior of endothelial cells in real-time using a fluid flow environment to simulate the circulatory system. This study examined the effect of fluid shear stress on human umbilical vein endothelial cells using electrochemical impedance spectroscopy (impedance sensing). Circuit models were fit to empirical data to measure cell layer resistance and capacitance changes, and to determine if data trends follow those of previously published findings. Information derived from transendothelial electrical resistance measurements, about changes in cell layer permeability when subjected to varying shear stress conditions within an orbiting circular well, was used to draw conclusions aided by microscopic images of the cells

Dynamic parameters of structures extracted from ambient vibration measurements: an aid for the seismic vulnerability assessment of existing buildings in moderate seismic hazard regions

During the past two decades, the use of ambient vibrations for modal analysis of structures has increased as compared to the traditional techniques (forced vibrations). The Frequency Domain Decomposition method is nowadays widely used in modal analysis because of its accuracy and simplicity. In this paper, we first present the physical meaning of the FDD method to estimate the modal parameters. We discuss then the process used for the evaluation of the building stiffness deduced from the modal shapes. The models considered here are 1D lumped-mass beams and especially the shear beam. The analytical solution of the equations of motion makes it possible to simulate the motion due to a weak to moderate earthquake and then the inter-storey drift knowing only the modal parameters (modal model). This process is finally applied to a 9-storey reinforced concrete (RC) dwelling in Grenoble (France). We successfully compared the building motion for an artificial ground motion deduced from the model estimated using ambient vibrations and recorded in the building. The stiffness of each storey and the inter-storey drift were also calculated

Noninflammatory Changes of Microglia Are Sufficient to Cause Epilepsy.

Microglia are well known to play a critical role in maintaining brain homeostasis. However, their role in epileptogenesis has yet to be determined. Here, we demonstrate that elevated mTOR signaling in mouse microglia leads to phenotypic changes, including an amoeboid-like morphology, increased proliferation, and robust phagocytosis activity, but without a significant induction of pro-inflammatory cytokines. We further provide evidence that these noninflammatory changes in microglia disrupt homeostasis of the CNS, leading to reduced synapse density, marked microglial infiltration into hippocampal pyramidal layers, moderate neuronal degeneration, and massive proliferation of astrocytes. Moreover, the mice thus affected develop severe early-onset spontaneous recurrent seizures (SRSs). Therefore, we have revealed an epileptogenic mechanism that is independent of the microglial inflammatory response. Our data suggest that microglia could be an opportune target for epilepsy prevention

Connectivity clues from short-term variability in settlement and geochemical tags of mytilid mussels

The use of geochemical tags in calcified structures of fish and invertebrates is an exciting tool for investigating larval population connectivity. Tag evaluation over relatively short intervals (weeks) may detect environmental and ecological variability at a temporal scale highly relevant to larval transport and settlement. We collected newly settled mussels (Mytilus californianus and M. galloprovincialis) weekly during winter/spring of 2002 along the coast of San Diego, CA, USA, at sites on the exposed coast (SIO) and in a protected coastal bay (HI), to investigate temporal patterns of geochemical tags in mussel shells. Analyses of post-settlement shell via LA-ICP-MS revealed statistically significant temporal variability for all elements we examined (Mg, Mn, Cu, Sr, Cd, Ba, Pb and U). Despite this, our ability to distinguish multielemental signatures between sites was largely conserved. Throughout our 13-week study, SIO and HI mussels could be chemically distinguished from one another in 78-87% of all cases. Settlement varied between 2 and 27 settlers gram-byssus-1week-1 at SIO and HI, and both sites were characterized by 2-3weeks with "high" settlement. Geochemical tags recorded in early larval shell of newly settled mussels differed between "high" and "low" settlement weeks at both sites (MANOVA), driven by Mg and Sr at SIO (p=0.013) and Sr, Cd, Ba and Pb at HI (p<0.001). These data imply that shifts in larval sources or transport corridors were responsible for observed settlement variation, rather than increased larval production. In particular, increased settlement at HI was observed concurrent with the appearance of geochemical tags (e.g., elevated Cd), suggesting that those larvae were retained in upwelled water near the mouth of the bay. Such shifts may reflect short-term changes in connectivity among sites due to altered transport corridors, and influence the demography of local populations

