Overcoming the false-minima problem in direct methods: Structure determination of the packaging enzyme P4 from bacteriophage φ13

The problems encountered during the phasing and structure determination of the packaging enzyme P4 from bacteriophage φ13 using the anomalous signal from selenium in a single-wavelength anomalous dispersion experiment (SAD) are described. The oligomeric state of P4 in the virus is a hexamer (with sixfold rotational symmetry) and it crystallizes in space group C2, with four hexamers in the crystallographic asymmetric unit. Current state-of-the-art ab initio phasing software yielded solutions consisting of 96 atoms arranged as sixfold symmetric clusters of Se atoms. However, although these solutions showed high correlation coefficients indicative that the substructure had been solved, the resulting phases produced uninterpretable electron-density maps. Only after further analysis were correct solutions found (also of 96 atoms), leading to the eventual identification of the positions of 120 Se atoms. Here, it is demonstrated how the difficulties in finding a correct phase solution arise from an intricate false-minima problem. © 2005 International Union of Crystallography - all rights reserved

Microwave Absorption of Surface-State Electrons on Liquid 3^3He

We have investigated the intersubband transitions of surface state electrons (SSE) on liquid $^3$He induced by microwave radiation at temperatures from 1.1 K down to 0.01 K. Above 0.4 K, the transition linewidth is proportional to the density of $^3$He vapor atoms. This proportionality is explained well by Ando's theory, in which the linewidth is determined by the electron - vapor atom scattering. However, the linewidth is larger than the calculation by a factor of 2.1. This discrepancy strongly suggests that the theory underestimates the electron - vapor atom scattering rate. At lower temperatures, the absorption spectrum splits into several peaks. The multiple peak structure is partly attributed to the spatial inhomogeneity of the static holding electric field perpendicular to the electron sheet.Comment: 15 pages, 7 figures, submitted to J. Phys. Soc. Jp

Crew Motion and the Dynamic Environment of Spaceborne Experiments

Analytical study of crew motion on dynamic environment of orbiting laboratorie

Probing the extreme realm of AGN feedback in the massive galaxy cluster, RX J1532.9+3021

We present a detailed Chandra, XMM-Newton, VLA and HST analysis of one of the strongest cool core clusters known, RX J1532.9+3021 (z=0.3613). Using new, deep 90 ks Chandra observations, we confirm the presence of a western X-ray cavity or bubble, and report on a newly discovered eastern X-ray cavity. The total mechanical power associated with these AGN-driven outflows is (22+/-9)*10^44 erg/s, and is sufficient to offset the cooling, indicating that AGN feedback still provides a viable solution to the cooling flow problem even in the strongest cool core clusters. Based on the distribution of the optical filaments, as well as a jet-like structure seen in the 325 MHz VLA radio map, we suggest that the cluster harbours older outflows along the north to south direction. The jet of the central AGN is therefore either precessing or sloshing-induced motions have caused the outflows to change directions. There are also hints of an X-ray depression to the north aligned with the 325 MHz jet-like structure, which might represent the highest redshift ghost cavity discovered to date. We further find evidence of a cold front (r=65kpc) that coincides with the outermost edge of the western X-ray cavity and the edge of the radio mini-halo. The common location of the cold front with the edge of the radio mini-halo supports the idea that the latter originates from electrons being reaccelerated due to sloshing induced turbulence. Alternatively, its coexistence with the edge of the X-ray cavity may be due to cool gas being dragged out by the outburst. We confirm that the central AGN is highly sub-Eddington and conclude that a >10^10M_Sun or a rapidly spinning black hole is favoured to explain both the radiative-inefficiency of the AGN and the powerful X-ray cavities.Comment: Accepted for publication to ApJ (minor corrections), 16 pages, 16 figures, 5 tables. Full resolution at http://www.stanford.edu/~juliehl/M1532

Receiving Stocker Cattle Performance is Similar With Either Corn or Sorghum Wet Distillers Grains

