210 research outputs found
The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis
peer-reviewedThe large pine weevil Hylobius abietis L. is a major forestry pest in 15 European countries, where it is a threat to 3.4 million hectares of forest. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of H. abietis was performed. Injection with Metarhizium brunneum or Beauvaria bassiana culture filtrate facilitated a significantly increased yeast cell proliferation in larvae. Larvae co-injected with either Beauvaria caledonica or B. bassiana culture filtrate and Candida albicans showed significantly increased mortality. Together these results suggest that EPF culture filtrate has the potential to modulate the insect immune system allowing a subsequent pathogen to proliferate. Injection with EPF culture filtrate was shown to alter the abundance of protease inhibitors, detoxifing enzymes, antimicrobial peptides and proteins involved in reception/detection and development in H. abietis larvae. Larvae injected with B. caledonica culture filtrate displayed significant alterations in abundance of proteins involved in cellulolytic and other metabolic processes in their haemolymph proteome. Screening EPF for their ability to modulate the insect immune response represents a means of assessing EPF for use as biocontrol agents, particularly if the goal is to use them in combination with other control agents.This research was funded by the Irish Government (Department of Agriculture, Food and the Marine) (10/RD/MCOP/NUIM/720) under the National Development Plan 2007–2013 and through the MU Department of Biology Contingency Fund. The Q-Exactive quantitative mass spectrometer was funded under the SFI Research Infrastructure Call 2012; Grant Number: 12/RI/2346 (3) to Prof. S. Doyle
Application of hierarchical clustering to identify high risk pests to Sitka spruce: Ireland as a case study
Invertebrate forest pests and pathogens can cause considerable economic losses and modern patterns of trade
have facilitated the international movement of pest species on an unprecedented level. This upsurge in trade has
increased the pathways available to high risk species, facilitating entry and potential establishment in nations
where they were previously absent. To support policy and pest prioritization, pest risk analyses are conducted
to decide ‘if’ and ‘how’ pests should be regulated in order to prevent entry or establishment; however, they
cannot be carried out for every potential pest. This paper utilizes a hierarchical clustering (HC) approach to
analyse distribution data for pests of Sitka spruce (Picea sitchensis (Bong.) Carr.) in order to identify species of
high risk to Ireland, as well as potential source regions of these pests. The presence and absence of almost a
1000 pests across 386 regions globally are clustered based on their similarity of pest assemblages, to provide an
objective examination of the highest risk pests to Irish forestry. Regional clusters were produced for each taxon
analysed including the Coleoptera, Diptera, Hemiptera, Hymenoptera, Nematoda, Lepidoptera and the Fungi. The
results produced by the HC analysis were interpreted with regard to biological realism and climate. Biologically
meaningful clusters were produced for each of the groups, except for the Diptera and Nematoda, and each of
the species analysed were ranked within their group by a quantitative risk index specific to the island of Ireland.
The impact of uncertainty in the distribution data is also examined, in order to assess its influence over the final
groupings produced. The outputs from this analysis suggest that the highest risk pests for Ireland’s Sitka spruce
plantations will originate from within Europe. Ultimately, Ireland could benefit from seeking regulation for some
of the higher ranking pests identified in this analysis. This analysis provides the first of its type for Sitka spruce, as well as its application in Ireland. It also serves to highlight the potential utility of HC as a ‘first approach’ to assessing the risk posed by alien species to hitherto novel regions
Inundative pest control: How risky is it? A case study using entomopathogenic nematodes in a forest ecosystem
Entomopathogenic nematodes (EPN) are globally important inundative biological control agents. Their widespread use makes environmental risk assessment important, but very few comprehensive post-application risk assessments have been conducted for EPN. We apply a rigorous risk analysis procedure to the use of EPN applied in a forest ecosystem to suppress the large pine weevil (Hylobius abietis). In this synthesis, we provide a quantitative evaluation of five risk categories: (a) establishment, (b) dispersal, (c) host range, (d) direct non-target effects and (e) indirect non-target effects. A low level of risk was identified (35–51 out of a possible total of 125). Species exotic to the clear-fell forest ecosystem (Steinernema carpocapsae and Heterorhabditis downesi) were accorded a lower overall risk status than native species and strains (Steinernema feltiae), largely as a result of their shorter persistence in the target environment. We conclude that EPN are a low risk viable alternative control for pine weevil compared to the higher risk conventional control using pyrethroid or neonicotinoid insecticides
Objective and subjective components of resource value in lethal fights between male entomopathogenic nematodes
