Thiotriazoline ointment effects modification by incorporating silver nanoparticles in experimental thermal burns

Grin I. V., Zvyagintsevа T. V., Naumova O. V., Grin V. V. Thiotriazoline ointment effects modification by incorporating silver nanoparticles in experimental thermal burns. Journal of Education, Health and Sport. 2016;6(12):916-928. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.268951http://ojs.ukw.edu.pl/index.php/johs/article/view/4241    The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 754 (09.12.2016).754 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 05.12.2016. Revised 20.12.2016. Accepted: 25.12.2016. UDK: 615.27:615.31:546.57-022.532:616-001.17-092.9 Thiotriazoline ointment effects modification by incorporating silver nanoparticles in experimental thermal burns I. V. Grin, T. V. Zvyagintsevа, O. V. Naumova, V. V. Grin  Kharkiv National Medical University, Kharkov, [email protected] AbstractWe evaluated Thiotriazoline ointment effects modification by incorporating silver nanoparticles in experimental thermal burns. Studies were carried out on 78 rat population WAG (4 groups, each group of 6 rats): 1 – intact; 2 – burn (without treatment), 3 – burn + "Thiotriazoline ointment 2%", the reference drug; 4 – burn + Thiotriazoline ointment with silver nanoparticles (0.00081%). We studied the skin morphological changes upon thermal damage. We have found that the incorporation of silver nanoparticles in Thiotriazoline ointment modifies its healing action; reparative process takes place more intensively than with Thiotriazoline ointments, which is confirmed by destructive process restriction within epidermis and derma, epithelialization acceleration and complete closure of the defect at 21th day of thermal burn healing versus 28th day with reference drug use.Keywords: Thiotriazoline ointment with silver nanoparticles, thermal burns

GCView: the genomic context viewer for protein homology searches

Genomic neighborhood can provide important insights into evolution and function of a protein or gene. When looking at operons, changes in operon structure and composition can only be revealed by looking at the operon as a whole. To facilitate the analysis of the genomic context of a query in multiple organisms we have developed Genomic Context Viewer (GCView). GCView accepts results from one or multiple protein homology searches such as BLASTp as input. For each hit, the neighboring protein-coding genes are extracted, the regions of homology are labeled for each input and the results are presented as a clear, interactive graphical output. It is also possible to add more searches to iteratively refine the output. GCView groups outputs by the hits for different proteins. This allows for easy comparison of different operon compositions and structures. The tool is embedded in the framework of the Bioinformatics Toolkit of the Max-Planck Institute for Developmental Biology (MPI Toolkit). Job results from the homology search tools inside the MPI Toolkit can be forwarded to GCView and results can be subsequently analyzed by sequence analysis tools. Results are stored online, allowing for later reinspection. GCView is freely available at http://toolkit.tuebingen.mpg.de/gcview

Inertial parameters and superfluid-to-normal phase transition in superdeformed bands

The quasiclassically exact solution for the second inertial parameter $\cal B$ is found in self-consistent way. It is shown that superdeformation and nonuniform pairing arising from the rotation induced pair density significantly reduce this inertial parameter. The different limiting cases for $\cal B$, which allow to study an interplay between rapid rotation, pairing correlations, and mean field deformation, are considered. The new signature for the transition from pairing to normal phase is suggested in terms of the variation of ${\cal B}/{\cal A}$ versus spin. Experimental data indicate the existence of such transition in the three superdeformed mass regions.Comment: 8 pages, LaTeX, 3 figure

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane

Autotransport in Gram-negative bacteria denotes the ability of surface-localized proteins to cross the outer membrane (OM) autonomously. Autotransporters perform this task with the help of a β-barrel transmembrane domain localized in the OM. Different classes of autotransporters have been investigated in detail in recent years; classical monomeric but also trimeric autotransporters comprise many important bacterial virulence factors. So do the two-partner secretion systems, which are a special case as the transported protein resides on a different polypeptide chain than the transporter. Despite the great interest in these proteins, the exact mechanism of the transport process remains elusive. Moreover, different periplasmic and OM factors have been identified that play a role in the translocation, making the term ‘autotransport’ debatable. In this review, we compile the wealth of details known on the mechanism of single autotransporters from different classes and organisms, and put them into a bigger perspective. We also discuss recently discovered or rediscovered classes of autotransporters