Distillers grains are an excellent energy and protein feed source for beef cattle. Corn distillers grains have been fed to beef cattle for many years, but sorghum distillers grains are becoming more popular and may be more cost effective than corn. Sorghum is very comparable to corn in terms of energy, but has a higher crude protein value. All distillers grains are available in a wet and dry form. The moist texture of wet distillers grains can help to reduce sorting at the bunk and appears to improve intake in young calves. The objective of this study was to evaluate the effect of corn and sorghum wet distillers grains on performance and digestibility of receiving stocker calves

Evaluation of the Productivity of a Single Subcutaneous Injection of LongRange in Stocker Calves Compared With a Positive (Dectomax) and a Negative (Saline) Control

Subclinical parasitism is commonly observed in stocker cattle. Treatment of internal parasites helps to improve weight gains, feed conversion, and immune status and decreases morbidity and mortality of beef cattle (Hawkins, 1993). Some of the most concerning classes of internal parasites include Cooperia, Haemonchus, and Ostertagia. Commonly used anthelmintics come in the form of pour-ons, oral drenches, and subcutaneous injections. A majority of these drugs are designed to be administered in a single dose and provide defense against stomach worms for approximately 14 to 42 days, but the typical grazing season lasts for approximately 120 days. For grazing cattle to have season-long protection from parasites, they may require a second dose of anthelmintic treatment, which would require cattle to be gathered and processed through a chute in the middle of the grazing season. LongRange (Merial, Duluth, GA) is the first single-dose extended release anthelmintic that provides approximately 100 to 150 days of protection. This is accomplished by combining two forms of the active ingredient: one that is released into the blood immediately after injection and a second that consists of a slow-release polymer that releases the active ingredient gradually throughout the grazing period. The objective of this study was to measure body weight productivity, fecal egg counts, and fly repellent capabilities of LongRange when administered once subcutaneously at 1.0 mg/kg body weight as a long-acting solution compared with a commercially available injectable (Dectomax; Zoetis, Florham Park, NJ) and saline in stocker cattle

The Determination of Nuclear Level Densities from Experimental Information -

A novel Information Theory based method for determining the density of states from prior information is presented. The energy dependence of the density of states is determined from the observed number of states per energy interval and model calculations suggest that the method is sufficiently reliable to calculate the thermal properties of nuclei over a reasonable temperature range.Comment: 7 pages + 6 eps figures, REVTEX 3.

Microwave saturation of the Rydberg states of electrons on helium

We present measurements of the resonant microwave excitation of the Rydberg energy levels of surface state electrons on superfluid helium. The temperature dependent linewidth agrees well with theoretical predictions and is very small below 300 mK. Absorption saturation and power broadening were observed as the fraction of electrons in the first excited state was increased to 0.49, close to the thermal excitation limit of 0.5. The Rabi frequency was determined as a function of microwave power. The high values of the ratio of the Rabi frequency to linewidth confirm this system as an excellent candidate for creating qubits.Comment: 4 pages, 4 figure

Level density of 56^{56}Fe and low-energy enhancement of γ\gamma-strength function

The $^{55}$Mn$(d,n)^{56}$Fe differential cross section is measured at $E_d=7$ MeV\@. The $^{56}$Fe level density obtained from neutron evaporation spectra is compared to the level density extracted from the $^{57}$Fe$(^3$He,$\alpha\gamma)^{56}$Fe reaction by the Oslo-type technique. Good agreement is found between the level densities determined by the two methods. With the level density function obtained from the neutron evaporation spectra, the $^{56}$Fe $\gamma$-strength function is also determined from the first-generation $\gamma$ matrix of the Oslo experiment. The good agreement between the past and present results for the $\gamma$-strength function supports the validity of both methods and is consistent with the low-energy enhancement of the $\gamma$ strength below $\sim 4$ MeV first discovered by the Oslo method in iron and molybdenum isotopes.Comment: 7 pages, 5 figure