Males sometimes engage in fights over contested resources such as access to mates; in this case, fighting behaviour may be adjusted based on the value they place on the females. Resource value (RV) can have two components. First, males can assess the quality of females, which constitutes an objective assessment of RV. Second, internal state such as previous mating experience can also influence motivation to fight thus constituting a subjective assessment of RV. If mating opportunities are scarce and available females have a major impact on the lifetime reproductive success of males, then fighting can be fatal; in this situation it is uncertain whether males would adjust fighting behaviour based on RV. We found that both female quality, that is, virginity (objective component of RV) and male mating status (subjective component of RV) influenced fighting intensity between males of the entomopathogenic nematode Steinernema longicaudum which engage in lethal fights. Male nematodes were more likely to engage in fighting and fought longer and more frequently in the presence of virgin (high-quality) females than in the presence of mated (lower-quality) females. Male mating status was also found to influence fighting behaviour: mated males were the winners in staged fights between mated and virgin males. Mated males may have superior fighting ability (greater resource-holding potential), but RV asymmetries between mated and virgin males cannot be excluded. Males were more likely to win when they were resident, but we did not find a significant interaction effect between male mating and residency status
Low-temperature exposure has immediate and lasting effects on the stress tolerance, chemotaxis and proteome of entomopathogenic nematodes
Temperature is one of the most important factors affecting soil organisms, including the infective stages of parasites and entomopathogenic nematodes, which are important biological control agents. We investigated the response of 2 species of entomopathogenic nematodes to different storage regimes: cold (9°C), culture temperature (20°C) and temperature swapped from 9 to 20°C. For Steinernema carpocapsae, cold storage had profound effects on chemotaxis, stress tolerance and protein expression that were retained in temperature-swapped individuals. These effects included reversal of chemotactic response for 3 (prenol, methyl salicylate and hexanol) of the 4 chemicals tested, and enhanced tolerance to freezing (−10°C) and desiccation (75% RH). Label-free quantitative proteomics showed that cold storage induced widespread changes in S. carpocapsae, including an increase in heat-shock proteins and late embryogenesis abundant proteins. For Heterorhabditis megidis, cold storage had a less dramatic effect on chemotaxis (as previously shown for proteomic expression) and changes were not maintained on return to 20°C. Thus, cold temperature exposure has significant effects on entomopathogenic nematodes, but the nature of the change depends on the species. Steinernema carpocapsae, in particular, displays significant plasticity, and its behaviour and stress tolerance may be manipulated by brief exposure to low temperatures, with implications for its use as a biological control agent
phenModel: A temperature-dependent phenology/voltinism model for a herbivorous insect incorporating facultative diapause and budburst
A comprehensive phenology/voltinism model was developed for Phratora vulgatissima, an important pest of
bioenergy crops. The model, phenModel, was developed based on development times of different life cycle stages
(eggs, larvae, pupae, pre-oviposition, oviposition, sexual maturation) obtained from constant temperature laboratory experiments. As part of this study, a number of linear and non-linear models which describe the
temperature-dependent development rate (inverse of development time) for each of the different life cycle stages were fitted. Based on the criteria of model parsimony and model fit, the non-linear Lactin-2 model was chosen as
the optimum model to describe temperature-driven development in P. vulgatissima. To account for the variation
in development times between individuals, an important but often ignored aspect in phenology models, a
number of stochastic models (2- and 3- parameter Weibull and logistic models) were evaluated, based on the
assumption that normalised development times conform to a similar shaped ('same shape') distribution. Novel
aspects of the phenology model include the incorporation of a biologically relevant biofix, based on a budburst
model for Salix viminalis, and a photoperiod threshold to induce facultative diapause. The model, which is
written in R for accessibility, requires inputs of daily minimum and maximum temperature and site latitude and
produces outputs describing the timing of completion of developmental stages for specified proportions of the
population. It was evaluated against available field data and found to largely reproduce the observations providing a measure of its potential utility. A key component of the model allows for a sensitivity analysis of the
model parameters. The model is structured so that it can easily be adapted for other leaf-feeding beetles which
display a facultative reproductive diapause cued by photoperiod, and where the onset of oviposition is dependent on budburst, assuming relevant life cycle stage parameters are available
Comment Re: Non-Compete Clause Rulemaking, Matter No. P201200
Within signed law professors and law students submitted this letter to the Federal Trade Commission, writing in their individual capacities, not as agents of their affiliated institutions, in support of the Federal Trade Commission’s proposed rule to ban most non-compete clauses (the “Proposal”) as an unfair method of competition.