Geokinematics of Central Europe: New insights from the CERGOP-2/Environment Project

The Central European Geodynamics Project CERGOP/2, funded by the European Union from 2003to 2006 under the 5th Framework Programme, benefited from repeated measurements of thecoordinates of epoch and permanent GPS stations of the Central European GPS Reference Network(CEGRN), starting in 1994. Here we report on the results of the systematic processing of availabledata up to 2005. The analysis has yielded velocities for some 60 sites, covering a variety of CentralEuropean tectonic provinces, from the Adria indenter to the Tauern window, the Dinarides, thePannonian Basin, the Vrancea seismic zone and the Carpathian Mountains. The estimated velocitiesdefine kinematical patterns which outline, with varying spatial resolution depending on the stationdensity and history, the present day surface kinematics in Central Europe. Horizontal velocities areanalyzed after removal from the ITRF2000 estimated velocities of a rigid rotation accounting forthe mean motion of Europe: a ~2.3 mm/yr north-south oriented convergence rate between Adria andthe Southern Alps that can be considered to be the present day velocity of the Adria indenterrelative to the European foreland. An eastward extrusion zone initiates at the Tauern Window. Thelateral eastward flow towards the Pannonian Basin exhibits a gentle gradient from 1-1.5 mm/yrimmediately east of the Tauern Window to zero in the Pannonian Basin. This kinematic continuityimplies that the Pannonian plate fragment recently suggested by seismic data does not require aspecific Eulerian pole. On the southeastern boundary of the Adria microplate, we report a velocitydrop from 4-4.5 mm/yr motion near Matera to ~1 mm/yr north of the Dinarides, in the southwesternpart of the Pannonian Basin. A positive velocity gradient as one moves south from West Ukraineacross Rumania and Bulgaria is estimated to be 2 mm/yr on a scale of 600-800 km, as if the crustwere dragged by the counterclockwise rotation along the North Anatolian Fault Zone. This regimeapparently does not interfere with the Vrancea seismic zone: earthquakes there are sufficiently deep(&gt; 100 km) that the brittle deformation at depth can be considered as decoupled from the creep atthe surface. We conclude that models of the Quaternary tectonics of Central and Eastern Europeshould not neglect the long wavelength, nearly aseismic deformation affecting the upper crust in theRomanian and Bulgarian regions

Invertebrate population genetics across Earth's largest habitat: The deep-sea floor

Despite the deep sea being the largest habitat on Earth, there are just 77 population genetic studies of invertebrates (115 species) inhabiting non-chemosynthetic ecosystems on the deep-sea floor (below 200 m depth). We review and synthesize the results of these papers. Studies reveal levels of genetic diversity comparable to shallow-water species. Generally, populations at similar depths were well connected over 100s–1,000s km, but studies that sampled across depth ranges reveal population structure at much smaller scales (100s–1,000s m) consistent with isolation by adaptation across environmental gradients, or the existence of physical barriers to connectivity with depth. Few studies were ocean-wide (under 4%), and 48% were Atlantic-focused. There is strong emphasis on megafauna and commercial species with research into meiofauna, “ecosystem engineers” and other ecologically important species lacking. Only nine papers account for ~50% of the planet's surface (depths below 3,500 m). Just two species were studied below 5,000 m, a quarter of Earth's seafloor. Most studies used single-locus mitochondrial genes revealing a common pattern of non-neutrality, consistent with demographic instability or selective sweeps; similar to deep-sea hydrothermal vent fauna. The absence of a clear difference between vent and non-vent could signify that demographic instability is common in the deep sea, or that selective sweeps render single-locus mitochondrial studies demographically uninformative. The number of population genetics studies to date is miniscule in relation to the size of the deep sea. The paucity of studies constrains meta-analyses where broad inferences about deep-sea ecology could be made

Ein Schtetl in der Stadt – Jüdische Identitätsräume in Texten von Martin Beradt und Sammy Gronemann