БИОТЕХНОЛОГИЧЕСКИЕ ПРОЦЕССЫ ПРИ ПЕРЕРАБОТКЕ ОТХОДОВ

Modern biotechnology is a direction designed to find ways of industrial application of biological agents and processes. This is a complex multidisciplinary field, including microbiological synthesis, genetic, protein and cell engineering, engineering enzymology. Biotechnology is mainly based on the use of microorganisms. Therefore, the knowledge accumulated by microbiology about the diversity of the world, about the structure, genetics, physiology, variability, ecology of microbes creates a scientific basis for the development of many biotechnological industries. Traditional raw materials for various branches of the chemical and processing industries (oil and gas) are depleted, and this will lead to the fact that biomass resources will be increasingly used. In addition to new methods for producing chemicals from biomass, bio-technology also gives us more efficient and industrial catalysts for chemical interconversions. A promising area for further development is the production of valuable substances from plants, such as terpenes and alkaloids used in the manufacture of drugs; currently 25 % of all medicines are made from plants. Using animal cells, the production of viruses for vaccines is possible. In the long term, these are hepatitis A, hepatitis B, herpes type 1 viruses, herpes type 2 viruses, as well as viruses that cause colds, some forms of cancer and dental caries. Indexing:             Современная биотехнология – это направление, призванное изыскивать пути промышленного применения биологических агентов и процессов. Это комплексная многопрофильная область, включающая микробиологический синтез, генетическую, белковую и клеточную инженерию, инженерную энзимологию.  Биотехнология в основном опирается на использование микроорганизмов. Поэтому знания, накопленные микробиологией о многообразии мира, о строении, генетике, физиологии, изменчивости, экологии микробов создают научную основу для развития многих биотехнологических производств. Традиционное сырье для различных отраслей химической и перерабатывающей промышленности (нефть и газ) истощается, а это приведет к тому, что всё более широко будут использоваться ресурсы биомассы.  Помимо новых способов получения химических веществ из биомассы, биотехнология дает нам также более эффективные и производственные катализаторы для осуществления химических взаимопревращений.  Многообещающей областью дальнейшего развития представляется производство ценных веществ из растений, например терпенов и алкалоидов, используемых при производстве лекарств; в настоящее время 25 % всех лекарств производится из растений. С помощью животных клеток возможна продукция вирусов для получения вакцин. В перспективе это вирусы гепатита А, гепатита Б, герпеса типа 1, герпеса типа 2, а также вирусы, вызывающие простудные заболевания, некоторые формы рака и зубной кариес.  Індексація:               &nbsp

Swim test for joint angles dispersion analysis during hind limb motor function assessment in SCI models

In the most of functional studies, various stress tests are used to assess functional improvement following spinal cord injury in animal models. However, available methods of motor function evaluation are not always accurate and unbiased. The main objective of the study was to create a new method of motor activity assessment in minor animal models of spinal cord injury. This method should provide an objective and accurate evaluation of limb motor function in models having severe neurological disorders following contusion spinal cord injury. The swimming test was used as the key behavioral test. To assess the motor function of swimming animals’ hind limbs, we measured angles of hind limbs movements adjusted to the motion direction axis. Then we calculated individual angles dispersion for each joint and limb using the parameters of angles sample dispersion and amplitude-depending dispersion. The current study included two groups of Sprague-Dawley rats: control group and a group of animals having moderate thoracic spinal cord contusion injury. Control animals demonstrated stable dispersion indicators for 6 weeks of follow-up. In the experimental group, a tendency to the improvement of motor function in hind limbs between 1 and 3 weeks was revealed followed by stabilization and preservation of both indicators between 3 and 6 weeks. Provided method based on the measurement of joint angles adjusted to the movement direction axis followed by calculation of indicators of variance of a random variable and amplitude-depending variance can be an effective and objective alternative for motor function evaluation

Superfluid to normal phase transition and extreme regularity of superdeformed bands

We derive the exact semiclassical expression for the second inertial parameter $\cal B$ for the superfluid and normal phases. Interpolation between these limiting values shows that the function ${\cal B}(I)$ changes sign at the spin $I_c$, which is critical for a rotational spectrum. The quantity $\cal B$ turns out to be a sensitive measure of the change in static pairing correlations. The superfluid-to-normal transition reveals itself in the specific variation of the ratio ${\cal B}/{\cal A}$ versus spin $I$ with the plateau characteristic of the normal phase. We find this dependence to be universal for normal deformed and superdeformed bands. The long plateau with a small value ${\cal B}/{\cal A}\sim A^{-8/3}$ explains the extreme regularity of superdeformed bands.Comment: 30 pages in LaTeX, 6 figures (PostScript). To be published in Yadernaya Fizika (Physics of Atomic Nuclei), special edition dedecated to the 90th birthday of Prof. I. I. Gurevit

The choice of surgical approach in the treatment of two-column acetabular fractures

The study aimed a comparison of treatment results in patients with two-column acetabular fractures (AO, Type C) when using different operative approaches: ilioinguinal (12), Y-type (16) and a combination of posterior-lateral and ilioinguinal approaches (3). Surgical treatment was carried out not later than 3 weeks after injury. The joint congruence was reached in all the cases. The operative time, blood loss, complications, long-term results of treatment were evaluated. The study showed the effectiveness of ilioinguinal approach in case of the simple two-column fractures. When treating patients with complex fractures of the anterior column, the authors found no significant difference in operative time and intraoperative blood loss between the cases of V-type and combined approaches. In patients with two-column fractures accompanied with posterior wall lesion the time of surgery was significantly lower when using the Y-shaped approach as against the two different approaches

Interobserver Agreement for Endometrial Cancer Characteristics Evaluated on Biopsy Material

A shift toward a disease-based therapy designed according to patterns of failure and likelihood of nodal involvement predicted by pathologic determinants has recently led to considering a selective approach to lymphadenectomy for endometrial cancer. Therefore, it became critical to examine reproducibility of diagnosing the key determinants of risk, on preoperative endometrial tissue samples as well as the concordance between preoperative and postresection specimens. Six gynaecologic pathologists assessed 105 consecutive endometrial biopsies originally reported as positive for endometrial cancer for cell type (endometrioid versus nonendometrioid), tumor grade (FIGO 3-tiered and 2-tiered), nuclear grade, and risk category (low risk defined as endometrioid histology, grade 1 + 2 and nuclear grade <3). Interrater agreement levels were substantial for identification of nonendometrioid histology (κ = 0.63; SE = 0.025), high tumor grade (κ = 0.64; SE = 0.025), and risk category (κ = 0.66; SE = 0.025). The overall agreement was fair for nuclear grade (κ = 0.21; SE = 0.025). There is agreement amongst pathologists in identifying high-risk pathologic determinants on endometrial cancer biopsies, and these highly correlate with postresection specimens. This is ascertainment prerequisite adaptation of the paradigm shift in surgical staging of patients with endometrial cancer