This letter offers comments in response to areas where the FTC has requested public comment. To make our views clear, this letter contains the following sections:
I. Summary of the Proposal;
II. The Commission Should Consider Expanding Its Definition of Non-Compete Clauses to Prevent Employers from Requiring Workers to Quit Before Seeking Alternative Employment;
III. Non-Compete Clauses Are Unfair Methods of Competition;
IV. Non-Compete Clauses Negatively Impact Workers and Their Families;
V. The Proposed Rule Protects Small Businesses and Entrepreneurs; and
VI. The Commission Should Consider a Factor Test for Its Unfairness Analysis for Senior Executive
Retrospective harm benefit analysis of pre-clinical animal research for six treatment interventions
The harm benefit analysis (HBA) is the cornerstone of animal research regulation and is considered to be a key ethical safeguard for animals. The HBA involves weighing the anticipated benefits of animal research against its predicted harms to animals but there are doubts about how objective and accountable this process is.i. To explore the harms to animals involved in pre-clinical animal studies and to assess these against the benefits for humans accruing from these studies; ii. To test the feasibility of conducting this type of retrospective HBA.Data on harms were systematically extracted from a sample of pre-clinical animal studies whose clinical relevance had already been investigated by comparing systematic reviews of the animal studies with systematic reviews of human studies for the same interventions (antifibrinolytics for haemorrhage, bisphosphonates for osteoporosis, corticosteroids for brain injury, Tirilazad for stroke, antenatal corticosteroids for neonatal respiratory distress and thrombolytics for stroke). Clinical relevance was also explored in terms of current clinical practice. Harms were categorised for severity using an expert panel. The quality of the research and its impact were considered. Bateson's Cube was used to conduct the HBA.The most common assessment of animal harms by the expert panel was 'severe'. Reported use of analgesia was rare and some animals (including most neonates) endured significant procedures with no, or only light, anaesthesia reported. Some animals suffered iatrogenic harms. Many were kept alive for long periods post-experimentally but only 1% of studies reported post-operative care. A third of studies reported that some animals died prior to endpoints. All the studies were of poor quality. Having weighed the actual harms to animals against the actual clinical benefits accruing from these studies, and taking into account the quality of the research and its impact, less than 7% of the studies were permissible according to Bateson's Cube: only the moderate bisphosphonate studies appeared to minimise harms to animals whilst being associated with benefit for humans.This is the first time the accountability of the HBA has been systematically explored across a range of pre-clinical animal studies. The regulatory systems in place when these studies were conducted failed to safeguard animals from severe suffering or to ensure that only beneficial, scientifically rigorous research was conducted. Our findings indicate a pressing need to: i. review regulations, particularly those that permit animals to suffer severe harms; ii. reform the processes of prospectively assessing pre-clinical animal studies to make them fit for purpose; and iii. systematically evaluate the benefits of pre-clinical animal research to permit a more realistic assessment of its likely future benefits
Infectivity of Plasmodium falciparum in malaria-naive individuals is related to knob expression and cytoadherence of the parasite
Plasmodium falciparum is the most virulent human malaria parasite because of its ability to cytoadhere in the microvasculature. Nonhuman primate studies demonstrated relationships among knob expression, cytoadherence, and infectivity. This has not been examined in humans. Cultured clinical-grade P. falciparum parasites (NF54, 7G8, and 3D7B) and ex vivo-derived cell banks were characterized. Knob and knob-associated histidine-rich protein expression, CD36 adhesion, and antibody recognition of parasitized erythrocytes (PEs) were evaluated. Parasites from the cell banks were administered to malaria-naive human volunteers to explore infectivity. For the NF54 and 3D7B cell banks, blood was collected from the study participants for in vitro characterization. All parasites were infective in vivo. However, infectivity of NF54 was dramatically reduced. In vitro characterization revealed that unlike other cell bank parasites, NF54 PEs lacked knobs and did not cytoadhere. Recognition of NF54 PEs by immune sera was observed, suggesting P. falciparum erythrocyte membrane protein 1 expression. Subsequent recovery of knob expression and CD36-mediated adhesion were observed in PEs derived from participants infected with NF54. Knobless cell bank parasites have a dramatic reduction in infectivity and the ability to adhere to CD36. Subsequent infection of malaria-naive volunteers restored knob expression and CD36-mediated cytoadherence, thereby showing that the human environment can modulate virulence
Local host-dependent persistence of the entomopathogenic nematode Steinernema carpocapsae used to control the large pine weevil Hylobius abietis
Entomopathogenic nematodes (EPN) applied inundatively to suppress insect pests are more likely to persist and establish in stable agroecosystems than in annual crops. We investigated a system of intermediate stability: three stumps harbouring the large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae), a major European forestry pest. We tested whether persistence of EPN Steinernema carpocapsae Weiser (Rhabditida: Steinernematidae) applied around stumps is maintained by recycling of EPN through pine weevils developing within stumps. Steinernema carpocapsae was detected in soil around and under the bark of treated tree stumps up to two years, but not 4–5 years after application. Differences in nematode presence between sites were better explained by tree species (pine or spruce) than soil type (mineral or peat). Presence of S. carpocapsae in soil was positively correlated with the number of H. abietis emerging from untreated stumps the previous year, which was greater for pine stumps than spruce stumps
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