Archaeoentomological Perspectives on Dorset Occupations in Newfoundland: A Case Study from the Site of Phillip’s Garden (EeBi-1)

The Dorset Paleo-Inuit occupied the island of Newfoundland for over 800 years (1990 BP to 1180 BP), producing a unique and identifiable archaeological record. Despite this, our current understanding of this sea-mammal hunting population is essentially based on past analysis of architecture, artefacts, and animal remains. While these data show that the Dorset intensively used sites at different locations through the Arctic and Subarctic, very little information is known regarding their impact on their surroundings and the environment. Our research is based on soil samples collected at the site of Phillip’s Garden, which is considered to be one of the most intensely occupied sites within the Dorset culture’s geographic range. Beetles, which are known to be ecological specialists, were used as a proxy in this research in order to document the composition and fluctuation in vegetation, and environmental change through time at the site. The analysis demonstrates that the Dorset communities of Phillip’s Garden harvested trees, and modified the environment and the flora surrounding their settlement more intensively than previously thought.Entre 1990 AA et 1180 AA, l’île de Terre-Neuve a connu une période d’occupation de plus de 800 ans par la culture dorsétienne. Cette occupation, documentée dans les données archéologiques, a laissé des traces uniques et visibles. Malgré cette richesse archéologique laissée par les Dorsétiens, notre compréhension de cette culture axée sur la chasse aux mammifères marins est principalement basée sur les données architecturales, artéfactuelles et zooarchéologiques. Ces données ont permis de démontrer que la culture dorsétienne a occupé de façon très intensive plusieurs sites localisés dans l’Arctique et le Subarctique. Cependant, peu d’informations sont fournies par ces données quant à l’impact de cette culture sur son environnement et ses lieux d’occupation. Les données utilisées au cours de notre recherche ont été récupérées dans des échantillons de sols ayant été prélevés sur le site de Phillip’s Garden, considéré comme l’un des sites les plus intensément occupés par la culture dorsétienne. En tant que spécialistes de leur niche écologique, les coléoptères ont été utilisés dans cette recherche afin de documenter la composition et les modifications visibles dans la végétation du site à travers le temps. Cette analyse permet de changer notre perception de l’étendue des modifications faites à la flore et à l’environnement par les Dorsétiens établis au site de Phillip’s Garden, modifications dues entre autres à l’exploitation des arbres

Reconstructing caribou seasonal biogeography in Little Ice Age (late Holocene) Western Alaska using intra-tooth strontium and oxygen isotope analysis

Acknowledgements This work was funded by an Arts and Humanities Research Council (AH/K006029/1) grant awarded to Rick Knecht, Kate Britton and Charlotta Hillerdal (Aberdeen). The onsite collection of samples was carried out by staff and students from the University of Aberdeen, volunteer excavators and the residents of Quinhagak. We also thank the Qanirtuuq Incorporated, Quinhagak, Alaska, and the people of Quinhagak for logistical and planning support for fieldwork and sampling permissions. Special thanks for Warren Jones and Qanirtuuq Incorporated (especially Michael Smith and Lynn Church), and to all Nunalleq project team members, particularly Edouard Masson-MacLean and Paul Ledger (Aberdeen). Thanks also to Alison Harris (Stockholm/York), Rebecca Lam and Sherri Strong (Memorial University) and Michael Maus (Johannes-Gutenberg Universität, Mainz) for methodological and technical assistance. Thank you to Dominic Demma (Alaska Department of Fish and Game) for providing information on the Mulchatna herd, to Edouard Masson-MacLean for comments on an earlier draft of this manuscript, and to the guest editors for inviting us to contribute to this special issue. Finally, the authors would like to thank the reviewers for their valuable and constructive comments on this manuscript.Peer reviewedPublisher PD

On the hoof:exploring the supply of animals to the Roman legionary fortress at Caerleon using strontium (⁸⁷Sr/⁸⁶Sr) isotope analysis

Provisioning large concentrations of professional soldiers in Britain after the invasion in AD 43 was a major challenge for the Roman imperial administration. In a distant frontier province such as Britannia, it is generally believed that locally produced agricultural resources must have been vital in feeding and maintaining the occupying army, but direct evidence for this is limited. This study explores the potential for strontium isotope analysis (87Sr/86Sr) of faunal dental enamel to provide new insights for the provisioning of animals to the legionary fortress at Caerleon in South East Wales. Results from 37 domestic animals (cattle, caprines and pigs) from the recent excavation of a military store building in Caerleon are presented. Thirteen faunal dentine and modern plant samples were also analysed to characterise baseline signals for the local landscape. These results indicated an unexpectedly wide range of bioavailable 87Sr/86Sr in the local area, meaning identifying allochthonous individuals with confidence is difficult. The faunal dataset is also very wide ranging, but at least seven individuals can be identified as originating from beyond the local region, with some likely being brought from a substantial distance away. It is highly likely, however, that this underestimates the proportion of animals reared outside the fortress’ immediate hinterland and individuals that fall within the diverse local range could also have been from further afield. This has implications for understanding how the Roman legion was sustained, as well as the impact that provisioning the army had on the countryside, particularly around military sites

