A Systems Biology Approach to Characterize the Regulatory Networks Leading to Trabectedin Resistance in an In Vitro Model of Myxoid Liposarcoma

Trabectedin, a new antitumor compound originally derived from a marine tunicate, is clinically effective in soft tissue sarcoma. The drug has shown a high selectivity for myxoid liposarcoma, characterized by the translocation t(12;16)(q13; p11) leading to the expression of FUS-CHOP fusion gene. Trabectedin appears to act interfering with mechanisms of transcription regulation. In particular, the transactivating activity of FUS-CHOP was found to be impaired by trabectedin treatment. Even after prolonged response resistance occurs and thus it is important to elucidate the mechanisms of resistance to trabectedin. To this end we developed and characterized a myxoid liposarcoma cell line resistant to trabectedin (402-91/ET), obtained by exposing the parental 402-91 cell line to stepwise increases in drug concentration. The aim of this study was to compare mRNAs, miRNAs and proteins profiles of 402-91 and 402-91/ET cells through a systems biology approach. We identified 3,083 genes, 47 miRNAs and 336 proteins differentially expressed between 402-91 and 402-91/ET cell lines. Interestingly three miRNAs among those differentially expressed, miR-130a, miR-21 and miR-7, harbored CHOP binding sites in their promoter region. We used computational approaches to integrate the three regulatory layers and to generate a molecular map describing the altered circuits in sensitive and resistant cell lines. By combining transcriptomic and proteomic data, we reconstructed two different networks, i.e. apoptosis and cell cycle regulation, that could play a key role in modulating trabectedin resistance. This approach highlights the central role of genes such as CCDN1, RB1, E2F4, TNF, CDKN1C and ABL1 in both pre- and post-transcriptional regulatory network. The validation of these results in in vivo models might be clinically relevant to stratify myxoid liposarcoma patients with different sensitivity to trabectedin treatment

DH as an Ideal Educational Environment: the Ethnographic Museum of La Spezia

The authors present the outcomes of an educational experimentation that took place in the academic year 2018-2019 at the degree course in Informatica Umanistica at the University of Pisa. The first objective of the project concerned the digitization of a corpus of postcards from the period of the First World War owned by the ethnographic Museum of La Spezia “G. Podenzana”. The aims of the work are not only the historical study of the corpus, but also the organization of a public history project with the Museum.Gli autori presentano i risultati di una sperimentazione didattica svolta durante l’anno accademico 2018-2019 presso il Corso di Laurea in Informatica Umanistica dell’Università di Pisa. Il primo obiettivo del progetto riguarda la digitalizzazione di un corpus di cartoline del periodo della Prima Guerra Mondiale, di proprietà del Museo Etnografico di La Spezia “G. Podenzana”. Gli obiettivi del lavoro non sono solo lo studio storico del corpus, ma anche l’organizzazione di un progetto di Public History con il Museo

AIUCD 2021 - DH per la società: e-guaglianza, partecipazione, diritti e valori nell’era digitale - DHs for society: e-quality, participation, rights and values in the Digital Age. Book of extended abstracts of the 10th national conference

All the extended abstracts published in this volume have received favourable reviews by experts in the field of DH, through an anonymous double-blind peer review process under the responsibility of the AIUCD 2021 Scientific Committee. The AIUCD 2021 conference program is available online https://aiucd2021.labcd.unipi.it/Gli abstract estesi pubblicati in questo volume hanno ottenuto il parere favorevole da parte di valutatori esperti della materia, attraverso un processo di revisione anonima mediante double-blind peer review sotto la responsabilità del Comitato Scientifico di AIUCD 2021. Il programma della conferenza AIUCD è disponibile online https://aiucd2021.labcd.unipi.it

From collaborative transcription to interdisciplinary education: the postcards of the Great War case

The authors intend to present an educational experimentation that took place in the first semester of the academic year 2018-2019 at the degree course in Informatica Umanistica at the University of Pisa. The experimentation involved the courses of Digital Public History, Digital Text Encoding as well as Digital Philology, and concerned the digitization of a corpus of postcards from the period of the First World War. The postcards, owned by the ethnographic museum of La Spezia “G. Podenzana” and in collaboration with it, have been historically contextualized, digitized, placed on a platform and distributed to the students in order to be recorded, transcribed and encoded with the following purposes:  make students participate in a real Digital Public History project;  consider the problems of how to record, transcribe, share and encode a corpus of historical postcards;  evaluate how to combine more educational activities into a wider project of crowdsourcing;  test a collaborative platform and some encoding tools;  test methods and timing to allow the collaboration between individual courses within a university degree course in DH. The authors aim to highlight the main problems encountered and the added values of the integration of courses in teaching Digital Humanities

A un anno da AIUCD2021

Introduction to the AIUCD2021 conference proceedings.Introduzione agli atti del convegno AIUCD2021

Prefazione

prefazione agli atti del convegn