The concern of this thesis is a discussion of the way German-Jewish identity manifests itself in two literary texts before and after 1933. Using the examples of Sammy Gronemann’s novel Tohuwabohu and Martin Beradt’s Die Straße der kleinen Ewigkeit, it offers a textual analysis of two works which share close connections in terms of subject matter, style, and their respective authors’ background, but are historically divided by the fundamental experience of the rise of National Socialism in Germany. I argue that space is a crucial factor through which identity is constituted in each text, both of which use and partially subvert the romanticized image of the Eastern European shtetl brought to Germany by authors such as Arnold Zweig in the aftermath of World War I. Space in this context always has a twofold quality to it. It functions as a space of identity, but also as a space of identification through which a group of people label others as either belonging or not belonging to a specific space. Furthermore, both texts reject monolithic definitions of Jewish identity, emphasizing instead the diversity of Jewish life in Europe before the Rise of National Socialism

Ein Schtetl in der Stadt – Jüdische Identitätsräume in Texten von Martin Beradt und Sammy Gronemann

The concern of this thesis is a discussion of the way German-Jewish identity manifests itself in two literary texts before and after 1933. Using the examples of Sammy Gronemann’s novel Tohuwabohu and Martin Beradt’s Die Straße der kleinen Ewigkeit, it offers a textual analysis of two works which share close connections in terms of subject matter, style, and their respective authors’ background, but are historically divided by the fundamental experience of the rise of National Socialism in Germany. I argue that space is a crucial factor through which identity is constituted in each text, both of which use and partially subvert the romanticized image of the Eastern European shtetl brought to Germany by authors such as Arnold Zweig in the aftermath of World War I. Space in this context always has a twofold quality to it. It functions as a space of identity, but also as a space of identification through which a group of people label others as either belonging or not belonging to a specific space. Furthermore, both texts reject monolithic definitions of Jewish identity, emphasizing instead the diversity of Jewish life in Europe before the Rise of National Socialism

Conservation genetics of California abalone species

Over the past three decades, five species of abalone (genus Haliotis) in California waters have gone from harvestable resources to rare or even endangered species status. Both anthropogenic (overexploitation, habitat destruction) and natural (disease, predation) factors have contributed to the declines. This dissertation explores several aspects of abalone recovery and conservation along the coast of California through population genetic theory and techniques. Chapters I and II deal with the captive breeding of two California abalone species. Chapter I focuses on ensuring the specific purity of broodstock and maintaining genetic diversity in hatchery-bred progeny of the endangered white abalone (H. soreseni). Using DNA from these animals, genetic markers were developed, including five nuclear microsatellite loci and partial sequences of one nuclear (VERL) and two mitochondrial (COI and CytB) genes, to assess genetic variability in the species, aid in species identification (ergo prevent broodstock contamination), and potentially track the success of future outplanting of captive-reared animals in restocking operations. Chapter II quantifies the loss of genetic diversity resulting from the captive breeding of green abalone (H. fulgens). No change in overall heterozygosity was evident, but significant losses in allelic richness were found in the captive-bred green abalone versus that in wild populations. Chapters III, IV, and V center on inferring realized connectivity (gene flow) among natural populations of red (H. rufescens), black (H. cracherodii), and pink (H. corrugata) abalone. For red abalone (Chapter III), COI sequencing and microsatellite genotyping did not show significant genetic divergence among populations. In contrast, data from AFLPs became the first to suggest there is significant genetic differentiation among California red abalone populations. In black abalone (Chapter IV), data from AFLPs and one microsatellite locus showed significant divergence among multiple populations and exhibited a signal of isolation by distance consistent with a stepping-stone model of connectivity. Pink abalone (Chapter V) also showed evidence of restricted gene flow among populations. Finally, Chapters VI and VII focus on the fatal disease withering syndrome (WS). Chapter VI involves characterizing the post-esophageal microbiomes of red and black abalone with and without WS. Significant differences between the clone libraries isolated from healthy and diseased abalone suggested that WS has significant impact on the bacterial composition of the abalone post-esophageal microbiome, and specific membership of the microbiomes suggests that infection by Candidatus Xenohaliotis californiensis, the etiological agent of WS, may not be the sole cause of morbidity and mortality due to WS in abalone. Chapter VII is a preliminary study of genetic variation within C. X. californiensis samples from wild and captive-bred abalone species collected from multiple locations along the coast of California. Thus far, no variation has been found in partial sequences of either the 16S rDNA or recombinase A (recA) genes