Feasting and mobility in Iron Age Ireland: multi-isotope analysis reveals the vast catchment of Navan Fort, Ulster

Navan Fort is an iconic prehistoric Irish ceremonial centre and the legendary capital of Ulster. The fort has produced an exceptional pig-dominated faunal assemblage that also contained a barbary macaque skull. Dating from the 4th to 1st century BC, it is likely to be a ceremonial feasting centre that may have drawn people and their animals from across Ulster and beyond. This study uses a multi-isotope (87Sr/86Sr, δ34S, δ13C, δ15N) approach to identify non-local animals and reconstruct site catchment. New biosphere mapping means that isotope data can be more confidently interpreted and the combination of strontium and sulphur analysis has the potential to estimate origins. In the absence of human remains, fauna provide the best proxy for human movement. Results for the 35 analysed animals are wide-ranging, especially in terms of strontium (0.707–0.715), which has the largest range for an Irish site. Sulphur values are more restricted (13.1‰−17.1‰) but are high in the context of British and Irish data. Results provide clear evidence for animals (and thus people) coming from across Ulster and beyond, demonstrating the site’s wide catchment. Navan Fort was clearly a major ceremonial centre with far-reaching influence and hosted feasts that drew people and animals from afar

Oral vitamin D supplementation induces transcriptomic changes in rectal mucosa that are linked to anti-tumour effects

Abstract Background The risk for several common cancers is influenced by the transcriptomic landscape of the respective tissue-of-origin. Vitamin D influences in vitro gene expression and cancer cell growth. We sought to determine whether oral vitamin D induces beneficial gene expression effects in human rectal epithelium and identify biomarkers of response. Methods Blood and rectal mucosa was sampled from 191 human subjects and mucosa gene expression (HT12) correlated with plasma vitamin D (25-OHD) to identify differentially expressed genes. Fifty subjects were then administered 3200IU/day oral vitamin D3 and matched blood/mucosa resampled after 12 weeks. Transcriptomic changes (HT12/RNAseq) after supplementation were tested against the prioritised genes for gene-set and GO-process enrichment. To identify blood biomarkers of mucosal response, we derived receiver-operator curves and C-statistic (AUC) and tested biomarker reproducibility in an independent Supplementation Trial (BEST-D). Results Six hundred twenty-nine genes were associated with 25-OHD level (P < 0.01), highlighting 453 GO-term processes (FDR<0.05). In the whole intervention cohort, vitamin D supplementation enriched the prioritised mucosal gene-set (upregulated gene-set P < 1.0E−07; downregulated gene-set P < 2.6E−05) and corresponding GO terms (P = 2.90E−02), highlighting gene expression patterns consistent with anti-tumour effects. However, only 9 individual participants (18%) showed a significant response (NM gene-set enrichment P < 0.001) to supplementation. Expression changes in HIPK2 and PPP1CC expression served as blood biomarkers of mucosal transcriptomic response (AUC=0.84 [95%CI 0.66–1.00]) and replicated in BEST-D trial subjects (HIPK2 AUC=0.83 [95%CI 0.77–0.89]; PPP1CC AUC=0.91 [95%CI 0.86–0.95]). Conclusions Higher plasma 25-OHD correlates with rectal mucosa gene expression patterns consistent with anti-tumour effects, and this beneficial signature is induced by short-term vitamin D supplementation. Heterogenous gene expression responses to vitamin D may limit the ability of randomised trials to identify beneficial effects of supplementation on CRC risk. However, in the current study blood expression changes in HIPK2 and PPP1CC identify those participants with significant anti-tumour transcriptomic responses to supplementation in the rectum. These data provide compelling rationale for a trial of vitamin D and CRC prevention using easily assayed blood gene expression signatures as intermediate biomarkers of response

Phenotypic plasticity in normal breast derived epithelial cells

Background Normal, healthy human breast tissue from a variety of volunteer donors has become available for research thanks to the establishment of the Susan G. Komen for the Cure® Tissue Bank at the IU Simon Cancer Center (KTB). Multiple epithelial (K-HME) and stromal cells (K-HMS) were established from the donated tissue. Explant culture was utilized to isolate the cells from pieces of breast tissue. Selective media and trypsinization were employed to select either epithelial cells or stromal cells. The primary, non-transformed epithelial cells, the focus of this study, were characterized by immunohistochemistry, flow cytometry, and in vitro cell culture. Results All of the primary, non-transformed epithelial cells tested have the ability to differentiate in vitro into a variety of cell types when plated in or on biologic matrices. Cells identified include stratified squamous epithelial, osteoclasts, chondrocytes, adipocytes, neural progenitors/neurons, immature muscle and melanocytes. The cells also express markers of embryonic stem cells. Conclusions The cell culture conditions employed select an epithelial cell that is pluri/multipotent. The plasticity of the epithelial cells developed mimics that seen in metaplastic carcinoma of the breast (MCB), a subtype of triple negative breast cancer; and may provide clues to the origin of this particularly aggressive type of breast cancer. The KTB is a unique biorepository, and the normal breast epithelial cells isolated from donated tissue have significant potential as new research tools

Finding Mycenaeans in Minoan Crete? Isotope and DNA analysis of human mobility in Bronze Age Crete.

We undertook a large-scale study of Neolithic and Bronze Age human mobility on Crete using biomolecular methods (isotope analysis, DNA), with a particular focus on sites dating to the Late Bronze Age ('Late Minoan') period. We measured the strontium and sulphur isotope values of animal remains from archaeological sites around the island of Crete to determine the local baseline values. We then measured the strontium and sulphur values of humans from Late Neolithic and Bronze Age sites. Our results indicate that most of the humans have sulphur and strontium isotope values consistent with being local to Crete, showing no evidence for a wide-scale movement of people from the Greek mainland or other areas away from Crete in these time periods. However, we found four individuals from the late Bronze Age (Late Minoan III) cemetery of Armenoi with sulphur isotope values not typically found in Crete and are instead consistent with an origin elsewhere. This cemetery at Armenoi also has one of only a few examples of the newly adopted Mycenaean Linear B script on Crete found outside of the palace sites, pointing to an influence (trade and possible migration) from the mainland, which may then be the place of origin of these four individuals. DNA (mtDNA) studies of eight Late Bronze Age individuals from Armenoi have results consistent with people living in Aegean region at this time and cannot be used to distinguish between individuals from Crete ('Minoans') and the Greek mainland ['Mycenaeans'])

Tuning the endothelial response: differential release of exocytic cargos from Weibel-Palade bodies.

Essentials Endothelial activation initiates multiple processes, including hemostasis and inflammation. The molecules that contribute to these processes are co-stored in secretory granules. How can the cells control release of granule content to allow differentiated responses? Selected agonists recruit an exocytosis-linked actin ring to boost release of a subset of cargo. SUMMARY: Background Endothelial cells harbor specialized storage organelles, Weibel-Palade bodies (WPBs). Exocytosis of WPB content into the vascular lumen initiates primary hemostasis, mediated by von Willebrand factor (VWF), and inflammation, mediated by several proteins including P-selectin. During full fusion, secretion of this large hemostatic protein and smaller pro-inflammatory proteins are thought to be inextricably linked. Objective To determine if secretagogue-dependent differential release of WPB cargo occurs, and whether this is mediated by the formation of an actomyosin ring during exocytosis. Methods We used VWF string analysis, leukocyte rolling assays, ELISA, spinning disk confocal microscopy, high-throughput confocal microscopy and inhibitor and siRNA treatments to demonstrate the existence of cellular machinery that allows differential release of WPB cargo proteins. Results Inhibition of the actomyosin ring differentially effects two processes regulated by WPB exocytosis; it perturbs VWF string formation but has no effect on leukocyte rolling. The efficiency of ring recruitment correlates with VWF release; the ratio of release of VWF to small cargoes decreases when ring recruitment is inhibited. The recruitment of the actin ring is time dependent (fusion events occurring directly after stimulation are less likely to initiate hemostasis than later events) and is activated by protein kinase C (PKC) isoforms. Conclusions Secretagogues differentially recruit the actomyosin ring, thus demonstrating one mechanism by which the prothrombotic effect of endothelial activation can be modulated. This potentially limits thrombosis whilst permitting a normal inflammatory response. These results have implications for the assessment of WPB fusion, cargo-content release and the treatment of patients with von Willebrand disease.British Heart Foundation. Grant Number: PG/15/72/31732. Medical Research Council. Grant Numbers: MC_UU_12018/2, MR/M019179/1

The history of Coast Salish “woolly dogs” revealed by ancient genomics and Indigenous Knowledge

Ancestral Coast Salish societies in the Pacific Northwest kept long-haired "woolly dogs" that were bred and cared for over millennia. However, the dog wool-weaving tradition declined during the 19th century, and the population was lost. In this study, we analyzed genomic and isotopic data from a preserved woolly dog pelt from "Mutton," collected in 1859. Mutton is the only known example of an Indigenous North American dog with dominant precolonial ancestry postdating the onset of settler colonialism. We identified candidate genetic variants potentially linked with their distinct woolly phenotype. We integrated these data with interviews from Coast Salish Elders, Knowledge Keepers, and weavers about shared traditional knowledge and memories surrounding woolly dogs, their importance within Coast Salish societies, and how colonial policies led directly to their disappearance